avian myeloblastosis virus reverse transcriptase  (PerkinElmer)

 
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    PerkinElmer avian myeloblastosis virus reverse transcriptase
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase/product/PerkinElmer
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars

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    Amplification:

    Article Title: Characterization of the RNA Components of a Putative Molecular Switch in the 3? Untranslated Region of the Murine Coronavirus Genome
    Article Snippet: .. Reverse transcription of RNA was performed with a random hexanucleotide primer (Boehringer Mannheim) and avian myeloblastosis virus reverse transcriptase (Life Sciences) under standard conditions ( ); the relevant region of the genome was then amplified by PCR under standard conditions using AmpliTaq polymerase (Perkin-Elmer). .. The resulting products were analyzed directly by agarose gel electrophoresis or purified with Quantum-prep columns (Bio-Rad) prior to automated sequencing.

    Article Title: Identification of Th2-type suppressor T cells among in vivo expanded ocular T cells in mice with experimental autoimmune uveoretinitis
    Article Snippet: .. Briefly, first strand cDNAs were synthesized in a 20-µl reaction mixture containing oligo(dT) primer by avian myeloblastosis virus reverse transcriptase from 1 µg of total RNA at 42°C for 60 h. For T cell recepter (TCR) BV family PCR analysis, cDNA were amplified by 30 cycles of denaturation (94°C for 1 minute), annealing (60°C for 1 minute), and extension (72°C for 1 minute), on a thermal cycler (Perkin-Elmer, Norwalk, CT) and specific 19 BV and BC primers from previously published data [ ]. .. For southern blot analysis, one-tenth of the PCR products was subjected to a 2% agarose gel electrophoresis and then was transferred to a nylon membrane and hybridized with digoxygenin (DIG)-labelled BC probe, visualized by the DIG Luminescent detection kit (Boehringer Mannheim).

    Synthesized:

    Article Title: Identification of Th2-type suppressor T cells among in vivo expanded ocular T cells in mice with experimental autoimmune uveoretinitis
    Article Snippet: .. Briefly, first strand cDNAs were synthesized in a 20-µl reaction mixture containing oligo(dT) primer by avian myeloblastosis virus reverse transcriptase from 1 µg of total RNA at 42°C for 60 h. For T cell recepter (TCR) BV family PCR analysis, cDNA were amplified by 30 cycles of denaturation (94°C for 1 minute), annealing (60°C for 1 minute), and extension (72°C for 1 minute), on a thermal cycler (Perkin-Elmer, Norwalk, CT) and specific 19 BV and BC primers from previously published data [ ]. .. For southern blot analysis, one-tenth of the PCR products was subjected to a 2% agarose gel electrophoresis and then was transferred to a nylon membrane and hybridized with digoxygenin (DIG)-labelled BC probe, visualized by the DIG Luminescent detection kit (Boehringer Mannheim).

    Labeling:

    Article Title: Genomic Approach to Identifying the Putative Target of and Mechanisms of Resistance to Mefloquine in Mycobacteria
    Article Snippet: .. The following master mix was made for cDNA labeling: 50 U avian myeloblastosis virus reverse transcriptase (Perkin-Elmer), 1× reverse transcriptase buffer, 333 μM dATP, 333 μM dGTP, 333 μM dTTP, 20 Ci [α-33 P]dCTP (2,000 to 3,000 Ci/mmol; Amersham Pharmacia Biotech), and RNase-free water. .. Labeling mix was added to each reaction mixture and incubated at 42°C for 2 h. Unincorporated-radiolabeled nucleotides were removed from labeled cDNA probe by purification over Spandex G-25 gel filtration columns according to the manufacturer's instructions (Sigma).

    Polymerase Chain Reaction:

    Article Title: Characterization of the RNA Components of a Putative Molecular Switch in the 3? Untranslated Region of the Murine Coronavirus Genome
    Article Snippet: .. Reverse transcription of RNA was performed with a random hexanucleotide primer (Boehringer Mannheim) and avian myeloblastosis virus reverse transcriptase (Life Sciences) under standard conditions ( ); the relevant region of the genome was then amplified by PCR under standard conditions using AmpliTaq polymerase (Perkin-Elmer). .. The resulting products were analyzed directly by agarose gel electrophoresis or purified with Quantum-prep columns (Bio-Rad) prior to automated sequencing.

    Article Title: Identification of Th2-type suppressor T cells among in vivo expanded ocular T cells in mice with experimental autoimmune uveoretinitis
    Article Snippet: .. Briefly, first strand cDNAs were synthesized in a 20-µl reaction mixture containing oligo(dT) primer by avian myeloblastosis virus reverse transcriptase from 1 µg of total RNA at 42°C for 60 h. For T cell recepter (TCR) BV family PCR analysis, cDNA were amplified by 30 cycles of denaturation (94°C for 1 minute), annealing (60°C for 1 minute), and extension (72°C for 1 minute), on a thermal cycler (Perkin-Elmer, Norwalk, CT) and specific 19 BV and BC primers from previously published data [ ]. .. For southern blot analysis, one-tenth of the PCR products was subjected to a 2% agarose gel electrophoresis and then was transferred to a nylon membrane and hybridized with digoxygenin (DIG)-labelled BC probe, visualized by the DIG Luminescent detection kit (Boehringer Mannheim).

