automatic dna sequencer model 373a  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 79

    Structured Review

    Thermo Fisher automatic dna sequencer model 373a
    Automatic Dna Sequencer Model 373a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/automatic dna sequencer model 373a/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    automatic dna sequencer model 373a - by Bioz Stars, 2020-02
    79/100 stars

    Related Products / Commonly Used Together

    abi prism bigdye primer cycle sequencing

    Images

    Related Articles

    Transfection:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Luciferase reporter plasmids were prepared for transfection using the EndoFree plasmid maxi kit (Qiagen). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Luciferase:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Paragraph title: Luciferase reporter constructs and expression plasmids ... The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Ligation:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Deletion constructs containing –577/+125 bp, –403/+125 bp, –131/+125 bp, –90/+125 bp were prepared by restriction enzyme digestion of pGL2-COL2 at PstI, NheI, NcoI, ApaI, and PvuII sites, respectively, followed by ligation. .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Mutagenesis:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Mutations in ETS sites in the pGL2-COL2 construct were generated using the PCR-based QuikChange™ Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Construct:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems). .. Transient transfection experiments were carried out in C-28/I2 cells using LipofectAMINE PLUS™ Reagent (Invitrogen).

    Sequencing:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems). .. Transient transfection experiments were carried out in C-28/I2 cells using LipofectAMINE PLUS™ Reagent (Invitrogen).

    Polymerase Chain Reaction:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Mutations in ETS sites in the pGL2-COL2 construct were generated using the PCR-based QuikChange™ Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Generated:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Mutations in ETS sites in the pGL2-COL2 construct were generated using the PCR-based QuikChange™ Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Activity Assay:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Mutation of the translation start site ATG to CTG in the pGL2-COL2 construct did not increase the activity indicating that splicing occurred correctly and did not interfere with translation of the luciferase reporter. .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    DNA Sequencing:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems). .. Transient transfection experiments were carried out in C-28/I2 cells using LipofectAMINE PLUS™ Reagent (Invitrogen).

    Expressing:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Paragraph title: Luciferase reporter constructs and expression plasmids ... The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    CTG Assay:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Mutation of the translation start site ATG to CTG in the pGL2-COL2 construct did not increase the activity indicating that splicing occurred correctly and did not interfere with translation of the luciferase reporter. .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Plasmid Preparation:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Luciferase reporter plasmids were prepared for transfection using the EndoFree plasmid maxi kit (Qiagen). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher dna sequencer
    Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 849 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna sequencer/product/Thermo Fisher
    Average 99 stars, based on 849 article reviews
    Price from $9.99 to $1999.99
    dna sequencer - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    Image Search Results