atg deficient frt hygro fusion gene (Thermo Fisher)
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Name:
pSecTag2 Hygro A B C Mammalian Expression Vectors
Description:
pSecTag2 and pSecTag2 Hygro are mammalian expression vectors designed for the secretion purification and detection of fusion proteins Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N terminal secretion signal The vectors Figure 1 offer the following features • Secretion signal from the V J2 C region of the mouse Ig kappa chain for efficient secretion of recombinant proteins Figure 2 • Cytomegalovirus CMV promoter for high level constitutive expression• C terminal polyhistidine 6xHis tag for rapid purification with nickel chelating resin and detection with an Anti His C term Antibody• C terminal c myc epitope for detection with an Anti myc Antibody• Bovine Growth Hormone BGH polyadenylation signal and transcription termination sequence to enhance mRNA stability• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen e g COS 1 COS 7 The pSecTag2 vectors carry the Zeocin resistance gene for cost effective selection in mammalian cells Zeocin selection can also be used in E coli The pSecTag2 Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines
Catalog Number:
v91020
Price:
None
Category:
DNA Vectors Clones Purified Nucleic Acids Libraries
Applications:
Constitutive Expression|Mammalian Expression|Protein Biology|Protein Expression|Secreted Expression
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Structured Review
Thermo Fisher
atg deficient frt hygro fusion gene
pSecTag2 and pSecTag2 Hygro are mammalian expression vectors designed for the secretion purification and detection of fusion proteins Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N terminal secretion signal The vectors Figure 1 offer the following features • Secretion signal from the V J2 C region of the mouse Ig kappa chain for efficient secretion of recombinant proteins Figure 2 • Cytomegalovirus CMV promoter for high level constitutive expression• C terminal polyhistidine 6xHis tag for rapid purification with nickel chelating resin and detection with an Anti His C term Antibody• C terminal c myc epitope for detection with an Anti myc Antibody• Bovine Growth Hormone BGH polyadenylation signal and transcription termination sequence to enhance mRNA stability• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen e g COS 1 COS 7 The pSecTag2 vectors carry the Zeocin resistance gene for cost effective selection in mammalian cells Zeocin selection can also be used in E coli The pSecTag2 Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines
https://www.bioz.com/result/atg deficient frt hygro fusion gene/product/Thermo Fisher
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pSecTag2 and pSecTag2 Hygro are mammalian expression vectors designed for the secretion purification and detection of fusion proteins Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N terminal secretion signal The vectors Figure 1 offer the following features • Secretion signal from the V J2 C region of the mouse Ig kappa chain for efficient secretion of recombinant proteins Figure 2 • Cytomegalovirus CMV promoter for high level constitutive expression• C terminal polyhistidine 6xHis tag for rapid purification with nickel chelating resin and detection with an Anti His C term Antibody• C terminal c myc epitope for detection with an Anti myc Antibody• Bovine Growth Hormone BGH polyadenylation signal and transcription termination sequence to enhance mRNA stability• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen e g COS 1 COS 7 The pSecTag2 vectors carry the Zeocin resistance gene for cost effective selection in mammalian cells Zeocin selection can also be used in E coli The pSecTag2 Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines
https://www.bioz.com/result/atg deficient frt hygro fusion gene/product/Thermo Fisher
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
atg deficient frt hygro fusion gene - by Bioz Stars,
2021-03
97/100 stars
