avian myeloblastosis virus reverse transcriptase  (Seikagaku)

 
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    Structured Review

    Seikagaku avian myeloblastosis virus reverse transcriptase
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Seikagaku, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase/product/Seikagaku
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars

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    Amplification:

    Article Title: Direct Quantification of Human Cytomegalovirus Immediate-Early and Late mRNA Levels in Blood of Lung Transplant Recipients by Competitive Nucleic Acid Sequence-Based Amplification
    Article Snippet: .. The amplification was initiated by an enzyme mix containing avian myeloblastosis virus reverse transcriptase (Seikagaku, Rockville, Md.), RNase H, and T7 RNA polymerase (Pharmacia, Uppsala, Sweden) ( ). .. Amplification products were detected by electrochemiluminescence system, using capture probes coupled to magnetic beads and wt- and Q-specific ruthenium-labeled oligonucleotide detection probes by the NASBA QR system (Organon Teknika, Boxtel, The Netherlands).

    Article Title: Embryonic neuronal markers in tuberous sclerosis: Single-cell molecular pathology
    Article Snippet: .. While IST had already been performed on sections, to ensure cDNA synthesis, the microelectrodes were filled with electrode buffer (10 mM Hepes buffer, pH 7.4/120 mM KCl/1 mM MgCl2 ), dNTPs (250 μM each), oligo-dT-T7 primer, and avian myeloblastosis virus reverse transcriptase, and cDNA synthesis was performed for 90 min at 40°C ( ). mRNA from sections and cells was amplified (aRNA) from double-stranded cDNA template with T7 RNA polymerase (Epicentre Technologies, Madison, WI) incorporating [32 P]CTP ( ). .. After initial amplification, a broad spectrum of high to low molecular weight mRNAs was detected from sections and individual cells (approximately 1–6 kbp); this spectrum was similar to that of frozen sections and whole live cells ( , ). aRNA served as a template for second-round cDNA synthesis, whose product then served as template for a second aRNA amplification incorporating [32 P]CTP.

    In Situ:

    Article Title: Early Progenitor Cell Marker Expression Distinguishes Type II from Type I Focal Cortical Dysplasias
    Article Snippet: .. Single cells were aspirated into a pipette tip, transferred to a microfuge tube containing in situ reverse transcription buffer and avian myeloblastosis virus reverse transcriptase and incubated at 4°C for 90 minutes to generate single stranded cDNA. .. Double stranded template cDNA was generated with T4 DNA polymerase I (Amersham, Piscataway, NJ) from cDNA.

    Fluorescence:

    Article Title: Real-Time Nucleic Acid Sequence-Based Amplification Assay for Detection of Hepatitis A Virus
    Article Snippet: .. After 5 min, 2 μl of an enzyme mixture containing 2.6 μg of bovine serum albumin (Promega Co.), 40 international units (IU) of T7 RNA polymerase (Novagen Inc., Madison, WI), 8 IU of avian myeloblastosis virus reverse transcriptase (Seikagaku, Ijamsville, Md.), 0.2 IU of RNase H (New England Biolabs, Beverly, MA), and 12.5 IU of RNasin (Amersham Biotech) were added to each well, followed by incubation at 40° ± 1°C for 150 min. Fluorescence intensity data were recorded every minute of the NASBA reaction. .. The threshold cycle of each amplification reaction was calculated based on the first cycle at which the fluorescence was 10-fold higher than the standard deviation of the mean baseline emission.

    Isolation:

    Article Title: Human Cytomegalovirus Virions Differentially Incorporate Viral and Host Cell RNA during the Assembly Process
    Article Snippet: .. NASBA reactions were carried out in a 20-μl reaction mixture containing 40 mM Tris (pH 8.5), 12 mM MgCl2 , 70 mM KCL, 15% (vol/vol) dimethyl sulfoxide; 5 mM dithiothreitol, each deoxynucleoside triphosphate at a concentration of 1 mM, 2 mM (each) ATP, CTP, and UTP, 1.5 mM GTP, 0.5 mM ITP, 2 μg of bovine serum albumin (Boehringer GmbH, Mannheim, Germany), 0.08 U of RNase H (Pharmacia), 32 U of T7 RNA polymerase (Pharmacia), 6.4 U of avian myeloblastosis virus reverse transcriptase (Seikagaku, Rockville, Md.), each primer at a concentration of 0.2 μM, and 5 μl of isolated nucleic acids. .. The NASBA reaction mixture without enzymes was incubated for 5 min at 65°C and was cooled to 41°C in 5 min, followed by addition of the enzymes.

