Structured Review

Phenomenex aqua c18
Aqua C18, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/aqua c18/product/Phenomenex
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
aqua c18 - by Bioz Stars, 2021-09
86/100 stars

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Activity Assay:

Article Title: Grifolin, neogrifolin and confluentin from the terricolous polypore Albatrellus flettii suppress KRAS expression in human colon cancer cells
Article Snippet: .. Fractions 30–36, which exhibited anti-cell viability activity, were first subjected to an analytical HPLC reverse phase column Phenomenex Aqua® C18. ..

High Performance Liquid Chromatography:

Article Title: Grifolin, neogrifolin and confluentin from the terricolous polypore Albatrellus flettii suppress KRAS expression in human colon cancer cells
Article Snippet: .. Fractions 30–36, which exhibited anti-cell viability activity, were first subjected to an analytical HPLC reverse phase column Phenomenex Aqua® C18. ..

Article Title: Measurement of Antioxidant Activity of Wine Catechins, Procyanidins, Anthocyanins and Pyranoanthocyanins
Article Snippet: .. The reaction mixture was injected directly into the HPLC column (Aqua® C18, 5 μm, 150 × 4.6 mm, Phenomenex), the mobile phase, in isocratic conditions, consisted of 90% 40 mM HCOOH and 10% CH3 CN (v/v), at a flow rate of 1 ml/min. ..

Article Title: Early‐Stage Incorporation Strategy for Regioselective Labeling of Peptides using the 2‐Cyanobenzothiazole/1,2‐Aminothiol Bioorthogonal Click Reaction
Article Snippet: .. The analyses of FITC‐labeled CBT or peptides were performed by HPLC on an analytical RP‐C18 column (Aqua®, Phenomenex, 5 μm, 4.6×250 mm) with a gradient elution of acetonitrile (ACN; 10 % to 90 % in H2 O, containing 0.1 % TFA) at a flow rate of 1 mL min−1 over 30 min. ..

Mass Spectrometry:

Article Title: Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation
Article Snippet: .. For mass spectrometry, we used LC-MS chromasolv solvents (water, acetonitrile–noted MeCN), ammomium bicarbonate, iodoacetamide (I6125), and dithiothreitol (DTT, 43815) from Sigma-Aldrich (St. Louis, MO, USA), formic acid, eluent additive for LC-MS (56302) from Fluka (Honeywell, Romania), sequencing grade Trypsin, Chymotrypsin, and rLysC from Promega, Madison, WI, USA, C18 bulk (Aqua 5 µm C18 200Å bulk, Phenomenex, Torrance, CA, USA), C8 material (3M™ Empore™ C8 disks), Titansphere™ Phos-TiO Kit, 1mg TiO2 / 10µL tip (5010-21309), GL-Tip SDB (7820-11200), and GL-Tip GC (7820-11201) all from GL Sciences (Tokyo, Japan) and Amicon Ultra-0.5mL Centrifugal Filter Unit with Ultracel-30 membrane, from Millipore (USA). ..

Liquid Chromatography with Mass Spectroscopy:

Article Title: Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation
Article Snippet: .. For mass spectrometry, we used LC-MS chromasolv solvents (water, acetonitrile–noted MeCN), ammomium bicarbonate, iodoacetamide (I6125), and dithiothreitol (DTT, 43815) from Sigma-Aldrich (St. Louis, MO, USA), formic acid, eluent additive for LC-MS (56302) from Fluka (Honeywell, Romania), sequencing grade Trypsin, Chymotrypsin, and rLysC from Promega, Madison, WI, USA, C18 bulk (Aqua 5 µm C18 200Å bulk, Phenomenex, Torrance, CA, USA), C8 material (3M™ Empore™ C8 disks), Titansphere™ Phos-TiO Kit, 1mg TiO2 / 10µL tip (5010-21309), GL-Tip SDB (7820-11200), and GL-Tip GC (7820-11201) all from GL Sciences (Tokyo, Japan) and Amicon Ultra-0.5mL Centrifugal Filter Unit with Ultracel-30 membrane, from Millipore (USA). ..

Sequencing:

Article Title: Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation
Article Snippet: .. For mass spectrometry, we used LC-MS chromasolv solvents (water, acetonitrile–noted MeCN), ammomium bicarbonate, iodoacetamide (I6125), and dithiothreitol (DTT, 43815) from Sigma-Aldrich (St. Louis, MO, USA), formic acid, eluent additive for LC-MS (56302) from Fluka (Honeywell, Romania), sequencing grade Trypsin, Chymotrypsin, and rLysC from Promega, Madison, WI, USA, C18 bulk (Aqua 5 µm C18 200Å bulk, Phenomenex, Torrance, CA, USA), C8 material (3M™ Empore™ C8 disks), Titansphere™ Phos-TiO Kit, 1mg TiO2 / 10µL tip (5010-21309), GL-Tip SDB (7820-11200), and GL-Tip GC (7820-11201) all from GL Sciences (Tokyo, Japan) and Amicon Ultra-0.5mL Centrifugal Filter Unit with Ultracel-30 membrane, from Millipore (USA). ..

