avian myeloblastosis virus reverse transcriptase  (GE Healthcare)

 
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    Structured Review

    GE Healthcare avian myeloblastosis virus reverse transcriptase
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase/product/GE Healthcare
    Average 93 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transcriptase - by Bioz Stars, 2020-08
    93/100 stars

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    Amplification:

    Article Title: A modular, bifunctional RNA that integrates itself into a target RNA
    Article Snippet: .. During some rounds of evolution, the molecules were reverse-transcribed with avian myeloblastosis virus reverse transcriptase (Amersham Pharmacia), and full-length cDNAs were purified by PAGE and subsequently amplified by PCR. .. A nested PCR was performed to restore the 3′ terminus by using the same forward primer and a reverse primer complementary to the last 20 nt of the group I ribozyme.

    Synthesized:

    Article Title: Preventive Effect of Polysaccharide of Larimichthys crocea Swim Bladder on Reserpine Induced Gastric Ulcer in ICR Mice
    Article Snippet: .. The RNA was digested with RNase-free DNase (Roche, Basel, Switzerland) for 15 min at 37℃ and purified using an RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. cDNA was synthesized from 2 µg of total RNA by incubation at 37℃ for l h with avian myeloblastosis virus reverse transcriptase (GE Healthcare, Little Chalfont, United Kingdom) with random hexanucleotides according to the manufacturer's instruction. .. Sequences of primers used to specifically amplify the genes of interest were as follows: 5'-CAC TTA TGG ACA ACT ATG AGG TCT CTG G-3' (forward) and 5'-CTG TCT TGT GGA CAA CGC AGT GGA ATT TTA GG-3' (reverse) for NF-κB; 5'-GCT GAA GAA GGA GCG GCT ACT-3' (forward) and 5'-TCG TAC TCC TCG TCT TTC ATG GA-3' (reverse) for IκB-α; 5'-AGA GAG ATC GGG TTC ACA-3' (forward) and 5'-CAC AGA ACT GAG GGT ACA-3' (reverse) for iNOS; 5'-TTA AAA TGA GAT TGT CCG AA-3' (forward) and 5'-AGA TCA CCT CTG CCT GAG TA-3' (reverse) for COX-2.

    Article Title: Increased Preventive Effect on Colon Carcinogenesis by Use of Resistant Starch (RS3) as the Carrier for Polysaccharide of Larimichthys Crocea Swimming Bladder
    Article Snippet: .. The RNA was digested with RNase-free DNase (Roche, Basel, Switzerland) for 15 min at 37 °C and purified using an RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. cDNA was synthesized from 2 μg of total RNA by incubation at 37 °C for l h with avian myeloblastosis virus reverse transcriptase (GE Healthcare, Little Chalfont, UK) with random hexanucleotides according to the manufacturer’s instruction. ..

    Article Title: In vitro and in vivo anti-cancer activities of Kuding tea (Ilex kudingcha C.J. Tseng) against oral cancer
    Article Snippet: .. The RNA was digested with RNase (Roche, Basel, Switzerland) for 15 min at 37ºC and purified using an RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. cDNA was synthesized from 2 μg total RNA by incubating at 37ºC for l h with avian myeloblastosis virus reverse transcriptase (GE Healthcare, Little Chalfont, UK) with random hexanucleotides according to the manufacturer’s instructions. ..

    Incubation:

