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Promega celltiter glo ctg luminescent assay kit
Effects of MMO on the cell viability of NAFLD model cells. The NAFLD model cells were treated with MMO (10 and 20 mg/mL) for 24 h, and cell viability was determined by <t>CellTiter-Glo</t> assay. Data are presented as mean ± S.D. for three independent experiments with triplicate determination. * p
Celltiter Glo Ctg Luminescent Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 1388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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celltiter glo ctg luminescent assay kit - by Bioz Stars, 2020-04
94/100 stars

Images

1) Product Images from "Protective Effects and Mechanism of Meretrix meretrix Oligopeptides against Nonalcoholic Fatty Liver Disease"

Article Title: Protective Effects and Mechanism of Meretrix meretrix Oligopeptides against Nonalcoholic Fatty Liver Disease

Journal: Marine Drugs

doi: 10.3390/md15020031

Effects of MMO on the cell viability of NAFLD model cells. The NAFLD model cells were treated with MMO (10 and 20 mg/mL) for 24 h, and cell viability was determined by CellTiter-Glo assay. Data are presented as mean ± S.D. for three independent experiments with triplicate determination. * p
Figure Legend Snippet: Effects of MMO on the cell viability of NAFLD model cells. The NAFLD model cells were treated with MMO (10 and 20 mg/mL) for 24 h, and cell viability was determined by CellTiter-Glo assay. Data are presented as mean ± S.D. for three independent experiments with triplicate determination. * p

Techniques Used: Glo Assay

2) Product Images from "Toxicity evaluation of manufactured CeO2 nanoparticles before and after alteration: combined physicochemical and whole-genome expression analysis in Caco-2 cells"

Article Title: Toxicity evaluation of manufactured CeO2 nanoparticles before and after alteration: combined physicochemical and whole-genome expression analysis in Caco-2 cells

Journal: BMC Genomics

doi: 10.1186/1471-2164-15-700

Caco-2 cell viability tests. Caco-2 cells were grown in 96-well plates and differentiated for 21 days. Cells were then exposed for 24 h or 72 h to concentrations of CeO 2 NPs ranging from 21.25 to 170 μg/mL. Left side) ATP tests: cell viability was determined by reading the level of bioluminescence (CellTiter-Glo luminescent cell viability assay, Promega). Right side) XTT tests: cell viability was determined by mitochondrial enzyme activity via XTT reagent (In Vitro toxicology assay kit XTT based, Sigma-Aldrich). An experimental positive control was obtained by exposing cells to H 2 O 2 in both tests. Cell viability was not altered for concentrations up to 170 mg/L.
Figure Legend Snippet: Caco-2 cell viability tests. Caco-2 cells were grown in 96-well plates and differentiated for 21 days. Cells were then exposed for 24 h or 72 h to concentrations of CeO 2 NPs ranging from 21.25 to 170 μg/mL. Left side) ATP tests: cell viability was determined by reading the level of bioluminescence (CellTiter-Glo luminescent cell viability assay, Promega). Right side) XTT tests: cell viability was determined by mitochondrial enzyme activity via XTT reagent (In Vitro toxicology assay kit XTT based, Sigma-Aldrich). An experimental positive control was obtained by exposing cells to H 2 O 2 in both tests. Cell viability was not altered for concentrations up to 170 mg/L.

Techniques Used: Cell Viability Assay, Activity Assay, In Vitro, Positive Control

3) Product Images from "The second extracellular loop dictates Occludin-mediated HCV entry"

Article Title: The second extracellular loop dictates Occludin-mediated HCV entry

Journal: Virology

doi: 10.1016/j.virol.2010.08.009

Dynasore has no adverse effect on cell viability. PHHs were treated with Dynasore (80μM) for 0, 1, 2, 4, 16, 24 hours. After removal of Dynasore, cells were incubated for additional 48 hours prior to the CellTiter- Glo cell viability assay (see
Figure Legend Snippet: Dynasore has no adverse effect on cell viability. PHHs were treated with Dynasore (80μM) for 0, 1, 2, 4, 16, 24 hours. After removal of Dynasore, cells were incubated for additional 48 hours prior to the CellTiter- Glo cell viability assay (see

