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Avanti Polar avanti mini extruder
Avanti Mini Extruder, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/avanti mini extruder/product/Avanti Polar
Average 99 stars, based on 30 article reviews
Price from $9.99 to $1999.99
avanti mini extruder - by Bioz Stars, 2020-02
99/100 stars

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Related Articles

Clone Assay:

Article Title: Entry of Bluetongue Virus Capsid Requires the Late Endosome-specific Lipid Lysobisphosphatidic Acid *
Article Snippet: Lipids phosphatidylcholine (PC), phosphatidylethanolamine, sphingomyelin, phosphatidylinositol, phosphatidylserine (PS), LBPA, and Avanti® Mini-Extruder were purchased from Avanti Polar lipids. .. The pTriExHMBP-TEV-VP5 transfer vector was constructed by cloning the coding region of BTV-1 VP5 into a BamHI site of a premade (in house) empty pTriExHMBP-TEV vector in which cloning into a BamHI site places the gene of interest in-frame with and upstream of the HMBP-TEV cassette for which the GS of the ENLYFQGS TEV site represents the in-frame translation of the BamHI restriction site.

Filtration:

Article Title: Hydrophilic Linear Peptide with Histidine and Lysine Residues as a Key Factor Affecting Antifungal Activity
Article Snippet: The suspension was freeze-thawed in liquid nitrogen for nine cycles to generate large unilamellar vesicles (LUVs) and extruded 30 times through polycarbonate filters (two stacked 0.2-μm pore-size filters) using an Avanti Mini-Extruder (Avanti Polar Lipids Inc., Alabaster, AL, USA). .. Calcein-entrapped vesicles were separated from free calcein by gel filtration chromatography on a Sephadex G-50 column.

Stable Transfection:

Article Title: Plasma Exosomes Contribute to Microvascular Damage in Diabetic Retinopathy by Activating the Classical Complement Pathway
Article Snippet: Quantification of Exosomes in Control and Diabetic Plasma Quantification of circulating exosomes is challenging because of the lack of stably expressed exosomal markers. .. Microvesicles of known size, composition, and concentration were extruded using an Avanti Mini-Extruder (Avanti Polar Lipids, Inc.) with 100-nm pore filters; these microvesicles were used to create calibration curves (particles per milliliter).

Neutralization:

Article Title: In Vitro Reconstitution of Functional Type III Protein Export and Insights into Flagellar Assembly
Article Snippet: Three hundred microliters of the frozen stock solution of IMVs was thawed at room temperature and homogenized 10 times through an 0.8-µm polycarbonate membrane with the Avanti Mini-Extruder (Avanti Polar Lipids). .. The ATP solution was prepared by dissolving ATP (dipotassium salt) in Tris-HCl solution (final concentration of 20 mM) followed by neutralization with KOH.

Blocking Assay:

Article Title: Entry of Bluetongue Virus Capsid Requires the Late Endosome-specific Lipid Lysobisphosphatidic Acid *
Article Snippet: For antibody uptake blocking assays, a purified version of the monoclonal anti-LBPA antibody (P-SLBPA) (Echlelon Biosciences Inc.) and a monoclonal anti-FLAG® M2 mouse antibody (Sigma-Aldrich) were utilized. .. Lipids phosphatidylcholine (PC), phosphatidylethanolamine, sphingomyelin, phosphatidylinositol, phosphatidylserine (PS), LBPA, and Avanti® Mini-Extruder were purchased from Avanti Polar lipids.

Enzyme-linked Immunosorbent Assay:

Article Title: Entry of Bluetongue Virus Capsid Requires the Late Endosome-specific Lipid Lysobisphosphatidic Acid *
Article Snippet: Paraformaldehyde, BSA, Triton X-100, alkaline phosphatase liquid ELISA substrate, DMSO, and U18886A inhibitors were purchased from Sigma-Aldrich. .. Lipids phosphatidylcholine (PC), phosphatidylethanolamine, sphingomyelin, phosphatidylinositol, phosphatidylserine (PS), LBPA, and Avanti® Mini-Extruder were purchased from Avanti Polar lipids.

