p2x6  (Alomone Labs)

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: P2X6 , Rabbit , Alomone , APR-013 , 1:200 , 1:4000 , 1:1000.

Techniques:

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques:

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Western blot analysis of P2X receptor expression in the anterior pituitary. a Lane P2X1 to P2X7 are the results detected by P2X1 to P2X7 antibodies, respectively, lane M is the molecular weight marker, note that a strong immunostained band was detected by the P2X4 antibody, followed by the P2X5, P2X3, P2X2, P2X7, and P2X6 receptor antibodies, no band was detected by P2X1 receptor antibody. b Lane P2X1 to P2X7 are the results of the antibody pre-absorption control experiments for P2X1 to P2X7, note that no bands were detected. c The band density ratio of each P2X receptor over GAPDH

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques: Western Blot, Expressing, Molecular Weight, Marker

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Expression of P2X1 to P2X7 receptor-ir (red) in the rat anterior pituitary. a to f P2X1 to P2X7 receptor immunoreactive cells respectively. Note that strong immunostaining was detected using the P2X4 receptor antibody, followed by P2X5, P2X3, P2X6, P2X2, and P2X7 receptor antibodies. No obvious immunostaining was detected by the P2X1 antibody. g A merged image from f and an image of Iba-1-ir cells (green) (a macrophage/microglia marker) in the same field of f. Note that all the P2X7 receptor reactive cells were labeled by Iba-1-ir. All scale bars = 120 μm

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques: Expressing, Immunostaining, Marker, Labeling

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Colocalization of P2X6 receptor-ir (green) with ACTH-ir, LH-ir, TSH-ir (red), and GH and PRL mRNA hybridization signals (red) in rat anterior pituitary. Note that all P2X6 receptor-ir cells were labeled by LH-ir or TSH-ir, but only a minority of LH-ir or TSH-ir cells were also labeled by P2X6-ir. No P2X6 receptor-ir cells were detected with ACT-ir, S100-ir, and GH and PRL mRNA hybridization signals. An arrow indicates a cell double labeled with P2X6-ir and LH-ir or TSH-ir in c and e, respectively. All scale bars = 120 μm

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques: Hybridization, Labeling

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Colocalization of P2X5 receptor-ir (a, red), P2X6 receptor-ir (b, green) and LH-ir (d, blue) in rat anterior pituitary. c The merged image from a and b, note that no P2X5 receptor-ir cells were labeled by P2X6-ir. e The merged image from b and d, note that all the P2X6 receptor-ir cells were labeled with LH-ir, although only a minority of LH-ir cells were labeled with P2X6 receptor-ir. f The merged image from a, b, and d, note that all LH-ir cells were labeled with P2X5 and P2X6 receptor-ir. No LH-ir cells were single labeled. An arrow indicates a cell double labeled with P2X6-ir and LH-ir or P2X5 receptor-ir and LH-ir in e and f, respectively. All scale bars = 120 μm

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques: Labeling

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Colocalization of P2X5 receptor-ir (a, red), P2X6 receptor-ir (b, green), and TSH-ir (d, blue) in rat anterior pituitary. c The merged image from a and b, note that no P2X5 receptor-ir cells were labeled by P2X6-ir. e The merged image from b and d, note that all the P2X6 receptor-ir cells were labeled with TSH-ir, although only a minority of TSH-ir cells were labeled with P2X6 receptor-ir. f The merged image from a, b, and d, note that all TSH-ir cells were labeled with P2X5 and P2X6 receptor-ir. No TSH-ir cells were single labeled. An arrow indicates a cell double labeled with P2X6-ir and TSH-ir or P2X5 receptor-ir and TSH-ir in e and f, respectively. All scale bars = 120 μm

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques: Labeling

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Quantitative analysis of the coexistence between P2X5 and P2X6 receptor-ir cells, and LH-ir and TSH-ir cells (the positive cell number/0.36 mm 2 )

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques:

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Coexistence between P2X receptors and pituitary hormones or S100

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques: