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Thermo Fisher rsv
Plasma <t>resveratrol</t> <t>(RSV)</t> concentration (ng/mL)–time curve in mice ( n = 3) treated with RSV (blue line) or resveratrol nanoformulation (NP-RSV) (red line) at 4 mg/kg.
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Images

1) Product Images from "Resveratrol and Its Nanoformulation Attenuate Growth and the Angiogenesis of Xenograft and Orthotopic Colon Cancer Models"

Article Title: Resveratrol and Its Nanoformulation Attenuate Growth and the Angiogenesis of Xenograft and Orthotopic Colon Cancer Models

Journal: Molecules

doi: 10.3390/molecules25061412

Plasma resveratrol (RSV) concentration (ng/mL)–time curve in mice ( n = 3) treated with RSV (blue line) or resveratrol nanoformulation (NP-RSV) (red line) at 4 mg/kg.
Figure Legend Snippet: Plasma resveratrol (RSV) concentration (ng/mL)–time curve in mice ( n = 3) treated with RSV (blue line) or resveratrol nanoformulation (NP-RSV) (red line) at 4 mg/kg.

Techniques Used: Concentration Assay, Mouse Assay

Particle size determination of resveratrol nanoformulation (NP-RSV) Using ( A ) transmission electron microscopy (TEM) and ( B ) zeta size analyzer. The average nanoparticle encapsulating RSV ranged from 200–220 nm.
Figure Legend Snippet: Particle size determination of resveratrol nanoformulation (NP-RSV) Using ( A ) transmission electron microscopy (TEM) and ( B ) zeta size analyzer. The average nanoparticle encapsulating RSV ranged from 200–220 nm.

Techniques Used: Transmission Assay, Electron Microscopy, Transmission Electron Microscopy

2) Product Images from "Biotransformation of the chemopreventive agent isoliquiritigenin by UDP-glucuronosyltransferases"

Article Title: Biotransformation of the chemopreventive agent isoliquiritigenin by UDP-glucuronosyltransferases

Journal: Drug metabolism and disposition: the biological fate of chemicals

doi: 10.1124/dmd.108.021857

Negative ion electrospray product ion tandem mass spectra with CID of the deprotonated monoglucuronides A) MG5 of isoliquiritigenin (note that MG3 and MG4 produced identical tandem mass spectra); and B) MG1 of liquiritigenin (note that the tandem mass
Figure Legend Snippet: Negative ion electrospray product ion tandem mass spectra with CID of the deprotonated monoglucuronides A) MG5 of isoliquiritigenin (note that MG3 and MG4 produced identical tandem mass spectra); and B) MG1 of liquiritigenin (note that the tandem mass

Techniques Used: Produced

3) Product Images from "In vivo estrogenic comparisons of Trifolium pratense (red clover) Humulus lupulus (hops), and the pure compounds isoxanthohumol and 8-prenylnaringenin"

Article Title: In vivo estrogenic comparisons of Trifolium pratense (red clover) Humulus lupulus (hops), and the pure compounds isoxanthohumol and 8-prenylnaringenin

Journal: Chemico-biological interactions

doi: 10.1016/j.cbi.2008.06.005

LC-MS-MS chromatograms of 8-PN (dosed at 40 mg/kg/rat) and its metabolites in rat plasma. MRM was carried out using negative ion electrospray and collision-induced dissociation. The precursor and product ion transition for each measurement are indicated
Figure Legend Snippet: LC-MS-MS chromatograms of 8-PN (dosed at 40 mg/kg/rat) and its metabolites in rat plasma. MRM was carried out using negative ion electrospray and collision-induced dissociation. The precursor and product ion transition for each measurement are indicated

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

4) Product Images from "Identification of drugs and drug metabolites as substrates of multidrug resistance protein 2 (MRP2) using triple-transfected MDCK-OATP1B1-UGT1A1-MRP2 cells"

Article Title: Identification of drugs and drug metabolites as substrates of multidrug resistance protein 2 (MRP2) using triple-transfected MDCK-OATP1B1-UGT1A1-MRP2 cells

Journal: British Journal of Pharmacology

doi: 10.1111/j.1476-5381.2011.01672.x

Unlabelled ezetimibe (1 µM) was administered to the basal side of monolayers of MDCK-control (Co), single- (OATP1B1), double- (OATP1B1-UGT1A1, OATP1B1-MRP2) and triple-transfected cells (OATP1B1-UGT1A1-MRP2). Intracellular accumulation of ezetimibe
Figure Legend Snippet: Unlabelled ezetimibe (1 µM) was administered to the basal side of monolayers of MDCK-control (Co), single- (OATP1B1), double- (OATP1B1-UGT1A1, OATP1B1-MRP2) and triple-transfected cells (OATP1B1-UGT1A1-MRP2). Intracellular accumulation of ezetimibe

Techniques Used: Transfection

[ 3 H] Ezetimibe (1 µM) was administered to the basal side of monolayers of MDCK-control (Co), single- (OATP1B1), double- (OATP1B1-UGT1A1, OATP1B1-MRP2) and triple-transfected cells (OATP1B1-UGT1A1-MRP2). Intracellular accumulation of ezetimibe
Figure Legend Snippet: [ 3 H] Ezetimibe (1 µM) was administered to the basal side of monolayers of MDCK-control (Co), single- (OATP1B1), double- (OATP1B1-UGT1A1, OATP1B1-MRP2) and triple-transfected cells (OATP1B1-UGT1A1-MRP2). Intracellular accumulation of ezetimibe

Techniques Used: Transfection

5) Product Images from "Novel IDH1 Mutant Inhibitors for Treatment of Acute Myeloid Leukemia"

Article Title: Novel IDH1 Mutant Inhibitors for Treatment of Acute Myeloid Leukemia

Journal: Nature chemical biology

doi: 10.1038/nchembio.1930

GSK321 decreases intracellular 2-HG and affects proliferation of primary IDH1 mutant AML cells
Figure Legend Snippet: GSK321 decreases intracellular 2-HG and affects proliferation of primary IDH1 mutant AML cells