    Article Title: Preventive Effect of Polysaccharide of Larimichthys crocea Swim Bladder on Reserpine Induced Gastric Ulcer in ICR Mice
    Article Snippet: .. The RNA was digested with RNase-free DNase (Roche, Basel, Switzerland) for 15 min at 37℃ and purified using an RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. cDNA was synthesized from 2 µg of total RNA by incubation at 37℃ for l h with avian myeloblastosis virus reverse transcriptase (GE Healthcare, Little Chalfont, United Kingdom) with random hexanucleotides according to the manufacturer's instruction. .. Sequences of primers used to specifically amplify the genes of interest were as follows: 5'-CAC TTA TGG ACA ACT ATG AGG TCT CTG G-3' (forward) and 5'-CTG TCT TGT GGA CAA CGC AGT GGA ATT TTA GG-3' (reverse) for NF-κB; 5'-GCT GAA GAA GGA GCG GCT ACT-3' (forward) and 5'-TCG TAC TCC TCG TCT TTC ATG GA-3' (reverse) for IκB-α; 5'-AGA GAG ATC GGG TTC ACA-3' (forward) and 5'-CAC AGA ACT GAG GGT ACA-3' (reverse) for iNOS; 5'-TTA AAA TGA GAT TGT CCG AA-3' (forward) and 5'-AGA TCA CCT CTG CCT GAG TA-3' (reverse) for COX-2.

    Article Title: Increased Preventive Effect on Colon Carcinogenesis by Use of Resistant Starch (RS3) as the Carrier for Polysaccharide of Larimichthys Crocea Swimming Bladder
    Article Snippet: .. The RNA was digested with RNase-free DNase (Roche, Basel, Switzerland) for 15 min at 37 °C and purified using an RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. cDNA was synthesized from 2 μg of total RNA by incubation at 37 °C for l h with avian myeloblastosis virus reverse transcriptase (GE Healthcare, Little Chalfont, UK) with random hexanucleotides according to the manufacturer’s instruction. ..

    Article Title: Preventive effect of polysaccharides from the large yellow croaker swim bladder on HCl/ethanol induced gastric injury in mice
    Article Snippet: .. Total RNA (2 μg) was incubated at 37°C for l h with avian myeloblastosis virus reverse transcriptase (GE Healthcare, Little Chalfont, United Kingdom) with random hexanucleotides to form cDNA, in accordance with the manufacturer’s instructions. .. The following primers were used to specifically amplify the genes of interest: inducible nitric oxide synthase (iNOS) forward, 5′-AGA GAG ATC GGG TTC ACA-3′ and reverse, 5′-CAC AGA ACT GAG GGT ACA-3′; cyclooxygenase-2 (COX-2) forward, 5′-TTA AAA TGA GAT TGT CCG AA-3′ and reverse, 5′-AGA TCA CCT CTG CCT GAG TA-3′; tumor necrosis factor-α (TNF-α) forward, 5′-GAC CCT CAG ACT CAG ATC ATC CTT CT-3′ and reverse, 5′-ACG CTG GCT CAG CCA CTC-3′; and IL-1β forward, 5′-CTC CAT GAG CTT TGT ACA AGG-3′ and reverse, 5′-TGC TGA TGT ACC AGT TGG GG-3′.

    Purification:

    Article Title: A modular, bifunctional RNA that integrates itself into a target RNA
    Article Snippet: .. During some rounds of evolution, the molecules were reverse-transcribed with avian myeloblastosis virus reverse transcriptase (Amersham Pharmacia), and full-length cDNAs were purified by PAGE and subsequently amplified by PCR. .. A nested PCR was performed to restore the 3′ terminus by using the same forward primer and a reverse primer complementary to the last 20 nt of the group I ribozyme.

    Article Title: Preventive Effect of Polysaccharide of Larimichthys crocea Swim Bladder on Reserpine Induced Gastric Ulcer in ICR Mice
    Article Snippet: .. The RNA was digested with RNase-free DNase (Roche, Basel, Switzerland) for 15 min at 37℃ and purified using an RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. cDNA was synthesized from 2 µg of total RNA by incubation at 37℃ for l h with avian myeloblastosis virus reverse transcriptase (GE Healthcare, Little Chalfont, United Kingdom) with random hexanucleotides according to the manufacturer's instruction. .. Sequences of primers used to specifically amplify the genes of interest were as follows: 5'-CAC TTA TGG ACA ACT ATG AGG TCT CTG G-3' (forward) and 5'-CTG TCT TGT GGA CAA CGC AGT GGA ATT TTA GG-3' (reverse) for NF-κB; 5'-GCT GAA GAA GGA GCG GCT ACT-3' (forward) and 5'-TCG TAC TCC TCG TCT TTC ATG GA-3' (reverse) for IκB-α; 5'-AGA GAG ATC GGG TTC ACA-3' (forward) and 5'-CAC AGA ACT GAG GGT ACA-3' (reverse) for iNOS; 5'-TTA AAA TGA GAT TGT CCG AA-3' (forward) and 5'-AGA TCA CCT CTG CCT GAG TA-3' (reverse) for COX-2.

