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anti p2x7  (Alomone Labs)


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    Alomone Labs anti p2x7
    Anti P2x7, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apr-004/pm41936614-205-45-47?v=Alomone+Labs
    Average 96 stars, based on 213 article reviews
    anti p2x7 - by Bioz Stars, 2026-07
    96/100 stars

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    Rat P2X7A exhibits macropore activity. ( A ) Representative flow cytometry histograms showing ethidium bromide (EtBr) uptake in HEK293-P2X7A cells stimulated with increasing BzATP concentrations. ( B ) Dose–response curve of EtBr uptake versus log [BzATP], (EC50 = 7.61 ± 1.14 μM, n = 4). ( C ) Representative histograms of EtBr uptake in HEK293 cells expressing <t>P2X7</t> variants: basal (blue), 60 μM BzATP for 20 min (yellow), and Triton X-100 (red, positive control). ( D ) Fold change in FL2 fluorescence relative to baseline at 0, 15, and 20 min. Symbols: ⏺ HEK293, ◯ P2X7A, ▲ P2X7B, ▼ P2X7∆N, ◆ P2X7LOCHF, □ with dash line P2X7DN. Inset Triton X-100 positive control. Bars = mean ± S.E.M. of 6 experiments. * = p < 0.05.
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    Rat P2X7A exhibits macropore activity. ( A ) Representative flow cytometry histograms showing ethidium bromide (EtBr) uptake in HEK293-P2X7A cells stimulated with increasing BzATP concentrations. ( B ) Dose–response curve of EtBr uptake versus log [BzATP], (EC50 = 7.61 ± 1.14 μM, n = 4). ( C ) Representative histograms of EtBr uptake in HEK293 cells expressing <t>P2X7</t> variants: basal (blue), 60 μM BzATP for 20 min (yellow), and Triton X-100 (red, positive control). ( D ) Fold change in FL2 fluorescence relative to baseline at 0, 15, and 20 min. Symbols: ⏺ HEK293, ◯ P2X7A, ▲ P2X7B, ▼ P2X7∆N, ◆ P2X7LOCHF, □ with dash line P2X7DN. Inset Triton X-100 positive control. Bars = mean ± S.E.M. of 6 experiments. * = p < 0.05.
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    Rat P2X7A exhibits macropore activity. ( A ) Representative flow cytometry histograms showing ethidium bromide (EtBr) uptake in HEK293-P2X7A cells stimulated with increasing BzATP concentrations. ( B ) Dose–response curve of EtBr uptake versus log [BzATP], (EC50 = 7.61 ± 1.14 μM, n = 4). ( C ) Representative histograms of EtBr uptake in HEK293 cells expressing <t>P2X7</t> variants: basal (blue), 60 μM BzATP for 20 min (yellow), and Triton X-100 (red, positive control). ( D ) Fold change in FL2 fluorescence relative to baseline at 0, 15, and 20 min. Symbols: ⏺ HEK293, ◯ P2X7A, ▲ P2X7B, ▼ P2X7∆N, ◆ P2X7LOCHF, □ with dash line P2X7DN. Inset Triton X-100 positive control. Bars = mean ± S.E.M. of 6 experiments. * = p < 0.05.
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    Rat P2X7A exhibits macropore activity. ( A ) Representative flow cytometry histograms showing ethidium bromide (EtBr) uptake in HEK293-P2X7A cells stimulated with increasing BzATP concentrations. ( B ) Dose–response curve of EtBr uptake versus log [BzATP], (EC50 = 7.61 ± 1.14 μM, n = 4). ( C ) Representative histograms of EtBr uptake in HEK293 cells expressing P2X7 variants: basal (blue), 60 μM BzATP for 20 min (yellow), and Triton X-100 (red, positive control). ( D ) Fold change in FL2 fluorescence relative to baseline at 0, 15, and 20 min. Symbols: ⏺ HEK293, ◯ P2X7A, ▲ P2X7B, ▼ P2X7∆N, ◆ P2X7LOCHF, □ with dash line P2X7DN. Inset Triton X-100 positive control. Bars = mean ± S.E.M. of 6 experiments. * = p < 0.05.