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Construct:Article Title: A peptide derived from an extracellular domain selectively inhibits receptor internalization: Target sequences on insulin and insulin-like growth factor 1 receptors Article Snippet: IRp binding to IGF-1R was carried out in the presence or absence of 5 nM insulin or IGF-1. .. Deletion mutants were constructed using the PCR and standard cloning techniques , and subcloned into a Polymerase Chain Reaction:Article Title: A peptide derived from an extracellular domain selectively inhibits receptor internalization: Target sequences on insulin and insulin-like growth factor 1 receptors Article Snippet: IRp binding to IGF-1R was carried out in the presence or absence of 5 nM insulin or IGF-1. .. Deletion mutants were constructed using the PCR and standard cloning techniques , and subcloned into a Article Title: Technical Brief: Subretinal injection and electroporation into adult mouse eyes Article Snippet: .. Reporter gene plasmid The base vector was the Clone Assay:Article Title: A peptide derived from an extracellular domain selectively inhibits receptor internalization: Target sequences on insulin and insulin-like growth factor 1 receptors Article Snippet: IRp binding to IGF-1R was carried out in the presence or absence of 5 nM insulin or IGF-1. .. Deletion mutants were constructed using the PCR and standard cloning techniques , and subcloned into a Article Title: Pathogenic Natural Antibodies Recognizing Annexin IV Are Required to Develop Intestinal Ischemia-Reperfusion Injury Article Snippet: The KpnI/XhoI fragment was amplified by PCR from genomic DNA using Pfu polymerase (Novagen). .. The KpnI/XhoI fragment was subsequently cloned into Article Title: SDF-1? degrades, while gp120 upregulates BimEL: implications for the development of T cell memory Article Snippet: The N-terminus BimS construct was PCR amplified using a sense primer with BamH1 site (5′- CGGATCC ATGGCAAAGCAA) coupled with antisense splicing primer (5′-GGAAGCTTGTGGCTC) and C-terminus BimS was PCR amplified using a antisense primer with EcoR1 site (5′- CGAATTC TCAATGCATTCT) coupled with sense splicing primer (5′-CCACAAGCTTCCATGAGG). .. The BimEL , BimL and BimS were cloned into either Expressing:Article Title: A peptide derived from an extracellular domain selectively inhibits receptor internalization: Target sequences on insulin and insulin-like growth factor 1 receptors Article Snippet: IRp binding to IGF-1R was carried out in the presence or absence of 5 nM insulin or IGF-1. .. Deletion mutants were constructed using the PCR and standard cloning techniques , and subcloned into a Article Title: The functional expression of toxic genes: Lessons learned from molecular cloning of CCH1, a high-affinity Ca2+ channel Article Snippet: HEK293 cells were purchased from ATCC (CRL-1573). .. The aim was to clone CCH1 into the pcDNA3.1/CT-GFP-topo expression plasmid under the control of the Article Title: Pathogenic Natural Antibodies Recognizing Annexin IV Are Required to Develop Intestinal Ischemia-Reperfusion Injury Article Snippet: The KpnI/XhoI fragment was amplified by PCR from genomic DNA using Pfu polymerase (Novagen). .. The KpnI/XhoI fragment was subsequently cloned into Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis Article Snippet: A polyclonal antiserum was raised by immunizing a rabbit with recombinant MDC or CCR4 following previously described procedures. .. The full-length rat MDC and CCR4 were subcloned into the Article Title: c-Myc Transformation Domain Recruits the Human STAGA Complex and Requires TRRAP and GCN5 Acetylase Activity for Transcription Activation* Article Snippet: HEK293 cells were seeded on 10-cm plates in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (without antibiotics) at a density of 6 × 106 24 h before transfection. .. Cells at ~90% confluence were transfected with 10 μ g of Article Title: SDF-1? degrades, while gp120 upregulates BimEL: implications for the development of T cell memory Article Snippet: The N-terminus BimS construct was PCR amplified using a sense primer with BamH1 site (5′- CGGATCC ATGGCAAAGCAA) coupled with antisense splicing primer (5′-GGAAGCTTGTGGCTC) and C-terminus BimS was PCR amplified using a antisense primer with EcoR1 site (5′- CGAATTC TCAATGCATTCT) coupled with sense splicing primer (5′-CCACAAGCTTCCATGAGG). .. The BimEL , BimL and BimS were cloned into either Article Title: Technical Brief: Subretinal injection and electroporation into adult mouse eyes Article Snippet: .. Reporter gene plasmid The base vector was the Article Title: The human cytomegalovirus protein UL116 interacts with the viral ER resident glycoprotein UL148 and promotes the incorporation of gH/gL complexes into virions Article Snippet: The next day, the Protein G magnetic beads were washed three times in lysis buffer, and eluted by heating (50°C, 10 