atp α 32p easytide lead  (PerkinElmer)

 
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    Name:
    ATP α 32P EasyTide Lead
    Description:
    Adenosine triphosphate labeled on the alpha phosphate group with 32P Common applications include RNA labeling Labeling with SP6 RNA polymerase T3 RNA polymerase T7 RNA polymerase BLU products are packaged in a lead free container “pig NEG products are packaged in a lead lined container plastic pig containing a layer of lead
    Catalog Number:
    neg503h250uc
    Price:
    None
    Size:
    250 µCi
    Category:
    Reagents
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    Structured Review

    PerkinElmer atp α 32p easytide lead
    ATP α 32P EasyTide Lead
    Adenosine triphosphate labeled on the alpha phosphate group with 32P Common applications include RNA labeling Labeling with SP6 RNA polymerase T3 RNA polymerase T7 RNA polymerase BLU products are packaged in a lead free container “pig NEG products are packaged in a lead lined container plastic pig containing a layer of lead
    https://www.bioz.com/result/atp α 32p easytide lead/product/PerkinElmer
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atp α 32p easytide lead - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    ATPase Assay:

    Article Title: A Functional DnaK Dimer Is Essential for the Efficient Interaction with Hsp40 Heat Shock Protein *
    Article Snippet: Paragraph title: Single-turnover ATPase Assay ... Briefly, 20 μg of DnaK protein was incubated with 25 μCi of [α-32 P]ATP (NEG503H250UC, 3000 Ci/mmol; PerkinElmer Life Sciences) in buffer C in the presence of 20 μ m unlabeled ATP for 5 min on ice.

    Article Title: Conformation transitions of the polypeptide-binding pocket support an active substrate release from Hsp70s
    Article Snippet: Paragraph title: Single-turnover ATPase assay ... Briefly, 2 μg of purified BiP and DnaK proteins was diluted with buffers B and C, respectively, and then incubated with 2 μl of [α-32P] ATP (NEG503H250UC, 3000 Ci/mmol; Perkin Elmer) in a final volume of 20 μl on ice for 2 min to allow ATP binding to these Hsp70 proteins.

    Article Title: A disulfide-bonded DnaK dimer is maintained in an ATP-bound state
    Article Snippet: Paragraph title: Single-turnover ATPase assay for rate of ATP hydrolysis ( k cat ) ... Briefly, DnaK proteins were incubated with of [α-32 P] ATP (NEG503H250UC, 3000 Ci/mmol; Perkin Elmer) in buffer A to form DnaK-ATP complex.

    Article Title: The Four Hydrophobic Residues on the Hsp70 Inter-Domain Linker Have Two Distinct Roles
    Article Snippet: Paragraph title: Single-turnover ATPase assay ... Single-turnover ATPase assays with DnaK proteins were performed as previously described, with modifications., To form a DnaK–ATP complex, we incubated the DnaK protein (20 μg) with 25 μCi of [α-32 P] ATP (NEG503H250UC, 3000 Ci/mmol; Perkin Elmer) in buffer A containing 20 μM unlabeled ATP for 2 min at 4 °C.

    Isolation:

    Article Title: A Functional DnaK Dimer Is Essential for the Efficient Interaction with Hsp40 Heat Shock Protein *
    Article Snippet: Briefly, 20 μg of DnaK protein was incubated with 25 μCi of [α-32 P]ATP (NEG503H250UC, 3000 Ci/mmol; PerkinElmer Life Sciences) in buffer C in the presence of 20 μ m unlabeled ATP for 5 min on ice. .. The DnaK-ATP complex was quickly isolated from free ATP on a spin column pre-equilibrated with buffer C. We started each reaction by mixing equal volumes of the DnaK-ATP complex with NR peptide, GrpE, or DnaJ in the indicated concentrations.

    Article Title: Conformation transitions of the polypeptide-binding pocket support an active substrate release from Hsp70s
    Article Snippet: Briefly, 2 μg of purified BiP and DnaK proteins was diluted with buffers B and C, respectively, and then incubated with 2 μl of [α-32P] ATP (NEG503H250UC, 3000 Ci/mmol; Perkin Elmer) in a final volume of 20 μl on ice for 2 min to allow ATP binding to these Hsp70 proteins. .. The Hsp70–ATP complex was quickly isolated from free ATP on a spin column pre-equilibrated with buffer B for BiP and Buffer C for DnaK, aliquoted and frozen in liquid nitrogen.

    Purification:

    Article Title: Conformation transitions of the polypeptide-binding pocket support an active substrate release from Hsp70s
    Article Snippet: .. Briefly, 2 μg of purified BiP and DnaK proteins was diluted with buffers B and C, respectively, and then incubated with 2 μl of [α-32P] ATP (NEG503H250UC, 3000 Ci/mmol; Perkin Elmer) in a final volume of 20 μl on ice for 2 min to allow ATP binding to these Hsp70 proteins. .. The Hsp70–ATP complex was quickly isolated from free ATP on a spin column pre-equilibrated with buffer B for BiP and Buffer C for DnaK, aliquoted and frozen in liquid nitrogen.