    Article Title: Increased Preventive Effect on Colon Carcinogenesis by Use of Resistant Starch (RS3) as the Carrier for Polysaccharide of Larimichthys Crocea Swimming Bladder
    Article Snippet: .. The RNA was digested with RNase-free DNase (Roche, Basel, Switzerland) for 15 min at 37 °C and purified using an RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. cDNA was synthesized from 2 μg of total RNA by incubation at 37 °C for l h with avian myeloblastosis virus reverse transcriptase (GE Healthcare, Little Chalfont, UK) with random hexanucleotides according to the manufacturer’s instruction. ..

    Article Title: In vitro and in vivo anti-cancer activities of Kuding tea (Ilex kudingcha C.J. Tseng) against oral cancer
    Article Snippet: .. The RNA was digested with RNase (Roche, Basel, Switzerland) for 15 min at 37ºC and purified using an RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. cDNA was synthesized from 2 μg total RNA by incubating at 37ºC for l h with avian myeloblastosis virus reverse transcriptase (GE Healthcare, Little Chalfont, UK) with random hexanucleotides according to the manufacturer’s instructions. ..

    Polymerase Chain Reaction:

    Article Title: A modular, bifunctional RNA that integrates itself into a target RNA
    Article Snippet: .. During some rounds of evolution, the molecules were reverse-transcribed with avian myeloblastosis virus reverse transcriptase (Amersham Pharmacia), and full-length cDNAs were purified by PAGE and subsequently amplified by PCR. .. A nested PCR was performed to restore the 3′ terminus by using the same forward primer and a reverse primer complementary to the last 20 nt of the group I ribozyme.

    Random Hexamer Labeling:

    Article Title: Dissection of 16S rRNA Methyltransferase (KsgA) Function in Escherichia coli ▿
    Article Snippet: .. One hundred units of avian myeloblastosis virus reverse transcriptase was used to label mRNAs in 80 μg of total RNAs with 0.1 mM Cy3-dUTP and Cy5-dUTP (Amersham), using 300 pmol pd(N)6 random hexamer (Amersham) as the primer. .. The E. coli gene array IntelliGene E. coli CHIP, version 2 (catalog no. X003), was obtained from Takara-Bio and contains approximately 4,150 immobilized DNA fragments.

    Polyacrylamide Gel Electrophoresis:

    Article Title: A modular, bifunctional RNA that integrates itself into a target RNA
    Article Snippet: .. During some rounds of evolution, the molecules were reverse-transcribed with avian myeloblastosis virus reverse transcriptase (Amersham Pharmacia), and full-length cDNAs were purified by PAGE and subsequently amplified by PCR. .. A nested PCR was performed to restore the 3′ terminus by using the same forward primer and a reverse primer complementary to the last 20 nt of the group I ribozyme.

    Sequencing:

    Article Title: Translation Limits Synthesis of an Assembly-Initiating Coat Protein of Filamentous Phage IKe
    Article Snippet: .. Preincubations were for 10 min at 37 or 42°C prior to the addition of 4 U of avian myeloblastosis virus reverse transcriptase (Amersham Corp.), and incubations were for 15 min. Sequencing reaction mixtures omitted tRNA and 30S ribosomes and contained a ddNTP at 200 μM. ..

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  • 92
    GE Healthcare avian myeloblastosis virus amv reverse transcriptase
    Avian Myeloblastosis Virus Amv Reverse Transcriptase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus amv reverse transcriptase/product/GE Healthcare
    Average 92 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus amv reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
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