anti-somatostatin receptor type 2 antibody  (Alomone Labs)

Journal: Neuro-Oncology Advances

Article Title: Cytotoxicity on low-grade canine meningioma with the use of somatostatin analog (octreotide): An in vitro study

doi: 10.1093/noajnl/vdae111

Figure Lengend Snippet: Primary cell cultures from canine meningioma. (A) Cultured cells tend to arrange in fusiform clumps. May-Grunwald-Giemsa stain. (B) Many neoplastic cells show cytoplasmic or membrane EMA immunoreaction (Immunocytochemistry). (C) Diffuse marked cytoplasmic SSTR2 immunoreaction of the cultured neoplastic meningeal cells (Immunocytochemistry).

Article Snippet: In addition, rabbit anti-rat SSTR2 polyclonal antibody (1:500; 4°C overnight; Alomone Laboratories, Jerusalem, Israel) was used as a primary antibody.

Techniques: Cell Culture, Giemsa Stain, Membrane, Immunocytochemistry

Journal: Acta Pharmacologica Sinica

Article Title: Pharmacokinetic and pharmacodynamic evidence for developing an oral formulation of octreotide against gastric mucosal injury

doi: 10.1038/aps.2017.159

Figure Lengend Snippet: The expression levels of the main SSTRs in rat tissues, and the influence of SSTRs on the distribution of OCT in rats. (A) The relative expression levels of SSTR2, SSTR3 and SSTR5 in rat stomach, duodenum, jejunum, ileum and colon; (B) The expression of SSTR2 in rat stomach sections; (C) The expression of SSTR3 in rat stomach sections as measured by fluorescence immunoassay; (D) The expression of SSTR5 in rat stomach sections; (E) The effects of SSTR2 antagonists on the distribution of OCT. * P<0.05.

Article Snippet: After washing with phosphate-buffered solution (PBS, pH 7.4) three times, the stomach slices were blocked with 10% fetal calf serum (FCS) for 30 min. Polyclonal rabbit anti-mouse SSTR2, SSTR3 and SSTR5 primary antibodies (Alomone Lab, Jerusalem, Israel) were used at dilutions of 1:500, 1:250 and 1:250, respectively.

Techniques: Expressing, Fluorescence

Journal: Acta Pharmacologica Sinica

Article Title: Pharmacokinetic and pharmacodynamic evidence for developing an oral formulation of octreotide against gastric mucosal injury

doi: 10.1038/aps.2017.159

Figure Lengend Snippet: Protective mechanism of OCT in WIRS animals. (A) The effects of OCT on the expression of SST. * P<0.05; (B) The effects of OCT on the expression of gastrin; (C) The effects of OCT on the expression of SSTR2. * P<0.05 vs normal group. # P<0.05 vs model group; (D) A pyloric ligation-induced ulcer model; (E) The lesion areas (%) of rat stomachs. * P<0.05; (F) Gastrin levels. ** P<0.01 vs normal group. # P<0.05 vs model group; (G) Gastric acid pH levels. ** P<0.01.

Article Snippet: After washing with phosphate-buffered solution (PBS, pH 7.4) three times, the stomach slices were blocked with 10% fetal calf serum (FCS) for 30 min. Polyclonal rabbit anti-mouse SSTR2, SSTR3 and SSTR5 primary antibodies (Alomone Lab, Jerusalem, Israel) were used at dilutions of 1:500, 1:250 and 1:250, respectively.

Techniques: Expressing, Ligation