Techniques Used: Incubation, Viability Assay

4) Product Images from "Intestinal toxicity evaluation of TiO2 degraded surface-treated nanoparticles: a combined physico-chemical and toxicogenomics approach in caco-2 cells"

Article Title: Intestinal toxicity evaluation of TiO2 degraded surface-treated nanoparticles: a combined physico-chemical and toxicogenomics approach in caco-2 cells

Journal: Particle and Fibre Toxicology

doi: 10.1186/1743-8977-9-18

Viability of Caco-2 cells exposed for 4 h, 24 h or 72 h toTiO 2 STNPs. Caco-2 cells were grown in a 96-well plate and differentiated for 21 days. The cells were then exposed for 4 h, 24 h or 72 h at TiO 2 STNPs concentrations ranging from 10 to 100 μl/mL. A) Cell viability was determined by intracellular ATP content ( CellTiter-Glo luminescent cell viability Assay, Promega). B) Cell viability was determined by mitochondrial enzyme activity via XTT reagent (In Vitro toxicology assay kit XTT based, Sigma-Aldrich).
Figure Legend Snippet: Viability of Caco-2 cells exposed for 4 h, 24 h or 72 h toTiO 2 STNPs. Caco-2 cells were grown in a 96-well plate and differentiated for 21 days. The cells were then exposed for 4 h, 24 h or 72 h at TiO 2 STNPs concentrations ranging from 10 to 100 μl/mL. A) Cell viability was determined by intracellular ATP content ( CellTiter-Glo luminescent cell viability Assay, Promega). B) Cell viability was determined by mitochondrial enzyme activity via XTT reagent (In Vitro toxicology assay kit XTT based, Sigma-Aldrich).

Techniques Used: Cell Viability Assay, Activity Assay, In Vitro

5) Product Images from "Inhibitors of deacetylases suppress oncogenic KIT signaling, acetylate HSP90, and induce apoptosis in gastrointestinal stromal tumors"

Article Title: Inhibitors of deacetylases suppress oncogenic KIT signaling, acetylate HSP90, and induce apoptosis in gastrointestinal stromal tumors

Journal: Cancer research

doi: 10.1158/0008-5472.CAN-08-4004

A: CellTiter-Glo ATP-based viability assays for SAHA in imatinib-sensitive (GIST882, GIST-T1) and imatinib-resistant (GIST48, GIST48B, GIST62, GIST522) cell lines. All cells were treated with the indicated concentrations and assessed after 3 days of treatment,
Figure Legend Snippet: A: CellTiter-Glo ATP-based viability assays for SAHA in imatinib-sensitive (GIST882, GIST-T1) and imatinib-resistant (GIST48, GIST48B, GIST62, GIST522) cell lines. All cells were treated with the indicated concentrations and assessed after 3 days of treatment,

Techniques Used:

Related Articles

Incubation:

Article Title: A Proposal for a Structural Model of the Feline Calicivirus Protease Bound to the Substrate Peptide under Physiological Conditions
Article Snippet: Assessment of Antiviral and Cytotoxic Effects of Compound No. 108 Confluent monolayers of CRFK cells in 6-well plates (approximately 106 cells/well) were inoculated at a MOI of 0.0001 with the FCV F4 strain or FCV 2280 strain isolated in the United States ( ) (Genbank accession no. KC835209) and then incubated for 2 h. The cells were washed once with PBS without Ca2+ and Mg2+ [PBS (-)] and incubated in 2 mL of serum free medium including compound No. 108 (25 or 12.5 μM) or DMSO at 37°C up to 40 h. The virus titers in the supernatant were determined by the 50% tissue culture infective dose (TCID50 ) method as described previously ( ). .. For evaluation of the cytotoxic effect, the CRFK cells were cultured in the presence of increasing concentrations of a given chemical compound for 48 h. The cell variability was quantified by a CellTiter Glo Luminescent Cell Viability Assay Kit (Promega) according to the manufacturer’s instructions.

Microarray:

Article Title: Glutamate Receptor GRIA3--Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer 1Glutamate Receptor GRIA3--Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer 1 2
Article Snippet: A total of 41 genes were selected as putative CUX1 targets, which were differentially expressed in a genome-wide microarray profile for CUX1 target genes published previously [ ]. .. Forty-eight hours later, cell viability was measured using CellTiter-Glo luminescent assay (Promega, Madison, WI) according to the manufacturer's instructions.

