applied biosystems steponplus instrument  (Thermo Fisher)


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    Thermo Fisher applied biosystems steponplus instrument
    Applied Biosystems Steponplus Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/applied biosystems steponplus instrument/product/Thermo Fisher
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    applied biosystems steponplus instrument - by Bioz Stars, 2020-04
    87/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Robust Utilization of Phospholipase-Generated Metabolites, Glycerophosphodiesters, by Candida albicans: Role of the CaGit1 Permease ▿
    Article Snippet: Paragraph title: RNA Extraction and quantitative reverse transcriptase PCR (qRT-PCR) gene expression analysis. ... Experimental samples were analyzed in triplicate on an Applied Biosystems StepOnPlus instrument.

    Amplification:

    Article Title: Robust Utilization of Phospholipase-Generated Metabolites, Glycerophosphodiesters, by Candida albicans: Role of the CaGit1 Permease ▿
    Article Snippet: Experimental samples were analyzed in triplicate on an Applied Biosystems StepOnPlus instrument. .. Reverse transcription was carried out at 48°C for 30 min, followed by 95°C for 10 min for RT inactivation and polymerase activation, followed by 40 cycles of 95°C for 15 s, 50°C for 30 s, and 72°C for 40 s for amplification, followed by the melt curve to check for primer specificity.

    Polymerase Chain Reaction:

    Article Title: Robust Utilization of Phospholipase-Generated Metabolites, Glycerophosphodiesters, by Candida albicans: Role of the CaGit1 Permease ▿
    Article Snippet: Paragraph title: RNA Extraction and quantitative reverse transcriptase PCR (qRT-PCR) gene expression analysis. ... Experimental samples were analyzed in triplicate on an Applied Biosystems StepOnPlus instrument.

    Article Title: Preferential production of G-CSF by a protein-like Lactobacillus rhamnosus GR-1 secretory factor through activating TLR2-dependent signaling events without activation of JNKs
    Article Snippet: .. The Q-PCR was performed using the RT2 Profiler PCR Array System Kit for mouse cytokines and chemokines (QIAGEN) on an Applied Biosystems StepOnPlus instrument according to the manufacturer guidelines. ..

    Isolation:

    Article Title: Preferential production of G-CSF by a protein-like Lactobacillus rhamnosus GR-1 secretory factor through activating TLR2-dependent signaling events without activation of JNKs
    Article Snippet: Cytokines and Chemokines PCR Array Total RNA was isolated using RNeasy Mini kit (QIAGEN Canada) according to the manufacture’s recommendations. .. The Q-PCR was performed using the RT2 Profiler PCR Array System Kit for mouse cytokines and chemokines (QIAGEN) on an Applied Biosystems StepOnPlus instrument according to the manufacturer guidelines.

    Quantitative RT-PCR:

    Article Title: Robust Utilization of Phospholipase-Generated Metabolites, Glycerophosphodiesters, by Candida albicans: Role of the CaGit1 Permease ▿
    Article Snippet: Paragraph title: RNA Extraction and quantitative reverse transcriptase PCR (qRT-PCR) gene expression analysis. ... Experimental samples were analyzed in triplicate on an Applied Biosystems StepOnPlus instrument.

    SYBR Green Assay:

    Article Title: Robust Utilization of Phospholipase-Generated Metabolites, Glycerophosphodiesters, by Candida albicans: Role of the CaGit1 Permease ▿
    Article Snippet: Real-time RT-PCR was performed using the Power SYBR green RNA-to-CT 1-Step kit (Applied Biosystems). .. Experimental samples were analyzed in triplicate on an Applied Biosystems StepOnPlus instrument.

    Activation Assay:

    Article Title: Robust Utilization of Phospholipase-Generated Metabolites, Glycerophosphodiesters, by Candida albicans: Role of the CaGit1 Permease ▿
    Article Snippet: Experimental samples were analyzed in triplicate on an Applied Biosystems StepOnPlus instrument. .. Reverse transcription was carried out at 48°C for 30 min, followed by 95°C for 10 min for RT inactivation and polymerase activation, followed by 40 cycles of 95°C for 15 s, 50°C for 30 s, and 72°C for 40 s for amplification, followed by the melt curve to check for primer specificity.

    Incubation:

    Article Title: Robust Utilization of Phospholipase-Generated Metabolites, Glycerophosphodiesters, by Candida albicans: Role of the CaGit1 Permease ▿
    Article Snippet: A 5-μg sample of RNA was treated with 2 units of DNase and incubated at 37°C for 30 min. .. Experimental samples were analyzed in triplicate on an Applied Biosystems StepOnPlus instrument.

    Expressing:

    Article Title: Robust Utilization of Phospholipase-Generated Metabolites, Glycerophosphodiesters, by Candida albicans: Role of the CaGit1 Permease ▿
    Article Snippet: Paragraph title: RNA Extraction and quantitative reverse transcriptase PCR (qRT-PCR) gene expression analysis. ... Experimental samples were analyzed in triplicate on an Applied Biosystems StepOnPlus instrument.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Robust Utilization of Phospholipase-Generated Metabolites, Glycerophosphodiesters, by Candida albicans: Role of the CaGit1 Permease ▿
    Article Snippet: Each reaction mixture consisted of 0.2 μl of a 125× RT enzyme mix, 12.5 μl of a 2× RT-PCR mix, 500 nM primers, and 1.5 μl DNase-treated RNA in a total volume of 25 μl. .. Experimental samples were analyzed in triplicate on an Applied Biosystems StepOnPlus instrument.

    Software:

    Article Title: Robust Utilization of Phospholipase-Generated Metabolites, Glycerophosphodiesters, by Candida albicans: Role of the CaGit1 Permease ▿
    Article Snippet: Primer 3 software ( ) was used to design primers for CaGIT1 (forward, 5′-CGCATCTTTGTCAACTCAAG-3′; reverse, 5′-TAGCAGCTTCACTTGCTGTC-3′). .. Experimental samples were analyzed in triplicate on an Applied Biosystems StepOnPlus instrument.

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  • 99
    Thermo Fisher applied biosystems steponeplus instrument
    Applied Biosystems Steponeplus Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/applied biosystems steponeplus instrument/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    applied biosystems steponeplus instrument - by Bioz Stars, 2020-04
    99/100 stars
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