Incubation:

Article Title: Nuclear accumulation of plasmid DNA can be enhanced by non-selective gating of the nuclear pore
Article Snippet: Thereafter, the formed liposomes were extruded 11 times through two stacked 100 nm polycarbonate membrane filters (Whatman, Brentfort, UK) at room temperature using an Avanti Mini-Extruder (Avanti Polar Lipids). .. The extruded liposomes were subsequently mixed with pDNA in a ±charge ratio of 4 and incubated at room temperature for 30 min prior to use.

Article Title: Hydrophilic Linear Peptide with Histidine and Lysine Residues as a Key Factor Affecting Antifungal Activity
Article Snippet: The dried lipids were prehydrated with dye buffer solution (80 mM calcein, 10 mM MES, 50 mM NaCl, pH 6.2), vortexed for 1 min, and incubated for 30 min at 50 °C. .. The suspension was freeze-thawed in liquid nitrogen for nine cycles to generate large unilamellar vesicles (LUVs) and extruded 30 times through polycarbonate filters (two stacked 0.2-μm pore-size filters) using an Avanti Mini-Extruder (Avanti Polar Lipids Inc., Alabaster, AL, USA).

Article Title: Yeast Lipids Can Phase-separate into Micrometer-scale Membrane Domains *
Article Snippet: The samples were incubated at 68 °C, 1000 rpm for 30 min followed by five freeze-thaw cycles (freeze in liquid nitrogen, thaw at 25 °C and 1000 rpm). .. Liposomes were formed by extrusion at 68 °C using gas-tight syringes (Hamilton), 100-nm Whatman® Nuclepore track-etch membranes (Schleicher and Schuell), Whatman drain discs, and the Avanti mini-extruder (Avanti Polar Lipids).

Article Title: Effect of repetitive lysine-tryptophan motifs on the bactericidal activity of antimicrobial peptides
Article Snippet: The lipid suspensions were then sonicated at 40° C until clear using a bath-type ultrasonicator and extruded 14 times using an Avanti Mini-Extruder (Avanti Polar Lipids Inc., Alabaster, AL, USA) with 0.05 μm polycarbonate filters. .. LUVs were prepared through nine freeze–thaw cycles in liquid nitrogen, followed by incubation in a water bath at 50° C. The suspensions were then extruded 14 times through 0.2-μm polycarbonate membranes, and lipid concentrations were determined using standard phosphate assays (Stewart ).

Modification:

Article Title: Dimyristoylphosphotidylcholine induces conformational changes in apoB that lowers lipoprotein(a)
Article Snippet: Small unilamellar vesicles of DMPC (Avanti Polar Lipid, Alabaster, AL) were prepared using the method described by New ( ) with slight modification. .. The DMPC vesicles were extruded through a polycarbonate membrane with a pore size of 30 nm using the Avanti Mini extruder (Avanti Polar Lipid).

High Performance Liquid Chromatography:

Article Title: Effects of Ethanol on Conformational Changes of Akt Studied by Chemical Cross-Linking, Mass Spectrometry, and 18O Labeling
Article Snippet: The lipid suspension was extruded 10 times through a 0.1 μ m polycarbonate membrane (Corning, Inc.) using an Avanti mini-extruder (Avanti Polar Lipids). .. An aliquot of the sample was analyzed by high performance liquid chromatography–mass spectrometry (HPLC–MS) to verify the final concentrations of lipid components.

Colorimetric Assay:

Article Title: Mutual effects of MinD-membrane interaction: II. Domain structure of the membrane enhances MinD binding
Article Snippet: LUVs were prepared by extruding the suspension through a polycarbonate membrane filter (0.1 μm pore size, 19 mm diameter) 11 times using Avanti Mini-Extruder (Avanti Polar Lipids, Inc). .. Phospholipids concentration was determined by phosphomolybdate colorimetric assay [ ].

Transfection:

Article Title: Inhibition of NADPH oxidase by glucosylceramide confers chemoresistance
Article Snippet: Paragraph title: Liposome preparation and cellular transfection. ... The lipid vesicle-containing solution was quickly extruded at 60°C by passing the solution 10 times through 100 nm polycarbonate filters in an Avanti Mini-Extruder (Avanti Polar Lipids, Alabaster, AL).