Techniques Used: Mutagenesis

GSK321 leads to genome-wide DNA cytosine hypomethylation in IDH1-mutant AML cells
Figure Legend Snippet: GSK321 leads to genome-wide DNA cytosine hypomethylation in IDH1-mutant AML cells

Techniques Used: Genome Wide, Mutagenesis

6) Product Images from "Pharmacokinetics of the P-gp Inhibitor Tariquidar in Rats After Intravenous, Oral, and Intraperitoneal Administration"

Article Title: Pharmacokinetics of the P-gp Inhibitor Tariquidar in Rats After Intravenous, Oral, and Intraperitoneal Administration

Journal: European Journal of Drug Metabolism and Pharmacokinetics

doi: 10.1007/s13318-018-0474-x

Concentration–time profiles (mean ± standard deviation) of two tariquidar formulations (A = solution, closed symbols vs. B = microemulsion, open symbols) in plasma of male Sprague–Dawley rats after intravenous ( a ), oral ( b ) and intraperitoneal ( c ) single doses of 15 mg/kg, respectively. For comparison, panel d shows all routes of administration plotted in one single graph
Figure Legend Snippet: Concentration–time profiles (mean ± standard deviation) of two tariquidar formulations (A = solution, closed symbols vs. B = microemulsion, open symbols) in plasma of male Sprague–Dawley rats after intravenous ( a ), oral ( b ) and intraperitoneal ( c ) single doses of 15 mg/kg, respectively. For comparison, panel d shows all routes of administration plotted in one single graph

Techniques Used: Concentration Assay, Standard Deviation

7) Product Images from "In vivo estrogenic comparisons of Trifolium pratense (red clover) Humulus lupulus (hops), and the pure compounds isoxanthohumol and 8-prenylnaringenin"

Article Title: In vivo estrogenic comparisons of Trifolium pratense (red clover) Humulus lupulus (hops), and the pure compounds isoxanthohumol and 8-prenylnaringenin

Journal: Chemico-biological interactions

doi: 10.1016/j.cbi.2008.06.005

LC-MS-MS chromatograms of 8-PN (dosed at 40 mg/kg/rat) and its metabolites in rat plasma. MRM was carried out using negative ion electrospray and collision-induced dissociation. The precursor and product ion transition for each measurement are indicated
Figure Legend Snippet: LC-MS-MS chromatograms of 8-PN (dosed at 40 mg/kg/rat) and its metabolites in rat plasma. MRM was carried out using negative ion electrospray and collision-induced dissociation. The precursor and product ion transition for each measurement are indicated

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

8) Product Images from "Biotransformation of the chemopreventive agent isoliquiritigenin by UDP-glucuronosyltransferases"

Article Title: Biotransformation of the chemopreventive agent isoliquiritigenin by UDP-glucuronosyltransferases

Journal: Drug metabolism and disposition: the biological fate of chemicals

doi: 10.1124/dmd.108.021857

Relative formation of isoliquiritigenin metabolites by recombinant UGT enzymes (0.5 mg protein/mL). In each sample, recombinant UGT was incubated with 10 µM isoliquiritigenin at 37 °C for 20 min in the presence of UDPGA (5 mM). A) MG3
Figure Legend Snippet: Relative formation of isoliquiritigenin metabolites by recombinant UGT enzymes (0.5 mg protein/mL). In each sample, recombinant UGT was incubated with 10 µM isoliquiritigenin at 37 °C for 20 min in the presence of UDPGA (5 mM). A) MG3

Techniques Used: Recombinant, Incubation

Metabolic stability of isoliquiritigenin during phase 2 glucuronidation by pooled human liver microsomes (HLM). Isoliquiritigenin (10 µM) was incubated with microsomes (0.4 mg protein/mL) in the presence of UDPGA (5 mM). The amount of isoliquiritigenin
Figure Legend Snippet: Metabolic stability of isoliquiritigenin during phase 2 glucuronidation by pooled human liver microsomes (HLM). Isoliquiritigenin (10 µM) was incubated with microsomes (0.4 mg protein/mL) in the presence of UDPGA (5 mM). The amount of isoliquiritigenin

Techniques Used: Incubation

Computer-reconstructed mass chromatograms for the negative ion LC-MS analysis of isoliquiritigenin after incubation with pooled human kidney, human intestine, or human liver microsomes (0.5 mg protein/mL) and UDPGA. The metabolites MG3, MG4 and MG5 (also
Figure Legend Snippet: Computer-reconstructed mass chromatograms for the negative ion LC-MS analysis of isoliquiritigenin after incubation with pooled human kidney, human intestine, or human liver microsomes (0.5 mg protein/mL) and UDPGA. The metabolites MG3, MG4 and MG5 (also

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Incubation

Computer-reconstructed mass chromatograms and the total ion chromatogram (TIC) for the negative ion LC-MS analysis of isoliquiritigenin after incubation with human hepatocytes. The metabolites MG3, MG4 and MG5 were identified as isoliquiritigenin 4-,
Figure Legend Snippet: Computer-reconstructed mass chromatograms and the total ion chromatogram (TIC) for the negative ion LC-MS analysis of isoliquiritigenin after incubation with human hepatocytes. The metabolites MG3, MG4 and MG5 were identified as isoliquiritigenin 4-,

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Incubation

Negative ion electrospray product ion tandem mass spectra with CID of the deprotonated monoglucuronides A) MG5 of isoliquiritigenin (note that MG3 and MG4 produced identical tandem mass spectra); and B) MG1 of liquiritigenin (note that the tandem mass
Figure Legend Snippet: Negative ion electrospray product ion tandem mass spectra with CID of the deprotonated monoglucuronides A) MG5 of isoliquiritigenin (note that MG3 and MG4 produced identical tandem mass spectra); and B) MG1 of liquiritigenin (note that the tandem mass

Techniques Used: Produced

UV spectra of isoliquiritigenin and its monoglucuronides. The UV spectra were obtained using a photodiode array detector over the range 210 to 400 nm. Band I (300–380 nm) and Band II (240–280 nm) are associated with the B-ring and A-ring,
Figure Legend Snippet: UV spectra of isoliquiritigenin and its monoglucuronides. The UV spectra were obtained using a photodiode array detector over the range 210 to 400 nm. Band I (300–380 nm) and Band II (240–280 nm) are associated with the B-ring and A-ring,

Techniques Used:

Structures of metabolites and pathways of phase 2 glucuronidation of isoliquiritigenin by human hepatocytes and pooled human liver microsomes in the presence of UDPGA.
Figure Legend Snippet: Structures of metabolites and pathways of phase 2 glucuronidation of isoliquiritigenin by human hepatocytes and pooled human liver microsomes in the presence of UDPGA.