Quantitation Assay:

Article Title: Short-chain fatty acids induced autophagy serves as an adaptive strategy for retarding mitochondria-mediated apoptotic cell death
Article Snippet: Paragraph title: ATP quantitation ... ATP was quantitated with a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega Corporation).

Proliferation Assay:

Article Title: Combination treatment of RAD001 and BEZ235 exhibits synergistic antitumor activity via down-regulation of p-4E-BP1/Mcl-1 in small cell lung cancer
Article Snippet: Paragraph title: Cell proliferation assay ... Cell viability was determined by CellTiter-Glo Luminescent assay (Promega, Madison, WI, USA).

Article Title: Chromogranin A regulates neuroblastoma proliferation and phenotype
Article Snippet: Paragraph title: CellTiter-Glo® proliferation assay ... Cell viability was determined using CellTiter-Glo® Luminescent Cell Viability Assay kit (Promega) with a luminometer (Wallac 1420 Victor 2 multipliable counter system).

Genome Wide:

Article Title: Glutamate Receptor GRIA3--Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer 1Glutamate Receptor GRIA3--Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer 1 2
Article Snippet: A total of 41 genes were selected as putative CUX1 targets, which were differentially expressed in a genome-wide microarray profile for CUX1 target genes published previously [ ]. .. Forty-eight hours later, cell viability was measured using CellTiter-Glo luminescent assay (Promega, Madison, WI) according to the manufacturer's instructions.

Transfection:

Article Title: Predominant requirement of Bax for apoptosis in HCT116 cells is determined by Mcl-1's inhibitory effect on Bak
Article Snippet: For transfection, 2.5×104 cells were seeded and transfected with Lipofectamine LTX (Invitrogen). .. Cell viability and caspse-3/7 activity were measured with CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, Madison, WI) and Caspase-Glo 3/7 Assay kit (Promega), respectively.

Article Title: MicroRNA-192 targeting retinoblastoma 1 inhibits cell proliferation and induces cell apoptosis in lung cancer cells
Article Snippet: Forty-eight hours after transfection, 20 µl of MTT (5 mg/ml) was added to each well of a 96-well plate and the cells were cultured for 4 h. The MTT medium mixture was then discarded and 150 µl of dimethyl sulfoxide (DMSO) was added to each well. .. Cell viability was measured using the Celltiter-Glo luminescent cell viability assay kit (Promega, USA) according to the manufacturer's protocol.

Article Title: Discovering cancer vulnerabilities using high-throughput micro-RNA screening
Article Snippet: High-throughput micro-RNA screening All cell lines were reverse transfected in 384-well plate format with 25 nM of Dharmacon miRNA mimic or inhibitor libraries (version 16.0) using a Calliper Sciclone ALH3000 liquid-handling robot according to the conditions listed in . .. After 24 h, media was changed using a BioTek 406 automated plate washer/dispenser, and 6 days posttransfection, cell viability was determined using the CellTiter-Glo (CTG) luminescent assay (Promega) at 1:2 dilution; luminescence measurements were taken on the Synergy H4 (BioTek) high-throughput multimode micro-plate reader.

Article Title: Glutamate Receptor GRIA3--Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer 1Glutamate Receptor GRIA3--Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer 1 2
Article Snippet: After 24 hours, cells were transfected with siRNA using siLentFect Lipid Reagent (BioRad, Munich, Germany) following the manufacturer's instructions. .. Forty-eight hours later, cell viability was measured using CellTiter-Glo luminescent assay (Promega, Madison, WI) according to the manufacturer's instructions.

Cell Culture:

Article Title: MicroRNA-192 targeting retinoblastoma 1 inhibits cell proliferation and induces cell apoptosis in lung cancer cells
Article Snippet: Forty-eight hours after transfection, 20 µl of MTT (5 mg/ml) was added to each well of a 96-well plate and the cells were cultured for 4 h. The MTT medium mixture was then discarded and 150 µl of dimethyl sulfoxide (DMSO) was added to each well. .. Cell viability was measured using the Celltiter-Glo luminescent cell viability assay kit (Promega, USA) according to the manufacturer's protocol.

