applied biosystem steponeplus  (Thermo Fisher)


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    Structured Review

    Thermo Fisher applied biosystem steponeplus
    Applied Biosystem Steponeplus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/applied biosystem steponeplus/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    applied biosystem steponeplus - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Chronic low dose arsenic exposure preferentially perturbs mitotic phase of the cell cycle
    Article Snippet: .. Quantitative real time-PCR Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Article Title: High-throughput analysis of the RNA-induced silencing complex in myotonic dystrophy type 1 patients identifies the dysregulation of miR-29c and its target ASB2
    Article Snippet: .. For both miRNAs and mRNAs, qPCR was performed using an Applied Biosystem StepOnePlus or 7500 Fast Real-Time PCR System; relative expression was calculated using the comparative Ct method (2−ΔΔCt). .. qPCR analysis of small repeated RNAs Total RNAs and immunoprecipitated RNAs were poly-adenylated in vitro using the Poly(A) Tailing Kit (Applied Biosystems, ThermoFisher Scientific, Waltham, MA) following the manufacturer’s instructions.

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: Paragraph title: 4.6. Total RNA Isolation and Quantitative Real-Time PCR (qRT-PCR) ... The relative abundance of Nrf2 and its downstream antioxidant genes; NQO1, PRDX1, SOD1 and CAT was quantified using iTaq™ Universal SYBR® Green Supermix (Bio-Rad Laboratories GmbH, München, Germany) in Applied Biosystem® StepOnePlus™ (Applied Biosystems, CA, USA) using gene specific primers ( ).

    Article Title: A lethal synergy induced by phellinus linteus and camptothecin11 in colon cancer cells
    Article Snippet: .. Quantitative real time-PCR Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Article Title: Endogenous ribosomal protein L29 (RPL29): a newly identified regulator of angiogenesis in mice
    Article Snippet: .. Real-time quantitative PCR (qPCR) reactions were performed in triplicate using the Applied Biosystem StepOnePlus. .. Each reaction was performed in an individual well of a 96-well plate and made up to 20 μl containing 2–5 ng cDNA, 10 μl SYBR green (Applied Biosystems), 50–300 nM forward and reverse primer and H2 O.

    Article Title: A lethal synergy induced by phellinus linteus and camptothecin11 in colon cancer cells
    Article Snippet: .. Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Transfection:

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: Total RNA Isolation and Quantitative Real-Time PCR (qRT-PCR) Cells were harvested 24-h post-treatment (miRNA, siRNA transfection and que supplementation), subjected to total RNA isolation using miRNeasy® mini kit (Qiagen GmbH, Hilden, Germany) following manufacturer’s protocol. .. The relative abundance of Nrf2 and its downstream antioxidant genes; NQO1, PRDX1, SOD1 and CAT was quantified using iTaq™ Universal SYBR® Green Supermix (Bio-Rad Laboratories GmbH, München, Germany) in Applied Biosystem® StepOnePlus™ (Applied Biosystems, CA, USA) using gene specific primers ( ).

    Amplification:

    Article Title: Chronic low dose arsenic exposure preferentially perturbs mitotic phase of the cell cycle
    Article Snippet: .. Quantitative real time-PCR Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Article Title: A Low-dose Arsenic-induced p53 Protein-mediated Metabolic Mechanism of Radiotherapy Protection *
    Article Snippet: .. 1 μg of total RNA was used to make cDNA (iScript cDNA synthesis kit, Bio-Rad), following the instructions of the manufacturer, which was subsequently used for the amplification by quantitative RT-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Article Title: A lethal synergy induced by phellinus linteus and camptothecin11 in colon cancer cells
    Article Snippet: .. Quantitative real time-PCR Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Article Title: UXT, a novel MDMX-binding protein, promotes glycolysis by mitigating p53-mediated restriction of NF-κB activity
    Article Snippet: .. 1 μg of total RNA was used to make cDNA (iScript cDNA synthesis kit, Bio-Rad), following the manufacturer's instruction, which was subsequently used for the amplification by quantitative RT-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich, St Louis, MO). ..

    Article Title: A lethal synergy induced by phellinus linteus and camptothecin11 in colon cancer cells
    Article Snippet: .. Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Isolation:

    Article Title: Chronic low dose arsenic exposure preferentially perturbs mitotic phase of the cell cycle
    Article Snippet: .. Quantitative real time-PCR Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Article Title: A Low-dose Arsenic-induced p53 Protein-mediated Metabolic Mechanism of Radiotherapy Protection *
    Article Snippet: Paragraph title: RNA Isolation and Quantitative RT-PCR ... 1 μg of total RNA was used to make cDNA (iScript cDNA synthesis kit, Bio-Rad), following the instructions of the manufacturer, which was subsequently used for the amplification by quantitative RT-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich).