    Journal: International Journal of Molecular Sciences

    Article Title: Rat P2X7 Receptor Show Functional Independence Between Macropore Formation and Scavenger Activity

    doi: 10.3390/ijms27041922

    Figure Lengend Snippet: Rat P2X7A exhibits macropore activity. ( A ) Representative flow cytometry histograms showing ethidium bromide (EtBr) uptake in HEK293-P2X7A cells stimulated with increasing BzATP concentrations. ( B ) Dose–response curve of EtBr uptake versus log [BzATP], (EC50 = 7.61 ± 1.14 μM, n = 4). ( C ) Representative histograms of EtBr uptake in HEK293 cells expressing P2X7 variants: basal (blue), 60 μM BzATP for 20 min (yellow), and Triton X-100 (red, positive control). ( D ) Fold change in FL2 fluorescence relative to baseline at 0, 15, and 20 min. Symbols: ⏺ HEK293, ◯ P2X7A, ▲ P2X7B, ▼ P2X7∆N, ◆ P2X7LOCHF, □ with dash line P2X7DN. Inset Triton X-100 positive control. Bars = mean ± S.E.M. of 6 experiments. * = p < 0.05.

    Article Snippet: Anti-P2X7 antibodies APR-004 (Alomone Labs, Jerusalem, Israel), diluted 1:500, and PA5-28020 (Thermo Fisher Scientific), diluted 1:1000, were used.

    Techniques: Activity Assay, Flow Cytometry, Expressing, Positive Control, Fluorescence

    Rat P2X7A exhibits calcium influx. ( A ) Representative flow cytometry histograms of Fluo-4 fluorescence in HEK293-P2X7A cells stimulated with increasing BzATP concentrations. ( B ) Representative plots of P2X7 variants; black triangles indicate addition of 60 μM BzATP (1 min) or 3 μg/mL ionomycin positive control (4 min).

    Journal: International Journal of Molecular Sciences

    Article Title: Rat P2X7 Receptor Show Functional Independence Between Macropore Formation and Scavenger Activity

    doi: 10.3390/ijms27041922

    Figure Lengend Snippet: Rat P2X7A exhibits calcium influx. ( A ) Representative flow cytometry histograms of Fluo-4 fluorescence in HEK293-P2X7A cells stimulated with increasing BzATP concentrations. ( B ) Representative plots of P2X7 variants; black triangles indicate addition of 60 μM BzATP (1 min) or 3 μg/mL ionomycin positive control (4 min).

    Article Snippet: Anti-P2X7 antibodies APR-004 (Alomone Labs, Jerusalem, Israel), diluted 1:500, and PA5-28020 (Thermo Fisher Scientific), diluted 1:1000, were used.

    Techniques: Flow Cytometry, Fluorescence, Positive Control

    ( A ) Representative Western blots of phosphorylated and total ERK1/2 in HEK293 WT or rat P2X7 variant–transfected cells stimulated with 60 μM BzATP. ( B ) Quantification of six experiments (pERK/total ERK, arbitrary units). Inset 40 nM PMA positive control. Symbols: ⏺ HEK293, ◯ P2X7A, ▲ P2X7B, ▼ P2X7∆N, ◆ P2X7LOCHF, □ with dash line P2X7DN. Bars = mean ± S.E.M. * = p < 0.05.

    Journal: International Journal of Molecular Sciences

    Article Title: Rat P2X7 Receptor Show Functional Independence Between Macropore Formation and Scavenger Activity

    doi: 10.3390/ijms27041922

    Figure Lengend Snippet: ( A ) Representative Western blots of phosphorylated and total ERK1/2 in HEK293 WT or rat P2X7 variant–transfected cells stimulated with 60 μM BzATP. ( B ) Quantification of six experiments (pERK/total ERK, arbitrary units). Inset 40 nM PMA positive control. Symbols: ⏺ HEK293, ◯ P2X7A, ▲ P2X7B, ▼ P2X7∆N, ◆ P2X7LOCHF, □ with dash line P2X7DN. Bars = mean ± S.E.M. * = p < 0.05.

    Article Snippet: Anti-P2X7 antibodies APR-004 (Alomone Labs, Jerusalem, Israel), diluted 1:500, and PA5-28020 (Thermo Fisher Scientific), diluted 1:1000, were used.

    Techniques: Western Blot, Variant Assay, Transfection, Positive Control

    p38 activation in rat P2X7 variants. ( A ) Representative Western blots of phosphorylated and total p38 in HEK293 WT or P2X7 variant–transfected cells stimulated with 60 μM BzATP. ( B ) Quantification of six experiments (p-p38/total p38, arbitrary units). Symbols: ⏺ HEK293, ◯ P2X7A, ▲ P2X7B, ▼ P2X7∆N, ◆ P2X7LOCHF, □ with dash line P2X7DN. Bars = mean ± S.E.M. * = p < 0.05.

