avidin biotin activity  (Vector Laboratories)


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    Name:
    Avidin Biotin Blocking Kit
    Description:
    Avidin Biotin Blocking Kit blocks all endogenous biotin biotin receptors and avidin binding sites present in tissues This kit is designed for use with avidin detection systems such as the VECTASTAIN ABC Kits if avidin or biotinylated reagents bind non specifically to tissues or proteins This blocking kit consists of 18 ml of Avidin D and 18 ml of biotin in convenient dropper bottles
    Catalog Number:
    sp-2001
    Price:
    None
    Size:
    1 Kit
    Category:
    Histology reagents or solutions or stains
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    Structured Review

    Vector Laboratories avidin biotin activity
    Avidin Biotin Blocking Kit
    Avidin Biotin Blocking Kit blocks all endogenous biotin biotin receptors and avidin binding sites present in tissues This kit is designed for use with avidin detection systems such as the VECTASTAIN ABC Kits if avidin or biotinylated reagents bind non specifically to tissues or proteins This blocking kit consists of 18 ml of Avidin D and 18 ml of biotin in convenient dropper bottles
    https://www.bioz.com/result/avidin biotin activity/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin activity - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: In Vivo and In Vitro Evidence for Placental DNA Damage in Preeclampsia
    Article Snippet: .. For immunohistochemistry (IHC) and immunocytochemistry (ICC), avidin-blocking kit, avidin-biotin-peroxidase kit with 3,3-diaminobenzidine tetrahydrochloride dehydrate (DAB) and Vectashield mounting medium with DAPI (H-1500) were purchased from Vector Laboratories (Burlingame, CA). .. For RT-qPCR, pre-validated human primers were from TaqMan Assay-On-Demand (Applied Biosystems, Foster City, CA), including γH2AX (Hs00266783_s1) and 18S (Hs99999901_s1).

    Amplification:

    Article Title: Effects of Estrogen Therapy on the Serotonergic System in an Animal Model of Perimenopause Induced by 4-Vinylcyclohexen Diepoxide (VCD)
    Article Snippet: .. Signal amplification was performed using an avidin-biotin kit (1:100; Vector Laboratories) for 1 h at room temperature. ..

    Avidin-Biotin Assay:

    Article Title: Complex Tissue Regeneration in Mammals Is Associated With Reduced Inflammatory Cytokines and an Influx of T Cells
    Article Snippet: .. For fluorescence-microscopy, antigens were exposed by heat mediated retrieval with sodium citrate buffer, pH = 6.0, resident avidin and biotin was blocked (Vector Laboratories, SP-2001). .. Sections were then blocked with 2.5% horse serum, incubated with primary antibody (rabbit anti-CD3, DAKO, Cat#A0452, 1:500) overnight at 4°C, incubated with a biotin conjugated, secondary antibody (goat anti-rabbit, Vector Laboratories, Cat#PK-6101, 1:400) for 1 h at room temp, incubated with streptavidin conjugated AlexaFlour-594 (Molecular Probes, Cat#S11227, 1:5,000), counter-stained with 4′,6-Diamidino-2-Phenylindole, Dihydrochloride (Molecular Probes, Cat#D1306, 1:10,000) and cover-slipped.

    Article Title: In Vivo and In Vitro Evidence for Placental DNA Damage in Preeclampsia
    Article Snippet: .. For immunohistochemistry (IHC) and immunocytochemistry (ICC), avidin-blocking kit, avidin-biotin-peroxidase kit with 3,3-diaminobenzidine tetrahydrochloride dehydrate (DAB) and Vectashield mounting medium with DAPI (H-1500) were purchased from Vector Laboratories (Burlingame, CA). .. For RT-qPCR, pre-validated human primers were from TaqMan Assay-On-Demand (Applied Biosystems, Foster City, CA), including γH2AX (Hs00266783_s1) and 18S (Hs99999901_s1).

    Article Title: Oncogene-dependent function of BRG1 in hepatocarcinogenesis
    Article Snippet: .. After a 20 minutes cool down at room temperature, the slides were blocked using 5% goat serum and Avidin-Biotin blocking kit (Vector Laboratories, Burlingame, CA). .. The following primary antibodies were used: anti-Ki-67 (MA5-14520, Thermo Fisher Scientific) and anti-Brg1 (110641, Abcom).

    Article Title: Amyloid-Peptide Vaccinations Reduce ?-Amyloid Plaques but Exacerbate Vascular Deposition and Inflammation in the Retina of Alzheimer's Transgenic Mice
    Article Snippet: .. The avidin-biotin complex kit for diaminobenzidine (DAB) staining and VectaShield fluorescent mounting medium containing 4′,6′-diamidino- 2-phenylindole were purchased from Vector Laboratories (Burlingame, CA). ..