    Transferring:

    Article Title: Early Progenitor Cell Marker Expression Distinguishes Type II from Type I Focal Cortical Dysplasias
    Article Snippet: .. Single cells were aspirated into a pipette tip, transferred to a microfuge tube containing in situ reverse transcription buffer and avian myeloblastosis virus reverse transcriptase and incubated at 4°C for 90 minutes to generate single stranded cDNA. .. Double stranded template cDNA was generated with T4 DNA polymerase I (Amersham, Piscataway, NJ) from cDNA.

    Incubation:

    Article Title: Early Progenitor Cell Marker Expression Distinguishes Type II from Type I Focal Cortical Dysplasias
    Article Snippet: .. Single cells were aspirated into a pipette tip, transferred to a microfuge tube containing in situ reverse transcription buffer and avian myeloblastosis virus reverse transcriptase and incubated at 4°C for 90 minutes to generate single stranded cDNA. .. Double stranded template cDNA was generated with T4 DNA polymerase I (Amersham, Piscataway, NJ) from cDNA.

    Article Title: Real-Time Nucleic Acid Sequence-Based Amplification Assay for Detection of Hepatitis A Virus
    Article Snippet: .. After 5 min, 2 μl of an enzyme mixture containing 2.6 μg of bovine serum albumin (Promega Co.), 40 international units (IU) of T7 RNA polymerase (Novagen Inc., Madison, WI), 8 IU of avian myeloblastosis virus reverse transcriptase (Seikagaku, Ijamsville, Md.), 0.2 IU of RNase H (New England Biolabs, Beverly, MA), and 12.5 IU of RNasin (Amersham Biotech) were added to each well, followed by incubation at 40° ± 1°C for 150 min. Fluorescence intensity data were recorded every minute of the NASBA reaction. .. The threshold cycle of each amplification reaction was calculated based on the first cycle at which the fluorescence was 10-fold higher than the standard deviation of the mean baseline emission.

    Article Title: Construction of an Infectious cDNA Clone of Aichi Virus (a New Member of the Family Picornaviridae) and Mutational Analysis of a Stem-Loop Structure at the 5? End of the Genome
    Article Snippet: .. To synthesize first-strand cDNA, 10 pmol of a primer and 50 ng of the genomic RNA were heated at 95°C for 2 min, chilled on ice, and then added to a reaction mixture (125 mM Tris-HCl [pH 8.3], 125 mM KCl, 25 mM magnesium acetate, 25 mM dithiothreitol, 250 μM of each deoxynucleoside triphosphate, and 10 U of avian myeloblastosis virus reverse transcriptase [Seikagaku Kogyo]), and then the mixture was incubated at 42°C for 50 min. A part of this RT reaction mixture was used for PCR. .. Five cDNA fragments corresponding to nt 1 to 1544, 1321 to 3803, 3524 to 5505, 5173 to 6784, and 6707 to 8251 (these nucleotide numbers refer to the sequence deposited previously in the DDBJ, EMBL, and GenBank databases) were amplified by PCR using primers sets T7AV1-1544M, 1321P-3803M, 3524P-5505M, 5173P-6784M, and 6707P-3′polyA, respectively.