Injection:

Article Title: Measurement of Antioxidant Activity of Wine Catechins, Procyanidins, Anthocyanins and Pyranoanthocyanins
Article Snippet: .. The reaction mixture was injected directly into the HPLC column (Aqua® C18, 5 μm, 150 × 4.6 mm, Phenomenex), the mobile phase, in isocratic conditions, consisted of 90% 40 mM HCOOH and 10% CH3 CN (v/v), at a flow rate of 1 ml/min. ..

Flow Cytometry:

Article Title: Measurement of Antioxidant Activity of Wine Catechins, Procyanidins, Anthocyanins and Pyranoanthocyanins
Article Snippet: .. The reaction mixture was injected directly into the HPLC column (Aqua® C18, 5 μm, 150 × 4.6 mm, Phenomenex), the mobile phase, in isocratic conditions, consisted of 90% 40 mM HCOOH and 10% CH3 CN (v/v), at a flow rate of 1 ml/min. ..

Article Title: Early‐Stage Incorporation Strategy for Regioselective Labeling of Peptides using the 2‐Cyanobenzothiazole/1,2‐Aminothiol Bioorthogonal Click Reaction
Article Snippet: .. The analyses of FITC‐labeled CBT or peptides were performed by HPLC on an analytical RP‐C18 column (Aqua®, Phenomenex, 5 μm, 4.6×250 mm) with a gradient elution of acetonitrile (ACN; 10 % to 90 % in H2 O, containing 0.1 % TFA) at a flow rate of 1 mL min−1 over 30 min. ..

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  • 86
    Phenomenex semi preparative hplc
    Chromatogramm of the <t>HPLC-DAD</t> analysis of the investigated dichloromethane (DCM) extract of the roots of Doronicum austriacum (2.5 mg/mL MeOH) at 205 nm. Analytical conditions: stationary phase: <t>Phenomenex</t> Synergi Max-RP 80 Å, 4 µm (4.6 mm × 150 mm); temperature: 35 °C; mobile phase: A = water + 0.025% TFA, B = acetonitrile; flow rate: 1.00 mL/min; detection: 205 nm; injection volume: 10 µL; solvent composition during analysis: 0′: 75% A; 10′: 60% A; 30′: 30% A; 35′: 2% A; 45′: stop; post time: 10′.
    Semi Preparative Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semi preparative hplc/product/Phenomenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    semi preparative hplc - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    86
    Phenomenex phenomenex aqua c18
    HPLC-DAD chromatogram of methanolic extract of the aerial parts of D. diapensifolia . Analysis condition: stationary phase: <t>Phenomenex</t> Synergi Max-RP <t>C18</t> 4 µm, 150 × 4.6 mm; mobile Phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 98%; 35 min: B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 2 mg/mL, 10 µL; detection = 254 nm.
    Phenomenex Aqua C18, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenomenex aqua c18/product/Phenomenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenomenex aqua c18 - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    86
    Phenomenex hplc column
    HPLC-DAD chromatogram of methanolic extract of the aerial parts of D. diapensifolia . Analysis condition: stationary phase: <t>Phenomenex</t> Synergi Max-RP <t>C18</t> 4 µm, 150 × 4.6 mm; mobile Phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 98%; 35 min: B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 2 mg/mL, 10 µL; detection = 254 nm.
    Hplc Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc column/product/Phenomenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc column - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    86
    Phenomenex c18 aqua resin
    HPLC-DAD chromatogram of methanolic extract of the aerial parts of D. diapensifolia . Analysis condition: stationary phase: <t>Phenomenex</t> Synergi Max-RP <t>C18</t> 4 µm, 150 × 4.6 mm; mobile Phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 98%; 35 min: B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 2 mg/mL, 10 µL; detection = 254 nm.
    C18 Aqua Resin, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 aqua resin/product/Phenomenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c18 aqua resin - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

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    Chromatogramm of the HPLC-DAD analysis of the investigated dichloromethane (DCM) extract of the roots of Doronicum austriacum (2.5 mg/mL MeOH) at 205 nm. Analytical conditions: stationary phase: Phenomenex Synergi Max-RP 80 Å, 4 µm (4.6 mm × 150 mm); temperature: 35 °C; mobile phase: A = water + 0.025% TFA, B = acetonitrile; flow rate: 1.00 mL/min; detection: 205 nm; injection volume: 10 µL; solvent composition during analysis: 0′: 75% A; 10′: 60% A; 30′: 30% A; 35′: 2% A; 45′: stop; post time: 10′.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Anti-Inflammatory and Anti-Oxidant Potential of the Root Extract and Constituents of Doronicum austriacum

    doi: 10.3390/molecules22061003

    Figure Lengend Snippet: Chromatogramm of the HPLC-DAD analysis of the investigated dichloromethane (DCM) extract of the roots of Doronicum austriacum (2.5 mg/mL MeOH) at 205 nm. Analytical conditions: stationary phase: Phenomenex Synergi Max-RP 80 Å, 4 µm (4.6 mm × 150 mm); temperature: 35 °C; mobile phase: A = water + 0.025% TFA, B = acetonitrile; flow rate: 1.00 mL/min; detection: 205 nm; injection volume: 10 µL; solvent composition during analysis: 0′: 75% A; 10′: 60% A; 30′: 30% A; 35′: 2% A; 45′: stop; post time: 10′.

    Article Snippet: A fraction eluted with 300–980 mL (53.89 mg) was further purified by semi-preparative HPLC (stationary phase: Phenomenex Aqua C18 column (5 µm; 250 × 10 mm); mobile phase: solvent A: water solvent B: acetonitrile, gradient elution: 0 min: 45% A; 16 min: 2% A; 21 min: 45% A; 30 min: stop; flow 2.00 mL/min; 40 °C; 150 µL injection volume of a solution of 18.00 mg/mL methanol).

    Techniques: High Performance Liquid Chromatography, Flow Cytometry, Injection

    HPLC-DAD chromatogram of methanolic extract of the aerial parts of D. diapensifolia . Analysis condition: stationary phase: Phenomenex Synergi Max-RP C18 4 µm, 150 × 4.6 mm; mobile Phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 98%; 35 min: B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 2 mg/mL, 10 µL; detection = 254 nm.

    Journal: Biomolecules

    Article Title: Unusual Secondary Metabolites of the Aerial Parts of Dionysia diapensifolia Bioss. (Primulaceae) and Their Anti-Inflammatory Activity

    doi: 10.3390/biom10030438

    Figure Lengend Snippet: HPLC-DAD chromatogram of methanolic extract of the aerial parts of D. diapensifolia . Analysis condition: stationary phase: Phenomenex Synergi Max-RP C18 4 µm, 150 × 4.6 mm; mobile Phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 98%; 35 min: B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 2 mg/mL, 10 µL; detection = 254 nm.

    Article Snippet: LC- and LC-ESI-MS parameters (general method): stationary phase: Phenomenex Aqua C18 5 µm, 250 × 4.6 mm; mobile phase: A = H2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 50%; 40 min: B = 98%; 50 min B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 2–8 mg/mL, 5–15 µL in ACN, THF or MeOH.

    Techniques: High Performance Liquid Chromatography, Concentration Assay, Injection

    Chromatogram of the HPLC-DAD analysis of the diethyl ether subfraction ( A ) and an enlarged view ( B ) at λ 254 nm obtained from the methanolic extract of the aerial parts of D. diapensifolia . Analysis conditions: stationary phase: Phenomenex Aqua C18 5 µm, 250 × 4.6 mm; mobile phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 50%; 40 min: B = 98%; 50 min B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 8 mg/mL, 10 µL.

    Journal: Biomolecules

    Article Title: Unusual Secondary Metabolites of the Aerial Parts of Dionysia diapensifolia Bioss. (Primulaceae) and Their Anti-Inflammatory Activity

    doi: 10.3390/biom10030438

    Figure Lengend Snippet: Chromatogram of the HPLC-DAD analysis of the diethyl ether subfraction ( A ) and an enlarged view ( B ) at λ 254 nm obtained from the methanolic extract of the aerial parts of D. diapensifolia . Analysis conditions: stationary phase: Phenomenex Aqua C18 5 µm, 250 × 4.6 mm; mobile phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 50%; 40 min: B = 98%; 50 min B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 8 mg/mL, 10 µL.

    Article Snippet: LC- and LC-ESI-MS parameters (general method): stationary phase: Phenomenex Aqua C18 5 µm, 250 × 4.6 mm; mobile phase: A = H2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 50%; 40 min: B = 98%; 50 min B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 2–8 mg/mL, 5–15 µL in ACN, THF or MeOH.

    Techniques: High Performance Liquid Chromatography, Concentration Assay, Injection