Techniques Used:

9) Product Images from "Resveratrol and Its Nanoformulation Attenuate Growth and the Angiogenesis of Xenograft and Orthotopic Colon Cancer Models"

Article Title: Resveratrol and Its Nanoformulation Attenuate Growth and the Angiogenesis of Xenograft and Orthotopic Colon Cancer Models

Journal: Molecules

doi: 10.3390/molecules25061412

Resveratrol (RSV) and its nanoformulation (NP-RSV) reduced subcutaneous COLO205-luc tumor hemoglobin percentages. Four implants/mouse subcutaneously, in each of the 3 animals per group. * P
Figure Legend Snippet: Resveratrol (RSV) and its nanoformulation (NP-RSV) reduced subcutaneous COLO205-luc tumor hemoglobin percentages. Four implants/mouse subcutaneously, in each of the 3 animals per group. * P

Techniques Used:

Plasma resveratrol (RSV) concentration (ng/mL)–time curve in mice ( n = 3) treated with RSV (blue line) or resveratrol nanoformulation (NP-RSV) (red line) at 4 mg/kg.
Figure Legend Snippet: Plasma resveratrol (RSV) concentration (ng/mL)–time curve in mice ( n = 3) treated with RSV (blue line) or resveratrol nanoformulation (NP-RSV) (red line) at 4 mg/kg.

Techniques Used: Concentration Assay, Mouse Assay

IVIS images (ex vivo) of the orthotopic COLO205-luc tumors after 3 weeks of treatment with resveratrol (RSV) and its nanoformulation (NP-RSV). High signal intensity, red color areas indicate increased viability of cancer cells, while blue color areas indicate the lowest viability or necrosis. A light blue to green color indicates low viability.
Figure Legend Snippet: IVIS images (ex vivo) of the orthotopic COLO205-luc tumors after 3 weeks of treatment with resveratrol (RSV) and its nanoformulation (NP-RSV). High signal intensity, red color areas indicate increased viability of cancer cells, while blue color areas indicate the lowest viability or necrosis. A light blue to green color indicates low viability.

Techniques Used: Ex Vivo

Resveratrol (RSV) and its nanoformulation (NP-RSV) reduced subcutaneous COLO205-luc tumor weight. Four implants/mouse subcutaneously, 3 animals per group. ** P
Figure Legend Snippet: Resveratrol (RSV) and its nanoformulation (NP-RSV) reduced subcutaneous COLO205-luc tumor weight. Four implants/mouse subcutaneously, 3 animals per group. ** P

Techniques Used:

Resveratrol (RSV) and its nanoformulation (NP-RSV) reduced bioluminescent signals of orthotopic colon tumor (COLO205-luc). ** P
Figure Legend Snippet: Resveratrol (RSV) and its nanoformulation (NP-RSV) reduced bioluminescent signals of orthotopic colon tumor (COLO205-luc). ** P

Techniques Used:

Particle size determination of resveratrol nanoformulation (NP-RSV) Using ( A ) transmission electron microscopy (TEM) and ( B ) zeta size analyzer. The average nanoparticle encapsulating RSV ranged from 200–220 nm.
Figure Legend Snippet: Particle size determination of resveratrol nanoformulation (NP-RSV) Using ( A ) transmission electron microscopy (TEM) and ( B ) zeta size analyzer. The average nanoparticle encapsulating RSV ranged from 200–220 nm.

Techniques Used: Transmission Assay, Electron Microscopy, Transmission Electron Microscopy

10) Product Images from "Role of Multidrug Resistance–Associated Protein 4 in the Basolateral Efflux of Hepatically Derived Enalaprilat"

Article Title: Role of Multidrug Resistance–Associated Protein 4 in the Basolateral Efflux of Hepatically Derived Enalaprilat

Journal: Drug Metabolism and Disposition

doi: 10.1124/dmd.114.057554

Transport of enalapril and enalaprilat by MRP3 and MRP4. Uptake of enalapril (100 µ M) (A) and enalaprilat (100 µ M) (B) into membrane vesicles prepared from HEK293T cells expressing MRP3 or MRP4 (10 µ g; solid bars) or mock-transfected
Figure Legend Snippet: Transport of enalapril and enalaprilat by MRP3 and MRP4. Uptake of enalapril (100 µ M) (A) and enalaprilat (100 µ M) (B) into membrane vesicles prepared from HEK293T cells expressing MRP3 or MRP4 (10 µ g; solid bars) or mock-transfected

Techniques Used: Expressing, Transfection

Disposition of taurocholate and rosuvastatin (A) as well as enalapril and derived enalaprilat (B) in human SCH. Accumulation of taurocholate, rosuvastatin, enalapril, and enalaprilat in cells + bile (black bars) and cells (white bars) in human SCH was
Figure Legend Snippet: Disposition of taurocholate and rosuvastatin (A) as well as enalapril and derived enalaprilat (B) in human SCH. Accumulation of taurocholate, rosuvastatin, enalapril, and enalaprilat in cells + bile (black bars) and cells (white bars) in human SCH was

Techniques Used: Derivative Assay

11) Product Images from "Efficacy of NZ2114, a Novel Plectasin-Derived Cationic Antimicrobial Peptide Antibiotic, in Experimental Endocarditis Due to Methicillin-Resistant Staphylococcus aureus ▿"

Article Title: Efficacy of NZ2114, a Novel Plectasin-Derived Cationic Antimicrobial Peptide Antibiotic, in Experimental Endocarditis Due to Methicillin-Resistant Staphylococcus aureus ▿

Journal: Antimicrobial Agents and Chemotherapy

doi: 10.1128/AAC.00453-11

The efficacies of NZ2114, vancomycin, and daptomycin in the IE model.
Figure Legend Snippet: The efficacies of NZ2114, vancomycin, and daptomycin in the IE model.