Article Title: Contemporary Circulating Enterovirus D68 Strains Have Acquired the Capacity for Viral Entry and Replication in Human Neuronal Cells
Article Snippet: Cells were cultured and evaluated in the same manner as in the viral replication kinetics assays. .. ATP levels were measured using the CellTiter-Glo luminescent cell viability assay kit (catalog no. G7570; Promega), and cell viability was calculated relative to that of the mock control.

Article Title: Chromogranin A regulates neuroblastoma proliferation and phenotype
Article Snippet: CellTiter-Glo® proliferation assay Cells were suspended in 100 μl DMEM supplemented with 10% FBS, and plated in 96-well plates (2×104 viable cells/well) and cultured overnight. .. Cell viability was determined using CellTiter-Glo® Luminescent Cell Viability Assay kit (Promega) with a luminometer (Wallac 1420 Victor 2 multipliable counter system).

Article Title: A Proposal for a Structural Model of the Feline Calicivirus Protease Bound to the Substrate Peptide under Physiological Conditions
Article Snippet: .. For evaluation of the cytotoxic effect, the CRFK cells were cultured in the presence of increasing concentrations of a given chemical compound for 48 h. The cell variability was quantified by a CellTiter Glo Luminescent Cell Viability Assay Kit (Promega) according to the manufacturer’s instructions. ..

Luminescence Assay:

Article Title: Discovering cancer vulnerabilities using high-throughput micro-RNA screening
Article Snippet: .. After 24 h, media was changed using a BioTek 406 automated plate washer/dispenser, and 6 days posttransfection, cell viability was determined using the CellTiter-Glo (CTG) luminescent assay (Promega) at 1:2 dilution; luminescence measurements were taken on the Synergy H4 (BioTek) high-throughput multimode micro-plate reader. .. On each library plate we included a large number of positive and negative technical controls, including non-targeting ON-TARGETplus siRNA SMARTPool (NT), lipid-only mock transfection, and mimic and inhibitor non-targeting controls.

Article Title: Glutamate Receptor GRIA3--Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer 1Glutamate Receptor GRIA3--Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer 1 2
Article Snippet: .. Forty-eight hours later, cell viability was measured using CellTiter-Glo luminescent assay (Promega, Madison, WI) according to the manufacturer's instructions. ..

Article Title: Polo-like kinase 1 inhibition diminishes acquired resistance to epidermal growth factor receptor inhibition in non-small cell lung cancer with T790M mutations
Article Snippet: .. After 72 h of treatment, cell viability was estimated using the CellTiter-Glo luminescent assay (Promega, Madison, WI) as previously described [ ]. ..

Article Title: Combination treatment of RAD001 and BEZ235 exhibits synergistic antitumor activity via down-regulation of p-4E-BP1/Mcl-1 in small cell lung cancer
Article Snippet: .. Cell viability was determined by CellTiter-Glo Luminescent assay (Promega, Madison, WI, USA). ..

Article Title: Establishment and characterization of a novel MYC/BCL2 “double-hit” diffuse large B cell lymphoma cell line, RC
Article Snippet: .. Cell growth and viability assay Cell viability was assessed using the CellTiter-Glo Luminescent Assay (Promega). .. Cells were plated in triplicates at 1–2 × 104 cells/well in 96-well plate with increase concentrations of AZD8055 (Selleckchem) in 100 μl total volume.

MTT Assay:

Article Title: MicroRNA-192 targeting retinoblastoma 1 inhibits cell proliferation and induces cell apoptosis in lung cancer cells
Article Snippet: Forty-eight hours after transfection, 20 µl of MTT (5 mg/ml) was added to each well of a 96-well plate and the cells were cultured for 4 h. The MTT medium mixture was then discarded and 150 µl of dimethyl sulfoxide (DMSO) was added to each well. .. Cell viability was measured using the Celltiter-Glo luminescent cell viability assay kit (Promega, USA) according to the manufacturer's protocol.

Article Title: Antiviral activity of micafungin against enterovirus 71
Article Snippet: .. Cell toxicity assay MTT assay or CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega) was used for measuring cell toxicity as described previously [ ]. ..

