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The results of <t>IHC</t> analysis of <t>AREG</t> protein in RCC tumours. The plots show mean optical density (OD = log10(max_intensity/mean_intensity)) obtained from the 5 randomly picked microscopic fields per each tissue sample. The analysis was performed on tissue samples from 16 patients. A . AREG expression in matched-paired areas of tumour (n=16) and stroma (16). B . AREG expression in ccRCC tumours of lower grade (G1, n=6) when compared with tumours of higher grade (G3+G4, n=10). C . AREG expression in ccRCC tumours classified as TNM Stage 1 (n=7) and Stage 4 (n=9). Statistical analysis was performed using t-test. * p < 0.05. **** p < 0.0001. D . AREG expression in tumours correlates with age of patients. See also Supplementary Table S4 (patients clinical data) and Supplementary Figure S4 (microscopic images of IHC).
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The results of <t>IHC</t> analysis of <t>AREG</t> protein in RCC tumours. The plots show mean optical density (OD = log10(max_intensity/mean_intensity)) obtained from the 5 randomly picked microscopic fields per each tissue sample. The analysis was performed on tissue samples from 16 patients. A . AREG expression in matched-paired areas of tumour (n=16) and stroma (16). B . AREG expression in ccRCC tumours of lower grade (G1, n=6) when compared with tumours of higher grade (G3+G4, n=10). C . AREG expression in ccRCC tumours classified as TNM Stage 1 (n=7) and Stage 4 (n=9). Statistical analysis was performed using t-test. * p < 0.05. **** p < 0.0001. D . AREG expression in tumours correlates with age of patients. See also Supplementary Table S4 (patients clinical data) and Supplementary Figure S4 (microscopic images of IHC).
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The results of <t>IHC</t> analysis of <t>AREG</t> protein in RCC tumours. The plots show mean optical density (OD = log10(max_intensity/mean_intensity)) obtained from the 5 randomly picked microscopic fields per each tissue sample. The analysis was performed on tissue samples from 16 patients. A . AREG expression in matched-paired areas of tumour (n=16) and stroma (16). B . AREG expression in ccRCC tumours of lower grade (G1, n=6) when compared with tumours of higher grade (G3+G4, n=10). C . AREG expression in ccRCC tumours classified as TNM Stage 1 (n=7) and Stage 4 (n=9). Statistical analysis was performed using t-test. * p < 0.05. **** p < 0.0001. D . AREG expression in tumours correlates with age of patients. See also Supplementary Table S4 (patients clinical data) and Supplementary Figure S4 (microscopic images of IHC).
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The results of <t>IHC</t> analysis of <t>AREG</t> protein in RCC tumours. The plots show mean optical density (OD = log10(max_intensity/mean_intensity)) obtained from the 5 randomly picked microscopic fields per each tissue sample. The analysis was performed on tissue samples from 16 patients. A . AREG expression in matched-paired areas of tumour (n=16) and stroma (16). B . AREG expression in ccRCC tumours of lower grade (G1, n=6) when compared with tumours of higher grade (G3+G4, n=10). C . AREG expression in ccRCC tumours classified as TNM Stage 1 (n=7) and Stage 4 (n=9). Statistical analysis was performed using t-test. * p < 0.05. **** p < 0.0001. D . AREG expression in tumours correlates with age of patients. See also Supplementary Table S4 (patients clinical data) and Supplementary Figure S4 (microscopic images of IHC).
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The results of <t>IHC</t> analysis of <t>AREG</t> protein in RCC tumours. The plots show mean optical density (OD = log10(max_intensity/mean_intensity)) obtained from the 5 randomly picked microscopic fields per each tissue sample. The analysis was performed on tissue samples from 16 patients. A . AREG expression in matched-paired areas of tumour (n=16) and stroma (16). B . AREG expression in ccRCC tumours of lower grade (G1, n=6) when compared with tumours of higher grade (G3+G4, n=10). C . AREG expression in ccRCC tumours classified as TNM Stage 1 (n=7) and Stage 4 (n=9). Statistical analysis was performed using t-test. * p < 0.05. **** p < 0.0001. D . AREG expression in tumours correlates with age of patients. See also Supplementary Table S4 (patients clinical data) and Supplementary Figure S4 (microscopic images of IHC).
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The results of IHC analysis of AREG protein in RCC tumours. The plots show mean optical density (OD = log10(max_intensity/mean_intensity)) obtained from the 5 randomly picked microscopic fields per each tissue sample. The analysis was performed on tissue samples from 16 patients. A . AREG expression in matched-paired areas of tumour (n=16) and stroma (16). B . AREG expression in ccRCC tumours of lower grade (G1, n=6) when compared with tumours of higher grade (G3+G4, n=10). C . AREG expression in ccRCC tumours classified as TNM Stage 1 (n=7) and Stage 4 (n=9). Statistical analysis was performed using t-test. * p < 0.05. **** p < 0.0001. D . AREG expression in tumours correlates with age of patients. See also Supplementary Table S4 (patients clinical data) and Supplementary Figure S4 (microscopic images of IHC).

Journal: bioRxiv

Article Title: Renal cancer cell-derived amphiregulin recruits mesenchymal stromal cells, induces their glycolytic switch, and promotes tumour growth

doi: 10.1101/2025.10.14.681141

Figure Lengend Snippet: The results of IHC analysis of AREG protein in RCC tumours. The plots show mean optical density (OD = log10(max_intensity/mean_intensity)) obtained from the 5 randomly picked microscopic fields per each tissue sample. The analysis was performed on tissue samples from 16 patients. A . AREG expression in matched-paired areas of tumour (n=16) and stroma (16). B . AREG expression in ccRCC tumours of lower grade (G1, n=6) when compared with tumours of higher grade (G3+G4, n=10). C . AREG expression in ccRCC tumours classified as TNM Stage 1 (n=7) and Stage 4 (n=9). Statistical analysis was performed using t-test. * p < 0.05. **** p < 0.0001. D . AREG expression in tumours correlates with age of patients. See also Supplementary Table S4 (patients clinical data) and Supplementary Figure S4 (microscopic images of IHC).

Article Snippet: Immunohistochemical analysis was done using archived FFPE tissue specimens of clear cell renal cell carcinoma (ccRCC) with the approval of the local Bioethics Committee (approval no. 21/2025 and 1/2025) using IHCeasy AREG Ready-To-Use IHC Kit (Cat No. KHC0808; Proteintech Group, Rosemont, IL, USA) in accordance with the manufacturer’s protocol.

Techniques: Expressing