Techniques Used:

Relationship between the NZ2114 PK/PD indices ( f AUC/MIC, f C max /MIC, and f% T MIC ) and NZ2114's efficacy against MRSA in an experimental IE model. The unbound-drug (free-drug) concentrations were used for index calculations. Efficacy is expressed as the
Figure Legend Snippet: Relationship between the NZ2114 PK/PD indices ( f AUC/MIC, f C max /MIC, and f% T MIC ) and NZ2114's efficacy against MRSA in an experimental IE model. The unbound-drug (free-drug) concentrations were used for index calculations. Efficacy is expressed as the

Techniques Used:

Efficacy of NZ2114, vancomycin (VAN), and daptomycin (DAP) in an experimental IE model. MRSA densities in target tissues after a 3-day NZ2114, VAN, or DAP treatment in the IE model. The values are shown as the mean log 10 numbers of CFU of MRSA/g target
Figure Legend Snippet: Efficacy of NZ2114, vancomycin (VAN), and daptomycin (DAP) in an experimental IE model. MRSA densities in target tissues after a 3-day NZ2114, VAN, or DAP treatment in the IE model. The values are shown as the mean log 10 numbers of CFU of MRSA/g target

Techniques Used:

In vitro time-kill curve of NZ2114. Concentrations equal 0, 0.5×, 1×, and 5× MIC against MRSA ATCC 33591. ⧫, control; ▪, 0.5× MIC of NZ2114; ▴, 1× MIC of NZ2114; and ✖, 5×
Figure Legend Snippet: In vitro time-kill curve of NZ2114. Concentrations equal 0, 0.5×, 1×, and 5× MIC against MRSA ATCC 33591. ⧫, control; ▪, 0.5× MIC of NZ2114; ▴, 1× MIC of NZ2114; and ✖, 5×

Techniques Used: In Vitro

Treatment and relapse comparison of NZ2114, vancomycin, and daptomycin in the IE model. The values shown are log 10 numbers of CFU/g target tissue (MRSA density) ± SD. P was
Figure Legend Snippet: Treatment and relapse comparison of NZ2114, vancomycin, and daptomycin in the IE model. The values shown are log 10 numbers of CFU/g target tissue (MRSA density) ± SD. P was

Techniques Used:

Free-NZ2114 plasma pharmacokinetics after a single i.v. administration. Regimens consisted of a 5-, 10-, or 20-mg/kg bolus in animals with established (24-h) experimental MRSA IE. Each symbol represents the mean of concentrations from three animals. Error
Figure Legend Snippet: Free-NZ2114 plasma pharmacokinetics after a single i.v. administration. Regimens consisted of a 5-, 10-, or 20-mg/kg bolus in animals with established (24-h) experimental MRSA IE. Each symbol represents the mean of concentrations from three animals. Error

Techniques Used:

12) Product Images from "A Semiphysiologically Based Pharmacokinetic Modeling Approach to Predict the Dose-Exposure Relationship of an Antiparasitic Prodrug/Active Metabolite Pair"

Article Title: A Semiphysiologically Based Pharmacokinetic Modeling Approach to Predict the Dose-Exposure Relationship of an Antiparasitic Prodrug/Active Metabolite Pair

Journal: Drug Metabolism and Disposition

doi: 10.1124/dmd.111.040063

Semi-PBPK model-predicted (scheme depicted in B) plasma and liver concentration-time profiles of furamidine in humans after oral administration of 100 mg of pafuramidine free base twice daily (gray lines) and 40 mg of pafuramidine free base once
Figure Legend Snippet: Semi-PBPK model-predicted (scheme depicted in B) plasma and liver concentration-time profiles of furamidine in humans after oral administration of 100 mg of pafuramidine free base twice daily (gray lines) and 40 mg of pafuramidine free base once

Techniques Used: Concentration Assay

); human SCH data were obtained using a similar study design. Pafuramidine
Figure Legend Snippet: ); human SCH data were obtained using a similar study design. Pafuramidine

Techniques Used:

Semi-PBPK model schemes depicting disposition of pafuramidine and furamidine in rat IPLs (A) and in vivo in rats and humans (B). Distinct model structures were developed for the prodrug, pafuramidine, and the derived active metabolite, furamidine, and
Figure Legend Snippet: Semi-PBPK model schemes depicting disposition of pafuramidine and furamidine in rat IPLs (A) and in vivo in rats and humans (B). Distinct model structures were developed for the prodrug, pafuramidine, and the derived active metabolite, furamidine, and

Techniques Used: In Vivo, Derivative Assay

Disposition of Pafuramidine and Furamidine in Rat and Human Sandwich-Cultured Hepatocytes.
Figure Legend Snippet: Disposition of Pafuramidine and Furamidine in Rat and Human Sandwich-Cultured Hepatocytes.