Chromatography:

Article Title: Hydrophilic Linear Peptide with Histidine and Lysine Residues as a Key Factor Affecting Antifungal Activity
Article Snippet: The suspension was freeze-thawed in liquid nitrogen for nine cycles to generate large unilamellar vesicles (LUVs) and extruded 30 times through polycarbonate filters (two stacked 0.2-μm pore-size filters) using an Avanti Mini-Extruder (Avanti Polar Lipids Inc., Alabaster, AL, USA). .. Calcein-entrapped vesicles were separated from free calcein by gel filtration chromatography on a Sephadex G-50 column.

Article Title: Effects of Ethanol on Conformational Changes of Akt Studied by Chemical Cross-Linking, Mass Spectrometry, and 18O Labeling
Article Snippet: The lipid suspension was extruded 10 times through a 0.1 μ m polycarbonate membrane (Corning, Inc.) using an Avanti mini-extruder (Avanti Polar Lipids). .. An aliquot of the sample was analyzed by high performance liquid chromatography–mass spectrometry (HPLC–MS) to verify the final concentrations of lipid components.

Sublimation:

Article Title: Mutual effects of MinD-membrane interaction: II. Domain structure of the membrane enhances MinD binding
Article Snippet: Dried phospholipids were hydrated in a 25 mM Tris-HCl, pH 7.5, 50 mM KCl buffer above the phase transition temperature for 1 h and then were vigorously vortexed. .. LUVs were prepared by extruding the suspension through a polycarbonate membrane filter (0.1 μm pore size, 19 mm diameter) 11 times using Avanti Mini-Extruder (Avanti Polar Lipids, Inc).

Article Title: First Experimental Evidence of Dopamine Interactions with Negatively Charged Model Biomembranes
Article Snippet: Subsequently, the thin film of lipid was suspended in an appropriate amount of warm 50 mM phosphate buffer (pH 7.2) to give a final lipid concentration 20 mM (by intensive vortexing at temperature above the lipid phase transition). .. In order to obtain LUVs from MLVs, suspensions of MLVs were extruded at least 21 times through a Nucleopore polycarbonate membrane (Whatman, pore diameter of 100 nm) in Avanti Mini-Extruder (Avanti Polar Lipids Inc.).

Article Title: Dimyristoylphosphotidylcholine induces conformational changes in apoB that lowers lipoprotein(a)
Article Snippet: The temperature was maintained at 30°C, above the phase transition temperature of DMPC (∼24°C). .. The DMPC vesicles were extruded through a polycarbonate membrane with a pore size of 30 nm using the Avanti Mini extruder (Avanti Polar Lipid).

other:

Article Title: Protein Corona Fingerprints of Liposomes: New Opportunities for Targeted Drug Delivery and Early Detection in Pancreatic Cancer
Article Snippet: The obtained solutions were extruded 20 times through a 0.1-μm polycarbonate carbonate filter with the Avanti Mini-Extruder (Avanti Polar Lipids, Alabaster, AL, USA) and stored at 4 °C until use.

Serial Time-encoded Amplified Microscopy:

Article Title: Effects of Ethanol on Conformational Changes of Akt Studied by Chemical Cross-Linking, Mass Spectrometry, and 18O Labeling
Article Snippet: The mixture was dried under an N2 steam, redissolved in 2 mL of cyclohexane containing 75 μ M BHT, and lyophilized for 2 h under vacuum. .. The lipid suspension was extruded 10 times through a 0.1 μ m polycarbonate membrane (Corning, Inc.) using an Avanti mini-extruder (Avanti Polar Lipids).

Phospholipid Assay:

Article Title: Dimyristoylphosphotidylcholine induces conformational changes in apoB that lowers lipoprotein(a)
Article Snippet: The DMPC vesicles were extruded through a polycarbonate membrane with a pore size of 30 nm using the Avanti Mini extruder (Avanti Polar Lipid). .. The concentration of phospholipid was determined using the Wako phospholipid assay kit.

Sonication:

Article Title: Dimyristoylphosphotidylcholine induces conformational changes in apoB that lowers lipoprotein(a)
Article Snippet: The DMPC was suspended in PBS, pH 7.4, and sonicated using a microtip 3 mm probe (Sonics VibraCell, Newtown, CT) at an amplitude of 20 MHz for 20 × 30 s with 30-s breaks in between. .. The DMPC vesicles were extruded through a polycarbonate membrane with a pore size of 30 nm using the Avanti Mini extruder (Avanti Polar Lipid).

Article Title: Ceramide-tamoxifen regimen targets bioenergetic elements in acute myelogenous leukemia 1
Article Snippet: Solutions were sealed, heated at 55°C for 60 min, vortex mixed, and sonicated for 5 min until light no longer diffracted through the suspension. .. The solution was quickly extruded at 55°C by passage through 100 nm polycarbonate filters in an Avanti Mini-Extruder (Avanti Polar Lipids).

Article Title: Whole Cell Screen for Inhibitors of pH Homeostasis in Mycobacterium tuberculosis
Article Snippet: .. The suspension was sonicated at low power for 1 min, subjected to five freeze-thaw cycles and extruded 21 times at room temperature using an Avanti mini-extruder with a 0.1 µm polycarbonate membrane filter. .. Unencapsulated ANTS was removed using a PD-10 desalting column (GE Healthcare, Piscataway, NJ), and the vesicle stock solution (∼5 mM lipid) was stored in the dark at 12.5°C for a maximum of seven days.

Article Title: Effect of repetitive lysine-tryptophan motifs on the bactericidal activity of antimicrobial peptides
Article Snippet: .. The lipid suspensions were then sonicated at 40° C until clear using a bath-type ultrasonicator and extruded 14 times using an Avanti Mini-Extruder (Avanti Polar Lipids Inc., Alabaster, AL, USA) with 0.05 μm polycarbonate filters. ..

Fluorescence:

Article Title: Hydrophilic Linear Peptide with Histidine and Lysine Residues as a Key Factor Affecting Antifungal Activity
Article Snippet: The suspension was freeze-thawed in liquid nitrogen for nine cycles to generate large unilamellar vesicles (LUVs) and extruded 30 times through polycarbonate filters (two stacked 0.2-μm pore-size filters) using an Avanti Mini-Extruder (Avanti Polar Lipids Inc., Alabaster, AL, USA). .. The fluorescence of the released calcein was assessed with a microtiter SpectraMax M5 reader at an excitation wavelength of 480 nm and an emission wavelength of 520 nm.

Electron Microscopy:

Article Title: Plasma Exosomes Contribute to Microvascular Damage in Diabetic Retinopathy by Activating the Classical Complement Pathway
Article Snippet: Microvesicles of known size, composition, and concentration were extruded using an Avanti Mini-Extruder (Avanti Polar Lipids, Inc.) with 100-nm pore filters; these microvesicles were used to create calibration curves (particles per milliliter). .. Vesicles extruded through a 100-nm pore filter had a diameter similar to that of mouse plasma exosomes ( ); this similarity was further confirmed by electron microscopy ( ).

Isolation:

Article Title: Plasma Exosomes Contribute to Microvascular Damage in Diabetic Retinopathy by Activating the Classical Complement Pathway
Article Snippet: Microvesicles of known size, composition, and concentration were extruded using an Avanti Mini-Extruder (Avanti Polar Lipids, Inc.) with 100-nm pore filters; these microvesicles were used to create calibration curves (particles per milliliter). .. DLS readings of extruded vesicles were compared with DLS readings of isolated mouse plasma exosomes ( ).

Microscopy:

Article Title: Yeast Lipids Can Phase-separate into Micrometer-scale Membrane Domains *
Article Snippet: Paragraph title: C-laurdan Spectroscopy and Microscopy ... Liposomes were formed by extrusion at 68 °C using gas-tight syringes (Hamilton), 100-nm Whatman® Nuclepore track-etch membranes (Schleicher and Schuell), Whatman drain discs, and the Avanti mini-extruder (Avanti Polar Lipids).

