tandem mass spectrometry ms ms  (Thermo Fisher)


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    Structured Review

    Thermo Fisher tandem mass spectrometry ms ms
    Hepatic overexpression of Bcl2 disrupts bile acid (BA) homeostasis. ( A ) qPCR and Western blot of liver Bcl2 mRNA and protein, respectively. Bcl2 was expressed in C57BL6 mice by adenovirus mediated gene delivery for two weeks. A second group of mice received 1% cholic acid (CA) feeding for 7 days one week post adenovirus administration. ( B ) Gross morphology of liver, gallbladder, and spleen. ( C ) Serum from each mouse group ( left ) and measurement of serum BA, BA pool size, and fecal BA excretion ( right ). ( D ) BA composition was analyzed using liquid <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry</t> <t>(LC-MS/MS).</t> ( E ) Measurement of serum total bilirubin and direct bilirubin. ( F ) qPCR of genes involved in BA metabolism. Data are represented as mean ± SD in all panels except ( A,B ) * P
    Tandem Mass Spectrometry Ms Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2014 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tandem mass spectrometry ms ms/product/Thermo Fisher
    Average 94 stars, based on 2014 article reviews
    Price from $9.99 to $1999.99
    tandem mass spectrometry ms ms - by Bioz Stars, 2020-09
    94/100 stars

    Images

    1) Product Images from "Bcl2 is a critical regulator of bile acid homeostasis by dictating Shp and lncRNA H19 function"

    Article Title: Bcl2 is a critical regulator of bile acid homeostasis by dictating Shp and lncRNA H19 function

    Journal: Scientific Reports

    doi: 10.1038/srep20559

    Hepatic overexpression of Bcl2 disrupts bile acid (BA) homeostasis. ( A ) qPCR and Western blot of liver Bcl2 mRNA and protein, respectively. Bcl2 was expressed in C57BL6 mice by adenovirus mediated gene delivery for two weeks. A second group of mice received 1% cholic acid (CA) feeding for 7 days one week post adenovirus administration. ( B ) Gross morphology of liver, gallbladder, and spleen. ( C ) Serum from each mouse group ( left ) and measurement of serum BA, BA pool size, and fecal BA excretion ( right ). ( D ) BA composition was analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). ( E ) Measurement of serum total bilirubin and direct bilirubin. ( F ) qPCR of genes involved in BA metabolism. Data are represented as mean ± SD in all panels except ( A,B ) * P
    Figure Legend Snippet: Hepatic overexpression of Bcl2 disrupts bile acid (BA) homeostasis. ( A ) qPCR and Western blot of liver Bcl2 mRNA and protein, respectively. Bcl2 was expressed in C57BL6 mice by adenovirus mediated gene delivery for two weeks. A second group of mice received 1% cholic acid (CA) feeding for 7 days one week post adenovirus administration. ( B ) Gross morphology of liver, gallbladder, and spleen. ( C ) Serum from each mouse group ( left ) and measurement of serum BA, BA pool size, and fecal BA excretion ( right ). ( D ) BA composition was analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). ( E ) Measurement of serum total bilirubin and direct bilirubin. ( F ) qPCR of genes involved in BA metabolism. Data are represented as mean ± SD in all panels except ( A,B ) * P

    Techniques Used: Over Expression, Real-time Polymerase Chain Reaction, Western Blot, Mouse Assay, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Effect of the herbal formulation Jianpijiedu on the TCRVβCDR3 repertoire in rats with hepatocellular carcinoma and subjected to food restriction combined with laxative
    Article Snippet: .. Devices and reagents TRIzol® reagent (15596-026; Thermo Fisher Scientific, Shanghai, China); polymerase chain reaction (PCR) amplification kit (DR011; Takara Biotechnology Co., Ltd., Dalian, China); Hi-Di Formamide (Lot no. 1305031; serial no. 4404307), GeneScan™ 600 LIZ® (Lot no. 1206023; serial no. 4408399) and an Applied Biosystems 3500xL Genetic Analyzer (Thermo Fisher Scientific); and GeneMarker® Genotyping software, version 2.2 (SoftGenetics LLC, State College, PA, USA) were used. ..

    Injection:

    Article Title: Morphology and Blood Metabolites Reflect Recent Spatial Differences Among Lake Winnipeg Walleye, Sander vitreus
    Article Snippet: .. The samples were delivered to the mass spectrometer by a LC method followed by a direct injection (DI) method. .. Data analysis was done using Analyst 1.6.2.

    Amplification:

    Article Title: Effect of the herbal formulation Jianpijiedu on the TCRVβCDR3 repertoire in rats with hepatocellular carcinoma and subjected to food restriction combined with laxative
    Article Snippet: .. Devices and reagents TRIzol® reagent (15596-026; Thermo Fisher Scientific, Shanghai, China); polymerase chain reaction (PCR) amplification kit (DR011; Takara Biotechnology Co., Ltd., Dalian, China); Hi-Di Formamide (Lot no. 1305031; serial no. 4404307), GeneScan™ 600 LIZ® (Lot no. 1206023; serial no. 4408399) and an Applied Biosystems 3500xL Genetic Analyzer (Thermo Fisher Scientific); and GeneMarker® Genotyping software, version 2.2 (SoftGenetics LLC, State College, PA, USA) were used. ..

    Mass Spectrometry:

    Article Title: Morphology and Blood Metabolites Reflect Recent Spatial Differences Among Lake Winnipeg Walleye, Sander vitreus
    Article Snippet: .. The samples were delivered to the mass spectrometer by a LC method followed by a direct injection (DI) method. .. Data analysis was done using Analyst 1.6.2.

    Software:

    Article Title: Effect of the herbal formulation Jianpijiedu on the TCRVβCDR3 repertoire in rats with hepatocellular carcinoma and subjected to food restriction combined with laxative
    Article Snippet: .. Devices and reagents TRIzol® reagent (15596-026; Thermo Fisher Scientific, Shanghai, China); polymerase chain reaction (PCR) amplification kit (DR011; Takara Biotechnology Co., Ltd., Dalian, China); Hi-Di Formamide (Lot no. 1305031; serial no. 4404307), GeneScan™ 600 LIZ® (Lot no. 1206023; serial no. 4408399) and an Applied Biosystems 3500xL Genetic Analyzer (Thermo Fisher Scientific); and GeneMarker® Genotyping software, version 2.2 (SoftGenetics LLC, State College, PA, USA) were used. ..

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    Thermo Fisher api 4000 q trap
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