strain atcc 43503 additional 5 dilutions  (ATCC)

Journal:

Article Title: Molecular Ecology of Tetracycline Resistance: Development and Validation of Primers for Detection of Tetracycline Resistance Genes Encoding Ribosomal Protection Proteins

doi: 10.1128/AEM.67.1.22-32.2001

Figure Lengend Snippet: Characteristics of bacterial strains and plasmids used in this study

Article Snippet: The cloned tet genes were maintained in E. coli by selection of plasmid antibiotic markers (ampicillin or kanamycin at a concentration of 50 μg/ml or chloramphenicol at a concentration of 20 μg/ml). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Relevant characteristics Reference or source Strains C. jejuni subsp. jejuni Tc r , tet (O) ATCC 43503 S. pyogenes A498 Tc r , tet (T) 9 C. perfringens JIR4202 Tc r , tetA (P) tetB (P) 38 Plasmids pBT-1 pJRD215 carrying a 2.5-kb Sst I fragment containing the tet (Q) gene of Tc r Em r DOT 28 pJIR667 pPR328 carrying a 1.1-kb Pst I- Eco RI internal fragment of the tetB (P) gene 20 pFD310 Carries the tet (M) gene from S. agalactiae 39 pGEM-tetW pGEM carrying a 2.4-kb PCR product with the tet (W) gene from B. fibrisolvens 2 pGEM-tetO pGEM carrying the tet (O) gene from B. fibrisolvens 2 pVP2 pUC18 carrying a 5.88-kb Cla I fragment of pK214 encompassing the tet (S) gene 31 pAT451 pUC18 carrying a 4.5-kb Cla I fragment of pIP811 with the tet (S) gene 7 pCT10 Contains the tet gene from S. lividans 1326 10 Open in a separate window Characteristics of bacterial strains and plasmids used in this study For isolation of fecal streptococci, fresh fecal samples from six pigs were resuspended in phosphate-buffered saline (pH 7.0), and dilutions were plated on MRS agar (Difco) plates.

Techniques: Plasmid Preparation

Journal:

Article Title: Molecular Ecology of Tetracycline Resistance: Development and Validation of Primers for Detection of Tetracycline Resistance Genes Encoding Ribosomal Protection Proteins

doi: 10.1128/AEM.67.1.22-32.2001

Figure Lengend Snippet: Validation of PCR primers with control templates

Article Snippet: The cloned tet genes were maintained in E. coli by selection of plasmid antibiotic markers (ampicillin or kanamycin at a concentration of 50 μg/ml or chloramphenicol at a concentration of 20 μg/ml). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Relevant characteristics Reference or source Strains C. jejuni subsp. jejuni Tc r , tet (O) ATCC 43503 S. pyogenes A498 Tc r , tet (T) 9 C. perfringens JIR4202 Tc r , tetA (P) tetB (P) 38 Plasmids pBT-1 pJRD215 carrying a 2.5-kb Sst I fragment containing the tet (Q) gene of Tc r Em r DOT 28 pJIR667 pPR328 carrying a 1.1-kb Pst I- Eco RI internal fragment of the tetB (P) gene 20 pFD310 Carries the tet (M) gene from S. agalactiae 39 pGEM-tetW pGEM carrying a 2.4-kb PCR product with the tet (W) gene from B. fibrisolvens 2 pGEM-tetO pGEM carrying the tet (O) gene from B. fibrisolvens 2 pVP2 pUC18 carrying a 5.88-kb Cla I fragment of pK214 encompassing the tet (S) gene 31 pAT451 pUC18 carrying a 4.5-kb Cla I fragment of pIP811 with the tet (S) gene 7 pCT10 Contains the tet gene from S. lividans 1326 10 Open in a separate window Characteristics of bacterial strains and plasmids used in this study For isolation of fecal streptococci, fresh fecal samples from six pigs were resuspended in phosphate-buffered saline (pH 7.0), and dilutions were plated on MRS agar (Difco) plates.

Techniques: Biomarker Discovery, Control, Amplification, Plasmid Preparation