    Incubation:

    Article Title: A Functional DnaK Dimer Is Essential for the Efficient Interaction with Hsp40 Heat Shock Protein *
    Article Snippet: .. Briefly, 20 μg of DnaK protein was incubated with 25 μCi of [α-32 P]ATP (NEG503H250UC, 3000 Ci/mmol; PerkinElmer Life Sciences) in buffer C in the presence of 20 μ m unlabeled ATP for 5 min on ice. .. The DnaK-ATP complex was quickly isolated from free ATP on a spin column pre-equilibrated with buffer C. We started each reaction by mixing equal volumes of the DnaK-ATP complex with NR peptide, GrpE, or DnaJ in the indicated concentrations.

    Article Title: Conformation transitions of the polypeptide-binding pocket support an active substrate release from Hsp70s
    Article Snippet: .. Briefly, 2 μg of purified BiP and DnaK proteins was diluted with buffers B and C, respectively, and then incubated with 2 μl of [α-32P] ATP (NEG503H250UC, 3000 Ci/mmol; Perkin Elmer) in a final volume of 20 μl on ice for 2 min to allow ATP binding to these Hsp70 proteins. .. The Hsp70–ATP complex was quickly isolated from free ATP on a spin column pre-equilibrated with buffer B for BiP and Buffer C for DnaK, aliquoted and frozen in liquid nitrogen.

    Article Title: A disulfide-bonded DnaK dimer is maintained in an ATP-bound state
    Article Snippet: .. Briefly, DnaK proteins were incubated with of [α-32 P] ATP (NEG503H250UC, 3000 Ci/mmol; Perkin Elmer) in buffer A to form DnaK-ATP complex. ..

    Article Title: The Four Hydrophobic Residues on the Hsp70 Inter-Domain Linker Have Two Distinct Roles
    Article Snippet: .. Single-turnover ATPase assays with DnaK proteins were performed as previously described, with modifications., To form a DnaK–ATP complex, we incubated the DnaK protein (20 μg) with 25 μCi of [α-32 P] ATP (NEG503H250UC, 3000 Ci/mmol; Perkin Elmer) in buffer A containing 20 μM unlabeled ATP for 2 min at 4 °C. ..

    Thin Layer Chromatography:

    Article Title: A Functional DnaK Dimer Is Essential for the Efficient Interaction with Hsp40 Heat Shock Protein *
    Article Snippet: Briefly, 20 μg of DnaK protein was incubated with 25 μCi of [α-32 P]ATP (NEG503H250UC, 3000 Ci/mmol; PerkinElmer Life Sciences) in buffer C in the presence of 20 μ m unlabeled ATP for 5 min on ice. .. After stopping the reactions at the indicated time points, ATP was separated from ADP using PEI-cellulose thin layer chromatography plates (Sigma).

    Article Title: A disulfide-bonded DnaK dimer is maintained in an ATP-bound state
    Article Snippet: Briefly, DnaK proteins were incubated with of [α-32 P] ATP (NEG503H250UC, 3000 Ci/mmol; Perkin Elmer) in buffer A to form DnaK-ATP complex. .. After incubating at 20° for indicated time periods, samples were taken and PEI-cellulose thin-layer chromatography plates (Sigma-Aldrich) was used to separate ATP from ADP.

    Article Title: The Four Hydrophobic Residues on the Hsp70 Inter-Domain Linker Have Two Distinct Roles
    Article Snippet: Single-turnover ATPase assays with DnaK proteins were performed as previously described, with modifications., To form a DnaK–ATP complex, we incubated the DnaK protein (20 μg) with 25 μCi of [α-32 P] ATP (NEG503H250UC, 3000 Ci/mmol; Perkin Elmer) in buffer A containing 20 μM unlabeled ATP for 2 min at 4 °C. .. At specific time points, reactions were stopped, and the ATP was separated from ADP using thin-layer chromatography.

    Modification:

    Article Title: A disulfide-bonded DnaK dimer is maintained in an ATP-bound state
    Article Snippet: Single-turnover ATPase assay on all the DnaK proteins was performed practically as described previously with modification (Liu et al. ; Kumar et al. ). .. Briefly, DnaK proteins were incubated with of [α-32 P] ATP (NEG503H250UC, 3000 Ci/mmol; Perkin Elmer) in buffer A to form DnaK-ATP complex.

    Binding Assay:

    Article Title: Conformation transitions of the polypeptide-binding pocket support an active substrate release from Hsp70s
    Article Snippet: .. Briefly, 2 μg of purified BiP and DnaK proteins was diluted with buffers B and C, respectively, and then incubated with 2 μl of [α-32P] ATP (NEG503H250UC, 3000 Ci/mmol; Perkin Elmer) in a final volume of 20 μl on ice for 2 min to allow ATP binding to these Hsp70 proteins. .. The Hsp70–ATP complex was quickly isolated from free ATP on a spin column pre-equilibrated with buffer B for BiP and Buffer C for DnaK, aliquoted and frozen in liquid nitrogen.

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  • 99
    PerkinElmer atp α 32p easytide lead
    Atp α 32p Easytide Lead, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp α 32p easytide lead/product/PerkinElmer
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atp α 32p easytide lead - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    PerkinElmer datp α 32p easytide lead
    Datp α 32p Easytide Lead, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/datp α 32p easytide lead/product/PerkinElmer
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    datp α 32p easytide lead - by Bioz Stars, 2020-04
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