avian myeloblastosis virus reverse transcriptase  (Stratagene)

 
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    Structured Review

    Stratagene avian myeloblastosis virus reverse transcriptase
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase/product/Stratagene
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars

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    Related Articles

    Sequencing:

    Article Title: Carbon Catabolite Repression in Lactobacillus pentosus: Analysis of the ccpA Region
    Article Snippet: .. Primer extension experiments were performed with avian myeloblastosis virus reverse transcriptase (Stratagene) and oligonucleotide LPE27 (5′-AATTGTTACTGTTTGCTTTTCC-3′), which is complementary to positions 24 to 3 of the ccpA coding sequence (see Fig. A). .. Oligonucleotides were 5′ labelled by the use of T4 polynucleotide kinase (New England Biolabs).

    Concentration Assay:

    Article Title: Reverse Transcription-PCR Detection of Mycobacterium leprae in Clinical Specimens
    Article Snippet: .. RNA (2 μg) was transcribed into cDNA by using avian myeloblastosis virus reverse transcriptase (Stratagene, La Jolla, Calif.) in a 20-μl reaction volume containing 50 mM Tris-HCl (pH 8.5), 8 mM MgCl2 , 30 mM KCl, 6 mM dithiothreitol, a 0.25 mM concentration of each deoxynucleoside triphosphate, 1 nM synthetic oligo(dT)15, 1 nM random hexamers, 1 nM P3 primer, and 400 U of RNase inhibitor (Stratagene) at 42°C for 50 min. .. The mixture was then heated to inactivate the enzymes, cooled, and diluted to 100 μl with sterile RNase-free distilled H2 O before it was added to the PCR mixture.

    Generated:

    Article Title: Dissociation of Rabies Virus Matrix Protein Functions in Regulation of Viral RNA Synthesis and Virus Assembly
    Article Snippet: .. The first-strand cDNA was generated from 1 μg of RNA by using avian myeloblastosis virus reverse transcriptase (Stratagene). cDNA was PCR amplified using Pfu Turbo DNA polymerase (Stratagene) according to the supplier's instructions. .. Supernatants from 3 × 106 virus-infected cells were prepared on day two postinfection at a multiplicity of infection (MOI) of 1, and the virions were pelleted through 20% sucrose in TEN buffer (50 mM Tris-HCl, 1 mM EDTA, 150 mM NaCl [pH 7.4]) on a 60% sucrose cushion.

    Amplification:

    Article Title: Dissociation of Rabies Virus Matrix Protein Functions in Regulation of Viral RNA Synthesis and Virus Assembly
    Article Snippet: .. The first-strand cDNA was generated from 1 μg of RNA by using avian myeloblastosis virus reverse transcriptase (Stratagene). cDNA was PCR amplified using Pfu Turbo DNA polymerase (Stratagene) according to the supplier's instructions. .. Supernatants from 3 × 106 virus-infected cells were prepared on day two postinfection at a multiplicity of infection (MOI) of 1, and the virions were pelleted through 20% sucrose in TEN buffer (50 mM Tris-HCl, 1 mM EDTA, 150 mM NaCl [pH 7.4]) on a 60% sucrose cushion.

    Polymerase Chain Reaction:

    Article Title: Dissociation of Rabies Virus Matrix Protein Functions in Regulation of Viral RNA Synthesis and Virus Assembly
    Article Snippet: .. The first-strand cDNA was generated from 1 μg of RNA by using avian myeloblastosis virus reverse transcriptase (Stratagene). cDNA was PCR amplified using Pfu Turbo DNA polymerase (Stratagene) according to the supplier's instructions. .. Supernatants from 3 × 106 virus-infected cells were prepared on day two postinfection at a multiplicity of infection (MOI) of 1, and the virions were pelleted through 20% sucrose in TEN buffer (50 mM Tris-HCl, 1 mM EDTA, 150 mM NaCl [pH 7.4]) on a 60% sucrose cushion.

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  • 92
    Stratagene avian myeloblastosis virus reverse transcriptase
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase/product/Stratagene
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Stratagene amv reverse transcriptase
    Amv Reverse Transcriptase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amv reverse transcriptase/product/Stratagene
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    amv reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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