rabbit antip2x3  (Alomone Labs)

Journal: Purinergic Signalling

Article Title: P2X3 receptor expression by HEK cells conditions their survival

doi: 10.1007/s11302-011-9285-0

Figure Lengend Snippet: P2X3 expression was time-dependent and enhanced by A-317491 antagonist. A Example of immunofluorescence microscopy photographs of HEK cells transfected with P2X3 (red) and GFP (green) analyzed at different times (24, 48, and 72 h) after transfection using anti-P2X3 antibodies. Nuclei are counterstained with DAPI (blue). Histograms (right) quantify the effect. Bar: 50 μm. Histograms quantify the effect, expressed as immunoreactive cells over cell nuclei. B Example of immunofluorescence experiments performed after 48 h from P2X3 transfection, in control and in the presence of P2X3 receptors antagonist A-317491 (10 μM, added 24 h before analysis). Histograms quantify the effect. Note how A-317491 protected the cell loss (35% increase; n = 2; p < 0.05). C Example of Western blot of P2X3-transfected cell extracts from control and from A-317491-treated cells, probed with anti-P2X3 receptors antibodies to visualize total P2X3 protein content. α-Tubulin was used as gel loading control. Histograms quantify the effect, expressed in optical density normalized over total tubulin content

Article Snippet: For immunoblotting anti-P2X3 (1:300; Alomone, Jerusalem, Israel) and anti-Actin antibodies (1:1000, Sigma) were used.

Techniques: Expressing, Immunofluorescence, Microscopy, Transfection, Western Blot

Journal: Purinergic Signalling

Article Title: P2X3 receptor expression by HEK cells conditions their survival

doi: 10.1007/s11302-011-9285-0

Figure Lengend Snippet: Expression of P2X3 mutants was controlled by P2X3 Y393A amino acid site. A Example of immunofluorescence microscopy photographs of HEK cells transfected with P2X3 Y393A (red) in control and after A-319471 application, using anti-P2X3 antibodies. Nuclei are counterstained with DAPI (blue). Histograms (right) quantify the effect. Bar: 50 μm. Histograms quantify the effect. B Example of Western blot of P2X3 Y393A-transfected cell extracts from control and from A-317491-treated cells, probed with anti-P2X3 receptors antibodies to visualize total protein content. α-Tubulin was used as gel loading control. Histograms quantify the effect, expressed in optical density over total tubulin content. Note how P2X3 receptors antagonist increases P2X3-Y393A protein recovery. n = 3; p < 0.05

Article Snippet: For immunoblotting anti-P2X3 (1:300; Alomone, Jerusalem, Israel) and anti-Actin antibodies (1:1000, Sigma) were used.

Techniques: Expressing, Immunofluorescence, Microscopy, Transfection, Western Blot

Journal: Purinergic Signalling

Article Title: P2X3 receptor expression by HEK cells conditions their survival

doi: 10.1007/s11302-011-9285-0

Figure Lengend Snippet: P2X3 expression in HEK cells was associated to caspase 3 activation. Example of immunofluorescence microscopy photographs performed with antibodies against P2X3 (green) and anti-active caspase 3 (red), as one of the hallmark of ongoing apoptosis. Control GFP-expressing cell are shown in green and were not associated to caspase activation (first upper row). Bar: 50 μm. Histograms quantify the effect, expressed as caspase-positive cells over a total of P2X3 immunopositive cells. Note how antagonist A-317491 protects from caspase activation. n = 3; p < 0.05

Article Snippet: For immunoblotting anti-P2X3 (1:300; Alomone, Jerusalem, Israel) and anti-Actin antibodies (1:1000, Sigma) were used.

Techniques: Expressing, Activation Assay, Immunofluorescence, Microscopy

Journal: Purinergic Signalling

Article Title: P2X3 receptor expression by HEK cells conditions their survival

doi: 10.1007/s11302-011-9285-0

Figure Lengend Snippet: Expression of P2X3 receptors in ROS 17/2.8 cells. Example of microscopy photographs of transient P2X3 receptors expression in HEK-293 cells (left) and in rat osteosarcoma cell line ROS17/2.8 (right) after immunofluorescence with anti-P2X3 antibodies. Note that, in contrast to HEK cells, P2X3-immunopositive ROS17/2.8 cells did not show relevant morphological alterations. Scale bar: 50 μm

Article Snippet: For immunoblotting anti-P2X3 (1:300; Alomone, Jerusalem, Israel) and anti-Actin antibodies (1:1000, Sigma) were used.

Techniques: Expressing, Microscopy, Immunofluorescence