anti-somatostatin receptor type 3 antibody  (Alomone Labs)

Journal: bioRxiv

Article Title: Near-atomic structures of the BBSome reveal a novel mechanism for transition zone crossing

doi: 10.1101/2020.01.29.925610

Figure Lengend Snippet: A. Sequence analysis finds a BBSome-binding motif ([W/F/Y]R) within helix 8 in 20 of the 26 GPCRs known to localize to cilia. B. SSTR3 exit assays in the ARL6 rescue lines. Cells were treated with the SSTR3 agonist somatostatin for the indicated time points, fixed and the ciliary fluorescence of NeonGreen-tagged SSTR3 (SSTR3 NG ) was measured. Micrographs of representative individual cilia are shown on top. The ciliary fluorescence intensity of SSTR3 NG was measured in each cell line and the data are represented as violin plots.

Article Snippet: Pathway agonists were used in the following concentration: SAG (200 nM; ALX-270-426-M001; Enzo Life Sciences), somatostatin-14 (sst) (10 μM; ASR-003; Alomone Labs).

Techniques: Sequencing, Binding Assay, Fluorescence

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: Localisation of SSTRs in the cortical layers of mice primary somatosensory cortex. SSTR1 showed the most intense signal in L4 and a weaker signal in L2/3 and L5/6. SSTR2 was localised mainly in deep cortical layers (L5-6). SSTR3, SSTR4, and SSTR5 were distributed evenly throughout the cortical layers (column A and C). Column a present representative coronal brain sections through somatosensory cortex immunofluorescently labelled with antibodies specific for SSTR1-SSTR5. Column b presents the validation of antibody specificity. Coronal brain sections were treated with primary antibodies (anti-SSTR1-5) preincubated with control blocking peptides, followed by specific secondary antibodies. The addition of control peptides resulted in the disappearance of the antibody-specific signal. Scale bar 100 μm

Article Snippet: The blocking peptides used in this research to confirm antibody specificity were as follows: SSTR1 control peptide (catalogue no. ASR-001, Alomone Labs), SSTR2 control peptide (catalogue no. ab171899, Abcam), SSTR3 control peptide (catalogue no. ASR-003, Alomone Labs), peptide corresponding to the anti-SSTR4 antibody (amino acid sequence (C) QQEALQPEPGRKRIPLTRTTTF, Ontores, commercially not available), and SSTR5 control peptide (catalogue no. ASR-005, Alomone Labs).

Techniques: Blocking Assay

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: SSTRs localisation in the somatosensory cortex of mice. High magnification confocal pictures of coronal sections presenting localisation of SSTR1-5 in the mouse somatosensory cortex. a SSTR1 labelled clearly the pyramidal-like cell bodies and apical dendrites. b Lack of co-localisation of SSTR2s and PV INs in the mouse primary somatosensory cortex. Yellow lines represent two orthogonal sections of a z-series showing the distribution of green and red fluorescence in the tissue. c SSTR3 immunoreactivity was found in cell bodies and neuropil. d, e Immunofluorescent signal for SSTR4 and SSTR5 was found mainly in the neuropil and much less visible in cell bodies. Scale bar for all pictures: 10 μm

Article Snippet: The blocking peptides used in this research to confirm antibody specificity were as follows: SSTR1 control peptide (catalogue no. ASR-001, Alomone Labs), SSTR2 control peptide (catalogue no. ab171899, Abcam), SSTR3 control peptide (catalogue no. ASR-003, Alomone Labs), peptide corresponding to the anti-SSTR4 antibody (amino acid sequence (C) QQEALQPEPGRKRIPLTRTTTF, Ontores, commercially not available), and SSTR5 control peptide (catalogue no. ASR-005, Alomone Labs).

Techniques: Fluorescence

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: Two types of SSTRs distribution in the mouse barrel field. Tangential brain sections through L4 of the mouse barrel cortex. Type A distribution is characterised by a high concentration of immunoreactivity in the barrel walls (sides + septa). This type is represented by SSTR1 and SSTR2. Type B distribution is characterised by a homogeneous intensity of immunoreactivity in the barrel hollows. This type is represented by SSTR3, SSTR4, and SSTR5. Scale bar: 100 μm. Nuclear DAPI staining is visible in blue

Article Snippet: The blocking peptides used in this research to confirm antibody specificity were as follows: SSTR1 control peptide (catalogue no. ASR-001, Alomone Labs), SSTR2 control peptide (catalogue no. ab171899, Abcam), SSTR3 control peptide (catalogue no. ASR-003, Alomone Labs), peptide corresponding to the anti-SSTR4 antibody (amino acid sequence (C) QQEALQPEPGRKRIPLTRTTTF, Ontores, commercially not available), and SSTR5 control peptide (catalogue no. ASR-005, Alomone Labs).