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: Paragraph title: 4.6. Total RNA Isolation and Quantitative Real-Time PCR (qRT-PCR) ... The relative abundance of Nrf2 and its downstream antioxidant genes; NQO1, PRDX1, SOD1 and CAT was quantified using iTaq™ Universal SYBR® Green Supermix (Bio-Rad Laboratories GmbH, München, Germany) in Applied Biosystem® StepOnePlus™ (Applied Biosystems, CA, USA) using gene specific primers ( ).

    Article Title: A lethal synergy induced by phellinus linteus and camptothecin11 in colon cancer cells
    Article Snippet: .. Quantitative real time-PCR Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Article Title: UXT, a novel MDMX-binding protein, promotes glycolysis by mitigating p53-mediated restriction of NF-κB activity
    Article Snippet: Paragraph title: RNA isolation and quantitative RT-PCR ... 1 μg of total RNA was used to make cDNA (iScript cDNA synthesis kit, Bio-Rad), following the manufacturer's instruction, which was subsequently used for the amplification by quantitative RT-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich, St Louis, MO).

    Article Title: A lethal synergy induced by phellinus linteus and camptothecin11 in colon cancer cells
    Article Snippet: .. Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Cell Culture:

    Article Title: High-throughput analysis of the RNA-induced silencing complex in myotonic dystrophy type 1 patients identifies the dysregulation of miR-29c and its target ASB2
    Article Snippet: For mRNA analysis, retro-transcription was performed with the SuperScript IV Reverse Transcriptase (ThermoFisher Scientific, Waltham, MA) using oligo (dT) and random primers for cultured myoblasts and with Ovation qPCR system (Nugen, San Carlo, CA) for human biopsies. .. For both miRNAs and mRNAs, qPCR was performed using an Applied Biosystem StepOnePlus or 7500 Fast Real-Time PCR System; relative expression was calculated using the comparative Ct method (2−ΔΔCt).

    SYBR Green Assay:

    Article Title: Chronic low dose arsenic exposure preferentially perturbs mitotic phase of the cell cycle
    Article Snippet: .. Quantitative real time-PCR Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Article Title: High-throughput analysis of the RNA-induced silencing complex in myotonic dystrophy type 1 patients identifies the dysregulation of miR-29c and its target ASB2
    Article Snippet: Power SYBR Green PCR master mix (Applied Biosystems, ThermoFisher Scientific, Waltham, MA) was used to analyze mRNAs. .. For both miRNAs and mRNAs, qPCR was performed using an Applied Biosystem StepOnePlus or 7500 Fast Real-Time PCR System; relative expression was calculated using the comparative Ct method (2−ΔΔCt).

    Article Title: A Low-dose Arsenic-induced p53 Protein-mediated Metabolic Mechanism of Radiotherapy Protection *
    Article Snippet: .. 1 μg of total RNA was used to make cDNA (iScript cDNA synthesis kit, Bio-Rad), following the instructions of the manufacturer, which was subsequently used for the amplification by quantitative RT-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: .. The relative abundance of Nrf2 and its downstream antioxidant genes; NQO1, PRDX1, SOD1 and CAT was quantified using iTaq™ Universal SYBR® Green Supermix (Bio-Rad Laboratories GmbH, München, Germany) in Applied Biosystem® StepOnePlus™ (Applied Biosystems, CA, USA) using gene specific primers ( ). ..

    Article Title: A lethal synergy induced by phellinus linteus and camptothecin11 in colon cancer cells
    Article Snippet: .. Quantitative real time-PCR Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Article Title: Endogenous ribosomal protein L29 (RPL29): a newly identified regulator of angiogenesis in mice
    Article Snippet: Real-time quantitative PCR (qPCR) reactions were performed in triplicate using the Applied Biosystem StepOnePlus. .. Each reaction was performed in an individual well of a 96-well plate and made up to 20 μl containing 2–5 ng cDNA, 10 μl SYBR green (Applied Biosystems), 50–300 nM forward and reverse primer and H2 O.