Techniques Used: Cell Culture

Disposition of pafuramidine (♦) and furamidine (▴) in healthy male subjects administered a single oral dose of [ 14 C]pafuramidine maleate [nominal dose 131.9 mg, equivalent to 100 mg (274 μmol) pafuramidine free base] in capsule
Figure Legend Snippet: Disposition of pafuramidine (♦) and furamidine (▴) in healthy male subjects administered a single oral dose of [ 14 C]pafuramidine maleate [nominal dose 131.9 mg, equivalent to 100 mg (274 μmol) pafuramidine free base] in capsule

Techniques Used:

Disposition of pafuramidine (♦) and furamidine (▴) in rats administered a single oral dose of pafuramidine (7.5 μmol/kg). A, comparison of observed (symbols) and semi-PBPK model-predicted (scheme depicted in B) (lines) plasma
Figure Legend Snippet: Disposition of pafuramidine (♦) and furamidine (▴) in rats administered a single oral dose of pafuramidine (7.5 μmol/kg). A, comparison of observed (symbols) and semi-PBPK model-predicted (scheme depicted in B) (lines) plasma

Techniques Used:

13) Product Images from "Role of Multidrug Resistance–Associated Protein 4 in the Basolateral Efflux of Hepatically Derived Enalaprilat"

Article Title: Role of Multidrug Resistance–Associated Protein 4 in the Basolateral Efflux of Hepatically Derived Enalaprilat

Journal: Drug Metabolism and Disposition

doi: 10.1124/dmd.114.057554

Effect of MK-571 on the intrinsic basolateral efflux clearance of enalaprilat in human sandwich-cultured hepatocytes. CL int,basolateral . Clearance values are expressed as a percentage
Figure Legend Snippet: Effect of MK-571 on the intrinsic basolateral efflux clearance of enalaprilat in human sandwich-cultured hepatocytes. CL int,basolateral . Clearance values are expressed as a percentage

Techniques Used: Cell Culture

Transport of enalapril and enalaprilat by MRP3 and MRP4. Uptake of enalapril (100 µ M) (A) and enalaprilat (100 µ M) (B) into membrane vesicles prepared from HEK293T cells expressing MRP3 or MRP4 (10 µ g; solid bars) or mock-transfected
Figure Legend Snippet: Transport of enalapril and enalaprilat by MRP3 and MRP4. Uptake of enalapril (100 µ M) (A) and enalaprilat (100 µ M) (B) into membrane vesicles prepared from HEK293T cells expressing MRP3 or MRP4 (10 µ g; solid bars) or mock-transfected

Techniques Used: Expressing, Transfection

for a detailed description). (B) Average enalaprilat cellular accumulation (red squares) and mass excreted into the efflux buffer (blue
Figure Legend Snippet: for a detailed description). (B) Average enalaprilat cellular accumulation (red squares) and mass excreted into the efflux buffer (blue

Techniques Used:

Effect of MK-571 on MRP4-mediated enalaprilat transport. Membrane vesicles prepared from MRP4-expressing HEK293T cells were incubated with enalaprilat (100 µ M) for 5 minutes with and without MK-571 (50 µ M). MRP4-dependent, ATP-dependent
Figure Legend Snippet: Effect of MK-571 on MRP4-mediated enalaprilat transport. Membrane vesicles prepared from MRP4-expressing HEK293T cells were incubated with enalaprilat (100 µ M) for 5 minutes with and without MK-571 (50 µ M). MRP4-dependent, ATP-dependent

Techniques Used: Expressing, Incubation

Disposition of taurocholate and rosuvastatin (A) as well as enalapril and derived enalaprilat (B) in human SCH. Accumulation of taurocholate, rosuvastatin, enalapril, and enalaprilat in cells + bile (black bars) and cells (white bars) in human SCH was
Figure Legend Snippet: Disposition of taurocholate and rosuvastatin (A) as well as enalapril and derived enalaprilat (B) in human SCH. Accumulation of taurocholate, rosuvastatin, enalapril, and enalaprilat in cells + bile (black bars) and cells (white bars) in human SCH was

Techniques Used: Derivative Assay

14) Product Images from "Novel IDH1 Mutant Inhibitors for Treatment of Acute Myeloid Leukemia"

Article Title: Novel IDH1 Mutant Inhibitors for Treatment of Acute Myeloid Leukemia

Journal: Nature chemical biology

doi: 10.1038/nchembio.1930

GSK321 decreases intracellular 2-HG and affects proliferation of primary IDH1 mutant AML cells
Figure Legend Snippet: GSK321 decreases intracellular 2-HG and affects proliferation of primary IDH1 mutant AML cells

Techniques Used: Mutagenesis

GSK321 leads to genome-wide DNA cytosine hypomethylation in IDH1-mutant AML cells
Figure Legend Snippet: GSK321 leads to genome-wide DNA cytosine hypomethylation in IDH1-mutant AML cells

Techniques Used: Genome Wide, Mutagenesis

Induction of differentiation in primary IDH1 mutant AML blasts and immature stem-like cells
Figure Legend Snippet: Induction of differentiation in primary IDH1 mutant AML blasts and immature stem-like cells

Techniques Used: Mutagenesis

Identification of novel allosteric inhibitors of mutant IDH1
Figure Legend Snippet: Identification of novel allosteric inhibitors of mutant IDH1

Techniques Used: Mutagenesis

15) Product Images from "Soluble epoxide hydrolase limits mechanical hyperalgesia during inflammation"

Article Title: Soluble epoxide hydrolase limits mechanical hyperalgesia during inflammation

