applied biosystems geneamp 9700  (Thermo Fisher)


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    Name:
    Shandon Grosslab Senior Pathology Workstations
    Description:
    Multiple options to suit your specific needs Thermo Scientific Shandon Grosslab Senior Pathology Workstations provide superior quality and a variety of features
    Catalog Number:
    970022
    Price:
    None
    Applications:
    Anatomical Pathology|Clinical
    Category:
    Instruments and Equipment
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    Thermo Fisher applied biosystems geneamp 9700
    Multiple options to suit your specific needs Thermo Scientific Shandon Grosslab Senior Pathology Workstations provide superior quality and a variety of features
    https://www.bioz.com/result/applied biosystems geneamp 9700/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    applied biosystems geneamp 9700 - by Bioz Stars, 2020-09
    99/100 stars

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    Polymerase Chain Reaction:

    Article Title: Development of a fast PCR protocol enabling rapid generation of AmpFlSTR® Identifiler® profiles for genotyping of human DNA
    Article Snippet: .. The estimated duration time for this protocol is 3.5 hours when using a GeneAmp® PCR System 9700 thermal cycler (Applied Biosystems, Foster City, CA, USA) at the 9600 emulation mode (recommended by the manufacturer). .. The strategy employed in order to reduce time allotted to PCR amplification included the replacement of AmpliTaq Gold® with SpeedSTAR™ DNA polymerase, the use of a thermal cycler with greater efficiency (Bio-Rad C1000™, maximum ramp rate of 5°C/s) [ ] and modification of the cycling parameters including the adoption of a two-step PCR approach (where the annealing and extension steps are combined) and decreased hold times at all steps.

    Article Title: Highly sensitive amperometric detection of genomic DNA in animal tissues
    Article Snippet: .. First strand cDNA synthesis was carried out by incubating the mixture on a DNA thermal cycler (Gene Amp PCR System 9700, Applied Biosystems, Foster City) at 56°C for 50 min. .. The cDNA was subsequently used as template for the PCR amplification and biotinylation.

    Article Title: Molecular characterisation of Mycoplasma species isolated from the genital tract of Dorper sheep in South Africa
    Article Snippet: .. The mixtures were subjected to 10 minutes of initial denaturation at 94 ºC, followed by 35 cycles of amplification involving denaturation at 94 ºC for 30 seconds, primer annealing at 59 ºC for 45 seconds, primer extension at 72 ºC for 45 seconds, and a final primer extension at 72 ºC for 7 minutes, using a DNA thermal cycler (GeneAmp PCR system 9700, Applied Biosystems, SA). .. Amplified products were analysed together with a DNA ladder (O'Gene rulerTM , Fermentas Life Sciences, Inqaba Biotechnical, Industries [Pty] Ltd, Pretoria, SA) on a 1.5% agarose gel (Celtic Molecular Diagnostics, SA).

    Article Title: Detection of macrolide and disinfectant resistance genes in clinical Staphylococcus aureus and coagulase-negative staphylococci
    Article Snippet: .. A final elongation at 72°C for 10 min was achieved in a DNA thermal cycler (GenAmp PCR system 9700-Applied Biosystem Int., USA). ..

    Article Title: Lacrimal Proline Rich 4 (LPRR4) Protein in the Tear Fluid Is a Potential Biomarker of Dry Eye Syndrome
    Article Snippet: .. RNA was extracted from the tissues using TRI reagent method, cDNA conversion was done from RNA using iScript™ cDNA synthesis kit (Bio-Rad, Herclus,CA) and Reverse transcriptase PCR (GeneAmp PCR system 9700 from Applied Biosystems) was done for LPRR4. ..

    Article Title: Novel Fluorescent Ligase Detection Reaction and Flow Cytometric Analysis of SYT-SSX Fusions in Synovial Sarcoma
    Article Snippet: .. The ligase detection reactions (LDR) were carried out in a PCR machine (Gene-Amp 9700; Applied Biosystems Inc.) as follows: 95°C for 5 minutes, 20 cycles of 95°C for 30 seconds, 65°C for 2 minutes, then 95°C for 5 minutes and cooling to 4°C. .. Following LDR, a 10-μl aliquot of ligation product was mixed with 10 μl of 6.0–8.0 μm streptavidin-coated polystyrene microbeads (Spherotech, Inc., Libertyville, IL) in a microfuge tube and incubated at room temperature for 30 minutes, with brief agitation of the tube at 15 minutes.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Effects of Different Ligands in the Notch Signaling Pathway on the Proliferation and Transdifferentiation of Primary Type II Alveolar Epithelial Cells
    Article Snippet: .. Detection of mRNA Expression of SP-C, AQP5, Dlk1, Jagged1, Notch1, and Hes1SP-C, AQP5, Dlk1, Jagged1, Notch1, and Hes1 mRNAs were detected by reverse transcription (RT)-PCR (ABI 9700, Applied Biosystems, Foster City, CA, USA). ..

    Amplification:

    Article Title: Molecular characterisation of Mycoplasma species isolated from the genital tract of Dorper sheep in South Africa
    Article Snippet: .. The mixtures were subjected to 10 minutes of initial denaturation at 94 ºC, followed by 35 cycles of amplification involving denaturation at 94 ºC for 30 seconds, primer annealing at 59 ºC for 45 seconds, primer extension at 72 ºC for 45 seconds, and a final primer extension at 72 ºC for 7 minutes, using a DNA thermal cycler (GeneAmp PCR system 9700, Applied Biosystems, SA). .. Amplified products were analysed together with a DNA ladder (O'Gene rulerTM , Fermentas Life Sciences, Inqaba Biotechnical, Industries [Pty] Ltd, Pretoria, SA) on a 1.5% agarose gel (Celtic Molecular Diagnostics, SA).

    Expressing:

    Article Title: Effects of Different Ligands in the Notch Signaling Pathway on the Proliferation and Transdifferentiation of Primary Type II Alveolar Epithelial Cells
    Article Snippet: .. Detection of mRNA Expression of SP-C, AQP5, Dlk1, Jagged1, Notch1, and Hes1SP-C, AQP5, Dlk1, Jagged1, Notch1, and Hes1 mRNAs were detected by reverse transcription (RT)-PCR (ABI 9700, Applied Biosystems, Foster City, CA, USA). ..

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  • 99
    Thermo Fisher geneamp pcr system 9700
    Geneamp Pcr System 9700, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2591 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/geneamp pcr system 9700/product/Thermo Fisher
    Average 99 stars, based on 2591 article reviews
    Price from $9.99 to $1999.99
    geneamp pcr system 9700 - by Bioz Stars, 2020-09
    99/100 stars
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