    Article Title: A lethal synergy induced by phellinus linteus and camptothecin11 in colon cancer cells
    Article Snippet: .. Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Concentration Assay:

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: Briefly, RNA concentration was adjusted using nuclease-free water to total volume 10 µL from each replicate was followed by co-incubation with 0.5 µL oligo (dT)18 and 0.5 µL random primer at 65 °C for 5 min. Next, 1 µL RiboLock, 4 µL 5× reaction buffer, 2 µL dNTPs and 2 µL reverse transcriptase were added for each sample and co-incubated at 25 °C for 5 min, 37 °C for 60 min, and 70 °C for 5 min. After incubation, samples were stored at −20 °C till gene expression analysis. .. The relative abundance of Nrf2 and its downstream antioxidant genes; NQO1, PRDX1, SOD1 and CAT was quantified using iTaq™ Universal SYBR® Green Supermix (Bio-Rad Laboratories GmbH, München, Germany) in Applied Biosystem® StepOnePlus™ (Applied Biosystems, CA, USA) using gene specific primers ( ).

    Incubation:

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: Briefly, RNA concentration was adjusted using nuclease-free water to total volume 10 µL from each replicate was followed by co-incubation with 0.5 µL oligo (dT)18 and 0.5 µL random primer at 65 °C for 5 min. Next, 1 µL RiboLock, 4 µL 5× reaction buffer, 2 µL dNTPs and 2 µL reverse transcriptase were added for each sample and co-incubated at 25 °C for 5 min, 37 °C for 60 min, and 70 °C for 5 min. After incubation, samples were stored at −20 °C till gene expression analysis. .. The relative abundance of Nrf2 and its downstream antioxidant genes; NQO1, PRDX1, SOD1 and CAT was quantified using iTaq™ Universal SYBR® Green Supermix (Bio-Rad Laboratories GmbH, München, Germany) in Applied Biosystem® StepOnePlus™ (Applied Biosystems, CA, USA) using gene specific primers ( ).

    Quantitative RT-PCR:

    Article Title: A Low-dose Arsenic-induced p53 Protein-mediated Metabolic Mechanism of Radiotherapy Protection *
    Article Snippet: .. 1 μg of total RNA was used to make cDNA (iScript cDNA synthesis kit, Bio-Rad), following the instructions of the manufacturer, which was subsequently used for the amplification by quantitative RT-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). ..

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: Paragraph title: 4.6. Total RNA Isolation and Quantitative Real-Time PCR (qRT-PCR) ... The relative abundance of Nrf2 and its downstream antioxidant genes; NQO1, PRDX1, SOD1 and CAT was quantified using iTaq™ Universal SYBR® Green Supermix (Bio-Rad Laboratories GmbH, München, Germany) in Applied Biosystem® StepOnePlus™ (Applied Biosystems, CA, USA) using gene specific primers ( ).

    Article Title: UXT, a novel MDMX-binding protein, promotes glycolysis by mitigating p53-mediated restriction of NF-κB activity
    Article Snippet: .. 1 μg of total RNA was used to make cDNA (iScript cDNA synthesis kit, Bio-Rad), following the manufacturer's instruction, which was subsequently used for the amplification by quantitative RT-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich, St Louis, MO). ..

    Expressing:

    Article Title: Chronic low dose arsenic exposure preferentially perturbs mitotic phase of the cell cycle
    Article Snippet: Quantitative real time-PCR Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). .. The n-fold change in mRNA expression was then determined.

    Article Title: High-throughput analysis of the RNA-induced silencing complex in myotonic dystrophy type 1 patients identifies the dysregulation of miR-29c and its target ASB2
    Article Snippet: .. For both miRNAs and mRNAs, qPCR was performed using an Applied Biosystem StepOnePlus or 7500 Fast Real-Time PCR System; relative expression was calculated using the comparative Ct method (2−ΔΔCt). .. qPCR analysis of small repeated RNAs Total RNAs and immunoprecipitated RNAs were poly-adenylated in vitro using the Poly(A) Tailing Kit (Applied Biosystems, ThermoFisher Scientific, Waltham, MA) following the manufacturer’s instructions.

    Article Title: A Low-dose Arsenic-induced p53 Protein-mediated Metabolic Mechanism of Radiotherapy Protection *
    Article Snippet: 1 μg of total RNA was used to make cDNA (iScript cDNA synthesis kit, Bio-Rad), following the instructions of the manufacturer, which was subsequently used for the amplification by quantitative RT-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). .. The n-fold change in mRNA expression was determined on the basis of the ΔΔCt value.