    Article Title: Effects of Estrogen Therapy on the Serotonergic System in an Animal Model of Perimenopause Induced by 4-Vinylcyclohexen Diepoxide (VCD)
    Article Snippet: .. Signal amplification was performed using an avidin-biotin kit (1:100; Vector Laboratories) for 1 h at room temperature. ..

    Blocking Assay:

    Article Title: Ultrastructural Visualization of 3D Chromatin Folding Using Serial Block-Face Scanning Electron Microscopy and In Situ Hybridization (3D-EMISH)
    Article Snippet: .. After blocking of endogenous biotin (Vector Laboratories; SP-2001), the cells were permeabilized with 0.5% Triton X-100 (Sigma) in PBS (RT, 20 min). .. To augment permeabilization, the cells were immersed in a cryoprotectant solution (20% glycerol in PBS, RT, 2h) followed by their repeated freezing-thawing above the surface of the liquid nitrogen (4 × 30 sec).

    Article Title: Oncogene-dependent function of BRG1 in hepatocarcinogenesis
    Article Snippet: .. After a 20 minutes cool down at room temperature, the slides were blocked using 5% goat serum and Avidin-Biotin blocking kit (Vector Laboratories, Burlingame, CA). .. The following primary antibodies were used: anti-Ki-67 (MA5-14520, Thermo Fisher Scientific) and anti-Brg1 (110641, Abcom).

    Article Title: Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization
    Article Snippet: .. In details, after blocking of endogenous biotin (Vector Laboratories; SP-2001), the cells were permeabilized with 0.5% Triton X-100 (Sigma) in PBS (RT, 20 min). ..

    Incubation:

    Article Title: Abdominal and Pelvic Organ Failure Induced by Intraperitoneal Influenza A Virus Infection in Mice
    Article Snippet: .. Catalog No: SP-2001), and normal horse serum (Vector Laboratories, Catalog No: S-2000), each for 15 min. Then, each slide was incubated with rabbit anti-cleaved caspase-3 antibody (Cell Signaling Technology, Danvers, MA, United States, Catalog No: 9661S) for 2 h. The slides were then washed and incubated with biotinylated horse anti-rabbit antibody (Vector Laboratories, Catalog No: BP 1100) for 30 min. .. Following incubation, the slides were washed and treated with HRP-streptavidin (Vector Laboratories, Catalog No: PK-6100).

    Immunocytochemistry:

    Article Title: In Vivo and In Vitro Evidence for Placental DNA Damage in Preeclampsia
    Article Snippet: .. For immunohistochemistry (IHC) and immunocytochemistry (ICC), avidin-blocking kit, avidin-biotin-peroxidase kit with 3,3-diaminobenzidine tetrahydrochloride dehydrate (DAB) and Vectashield mounting medium with DAPI (H-1500) were purchased from Vector Laboratories (Burlingame, CA). .. For RT-qPCR, pre-validated human primers were from TaqMan Assay-On-Demand (Applied Biosystems, Foster City, CA), including γH2AX (Hs00266783_s1) and 18S (Hs99999901_s1).

    Fluorescence Microscopy:

    Article Title: Complex Tissue Regeneration in Mammals Is Associated With Reduced Inflammatory Cytokines and an Influx of T Cells
    Article Snippet: .. For fluorescence-microscopy, antigens were exposed by heat mediated retrieval with sodium citrate buffer, pH = 6.0, resident avidin and biotin was blocked (Vector Laboratories, SP-2001). .. Sections were then blocked with 2.5% horse serum, incubated with primary antibody (rabbit anti-CD3, DAKO, Cat#A0452, 1:500) overnight at 4°C, incubated with a biotin conjugated, secondary antibody (goat anti-rabbit, Vector Laboratories, Cat#PK-6101, 1:400) for 1 h at room temp, incubated with streptavidin conjugated AlexaFlour-594 (Molecular Probes, Cat#S11227, 1:5,000), counter-stained with 4′,6-Diamidino-2-Phenylindole, Dihydrochloride (Molecular Probes, Cat#D1306, 1:10,000) and cover-slipped.

    Staining:

    Article Title: Amyloid-Peptide Vaccinations Reduce ?-Amyloid Plaques but Exacerbate Vascular Deposition and Inflammation in the Retina of Alzheimer's Transgenic Mice
    Article Snippet: .. The avidin-biotin complex kit for diaminobenzidine (DAB) staining and VectaShield fluorescent mounting medium containing 4′,6′-diamidino- 2-phenylindole were purchased from Vector Laboratories (Burlingame, CA). ..

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    Vector Laboratories endogenous peroxidase activity
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