Article Title: Kaempferol mitigates Endoplasmic Reticulum Stress Induced Cell Death by targeting caspase 3/7
Article Snippet: All the test compounds were screened at a single concentration of 50 µM and MTT assay was performed to assess the percentage of cell survival. .. The cytoprotective activity of kaempferol was confirmed by an orthogonal assay that measures the ATP level (CellTiter-Glo luminescent cell viability assay, Promega, India) and trypan blue dye exclusion assay as a surrogate for the cell viability (Fig. and Supplementary Figure 4a).

Isolation:

Article Title: A Proposal for a Structural Model of the Feline Calicivirus Protease Bound to the Substrate Peptide under Physiological Conditions
Article Snippet: Assessment of Antiviral and Cytotoxic Effects of Compound No. 108 Confluent monolayers of CRFK cells in 6-well plates (approximately 106 cells/well) were inoculated at a MOI of 0.0001 with the FCV F4 strain or FCV 2280 strain isolated in the United States ( ) (Genbank accession no. KC835209) and then incubated for 2 h. The cells were washed once with PBS without Ca2+ and Mg2+ [PBS (-)] and incubated in 2 mL of serum free medium including compound No. 108 (25 or 12.5 μM) or DMSO at 37°C up to 40 h. The virus titers in the supernatant were determined by the 50% tissue culture infective dose (TCID50 ) method as described previously ( ). .. For evaluation of the cytotoxic effect, the CRFK cells were cultured in the presence of increasing concentrations of a given chemical compound for 48 h. The cell variability was quantified by a CellTiter Glo Luminescent Cell Viability Assay Kit (Promega) according to the manufacturer’s instructions.

Mouse Assay:

Article Title: Increased Hepatic Fatty Acids Uptake and Oxidation by LRPPRC-Driven Oxidative Phosphorylation Reduces Blood Lipid Levels
Article Snippet: All lysates were adjusted to the same μg liver / μl before 50 μl lysate being used for measurement using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, Madison, WI) according to manufacturer's protocol. .. The average liver ATP levels of Lrpprc Tg or Lrpprc knockdown mice were normalized against their respective control mice.

CTG Assay:

Article Title: Discovering cancer vulnerabilities using high-throughput micro-RNA screening
Article Snippet: .. After 24 h, media was changed using a BioTek 406 automated plate washer/dispenser, and 6 days posttransfection, cell viability was determined using the CellTiter-Glo (CTG) luminescent assay (Promega) at 1:2 dilution; luminescence measurements were taken on the Synergy H4 (BioTek) high-throughput multimode micro-plate reader. .. On each library plate we included a large number of positive and negative technical controls, including non-targeting ON-TARGETplus siRNA SMARTPool (NT), lipid-only mock transfection, and mimic and inhibitor non-targeting controls.

Small Interfering RNA:

Article Title: Glutamate Receptor GRIA3--Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer 1Glutamate Receptor GRIA3--Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer 1 2
Article Snippet: Three small interfering RNA (siRNA) duplexes from Ambion (Austin, TX) with independent silencing sequences were used for each gene. .. Forty-eight hours later, cell viability was measured using CellTiter-Glo luminescent assay (Promega, Madison, WI) according to the manufacturer's instructions.

Caspase-Glo Assay:

Article Title: Predominant requirement of Bax for apoptosis in HCT116 cells is determined by Mcl-1's inhibitory effect on Bak
Article Snippet: .. Cell viability and caspse-3/7 activity were measured with CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, Madison, WI) and Caspase-Glo 3/7 Assay kit (Promega), respectively. .. Supplementary Material Figure S1: Clustering of GFP-Bak on mitochondria during apoptosis induced by TRAIL plus 5-FU in Bak−/− and Bax−/− Bak−/− HCT116 cells stablying expressing GFP-Bak.

Viability Assay:

Article Title: Antitumor Efficacy of the Dual PI3K/mTOR Inhibitor PF-04691502 in a Human Xenograft Tumor Model Derived from Colorectal Cancer Stem Cells Harboring a PIK3CA Mutation
Article Snippet: Paragraph title: Cell Viability Assay after Treatment with Compound ... Cell viability was determined by using a CellTiter Glo® luminescent cell viability assay kit (Promega).