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: Briefly, RNA concentration was adjusted using nuclease-free water to total volume 10 µL from each replicate was followed by co-incubation with 0.5 µL oligo (dT)18 and 0.5 µL random primer at 65 °C for 5 min. Next, 1 µL RiboLock, 4 µL 5× reaction buffer, 2 µL dNTPs and 2 µL reverse transcriptase were added for each sample and co-incubated at 25 °C for 5 min, 37 °C for 60 min, and 70 °C for 5 min. After incubation, samples were stored at −20 °C till gene expression analysis. .. The relative abundance of Nrf2 and its downstream antioxidant genes; NQO1, PRDX1, SOD1 and CAT was quantified using iTaq™ Universal SYBR® Green Supermix (Bio-Rad Laboratories GmbH, München, Germany) in Applied Biosystem® StepOnePlus™ (Applied Biosystems, CA, USA) using gene specific primers ( ).

    Article Title: A lethal synergy induced by phellinus linteus and camptothecin11 in colon cancer cells
    Article Snippet: Quantitative real time-PCR Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). .. The n-fold change in mRNA expression was determined on the basis of the ΔΔCt value.

    Article Title: UXT, a novel MDMX-binding protein, promotes glycolysis by mitigating p53-mediated restriction of NF-κB activity
    Article Snippet: 1 μg of total RNA was used to make cDNA (iScript cDNA synthesis kit, Bio-Rad), following the manufacturer's instruction, which was subsequently used for the amplification by quantitative RT-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich, St Louis, MO). .. The n-fold change in mRNAs expression was determined on the basis of ΔΔC t value.

    Article Title: A lethal synergy induced by phellinus linteus and camptothecin11 in colon cancer cells
    Article Snippet: Total RNA was isolated using TRIzol reagent (Invitrogen) according to the instruction provided by the manufacturers. cDNA was prepared using 1 μg of total RNA extracted (iScript cDNA synthesis kit, Bio-Rad), and then subjected for the amplification by quantitative real time-PCR using Applied Biosystem StepOnePlus in the presence of SYBR Green JumpStart (Sigma-Aldrich). .. The n-fold change in mRNA expression was determined on the basis of the ΔΔCt value.

    Polymerase Chain Reaction:

    Article Title: High-throughput analysis of the RNA-induced silencing complex in myotonic dystrophy type 1 patients identifies the dysregulation of miR-29c and its target ASB2
    Article Snippet: Power SYBR Green PCR master mix (Applied Biosystems, ThermoFisher Scientific, Waltham, MA) was used to analyze mRNAs. .. For both miRNAs and mRNAs, qPCR was performed using an Applied Biosystem StepOnePlus or 7500 Fast Real-Time PCR System; relative expression was calculated using the comparative Ct method (2−ΔΔCt).

    Article Title: Endogenous ribosomal protein L29 (RPL29): a newly identified regulator of angiogenesis in mice
    Article Snippet: Real-time quantitative PCR (qPCR) reactions were performed in triplicate using the Applied Biosystem StepOnePlus. .. Conditions for the PCR reaction were: 2 minutes at 50°C, 10 minutes at 95°C and then 40 cycles, each consisting of 15 seconds at 95°C and 1 minute at 60°C.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: .. The relative abundance of Nrf2 and its downstream antioxidant genes; NQO1, PRDX1, SOD1 and CAT was quantified using iTaq™ Universal SYBR® Green Supermix (Bio-Rad Laboratories GmbH, München, Germany) in Applied Biosystem® StepOnePlus™ (Applied Biosystems, CA, USA) using gene specific primers ( ). ..

    Spectrophotometry:

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: After assessing the quality and concentration of the RNA samples using NanoDrop 8000 spectrophotometer (NanoDrop technologies, Schwerte, Germany), cDNA synthesis was performed using first stand cDNA synthesis kit (Thermo Fisher scientific, Schwerte, Germany). .. The relative abundance of Nrf2 and its downstream antioxidant genes; NQO1, PRDX1, SOD1 and CAT was quantified using iTaq™ Universal SYBR® Green Supermix (Bio-Rad Laboratories GmbH, München, Germany) in Applied Biosystem® StepOnePlus™ (Applied Biosystems, CA, USA) using gene specific primers ( ).

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher applied biosystems steponeplus
    Applied Biosystems Steponeplus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/applied biosystems steponeplus/product/Thermo Fisher
    Average 90 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    applied biosystems steponeplus - by Bioz Stars, 2020-03
    90/100 stars
      Buy from Supplier

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