Journal: Molecular Pain

doi: 10.1186/1744-8069-7-78

Consequences of sEH deletion on EET and prostaglandin levels in the inflamed paw . (A-C) Changes in EET levels during zymosan induced inflammation. Levels of 8,9- (A), 11,12-(B) and 14,15-EET (C) levels were determined by LC-MS/MS in paw tissues at different time points after zymosan injection. (D) Changes in EET and DHET (E) levels after sEH deletion. Four different regioisomers were determined by LC-MS/MS analysis in paw tissue from wild type and sEH -/- mice 48 h after intraplantar zymosan injection. Data shown represent the mean ± SEM from tissues of 4-5 animals. Student's t test: *, p ≤ 0.05. (F) Effect of sEH deletion on COX-2 expression. COX-2 protein levels were determined by western blot in paw tissues from wild- type and sEH deficient mice 48 h after intraplantar zymosan injection. (G) Effect of sEH deletion on prostaglandin synthesis in the inflamed paw. Prostaglandins were determined 48h after zymosan injection by LC-MS/MS analysis. Data shown represent the mean ± SEM from tissues of 3-5 animals. (H) Effect of sEH deletion on prostaglandin synthesis in the spinal cord. Prostaglandins were determined from lumbal spinal cord tissue 48h after zymosan injection. Data shown represent the mean ± SEM from tissues of 4 animals.
Figure Legend Snippet: Consequences of sEH deletion on EET and prostaglandin levels in the inflamed paw . (A-C) Changes in EET levels during zymosan induced inflammation. Levels of 8,9- (A), 11,12-(B) and 14,15-EET (C) levels were determined by LC-MS/MS in paw tissues at different time points after zymosan injection. (D) Changes in EET and DHET (E) levels after sEH deletion. Four different regioisomers were determined by LC-MS/MS analysis in paw tissue from wild type and sEH -/- mice 48 h after intraplantar zymosan injection. Data shown represent the mean ± SEM from tissues of 4-5 animals. Student's t test: *, p ≤ 0.05. (F) Effect of sEH deletion on COX-2 expression. COX-2 protein levels were determined by western blot in paw tissues from wild- type and sEH deficient mice 48 h after intraplantar zymosan injection. (G) Effect of sEH deletion on prostaglandin synthesis in the inflamed paw. Prostaglandins were determined 48h after zymosan injection by LC-MS/MS analysis. Data shown represent the mean ± SEM from tissues of 3-5 animals. (H) Effect of sEH deletion on prostaglandin synthesis in the spinal cord. Prostaglandins were determined from lumbal spinal cord tissue 48h after zymosan injection. Data shown represent the mean ± SEM from tissues of 4 animals.

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Injection, Mouse Assay, Expressing, Western Blot

16) Product Images from "Biotransformation of the chemopreventive agent isoliquiritigenin by UDP-glucuronosyltransferases"

Article Title: Biotransformation of the chemopreventive agent isoliquiritigenin by UDP-glucuronosyltransferases

Journal: Drug metabolism and disposition: the biological fate of chemicals

doi: 10.1124/dmd.108.021857

Negative ion electrospray product ion tandem mass spectra with CID of the deprotonated monoglucuronides A) MG5 of isoliquiritigenin (note that MG3 and MG4 produced identical tandem mass spectra); and B) MG1 of liquiritigenin (note that the tandem mass
Figure Legend Snippet: Negative ion electrospray product ion tandem mass spectra with CID of the deprotonated monoglucuronides A) MG5 of isoliquiritigenin (note that MG3 and MG4 produced identical tandem mass spectra); and B) MG1 of liquiritigenin (note that the tandem mass

Techniques Used: Produced

UV spectra of isoliquiritigenin and its monoglucuronides. The UV spectra were obtained using a photodiode array detector over the range 210 to 400 nm. Band I (300–380 nm) and Band II (240–280 nm) are associated with the B-ring and A-ring,
Figure Legend Snippet: UV spectra of isoliquiritigenin and its monoglucuronides. The UV spectra were obtained using a photodiode array detector over the range 210 to 400 nm. Band I (300–380 nm) and Band II (240–280 nm) are associated with the B-ring and A-ring,

Techniques Used:

17) Product Images from "Identification of drugs and drug metabolites as substrates of multidrug resistance protein 2 (MRP2) using triple-transfected MDCK-OATP1B1-UGT1A1-MRP2 cells"

Article Title: Identification of drugs and drug metabolites as substrates of multidrug resistance protein 2 (MRP2) using triple-transfected MDCK-OATP1B1-UGT1A1-MRP2 cells

Journal: British Journal of Pharmacology

doi: 10.1111/j.1476-5381.2011.01672.x

Unlabelled ezetimibe (1 µM) was administered to the basal side of monolayers of MDCK-control (Co), single- (OATP1B1), double- (OATP1B1-UGT1A1, OATP1B1-MRP2) and triple-transfected cells (OATP1B1-UGT1A1-MRP2). Intracellular accumulation of ezetimibe
Figure Legend Snippet: Unlabelled ezetimibe (1 µM) was administered to the basal side of monolayers of MDCK-control (Co), single- (OATP1B1), double- (OATP1B1-UGT1A1, OATP1B1-MRP2) and triple-transfected cells (OATP1B1-UGT1A1-MRP2). Intracellular accumulation of ezetimibe

Techniques Used: Transfection

[ 3 H] Ezetimibe (1 µM) was administered to the basal side of monolayers of MDCK-control (Co), single- (OATP1B1), double- (OATP1B1-UGT1A1, OATP1B1-MRP2) and triple-transfected cells (OATP1B1-UGT1A1-MRP2). Intracellular accumulation of ezetimibe
Figure Legend Snippet: [ 3 H] Ezetimibe (1 µM) was administered to the basal side of monolayers of MDCK-control (Co), single- (OATP1B1), double- (OATP1B1-UGT1A1, OATP1B1-MRP2) and triple-transfected cells (OATP1B1-UGT1A1-MRP2). Intracellular accumulation of ezetimibe

Techniques Used: Transfection

18) Product Images from "Translation Control of Swarming Proficiency in Bacillus subtilis by 5-Amino-pentanolylated Elongation Factor P *"

Article Title: Translation Control of Swarming Proficiency in Bacillus subtilis by 5-Amino-pentanolylated Elongation Factor P *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M115.712091

Structural characterization of the B. subtilis EF-P posttranslational modification 5-aminopentanol. A and D , high-resolution mass spectrum of intact recombinant and native Bs EF-P measured on a 7T Fourier transform-ICR. Monoisotopic masses were deconvoluted based on the envelope of multiply charged ions. B and E , chymotrypsin-digested modified and unmodified peptide was sequenced by ETD and revealed Lys 32 to harbor the modification. C and F , ETD-HCD MS 3 of the z5+ ion and proposed charge-directed fragmentation pattern for the modification. Roman numerals and Greek letters represent ion fragments unique to the modification.
Figure Legend Snippet: Structural characterization of the B. subtilis EF-P posttranslational modification 5-aminopentanol. A and D , high-resolution mass spectrum of intact recombinant and native Bs EF-P measured on a 7T Fourier transform-ICR. Monoisotopic masses were deconvoluted based on the envelope of multiply charged ions. B and E , chymotrypsin-digested modified and unmodified peptide was sequenced by ETD and revealed Lys 32 to harbor the modification. C and F , ETD-HCD MS 3 of the z5+ ion and proposed charge-directed fragmentation pattern for the modification. Roman numerals and Greek letters represent ion fragments unique to the modification.