Article Title: Establishment and characterization of a novel MYC/BCL2 “double-hit” diffuse large B cell lymphoma cell line, RC
Article Snippet: .. Cell growth and viability assay Cell viability was assessed using the CellTiter-Glo Luminescent Assay (Promega). .. Cells were plated in triplicates at 1–2 × 104 cells/well in 96-well plate with increase concentrations of AZD8055 (Selleckchem) in 100 μl total volume.

Software:

Article Title: Combination treatment of RAD001 and BEZ235 exhibits synergistic antitumor activity via down-regulation of p-4E-BP1/Mcl-1 in small cell lung cancer
Article Snippet: Cell viability was determined by CellTiter-Glo Luminescent assay (Promega, Madison, WI, USA). .. The IC50 values were determined from the sigmoidal dose–response curves using PRISM4 software (GraphPad Software, Inc., La Jolla, CA, USA).

Exclusion Assay:

Article Title: Kaempferol mitigates Endoplasmic Reticulum Stress Induced Cell Death by targeting caspase 3/7
Article Snippet: .. The cytoprotective activity of kaempferol was confirmed by an orthogonal assay that measures the ATP level (CellTiter-Glo luminescent cell viability assay, Promega, India) and trypan blue dye exclusion assay as a surrogate for the cell viability (Fig. and Supplementary Figure 4a). ..

Apoptosis Assay:

Article Title: MicroRNA-192 targeting retinoblastoma 1 inhibits cell proliferation and induces cell apoptosis in lung cancer cells
Article Snippet: Paragraph title: Apoptosis assay ... Cell viability was measured using the Celltiter-Glo luminescent cell viability assay kit (Promega, USA) according to the manufacturer's protocol.

Concentration Assay:

Article Title: Kaempferol mitigates Endoplasmic Reticulum Stress Induced Cell Death by targeting caspase 3/7
Article Snippet: All the test compounds were screened at a single concentration of 50 µM and MTT assay was performed to assess the percentage of cell survival. .. The cytoprotective activity of kaempferol was confirmed by an orthogonal assay that measures the ATP level (CellTiter-Glo luminescent cell viability assay, Promega, India) and trypan blue dye exclusion assay as a surrogate for the cell viability (Fig. and Supplementary Figure 4a).

High Throughput Screening Assay:

Article Title: Discovering cancer vulnerabilities using high-throughput micro-RNA screening
Article Snippet: .. After 24 h, media was changed using a BioTek 406 automated plate washer/dispenser, and 6 days posttransfection, cell viability was determined using the CellTiter-Glo (CTG) luminescent assay (Promega) at 1:2 dilution; luminescence measurements were taken on the Synergy H4 (BioTek) high-throughput multimode micro-plate reader. .. On each library plate we included a large number of positive and negative technical controls, including non-targeting ON-TARGETplus siRNA SMARTPool (NT), lipid-only mock transfection, and mimic and inhibitor non-targeting controls.

FACS:

Article Title: MicroRNA-192 targeting retinoblastoma 1 inhibits cell proliferation and induces cell apoptosis in lung cancer cells
Article Snippet: Cell viability was measured using the Celltiter-Glo luminescent cell viability assay kit (Promega, USA) according to the manufacturer's protocol. .. Cell apoptosis was also detected using an Annexin V-PE apoptosis detection kit (BD, USA) according to the manufacturer's protocol and FACS analysis (BD, USA).

Cell Viability Assay:

Article Title: Predominant requirement of Bax for apoptosis in HCT116 cells is determined by Mcl-1's inhibitory effect on Bak
Article Snippet: .. Cell viability and caspse-3/7 activity were measured with CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, Madison, WI) and Caspase-Glo 3/7 Assay kit (Promega), respectively. .. Supplementary Material Figure S1: Clustering of GFP-Bak on mitochondria during apoptosis induced by TRAIL plus 5-FU in Bak−/− and Bax−/− Bak−/− HCT116 cells stablying expressing GFP-Bak.

Article Title: MicroRNA-192 targeting retinoblastoma 1 inhibits cell proliferation and induces cell apoptosis in lung cancer cells
Article Snippet: .. Cell viability was measured using the Celltiter-Glo luminescent cell viability assay kit (Promega, USA) according to the manufacturer's protocol. ..

