sstr2  (Alomone Labs)

Journal: Veterinary Medicine and Science

Article Title: Expression of somatostatin receptors in canine and feline meningioma

doi: 10.1002/vms3.1537

Figure Lengend Snippet: Primers that were used in real‐time (RT)‐qPCR.

Article Snippet: The antibodies against SSTR1 (#ASR‐001) and SSTR2 (#ASR‐002) (Alomone Laboratories) were diluted in PBS at a dilution of 1:200 and 1:500, respectively, and incubated at 4°C overnight.

Techniques: Sequencing, Amplification

Journal: Veterinary Medicine and Science

Article Title: Expression of somatostatin receptors in canine and feline meningioma

doi: 10.1002/vms3.1537

Figure Lengend Snippet: Examples of immunohistochemistry (IHC) scoring for SSTR2. IHC staining examples for SSTR2. (A) serves as the negative control without the secondary antibody addition. (B) represents a feline meningioma with c staining. Letter (C) denotes a feline meningioma with m/c/n staining. (D) signifies a canine meningioma with m/c/n staining. c, cytoplasmic staining; m, membranous staining; n, nuclear staining.

Article Snippet: The antibodies against SSTR1 (#ASR‐001) and SSTR2 (#ASR‐002) (Alomone Laboratories) were diluted in PBS at a dilution of 1:200 and 1:500, respectively, and incubated at 4°C overnight.

Techniques: Immunohistochemistry, Negative Control, Staining

Journal: Veterinary Medicine and Science

Article Title: Expression of somatostatin receptors in canine and feline meningioma

doi: 10.1002/vms3.1537

Figure Lengend Snippet: Real‐time (RT)‐qPCR results.

Article Snippet: The antibodies against SSTR1 (#ASR‐001) and SSTR2 (#ASR‐002) (Alomone Laboratories) were diluted in PBS at a dilution of 1:200 and 1:500, respectively, and incubated at 4°C overnight.

Techniques:

Journal: Veterinary Medicine and Science

Article Title: Expression of somatostatin receptors in canine and feline meningioma

doi: 10.1002/vms3.1537

Figure Lengend Snippet: Western blotting (WB). Specificity of the antibodies (SSTR1 [around 60 kDa] and SSTR2 [around 50 kDa]) for canine and feline tissue was assessed using WB. (A) WB for SSTR1; (B) WB for SSTR2. Lane 1, mouse brain; lane 2, dog brain; lane 3, cat brain. Pre‐blocking of the antibodies with the respective peptides before the detection process resulted in a significant reduction or decrease in the specific bands (lanes 1a–3a). Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) (42 kDa) was used as the loading control. MW, molecular weight marker.

Article Snippet: The antibodies against SSTR1 (#ASR‐001) and SSTR2 (#ASR‐002) (Alomone Laboratories) were diluted in PBS at a dilution of 1:200 and 1:500, respectively, and incubated at 4°C overnight.

Techniques: Western Blot, Blocking Assay, Control, Molecular Weight, Marker

Journal: Veterinary Medicine and Science

Article Title: Expression of somatostatin receptors in canine and feline meningioma

doi: 10.1002/vms3.1537

Figure Lengend Snippet: Immunohistochemistry (IHC) results.

Article Snippet: The antibodies against SSTR1 (#ASR‐001) and SSTR2 (#ASR‐002) (Alomone Laboratories) were diluted in PBS at a dilution of 1:200 and 1:500, respectively, and incubated at 4°C overnight.

Techniques: Immunohistochemistry, Staining

Journal: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

Article Title: Fos and pERK immunoreactivity in spinal cord slices: Comparative analysis of in vitro models for testing putative antinociceptive molecules.

doi: 10.1016/j.aanat.2013.11.005

Figure Lengend Snippet: Fig. 3. Immunohistochemical localization of SSTR2a at the light and electron microscopic level. (A) SSTR2a immunoreactive (IR) nociceptive PAFs in lamina IIi of the spinal DH (red). (B) SST-28 IR peptidergic nociceptive PAFs in lamina I–IIo (green). (C) SSTR2a and SST-28 are distributed in two different populations of nociceptive PAFs in lamina I–II (merge). (D) SSTR2a IR nociceptive PAFs in lamina IIi of the spinal DH (red). (E) IB4 IR non-peptidergic nociceptive PAFs in lamina IIi (green). (F) SSTR2a and IB4 are co-expressed in nociceptive non-peptidergic PAFs of lamina IIi (merge). (G) SSTR2a IR nociceptive PAFs in lamina IIi of the spinal cord (red). (H) TRPV1 IR nociceptive fibers in lamina I–IIo (green). (I) SSTR2a and TRPV1 are co-expressed in a population of PAFs distributed at the border between lamina IIo and lamina IIi (merge). (J) Post-embedding immunogold labeling for SST-28 + CGRP in spinal lamina II. SST-28 (10 nm) and CGRP (20 nm) are selectively localized in DCVs of a peptidergic type Ib glomerular terminal (GIb). The SST-28 + CGRP IR GIb contacts three unlabeled dendrites (d1–d3) and a peripheral axonal bouton (v2; see Ribeiro da Silva, 2004). (K) Combined pre-embedding FluoroNanogold SSTR2a and post-embedding IB4 IRs in non-peptidergic type Ia glomeruli (GIa) of lamina II. SSTR2a receptors have been localized by gold intensified, irregular particles (25–30 nm; arrowhead in the insert), while IB4 immunostaining was characterized by gold particles of regular round shape and constant size (20 nm, arrow in the insert). The SSTR2a + IB4-IR GIa terminal is surrounded by several unlabeled dendrites (d1–d6) and two vesicle-containing dendrite (v1; see Ribeiro da Silva, 2004). Scale bars: A–I: 250 m; J–K: 500 nm.

Article Snippet: The following primary antibodies have een used in this study: goat-anti-somatostatin receptor type 2a SSTR2a; 1:100 – LM and EM; Santa Cruz Biotechnology), rabbit nti-somatostatin (SST-28; 1:2000 – LM, 1:100 – EM; Merighi et al., 989), rabbit anti-CGRP (1:1000 – LM, 1:100 – EM; Merighi et al., 991), rabbit anti-TRPV1 (1:1000 – LM; Alomone Labs, Jerusalem, srael).

Techniques: Immunohistochemical staining, Labeling, Immunostaining