rabbit polyclonal anti p2x1 antibody  (Alomone Labs)

Journal: Purinergic Signalling

Article Title: Expression of the P2X1 receptor remains in the type II spiral ganglion neurons in the mature rat cochlea

doi: 10.1007/s11302-026-10129-7

Figure Lengend Snippet: P2X1 receptor expression in the adult Wistar rat cochlea. Cryosections ( A - B , E ) and whole mount ( C - D , F ) from adult (6–8 week-old) Wistar rat cochleae were immunolabelled with anti-βIII tubulin ( green ) and anti-P2X1 antibody ( red ), and counterstained with DAPI ( blue ). (A-B) Representative image showing the overviews of rat cochlear cryosections ( A ) and the region with the organ of Corti and Rosenthal’s canal ( B ) showing P2X1 receptor expression in the organ of Corti (OoC) and the spiral ganglion neurons (SGN). (C-D) 6–8-week-old rat cochlea whole mount OoC preparation showing P2X1 immunolabelling, focusing on the SGN processes underneath the inner and outer hair cells ( C ) and the corresponding region in P2X1 antibody peptide block control ( D ). ( E -E”) Rosenthal’s canal of cryosection from ( B ). ( F -F’) 6–8-week-old OoC rat cochlea whole mount z-stack images showing tunnel crossing fibres labelled with anti-P2X1 antibody (arrowheads) and P2X1 immunolabelling at the base of the OoC (*). Scale bars, 50 μ m (A), 40 μ m ( B - E ), 20 μ m ( F ). Representative images from n = 4 cochleae from four different animals

Article Snippet: The primary antibodies used were: rabbit polyclonal anti-P2X1 antibody (Alomone Labs, Jerusalem, Israel, Catalog# APR-001, 1:500 dilution), mouse anti-β III tubulin (IgG2a monoclonal, BioLegend, San Diego, CA, catalog No. 801213, 1:1000 dilution), goat polyclonal anti-peripherin-1 antibody (Everest Biotech, Upper Heyford, UK, catalog No. EB12405, 1:1000 dilution).

Techniques: Expressing, Blocking Assay, Control

Journal: Purinergic Signalling

Article Title: Expression of the P2X1 receptor remains in the type II spiral ganglion neurons in the mature rat cochlea

doi: 10.1007/s11302-026-10129-7

Figure Lengend Snippet: Co-expression of P2X1 receptors and peripherin-1 in Wistar rat cochlea. ( A - C ) Cryosections from adult (6–8 week-old) Wistar rat cochleae were immunolabelled with anti-βIII tubulin (green) and anti-P2X1 antibody (red), and counterstained with DAPI (blue). ( F ) White arrows indicate SGNs with strong P2X1 signals relative to other SGNs. Images were taken of the basal ( A ), mid-( B ) and apical ( C ) regions of the cochlea. Scale bar = 20 μm for ( A - C ). ( D - G ) Cryosections from 6–8-week-old Wistar rat cochlea ( D - E ) and P8 cochlea ( F - G ) were co-labelled with anti-peripherin-1 antibody ( green ), and anti-P2X1 antibody ( red ). Images show the overview from the apical turn ( F ) and spiral ganglion neurons in the apical region ( G -G”). Representative images from n = 4 cochleae from four different animals

Article Snippet: The primary antibodies used were: rabbit polyclonal anti-P2X1 antibody (Alomone Labs, Jerusalem, Israel, Catalog# APR-001, 1:500 dilution), mouse anti-β III tubulin (IgG2a monoclonal, BioLegend, San Diego, CA, catalog No. 801213, 1:1000 dilution), goat polyclonal anti-peripherin-1 antibody (Everest Biotech, Upper Heyford, UK, catalog No. EB12405, 1:1000 dilution).

Techniques: Expressing

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Molecular mechanisms of voiding dysfunction in a novel mouse model of acute urinary retention

doi: 10.1096/fj.202002415R

Figure Lengend Snippet: Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001

Article Snippet: Western blot analysis Rabbit anti-P2X1 antibody (1:1000, #APR-001) and rabbit anti-chrm2 antibody (1:1000, #AMR-002) were purchased from Alomone Lab. Rabbit anti-chrm3 antibody (1:1000, #PA5–77485) was purchased from Thermo Fisher Scientific.

Techniques: Expressing, Western Blot, Control