Techniques Used: Modification, Recombinant, Mass Spectrometry

19) Product Images from "Identification of drugs and drug metabolites as substrates of multidrug resistance protein 2 (MRP2) using triple-transfected MDCK-OATP1B1-UGT1A1-MRP2 cells"

Article Title: Identification of drugs and drug metabolites as substrates of multidrug resistance protein 2 (MRP2) using triple-transfected MDCK-OATP1B1-UGT1A1-MRP2 cells

Journal: British Journal of Pharmacology

doi: 10.1111/j.1476-5381.2011.01672.x

Unlabelled ezetimibe (1 µM) was administered to the basal side of monolayers of MDCK-control (Co), single- (OATP1B1), double- (OATP1B1-UGT1A1, OATP1B1-MRP2) and triple-transfected cells (OATP1B1-UGT1A1-MRP2). Intracellular accumulation of ezetimibe
Figure Legend Snippet: Unlabelled ezetimibe (1 µM) was administered to the basal side of monolayers of MDCK-control (Co), single- (OATP1B1), double- (OATP1B1-UGT1A1, OATP1B1-MRP2) and triple-transfected cells (OATP1B1-UGT1A1-MRP2). Intracellular accumulation of ezetimibe

Techniques Used: Transfection

[ 3 H] Ezetimibe (1 µM) was administered to the basal side of monolayers of MDCK-control (Co), single- (OATP1B1), double- (OATP1B1-UGT1A1, OATP1B1-MRP2) and triple-transfected cells (OATP1B1-UGT1A1-MRP2). Intracellular accumulation of ezetimibe
Figure Legend Snippet: [ 3 H] Ezetimibe (1 µM) was administered to the basal side of monolayers of MDCK-control (Co), single- (OATP1B1), double- (OATP1B1-UGT1A1, OATP1B1-MRP2) and triple-transfected cells (OATP1B1-UGT1A1-MRP2). Intracellular accumulation of ezetimibe

Techniques Used: Transfection

20) Product Images from "Novel IDH1 Mutant Inhibitors for Treatment of Acute Myeloid Leukemia"

Article Title: Novel IDH1 Mutant Inhibitors for Treatment of Acute Myeloid Leukemia

Journal: Nature chemical biology

doi: 10.1038/nchembio.1930

GSK321 decreases intracellular 2-HG and affects proliferation of primary IDH1 mutant AML cells
Figure Legend Snippet: GSK321 decreases intracellular 2-HG and affects proliferation of primary IDH1 mutant AML cells

Techniques Used: Mutagenesis

GSK321 leads to genome-wide DNA cytosine hypomethylation in IDH1-mutant AML cells
Figure Legend Snippet: GSK321 leads to genome-wide DNA cytosine hypomethylation in IDH1-mutant AML cells

Techniques Used: Genome Wide, Mutagenesis

Induction of differentiation in primary IDH1 mutant AML blasts and immature stem-like cells
Figure Legend Snippet: Induction of differentiation in primary IDH1 mutant AML blasts and immature stem-like cells

Techniques Used: Mutagenesis

21) Product Images from "Comparison of Liquid Chromatography-Tandem Mass Spectrometry and Sandwich ELISA for Determination of Keratan Sulfate in Plasma and Urine"

Article Title: Comparison of Liquid Chromatography-Tandem Mass Spectrometry and Sandwich ELISA for Determination of Keratan Sulfate in Plasma and Urine

Journal: Biomarker Insights

doi: 10.4137/BMI.S7451

Correlation in KS concentrations. A ) Correlation between plasma and urine KS as measured by LC/MS/MS in MPS IVA patients (n = 36, P = 0.004). B ) Correlation between plasma and urine KS as measured by ELISA in MPS IVA patients (n = 87, P
Figure Legend Snippet: Correlation in KS concentrations. A ) Correlation between plasma and urine KS as measured by LC/MS/MS in MPS IVA patients (n = 36, P = 0.004). B ) Correlation between plasma and urine KS as measured by ELISA in MPS IVA patients (n = 87, P

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Enzyme-linked Immunosorbent Assay

Comparison of plasma and urine KS levels between MPS IVA patients and normal individuals by ELISA and LC/MS/MS methods. A ) Plasma KS results by ELISA. B ) Plasma KS results by LC/MS/MS. C ) Urine KS results by ELISA. D ) Urine KS results by LC/MS/MS. Plasma and urine KS levels by both methods are significantly higher in MPS IVA patients at any age.
Figure Legend Snippet: Comparison of plasma and urine KS levels between MPS IVA patients and normal individuals by ELISA and LC/MS/MS methods. A ) Plasma KS results by ELISA. B ) Plasma KS results by LC/MS/MS. C ) Urine KS results by ELISA. D ) Urine KS results by LC/MS/MS. Plasma and urine KS levels by both methods are significantly higher in MPS IVA patients at any age.