    Cell Culture:

    Article Title: Iron regulatory protein 1 is not required for the modulation of ferritin and transferrin receptor expression by iron in a murine pro-B lymphocyte cell line
    Article Snippet: .. The following sources were used: [32 P]-labeled nucleotides, DuPont NEN; [35 S]Met/Cys, ICN; T7 RNA polymerase, New England Biolabs; RQ1 DNase, RNasin, and Taq polymerase, Promega; avian myeloblastosis virus reverse transcriptase, Perkin—Elmer; deoxynucleotide triphosphates, Boehringer Mannheim; 2-mercaptoethanol (2-ME), Fluka; cell culture products and TRIzol, GIBCO/BRL; bovine serums, HyClone; oligonucleotides, Research Genetics (Huntsville, AL); and Sequenase DNA sequencing system, Amersham. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Detection of human Coronavirus 229E in nasal specimens in large scale studies using an RT-PCR hybridization assay
    Article Snippet: .. Briefly, 30 μl of reaction mix contained 1X AMV/Tfl reaction buffer, 0.250 mM dNTP mix, 0.6 μM each of E7 and E9 primers, 0.2 mM of MgSO4 , 3 U of Avian Myeloblastosis Virus reverse transcriptase and 3 U of Tfl DNA polymerase and 15 μl of RNA extract RT-PCR was performed as follow in a 9600 Perkin Elmer thermocycler (PE Applied Biosystem, Courtaboeuf, France): 45 min at 48 °C and 2 min at 94 °C, 40 three-step cycles with 30 s denaturation at 94 °C, 1 min annealing at 55 °C, and 2 min extension at 68 °C and a final 7 min extension step at 68 °C. .. Each RT-PCR carried out included as many as 85 patient RNA extracts, 1 negative and 1 positive control (RNA extract from 10−4 dilution of HCoV-229E with a known titer of 3.16×105 TCID50 /ml).

    Article Title: Detection of human Coronavirus 229E in nasal specimens in large scale studies using an RT-PCR hybridization assay.
    Article Snippet: .. Briefly, 30 ml of reaction mix contained 1X AMV/Tfl reaction buffer, 0.250 mM dNTP mix, 0.6 mM each of E7 and E9 primers, 0.2 mM of MgSO 4 , 3 U of Avian Myeloblastosis Virus reverse transcriptase and 3 U of Tfl DNA polymerase and 15 ml of RNA extract RT-PCR was performed as follow in a 9600 Perkin Elmer thermocycler (PE Applied Biosystem, Courtaboeuf, France): 45 min at 48 8C and 2 min at 94 8C, 40 three-step cycles with 30 s denaturation at 94 8C, 1 min annealing at 55 8C, and 2 min extension at 68 8C and a final 7 min extension step at 68 8C. .. One step RT-PCR Each RT-PCR carried out included as many as 85 patient RNA extracts, 1 negative and 1 positive control (RNA extract from 10 24 dilution of HCoV-229E with a known titer of 3.16 £ 10 5 TCID 50 /ml).

    DNA Sequencing:

    Article Title: Iron regulatory protein 1 is not required for the modulation of ferritin and transferrin receptor expression by iron in a murine pro-B lymphocyte cell line
    Article Snippet: .. The following sources were used: [32 P]-labeled nucleotides, DuPont NEN; [35 S]Met/Cys, ICN; T7 RNA polymerase, New England Biolabs; RQ1 DNase, RNasin, and Taq polymerase, Promega; avian myeloblastosis virus reverse transcriptase, Perkin—Elmer; deoxynucleotide triphosphates, Boehringer Mannheim; 2-mercaptoethanol (2-ME), Fluka; cell culture products and TRIzol, GIBCO/BRL; bovine serums, HyClone; oligonucleotides, Research Genetics (Huntsville, AL); and Sequenase DNA sequencing system, Amersham. ..

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  • 92
    PerkinElmer avian myeloblastosis virus reverse transcriptase
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase/product/PerkinElmer
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    88
    PerkinElmer avian myeloblastosis virus reverse transcriptase primer extension system
    Avian Myeloblastosis Virus Reverse Transcriptase Primer Extension System, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase primer extension system/product/PerkinElmer
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transcriptase primer extension system - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    80
    PerkinElmer amv reverse transcriptase first strand cdna synthesis kit
    Amv Reverse Transcriptase First Strand Cdna Synthesis Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amv reverse transcriptase first strand cdna synthesis kit/product/PerkinElmer
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amv reverse transcriptase first strand cdna synthesis kit - by Bioz Stars, 2020-08
    80/100 stars
      Buy from Supplier

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