Journal: Regenerative Therapy

Article Title: Wharton's jelly mesenchymal stem cell-secretome enhances skin rejuvenation via ApoA4 and SERPINH1

doi: 10.1016/j.reth.2026.101071

Figure Lengend Snippet: Secretome profiling and key bioactive components of the WJ-MSC secretome. (A) Antibody-array heatmap of secreted proteins from AD-, BM-, and WJ-MSC secretomes; values are log 2 -transformed, normalized intensities expressed as fold-change vs Vehicle-CM (color scale: blue = lower, red = higher). (B) STRING (v11.5) protein-protein interaction networks of proteins enriched in the WJ-MSC secretome; nodes are colored by log 2 fold-change (WJ vs AD/BM) and grouped into GO Biological Process clusters relevant to skin rejuvenation (angiogenesis, immune regulation, wound healing, collagen/ECM organization & elasticity, skin-barrier function, oxidative-stress response). Only high-confidence edges (score >0.7) are shown. (C) GO-based functional classification of significantly upregulated proteins (log 2 fold-change >5), highlighting ApoA4 and SERPINH1 as candidates taken forward for functional validation.

Article Snippet: Small interfering RNAs (siRNAs) targeting SERPINH1 and ApoA4 (Bioneer, Daejeon, Korea) were transfected into cells at a final concentration of 30 nM using standard transfection reagent protocols.

Techniques: Ab Array, Transformation Assay, Functional Assay, Biomarker Discovery

Journal: Regenerative Therapy

Article Title: Wharton's jelly mesenchymal stem cell-secretome enhances skin rejuvenation via ApoA4 and SERPINH1

doi: 10.1016/j.reth.2026.101071

Figure Lengend Snippet: Gene and protein expression of ApoA4 and SERPINH1 across MSC sources. (A) Relative mRNA expression of APOA4 and SERPINH1 in AD-MSCs, BM-MSCs, and WJ-MSCs by qPCR ( GAPDH -normalized; calibration as in Methods). (B) Secreted protein levels of ApoA4 and SERPINH1 in the corresponding secretomes by ELISA. Under the conditions tested, WJ (WJ-MSC secretome) showed higher APOA4/SERPINH1 mRNA and higher ApoA4/SERPINH1 protein than AD (AD-MSC secretome) or BM (BM-MSC secretome). Data are mean ± SEM; one-way ANOVA with Tukey's post hoc test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001).

Article Snippet: Small interfering RNAs (siRNAs) targeting SERPINH1 and ApoA4 (Bioneer, Daejeon, Korea) were transfected into cells at a final concentration of 30 nM using standard transfection reagent protocols.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Regenerative Therapy

Article Title: Wharton's jelly mesenchymal stem cell-secretome enhances skin rejuvenation via ApoA4 and SERPINH1

doi: 10.1016/j.reth.2026.101071

Figure Lengend Snippet: ApoA4 in vitro functions relevant to skin rejuvenation. Baseline control: SFM (no serum/hPL); Vehicle-CM not used. (A) HaCaT scratch-wound assay showing concentration-dependent increases in migration/closure with ApoA4. (B) In LPS-stimulated RAW 264.7 macrophages, ApoA4 reduces mRNA levels of IL-6, COX-2, IL-1β, and TNF-α. (C) DCF-DA staining shows decreased intracellular ROS with ApoA4; representative images confirm fewer ROS-positive cells, comparable to ascorbic acid (10 μM). (D) Immunoblot verifying siApoA4 knockdown. (E) Total antioxidant capacity increased with ApoA4; siApoA4 reduced capacity toward control levels. Total antioxidant capacity was calculated from a Trolox standard curve and is presented as Trolox equivalents (nmol/μL). Data are mean ± SEM; one-way ANOVA with Tukey's post hoc test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001).

Article Snippet: Small interfering RNAs (siRNAs) targeting SERPINH1 and ApoA4 (Bioneer, Daejeon, Korea) were transfected into cells at a final concentration of 30 nM using standard transfection reagent protocols.

Techniques: In Vitro, Control, Scratch Wound Assay Assay, Concentration Assay, Migration, Staining, Western Blot, Knockdown

Journal: Journal of Human Immunity

Article Title: A novel heterozygous pathogenic AIRE variant causing autoimmunity but not infectious susceptibility

doi: 10.70962/jhi.20250151

Figure Lengend Snippet: DN impact of AIRE C337F on WT AIRE-mediated gene transcription. HEK-293T cell lines were transfected with plasmids encoding either WT AIRE or AIRE R257X , AIRE C311Y or AIRE C337F variants alone, or equal amounts WT AIRE together with AIRE R257X , AIRE C311Y , or AIRE C337F . (A and B) Transcriptional activity was assessed by measuring expression levels of (A) known AIRE -regulated genes KRT14 , IGFL1 , S100A8 , APOA4 , and INS or (B) non- AIRE -regulated genes CCHN and PRMT3 . Data are presented as mean fold-change in expression relative to cells transfected with WT 100% used as the calibrator sample (dotted line). Error bars represent the standard error of the mean from three independent experiments.

Article Snippet: Genes previously shown to be regulated by AIRE were analyzed by quantitative PCR using the following primers and probes (Applied Biosystems): S100A8 (Hs0037444264_g1), KRT14 (Hs00265033-m1), IGFL1 (Hs01651089-g1), APOA4 (Hs00166636_m1), and INS (Hs02741908_m1) ( , ).

Techniques: Transfection, Activity Assay, Expressing