Purification:

Article Title: In Vitro Reconstitution of Functional Type III Protein Export and Insights into Flagellar Assembly
Article Snippet: Three hundred microliters of the frozen stock solution of IMVs was thawed at room temperature and homogenized 10 times through an 0.8-µm polycarbonate membrane with the Avanti Mini-Extruder (Avanti Polar Lipids). .. The transport assay solution was prepared by mixing 100 µl of the IMV solution with 375 µl of external buffer containing Tris-HCl, pH 7.5, KCl, MgCl2 , dithiothreitol (DTT), and purified substrate proteins, and protein export was initiated by adding 25 µl of 0.1 M ATP solution.

Article Title: Entry of Bluetongue Virus Capsid Requires the Late Endosome-specific Lipid Lysobisphosphatidic Acid *
Article Snippet: For antibody uptake blocking assays, a purified version of the monoclonal anti-LBPA antibody (P-SLBPA) (Echlelon Biosciences Inc.) and a monoclonal anti-FLAG® M2 mouse antibody (Sigma-Aldrich) were utilized. .. Lipids phosphatidylcholine (PC), phosphatidylethanolamine, sphingomyelin, phosphatidylinositol, phosphatidylserine (PS), LBPA, and Avanti® Mini-Extruder were purchased from Avanti Polar lipids.

Construct:

Article Title: Entry of Bluetongue Virus Capsid Requires the Late Endosome-specific Lipid Lysobisphosphatidic Acid *
Article Snippet: Lipids phosphatidylcholine (PC), phosphatidylethanolamine, sphingomyelin, phosphatidylinositol, phosphatidylserine (PS), LBPA, and Avanti® Mini-Extruder were purchased from Avanti Polar lipids. .. The pTriExHMBP-TEV-VP5 transfer vector was constructed by cloning the coding region of BTV-1 VP5 into a BamHI site of a premade (in house) empty pTriExHMBP-TEV vector in which cloning into a BamHI site places the gene of interest in-frame with and upstream of the HMBP-TEV cassette for which the GS of the ENLYFQGS TEV site represents the in-frame translation of the BamHI restriction site.

Spectroscopy:

Article Title: Yeast Lipids Can Phase-separate into Micrometer-scale Membrane Domains *
Article Snippet: Paragraph title: C-laurdan Spectroscopy and Microscopy ... Liposomes were formed by extrusion at 68 °C using gas-tight syringes (Hamilton), 100-nm Whatman® Nuclepore track-etch membranes (Schleicher and Schuell), Whatman drain discs, and the Avanti mini-extruder (Avanti Polar Lipids).

Plasmid Preparation:

Article Title: Inhibition of NADPH oxidase by glucosylceramide confers chemoresistance
Article Snippet: The lipid vesicle-containing solution was quickly extruded at 60°C by passing the solution 10 times through 100 nm polycarbonate filters in an Avanti Mini-Extruder (Avanti Polar Lipids, Alabaster, AL). .. To prepare siRNA- or plasmid-loaded cationic nanoliposomes, siRNA or plasmid DNA, was aliquoted into 0.9% NaCl and nanoliposomes were added in a 10:1 weight ratio to nucleic acid.

Article Title: Entry of Bluetongue Virus Capsid Requires the Late Endosome-specific Lipid Lysobisphosphatidic Acid *
Article Snippet: Lipids phosphatidylcholine (PC), phosphatidylethanolamine, sphingomyelin, phosphatidylinositol, phosphatidylserine (PS), LBPA, and Avanti® Mini-Extruder were purchased from Avanti Polar lipids. .. The pTriExHMBP-TEV-VP5 transfer vector was constructed by cloning the coding region of BTV-1 VP5 into a BamHI site of a premade (in house) empty pTriExHMBP-TEV vector in which cloning into a BamHI site places the gene of interest in-frame with and upstream of the HMBP-TEV cassette for which the GS of the ENLYFQGS TEV site represents the in-frame translation of the BamHI restriction site.

Transport Assay:

Article Title: In Vitro Reconstitution of Functional Type III Protein Export and Insights into Flagellar Assembly
Article Snippet: Paragraph title: Transport assay. ... Three hundred microliters of the frozen stock solution of IMVs was thawed at room temperature and homogenized 10 times through an 0.8-µm polycarbonate membrane with the Avanti Mini-Extruder (Avanti Polar Lipids).

