rabbit anti 5 ht 1b receptor blocking peptide  (Alomone Labs)

Journal: The Journal of Neuroscience

Article Title: The Effect of Serotonin Receptor 5-HT1B on Lateral Inhibition between Spiny Projection Neurons in the Mouse Striatum

doi: 10.1523/JNEUROSCI.1037-20.2021

Figure Lengend Snippet: AAV5-mediated ChR2 is absent in CINs of D1/A2a-cre mice. A–E , Different section showing absence of Cre-dependent ChR2 expression from CINs. A , ChR2 expression of D1-SPNs in D1-Cre mice (arrowheads). B , Example CIN. C , Serotonin 5-HT1B receptor clustered on somata of CIN and other neurons. D , Cell nuclei stained with DAPI. Scale bar: 20 µm. E , Overlay of E–H . F–J , Magnification of rectangle from A–E . F , ChR2 expressed in the cell membrane (arrowheads) and spines (arrows) of D1-Cre SPNs. G , CINs. H , Serotonin 5-HT1B receptor. I , Cell nuclei stained with DAPI. J , Overlay showing ChAT-positive cells negative for ChR2 (arrowheads). Serotonin receptor 5-HT1B is clustered on somata of CINs and SPNs. Scale bar: 10 µm.

Article Snippet: The following antibodies were used: 5-HT1B (ASR-022, Alomone Labs, 1:200), choline acetyltransferase (AB144P, Chemicon, 1:150), and DARPP-32 (611520, BD Biosciences, 1:250).

Techniques: Expressing, Staining

Journal: The Journal of Neuroscience

Article Title: The Effect of Serotonin Receptor 5-HT1B on Lateral Inhibition between Spiny Projection Neurons in the Mouse Striatum

doi: 10.1523/JNEUROSCI.1037-20.2021

Figure Lengend Snippet: Serotonin receptor 5-HT1B agonist CP-93129 lowers the average IPSC amplitude and synaptic success rate. A , Example IPSC amplitude recording and drug treatment for D1→D2-SPN connections (D1-Cre mouse). Bath application of serotonin receptor 5-HT1B agonist CP-93129 results in silencing of IPSCs. Recording of IPSCs resumes after washout. B , Example IPSC amplitude recording and drug treatment for D2→D1-SPN connections (A2a-Cre mouse). Bath application of serotonin receptor 5-HT1B agonist CP-93129 results in reduction of IPSCs amplitude and increased failures. IPSC recording recovers after washout. C , E , G , Average IPSC amplitude change during drug treatment for D2 SPNs ( N = 12, D1-Cre mice; C ), D1-SPNs ( N = 6, A2a-Cre mice; E ) and control conditions [ N = 6: D1-Cre (3), A2a-Cre (3); G )]. Each point is an average of six IPSC events. Dotted lines represent SEM IPSC amplitude change was normalized against last 5 min of baseline. The traces on the right are examples recorded during baseline (black), drug treatment/equal period for control conditions (light gray) and washout (dark gray). The black bar represents the 2-ms light pulse. D , F , H , Average group synaptic success rate of D1→D2-SPNs ( D ), D2→D1-SPNs ( F ), and control conditions ( H ). Individual synaptic success rate was calculated as number of recorded IPSCs per minute divided by the number of maximal IPSCs per minute. D , F , H , same cells as C , E , G . Dotted lines represent SEM. All recordings were done in the presence of KA.

Article Snippet: The following antibodies were used: 5-HT1B (ASR-022, Alomone Labs, 1:200), choline acetyltransferase (AB144P, Chemicon, 1:150), and DARPP-32 (611520, BD Biosciences, 1:250).

Techniques:

Journal: The Journal of Neuroscience

Article Title: The Effect of Serotonin Receptor 5-HT1B on Lateral Inhibition between Spiny Projection Neurons in the Mouse Striatum

doi: 10.1523/JNEUROSCI.1037-20.2021

Figure Lengend Snippet: CP-93129 does not affect ChR2-IPSCs from FSIs. A , Example AP triggered in a FSI (PV-Cre mouse) by a 2-ms light pulse. Optical stimulation resulted in one (black) or two APs (gray). Blue light bar represents LED stimulus. B , Example IPSC amplitude recording and drug treatment for FSI→SPN connections (PV-Cre mouse). Bath application of serotonin receptor 5-HT1B agonist CP-93129 has no effect on IPSCs. C , Average IPSC amplitude change during CP-93129 treatment in PV-Cre mice ( N = 4). Horizontal line at 100% was added as visual guideline. Dotted lines represent SEM IPSC amplitude change was normalized against minutes 10–15 (baseline). Each point is an average of six IPSC events. D , Average group synaptic success rate of SPNs with CP-93129. Dotted lines represent SEM. All recordings were done in the presence of KA.

Article Snippet: The following antibodies were used: 5-HT1B (ASR-022, Alomone Labs, 1:200), choline acetyltransferase (AB144P, Chemicon, 1:150), and DARPP-32 (611520, BD Biosciences, 1:250).

Techniques:

Journal: The Journal of Neuroscience

Article Title: The Effect of Serotonin Receptor 5-HT1B on Lateral Inhibition between Spiny Projection Neurons in the Mouse Striatum

doi: 10.1523/JNEUROSCI.1037-20.2021

Figure Lengend Snippet: CP-93129 acts via 5-HT1B receptors located on presynaptic SPN terminals. A , Example recording of IPSC amplitudes in a D2-SPN (D1-Cre mouse) with serotonin receptor antagonist SB-216641 and agonist CP-93129. Recording started in the presence of SB-216641 and CP-93129 was added to the bath after 15 min. B , Average IPSC amplitude change in D2-SPNs (D1-Cre mice) during drug treatment ( N = 5). Horizontal line at 100% was added as visual guideline. Each point is an average of six IPSC events. Dotted lines represent SEM IPSC amplitude change was normalized against minutes 10–15 (baseline). The traces on the right are examples recorded during baseline (black), drug treatment (light gray), and washout (dark gray). The black bar represents the 2-ms light pulse. C , Average group synaptic success rate of D2-SPNs (D1-Cre mice) with SB-216641 and CP-93129. Dotted lines represent SEM. D , Before and after plot of average IPSC amplitude change of D2-SPNs (D1-Cre mice) during drug treatment. Bars represent the mean. All recordings were done in the presence of KA. ns, p > 0.05; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The following antibodies were used: 5-HT1B (ASR-022, Alomone Labs, 1:200), choline acetyltransferase (AB144P, Chemicon, 1:150), and DARPP-32 (611520, BD Biosciences, 1:250).

Techniques: