aur3 15075c18 ts  (IonOpticks)

Journal: STAR Protocols

Article Title: Protocol for high-throughput semi-automated label-free- or TMT-based phosphoproteome profiling

doi: 10.1016/j.xpro.2023.102536

Figure Lengend Snippet: Reference table for Evosep gradients and column set-up

Article Snippet: Figure 7 Expected performance of column and IMAC bead type DIA based phosphopeptide analysis using WHISPER 40SPD Evosep gradient (A) Comparative bar plot assessing the effect of the column type (Evosep (EV1112) vs. IonOpticks (AUR3-15075C18-TS)) on the number of phosphopeptides identified when using 2.5 μg of peptide input for phosphopeptide enrichment with MagReSyn Ti-IMAC-HP beads with the 40 SPD gradient (n = 3). (B) Comparative bar plot assessing the effect of the beads type (Ti-IMAC-HP vs. Zr-IMAC-HP) on the number of phosphopeptides (class I) identified when using 2.5 μg of peptide input for phosphopeptide enrichment using an IonOpticks column (n = 3) for phosphopeptide separation.

Techniques:

Journal: STAR Protocols

Article Title: Protocol for high-throughput semi-automated label-free- or TMT-based phosphoproteome profiling

doi: 10.1016/j.xpro.2023.102536

Figure Lengend Snippet: Expected performance of column and IMAC bead type DIA based phosphopeptide analysis using WHISPER 40SPD Evosep gradient (A) Comparative bar plot assessing the effect of the column type (Evosep (EV1112) vs. IonOpticks (AUR3-15075C18-TS)) on the number of phosphopeptides identified when using 2.5 μg of peptide input for phosphopeptide enrichment with MagReSyn Ti-IMAC-HP beads with the 40 SPD gradient (n = 3). (B) Comparative bar plot assessing the effect of the beads type (Ti-IMAC-HP vs. Zr-IMAC-HP) on the number of phosphopeptides (class I) identified when using 2.5 μg of peptide input for phosphopeptide enrichment using an IonOpticks column (n = 3) for phosphopeptide separation. Adapted from Martinez-Val et al .

Article Snippet: Figure 7 Expected performance of column and IMAC bead type DIA based phosphopeptide analysis using WHISPER 40SPD Evosep gradient (A) Comparative bar plot assessing the effect of the column type (Evosep (EV1112) vs. IonOpticks (AUR3-15075C18-TS)) on the number of phosphopeptides identified when using 2.5 μg of peptide input for phosphopeptide enrichment with MagReSyn Ti-IMAC-HP beads with the 40 SPD gradient (n = 3). (B) Comparative bar plot assessing the effect of the beads type (Ti-IMAC-HP vs. Zr-IMAC-HP) on the number of phosphopeptides (class I) identified when using 2.5 μg of peptide input for phosphopeptide enrichment using an IonOpticks column (n = 3) for phosphopeptide separation.

Techniques:

Journal: STAR Protocols

Article Title: Protocol for high-throughput semi-automated label-free- or TMT-based phosphoproteome profiling

doi: 10.1016/j.xpro.2023.102536

Figure Lengend Snippet:

Article Snippet: Figure 7 Expected performance of column and IMAC bead type DIA based phosphopeptide analysis using WHISPER 40SPD Evosep gradient (A) Comparative bar plot assessing the effect of the column type (Evosep (EV1112) vs. IonOpticks (AUR3-15075C18-TS)) on the number of phosphopeptides identified when using 2.5 μg of peptide input for phosphopeptide enrichment with MagReSyn Ti-IMAC-HP beads with the 40 SPD gradient (n = 3). (B) Comparative bar plot assessing the effect of the beads type (Ti-IMAC-HP vs. Zr-IMAC-HP) on the number of phosphopeptides (class I) identified when using 2.5 μg of peptide input for phosphopeptide enrichment using an IonOpticks column (n = 3) for phosphopeptide separation.

Techniques: Recombinant, Modification, Sequencing, Bicinchoninic Acid Protein Assay, Software, Cell Culture, Spectrophotometry, Extraction, Blocking Assay, Transferring, High Performance Liquid Chromatography, Mass Spectrometry