    Article Title: Processing of the coronavirus MHV-JHM polymerase polyprotein: identification of precursors and proteolytic products spanning 400 kilodaltons of ORF1a.
    Article Snippet: .. The RNA was then incubated for 2 h at 42°C in a reaction mixture containing the following: 0.07 M ␤-mercaptoethanol, 0.1 g random hexamer oligonucleotide primers, 10 M each deoxyribonucleoside triphosphate, 85 mM Tris±HCl, pH 8.3, 34 mM KCl, 8.5 mM MgCl 2 , 40 U of RNAsin and 8 U of avian myeloblastosis virus reverse transcriptase (Seikagaku America, Inc., Rockville, MD). ..

    Concentration Assay:

    Article Title: Human Cytomegalovirus Virions Differentially Incorporate Viral and Host Cell RNA during the Assembly Process
    Article Snippet: .. NASBA reactions were carried out in a 20-μl reaction mixture containing 40 mM Tris (pH 8.5), 12 mM MgCl2 , 70 mM KCL, 15% (vol/vol) dimethyl sulfoxide; 5 mM dithiothreitol, each deoxynucleoside triphosphate at a concentration of 1 mM, 2 mM (each) ATP, CTP, and UTP, 1.5 mM GTP, 0.5 mM ITP, 2 μg of bovine serum albumin (Boehringer GmbH, Mannheim, Germany), 0.08 U of RNase H (Pharmacia), 32 U of T7 RNA polymerase (Pharmacia), 6.4 U of avian myeloblastosis virus reverse transcriptase (Seikagaku, Rockville, Md.), each primer at a concentration of 0.2 μM, and 5 μl of isolated nucleic acids. .. The NASBA reaction mixture without enzymes was incubated for 5 min at 65°C and was cooled to 41°C in 5 min, followed by addition of the enzymes.

    Random Hexamer Labeling:

    Article Title: Processing of the coronavirus MHV-JHM polymerase polyprotein: identification of precursors and proteolytic products spanning 400 kilodaltons of ORF1a.
    Article Snippet: .. The RNA was then incubated for 2 h at 42°C in a reaction mixture containing the following: 0.07 M ␤-mercaptoethanol, 0.1 g random hexamer oligonucleotide primers, 10 M each deoxyribonucleoside triphosphate, 85 mM Tris±HCl, pH 8.3, 34 mM KCl, 8.5 mM MgCl 2 , 40 U of RNAsin and 8 U of avian myeloblastosis virus reverse transcriptase (Seikagaku America, Inc., Rockville, MD). ..

    Polymerase Chain Reaction:

    Article Title: Construction of an Infectious cDNA Clone of Aichi Virus (a New Member of the Family Picornaviridae) and Mutational Analysis of a Stem-Loop Structure at the 5? End of the Genome
    Article Snippet: .. To synthesize first-strand cDNA, 10 pmol of a primer and 50 ng of the genomic RNA were heated at 95°C for 2 min, chilled on ice, and then added to a reaction mixture (125 mM Tris-HCl [pH 8.3], 125 mM KCl, 25 mM magnesium acetate, 25 mM dithiothreitol, 250 μM of each deoxynucleoside triphosphate, and 10 U of avian myeloblastosis virus reverse transcriptase [Seikagaku Kogyo]), and then the mixture was incubated at 42°C for 50 min. A part of this RT reaction mixture was used for PCR. .. Five cDNA fragments corresponding to nt 1 to 1544, 1321 to 3803, 3524 to 5505, 5173 to 6784, and 6707 to 8251 (these nucleotide numbers refer to the sequence deposited previously in the DDBJ, EMBL, and GenBank databases) were amplified by PCR using primers sets T7AV1-1544M, 1321P-3803M, 3524P-5505M, 5173P-6784M, and 6707P-3′polyA, respectively.

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  • About
  • News
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  • 92
    Seikagaku avian myeloblastosis virus reverse transcriptase
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Seikagaku, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase/product/Seikagaku
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    80
    Seikagaku amv seikagaku reverse transcriptase
    Amv Seikagaku Reverse Transcriptase, supplied by Seikagaku, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amv seikagaku reverse transcriptase/product/Seikagaku
    Average 80 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    amv seikagaku reverse transcriptase - by Bioz Stars, 2020-08
    80/100 stars
      Buy from Supplier

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