min) in 2×Laemmli buffer (4% SDS, 20% glycerol, 0.004% bromphenol blue, 0.125 M Tris HCl, pH 6.8) lacking reducing agent. .. To obtain starting material for IP experiments from transfected cells, 3 μg of Plasmid Preparation:Article Title: A peptide derived from an extracellular domain selectively inhibits receptor internalization: Target sequences on insulin and insulin-like growth factor 1 receptors Article Snippet: IRp binding to IGF-1R was carried out in the presence or absence of 5 nM insulin or IGF-1. .. Deletion mutants were constructed using the PCR and standard cloning techniques , and subcloned into a Article Title: The functional expression of toxic genes: Lessons learned from molecular cloning of CCH1, a high-affinity Ca2+ channel Article Snippet: HEK293 cells were purchased from ATCC (CRL-1573). .. The aim was to clone CCH1 into the pcDNA3.1/CT-GFP-topo expression plasmid under the control of the Article Title: Pathogenic Natural Antibodies Recognizing Annexin IV Are Required to Develop Intestinal Ischemia-Reperfusion Injury Article Snippet: The KpnI/XhoI fragment was amplified by PCR from genomic DNA using Pfu polymerase (Novagen). .. The KpnI/XhoI fragment was subsequently cloned into Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis Article Snippet: A polyclonal antiserum was raised by immunizing a rabbit with recombinant MDC or CCR4 following previously described procedures. .. The full-length rat MDC and CCR4 were subcloned into the Article Title: SDF-1? degrades, while gp120 upregulates BimEL: implications for the development of T cell memory Article Snippet: The N-terminus BimS construct was PCR amplified using a sense primer with BamH1 site (5′- CGGATCC ATGGCAAAGCAA) coupled with antisense splicing primer (5′-GGAAGCTTGTGGCTC) and C-terminus BimS was PCR amplified using a antisense primer with EcoR1 site (5′- CGAATTC TCAATGCATTCT) coupled with sense splicing primer (5′-CCACAAGCTTCCATGAGG). .. The BimEL , BimL and BimS were cloned into either Article Title: Technical Brief: Subretinal injection and electroporation into adult mouse eyes Article Snippet: .. Reporter gene plasmid The base vector was the Article Title: The human cytomegalovirus protein UL116 interacts with the viral ER resident glycoprotein UL148 and promotes the incorporation of gH/gL complexes into virions Article Snippet: The next day, the Protein G magnetic beads were washed three times in lysis buffer, and eluted by heating (50°C, 10 min) in 2×Laemmli buffer (4% SDS, 20% glycerol, 0.004% bromphenol blue, 0.125 M Tris HCl, pH 6.8) lacking reducing agent. .. To obtain starting material for IP experiments from transfected cells, 3 μg of Transfection:Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis Article Snippet: A polyclonal antiserum was raised by immunizing a rabbit with recombinant MDC or CCR4 following previously described procedures. .. The full-length rat MDC and CCR4 were subcloned into the Article Title: c-Myc Transformation Domain Recruits the Human STAGA Complex and Requires TRRAP and GCN5 Acetylase Activity for Transcription Activation* Article Snippet: HEK293 cells were seeded on 10-cm plates in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (without antibiotics) at a density of 6 × 106 24 h before transfection. .. Cells at ~90% confluence were transfected with 10 μ g of Article Title: The human cytomegalovirus protein UL116 interacts with the viral ER resident glycoprotein UL148 and promotes the incorporation of gH/gL complexes into virions Article Snippet: The next day, the Protein G magnetic beads were washed three times in lysis buffer, and eluted by heating (50°C, 10 min) in 2×Laemmli buffer (4% SDS, 20% glycerol, 0.004% bromphenol blue, 0.125 M Tris HCl, pH 6.8) lacking reducing agent. .. To obtain starting material for IP experiments from transfected cells, 3 μg of Electroporation:Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis Article Snippet: A polyclonal antiserum was raised by immunizing a rabbit with recombinant MDC or CCR4 following previously described procedures. .. The full-length rat MDC and CCR4 were subcloned into the Amplification:Article Title: Technical Brief: Subretinal injection and electroporation into adult mouse eyes Article Snippet: .. Reporter gene plasmid The base vector was the Incubation:Article Title: Technical Brief: Subretinal injection and electroporation into adult mouse eyes Article Snippet: .. Reporter gene plasmid The base vector was the |