Techniques: Concentration Assay, Staining

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: Localisation of SSTRs in the cortical layers of mice primary somatosensory cortex. SSTR1 showed the most intense signal in L4 and a weaker signal in L2/3 and L5/6. SSTR2 was localised mainly in deep cortical layers (L5-6). SSTR3, SSTR4, and SSTR5 were distributed evenly throughout the cortical layers (column A and C). Column a present representative coronal brain sections through somatosensory cortex immunofluorescently labelled with antibodies specific for SSTR1-SSTR5. Column b presents the validation of antibody specificity. Coronal brain sections were treated with primary antibodies (anti-SSTR1-5) preincubated with control blocking peptides, followed by specific secondary antibodies. The addition of control peptides resulted in the disappearance of the antibody-specific signal. Scale bar 100 μm

Article Snippet: The primary antibodies used in this study were as follows: rabbit anti-SSTR1 polyclonal antibody (1:400, catalogue no. ASR-001, Alomone Labs), rabbit anti-SSTR2 monoclonal antibody (1:2000, catalogue no. ab134152, Abcam), rabbit anti-SSTR3 polyclonal antibody (1:300, catalogue no. ASR-003, Alomone Labs), rabbit anti-SSTR4 polyclonal antibody (1:1000, catalogue no. ab28578, Abcam), and rabbit anti-SSTR5 polyclonal antibody (1:1000, catalogue no. ASR-005, Alomone Labs).

Techniques: Blocking Assay

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: SSTRs localisation in the somatosensory cortex of mice. High magnification confocal pictures of coronal sections presenting localisation of SSTR1-5 in the mouse somatosensory cortex. a SSTR1 labelled clearly the pyramidal-like cell bodies and apical dendrites. b Lack of co-localisation of SSTR2s and PV INs in the mouse primary somatosensory cortex. Yellow lines represent two orthogonal sections of a z-series showing the distribution of green and red fluorescence in the tissue. c SSTR3 immunoreactivity was found in cell bodies and neuropil. d, e Immunofluorescent signal for SSTR4 and SSTR5 was found mainly in the neuropil and much less visible in cell bodies. Scale bar for all pictures: 10 μm

Article Snippet: The primary antibodies used in this study were as follows: rabbit anti-SSTR1 polyclonal antibody (1:400, catalogue no. ASR-001, Alomone Labs), rabbit anti-SSTR2 monoclonal antibody (1:2000, catalogue no. ab134152, Abcam), rabbit anti-SSTR3 polyclonal antibody (1:300, catalogue no. ASR-003, Alomone Labs), rabbit anti-SSTR4 polyclonal antibody (1:1000, catalogue no. ab28578, Abcam), and rabbit anti-SSTR5 polyclonal antibody (1:1000, catalogue no. ASR-005, Alomone Labs).

Techniques: Fluorescence

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: Two types of SSTRs distribution in the mouse barrel field. Tangential brain sections through L4 of the mouse barrel cortex. Type A distribution is characterised by a high concentration of immunoreactivity in the barrel walls (sides + septa). This type is represented by SSTR1 and SSTR2. Type B distribution is characterised by a homogeneous intensity of immunoreactivity in the barrel hollows. This type is represented by SSTR3, SSTR4, and SSTR5. Scale bar: 100 μm. Nuclear DAPI staining is visible in blue

Article Snippet: The primary antibodies used in this study were as follows: rabbit anti-SSTR1 polyclonal antibody (1:400, catalogue no. ASR-001, Alomone Labs), rabbit anti-SSTR2 monoclonal antibody (1:2000, catalogue no. ab134152, Abcam), rabbit anti-SSTR3 polyclonal antibody (1:300, catalogue no. ASR-003, Alomone Labs), rabbit anti-SSTR4 polyclonal antibody (1:1000, catalogue no. ab28578, Abcam), and rabbit anti-SSTR5 polyclonal antibody (1:1000, catalogue no. ASR-005, Alomone Labs).

Techniques: Concentration Assay, Staining

Journal: Acta Pharmacologica Sinica

Article Title: Pharmacokinetic and pharmacodynamic evidence for developing an oral formulation of octreotide against gastric mucosal injury

doi: 10.1038/aps.2017.159

Figure Lengend Snippet: The expression levels of the main SSTRs in rat tissues, and the influence of SSTRs on the distribution of OCT in rats. (A) The relative expression levels of SSTR2, SSTR3 and SSTR5 in rat stomach, duodenum, jejunum, ileum and colon; (B) The expression of SSTR2 in rat stomach sections; (C) The expression of SSTR3 in rat stomach sections as measured by fluorescence immunoassay; (D) The expression of SSTR5 in rat stomach sections; (E) The effects of SSTR2 antagonists on the distribution of OCT. * P<0.05.

Article Snippet: After washing with phosphate-buffered solution (PBS, pH 7.4) three times, the stomach slices were blocked with 10% fetal calf serum (FCS) for 30 min. Polyclonal rabbit anti-mouse SSTR2, SSTR3 and SSTR5 primary antibodies (Alomone Lab, Jerusalem, Israel) were used at dilutions of 1:500, 1:250 and 1:250, respectively.

Techniques: Expressing, Fluorescence