Article Title: Antitumor Efficacy of the Dual PI3K/mTOR Inhibitor PF-04691502 in a Human Xenograft Tumor Model Derived from Colorectal Cancer Stem Cells Harboring a PIK3CA Mutation
Article Snippet: .. Cell viability was determined by using a CellTiter Glo® luminescent cell viability assay kit (Promega). .. Cell Proliferation and Differentiation Induction Cell proliferation and cytokeratin expression were measured simultaneously using a fluorescein isothiocyanate 5-bromodeoxyuridine (BrdU) flow kit (BD Biosciences) and anti-cytokeratin antibodies (phycoerythrin-conjugated cytokeratin 7/8 CAM 5.2, BD Biosciences).

Article Title: Antiviral activity of micafungin against enterovirus 71
Article Snippet: .. Cell toxicity assay MTT assay or CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega) was used for measuring cell toxicity as described previously [ ]. ..

Article Title: Increased Hepatic Fatty Acids Uptake and Oxidation by LRPPRC-Driven Oxidative Phosphorylation Reduces Blood Lipid Levels
Article Snippet: .. All lysates were adjusted to the same μg liver / μl before 50 μl lysate being used for measurement using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, Madison, WI) according to manufacturer's protocol. .. The luminescent signal was detected using a POLARstar Omega microplate reader (BMG Labtech, Offenburg, Germany).

Article Title: Kaempferol mitigates Endoplasmic Reticulum Stress Induced Cell Death by targeting caspase 3/7
Article Snippet: .. The cytoprotective activity of kaempferol was confirmed by an orthogonal assay that measures the ATP level (CellTiter-Glo luminescent cell viability assay, Promega, India) and trypan blue dye exclusion assay as a surrogate for the cell viability (Fig. and Supplementary Figure 4a). ..

Article Title: Short-chain fatty acids induced autophagy serves as an adaptive strategy for retarding mitochondria-mediated apoptotic cell death
Article Snippet: .. ATP was quantitated with a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega Corporation). .. Luminescence was measured by Luminoskan Ascent from Thermo Scientific.

Article Title: Contemporary Circulating Enterovirus D68 Strains Have Acquired the Capacity for Viral Entry and Replication in Human Neuronal Cells
Article Snippet: .. ATP levels were measured using the CellTiter-Glo luminescent cell viability assay kit (catalog no. G7570; Promega), and cell viability was calculated relative to that of the mock control. ..

Article Title: Chromogranin A regulates neuroblastoma proliferation and phenotype
Article Snippet: .. Cell viability was determined using CellTiter-Glo® Luminescent Cell Viability Assay kit (Promega) with a luminometer (Wallac 1420 Victor 2 multipliable counter system). ..

Article Title: A Proposal for a Structural Model of the Feline Calicivirus Protease Bound to the Substrate Peptide under Physiological Conditions
Article Snippet: .. For evaluation of the cytotoxic effect, the CRFK cells were cultured in the presence of increasing concentrations of a given chemical compound for 48 h. The cell variability was quantified by a CellTiter Glo Luminescent Cell Viability Assay Kit (Promega) according to the manufacturer’s instructions. ..

Activity Assay:

Article Title: Predominant requirement of Bax for apoptosis in HCT116 cells is determined by Mcl-1's inhibitory effect on Bak
Article Snippet: .. Cell viability and caspse-3/7 activity were measured with CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, Madison, WI) and Caspase-Glo 3/7 Assay kit (Promega), respectively. .. Supplementary Material Figure S1: Clustering of GFP-Bak on mitochondria during apoptosis induced by TRAIL plus 5-FU in Bak−/− and Bax−/− Bak−/− HCT116 cells stablying expressing GFP-Bak.

Article Title: Kaempferol mitigates Endoplasmic Reticulum Stress Induced Cell Death by targeting caspase 3/7
Article Snippet: .. The cytoprotective activity of kaempferol was confirmed by an orthogonal assay that measures the ATP level (CellTiter-Glo luminescent cell viability assay, Promega, India) and trypan blue dye exclusion assay as a surrogate for the cell viability (Fig. and Supplementary Figure 4a). ..

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    Promega atp bioluminescence assay kit celltiter glo luminescent cell viability assay
    Atp Bioluminescence Assay Kit Celltiter Glo Luminescent Cell Viability Assay, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega atp bioluminescence assay kit
    Atp Bioluminescence Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp bioluminescence assay kit/product/Promega
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
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