    Journal: International Journal of Molecular Sciences

    Article Title: Rat P2X7 Receptor Show Functional Independence Between Macropore Formation and Scavenger Activity

    doi: 10.3390/ijms27041922

    Figure Lengend Snippet: p38 activation in rat P2X7 variants. ( A ) Representative Western blots of phosphorylated and total p38 in HEK293 WT or P2X7 variant–transfected cells stimulated with 60 μM BzATP. ( B ) Quantification of six experiments (p-p38/total p38, arbitrary units). Symbols: ⏺ HEK293, ◯ P2X7A, ▲ P2X7B, ▼ P2X7∆N, ◆ P2X7LOCHF, □ with dash line P2X7DN. Bars = mean ± S.E.M. * = p < 0.05.

    Article Snippet: Anti-P2X7 antibodies APR-004 (Alomone Labs, Jerusalem, Israel), diluted 1:500, and PA5-28020 (Thermo Fisher Scientific), diluted 1:1000, were used.

    Techniques: Activation Assay, Western Blot, Variant Assay, Transfection

    A740003 inhibits macropore activity of rat P2X7 but not ERK1/2 pathway signaling. ( A ) Quantification of ethidium bromide (EtBr) uptake in HEK P2X7A cells. ( B ) Quantification of ERK1/2 activation in HEK293 WT or HEK293 P2X7A cells. Symbols: ⏺ HEK293, ◯ P2X7A. Bars = mean ± S.E.M. of 4 experiments. * = p < 0.05.

    Journal: International Journal of Molecular Sciences

    Article Title: Rat P2X7 Receptor Show Functional Independence Between Macropore Formation and Scavenger Activity

    doi: 10.3390/ijms27041922

    Figure Lengend Snippet: A740003 inhibits macropore activity of rat P2X7 but not ERK1/2 pathway signaling. ( A ) Quantification of ethidium bromide (EtBr) uptake in HEK P2X7A cells. ( B ) Quantification of ERK1/2 activation in HEK293 WT or HEK293 P2X7A cells. Symbols: ⏺ HEK293, ◯ P2X7A. Bars = mean ± S.E.M. of 4 experiments. * = p < 0.05.

    Article Snippet: Anti-P2X7 antibodies APR-004 (Alomone Labs, Jerusalem, Israel), diluted 1:500, and PA5-28020 (Thermo Fisher Scientific), diluted 1:1000, were used.

    Techniques: Activity Assay, Activation Assay

    Phagocytic activity in rat P2X7 variants. ( A ) Representative flow cytometry histograms showing apoptotic body uptake between 0 and 6 h. ( B ) Quantification of phagocytic cells in HEK293 WT and P2X7A. Symbols: ⏺ HEK293, ◯ P2X7A. ( C ) Histograms of apoptotic body uptake in rat P2X7 variants at 0 h (red) and 6 h (blue). ( D ) Quantification of phagocytic cells per variant. Bars = mean ± S.E.M. of 4–6 experiments. Symbols: ⏺ HEK293, ◯ P2X7A, ▲ P2X7B, ▼ P2X7∆N, ◆ P2X7LOCHF, □ P2X7DN. * = p < 0.05.

    Journal: International Journal of Molecular Sciences

    Article Title: Rat P2X7 Receptor Show Functional Independence Between Macropore Formation and Scavenger Activity

    doi: 10.3390/ijms27041922

    Figure Lengend Snippet: Phagocytic activity in rat P2X7 variants. ( A ) Representative flow cytometry histograms showing apoptotic body uptake between 0 and 6 h. ( B ) Quantification of phagocytic cells in HEK293 WT and P2X7A. Symbols: ⏺ HEK293, ◯ P2X7A. ( C ) Histograms of apoptotic body uptake in rat P2X7 variants at 0 h (red) and 6 h (blue). ( D ) Quantification of phagocytic cells per variant. Bars = mean ± S.E.M. of 4–6 experiments. Symbols: ⏺ HEK293, ◯ P2X7A, ▲ P2X7B, ▼ P2X7∆N, ◆ P2X7LOCHF, □ P2X7DN. * = p < 0.05.

    Article Snippet: Anti-P2X7 antibodies APR-004 (Alomone Labs, Jerusalem, Israel), diluted 1:500, and PA5-28020 (Thermo Fisher Scientific), diluted 1:1000, were used.

    Techniques: Activity Assay, Flow Cytometry, Variant Assay