Evaporation:

Article Title: Nuclear accumulation of plasmid DNA can be enhanced by non-selective gating of the nuclear pore
Article Snippet: The chloroform was subsequently removed by rotary evaporation at 37°C followed by flushing the obtained lipid film with nitrogen during 30 min at room temperature. .. Thereafter, the formed liposomes were extruded 11 times through two stacked 100 nm polycarbonate membrane filters (Whatman, Brentfort, UK) at room temperature using an Avanti Mini-Extruder (Avanti Polar Lipids).

Concentration Assay:

Article Title: Mutual effects of MinD-membrane interaction: II. Domain structure of the membrane enhances MinD binding
Article Snippet: LUVs were prepared by extruding the suspension through a polycarbonate membrane filter (0.1 μm pore size, 19 mm diameter) 11 times using Avanti Mini-Extruder (Avanti Polar Lipids, Inc). .. Phospholipids concentration was determined by phosphomolybdate colorimetric assay [ ].

Article Title: First Experimental Evidence of Dopamine Interactions with Negatively Charged Model Biomembranes
Article Snippet: Subsequently, the thin film of lipid was suspended in an appropriate amount of warm 50 mM phosphate buffer (pH 7.2) to give a final lipid concentration 20 mM (by intensive vortexing at temperature above the lipid phase transition). .. In order to obtain LUVs from MLVs, suspensions of MLVs were extruded at least 21 times through a Nucleopore polycarbonate membrane (Whatman, pore diameter of 100 nm) in Avanti Mini-Extruder (Avanti Polar Lipids Inc.).

Article Title: Nuclear accumulation of plasmid DNA can be enhanced by non-selective gating of the nuclear pore
Article Snippet: The dried lipids were then hydrated by adding Hepes buffer till a final lipid concentration of 10.2 mM. .. Thereafter, the formed liposomes were extruded 11 times through two stacked 100 nm polycarbonate membrane filters (Whatman, Brentfort, UK) at room temperature using an Avanti Mini-Extruder (Avanti Polar Lipids).

Article Title: In Vitro Reconstitution of Functional Type III Protein Export and Insights into Flagellar Assembly
Article Snippet: Three hundred microliters of the frozen stock solution of IMVs was thawed at room temperature and homogenized 10 times through an 0.8-µm polycarbonate membrane with the Avanti Mini-Extruder (Avanti Polar Lipids). .. The ATP solution was prepared by dissolving ATP (dipotassium salt) in Tris-HCl solution (final concentration of 20 mM) followed by neutralization with KOH.

Article Title: Inhibition of NADPH oxidase by glucosylceramide confers chemoresistance
Article Snippet: Aliquots of lipids were made at appropriate ratios , and dried to a film under a stream of nitrogen, then hydrated by addition of 0.9% NaCl to a final lipid concentration of 25 mg/ml. .. The lipid vesicle-containing solution was quickly extruded at 60°C by passing the solution 10 times through 100 nm polycarbonate filters in an Avanti Mini-Extruder (Avanti Polar Lipids, Alabaster, AL).

Article Title: Dimyristoylphosphotidylcholine induces conformational changes in apoB that lowers lipoprotein(a)
Article Snippet: The DMPC vesicles were extruded through a polycarbonate membrane with a pore size of 30 nm using the Avanti Mini extruder (Avanti Polar Lipid). .. The concentration of phospholipid was determined using the Wako phospholipid assay kit.

Article Title: Plasma Exosomes Contribute to Microvascular Damage in Diabetic Retinopathy by Activating the Classical Complement Pathway
Article Snippet: .. Microvesicles of known size, composition, and concentration were extruded using an Avanti Mini-Extruder (Avanti Polar Lipids, Inc.) with 100-nm pore filters; these microvesicles were used to create calibration curves (particles per milliliter). .. DLS readings of extruded vesicles were compared with DLS readings of isolated mouse plasma exosomes ( ).

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    Avanti Polar mini extrudor
    Mini Extrudor, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mini extrudor/product/Avanti Polar
    Average 78 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mini extrudor - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

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