Journal: International Journal of Molecular Sciences
Article Title: P2Y 1 and P2Y 12 Receptors Mediate Aggregation of Dog and Cat Platelets: A Comparison to Human Platelets
doi: 10.3390/ijms26031206
Figure Lengend Snippet: Presence of P2Y 1 and P2Y 12 in dog, cat, and human platelets. ( A , B ) PRP was isolated from the whole blood of dogs, cats, or humans to obtain platelets. Whole lysates of platelets were analysed by immunoblotting using an ( A ) anti-P2Y 1 or ( B ) anti-P2Y 12 receptor antibody (top panels) or ( A , B ) anti-actin antibody (bottom panels). Arrows indicate ( A ) P2Y 1 or ( B ) P2Y 12 receptors. Results are representative of three independent experiments.
Article Snippet: Rabbit anti-human anti-P2Y 1 (cat. No. APR-021) and rabbit anti-human anti-P2Y 12 (cat. No. APR-020) polyclonal antibodies were from Alomone Labs (Jerusalem, Israel).
Techniques: Isolation, Western Blot