Techniques Used: Enzyme-linked Immunosorbent Assay, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

Concentrations of plasma and urine KS of patients with MPS IVA and normal individuals as measured by ELISA and LC/MS/MS. A ) Plasma KS results by ELISA of 109 specimens from MPS IVA individuals and 212 normal individuals are plotted with respect to age. B ) Urine KS results by ELISA of 100 specimens from MPS IVA individuals and 179 normal individuals are plotted with respect to age. C ) Plasma KS results by LC/MS/MS of 85 specimens from MPS IVA individuals and 165 normal individuals are plotted with respect to age. D ) Urine KS results by LC/MS/MS of 58 specimens from MPS IVA individuals and 134 normal individuals are plotted with respect to age.
Figure Legend Snippet: Concentrations of plasma and urine KS of patients with MPS IVA and normal individuals as measured by ELISA and LC/MS/MS. A ) Plasma KS results by ELISA of 109 specimens from MPS IVA individuals and 212 normal individuals are plotted with respect to age. B ) Urine KS results by ELISA of 100 specimens from MPS IVA individuals and 179 normal individuals are plotted with respect to age. C ) Plasma KS results by LC/MS/MS of 85 specimens from MPS IVA individuals and 165 normal individuals are plotted with respect to age. D ) Urine KS results by LC/MS/MS of 58 specimens from MPS IVA individuals and 134 normal individuals are plotted with respect to age.

Techniques Used: Enzyme-linked Immunosorbent Assay, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

22) Product Images from "A Semiphysiologically Based Pharmacokinetic Modeling Approach to Predict the Dose-Exposure Relationship of an Antiparasitic Prodrug/Active Metabolite Pair"

Article Title: A Semiphysiologically Based Pharmacokinetic Modeling Approach to Predict the Dose-Exposure Relationship of an Antiparasitic Prodrug/Active Metabolite Pair

Journal: Drug Metabolism and Disposition

doi: 10.1124/dmd.111.040063

Semi-PBPK model-predicted (scheme depicted in B) plasma and liver concentration-time profiles of furamidine in humans after oral administration of 100 mg of pafuramidine free base twice daily (gray lines) and 40 mg of pafuramidine free base once
Figure Legend Snippet: Semi-PBPK model-predicted (scheme depicted in B) plasma and liver concentration-time profiles of furamidine in humans after oral administration of 100 mg of pafuramidine free base twice daily (gray lines) and 40 mg of pafuramidine free base once

Techniques Used: Concentration Assay

Semi-PBPK model schemes depicting disposition of pafuramidine and furamidine in rat IPLs (A) and in vivo in rats and humans (B). Distinct model structures were developed for the prodrug, pafuramidine, and the derived active metabolite, furamidine, and
Figure Legend Snippet: Semi-PBPK model schemes depicting disposition of pafuramidine and furamidine in rat IPLs (A) and in vivo in rats and humans (B). Distinct model structures were developed for the prodrug, pafuramidine, and the derived active metabolite, furamidine, and

Techniques Used: In Vivo, Derivative Assay

Disposition of Pafuramidine and Furamidine in Rat and Human Sandwich-Cultured Hepatocytes.
Figure Legend Snippet: Disposition of Pafuramidine and Furamidine in Rat and Human Sandwich-Cultured Hepatocytes.

Techniques Used: Cell Culture

Disposition of pafuramidine (♦) and furamidine (▴) in healthy male subjects administered a single oral dose of [ 14 C]pafuramidine maleate [nominal dose 131.9 mg, equivalent to 100 mg (274 μmol) pafuramidine free base] in capsule
Figure Legend Snippet: Disposition of pafuramidine (♦) and furamidine (▴) in healthy male subjects administered a single oral dose of [ 14 C]pafuramidine maleate [nominal dose 131.9 mg, equivalent to 100 mg (274 μmol) pafuramidine free base] in capsule

Techniques Used:

Disposition of pafuramidine (♦) and furamidine (▴) in rats administered a single oral dose of pafuramidine (7.5 μmol/kg). A, comparison of observed (symbols) and semi-PBPK model-predicted (scheme depicted in B) (lines) plasma
Figure Legend Snippet: Disposition of pafuramidine (♦) and furamidine (▴) in rats administered a single oral dose of pafuramidine (7.5 μmol/kg). A, comparison of observed (symbols) and semi-PBPK model-predicted (scheme depicted in B) (lines) plasma

Techniques Used:

23) Product Images from "Soluble epoxide hydrolase limits mechanical hyperalgesia during inflammation"

Article Title: Soluble epoxide hydrolase limits mechanical hyperalgesia during inflammation

Journal: Molecular Pain

doi: 10.1186/1744-8069-7-78

8,9-EET sensitizes AITC induced TRPA1 response . (A) Representative calcium imaging experiment of 8,9-EET dependent TRPA1-sensitization. DRG cells were stimulated with AITC twice (50 μM, 15 sec) with 10 minutes interval. 1 μM 8,9-EET or vehicle was perfused for two minutes prior to the second AITC stimulation. (B) Statistical analysis comparing the amplitudes of AITC induced TRPA1 response. Data shown represent the mean ± SEM of 21-23 cells. Student's t test: *, p ≤ 0.05 (C) 8,9-EET sensitizes AITC induced CGRP release from sciatic nerve axons. Freshly isolated sciatic nerves were incubated with 10 μM 8,9-EET (ipsilateral) or 3.2% EtOH (vehicle, contralateral) and stimulated with 50 μM AITC. CGRP was determined by ELISA from extracellular solutions. Data shown represent the mean ± SEM from nerves of 3-4 animals. Two way ANOVA with Bonferroni post test: *, p ≤ 0.05.
Figure Legend Snippet: 8,9-EET sensitizes AITC induced TRPA1 response . (A) Representative calcium imaging experiment of 8,9-EET dependent TRPA1-sensitization. DRG cells were stimulated with AITC twice (50 μM, 15 sec) with 10 minutes interval. 1 μM 8,9-EET or vehicle was perfused for two minutes prior to the second AITC stimulation. (B) Statistical analysis comparing the amplitudes of AITC induced TRPA1 response. Data shown represent the mean ± SEM of 21-23 cells. Student's t test: *, p ≤ 0.05 (C) 8,9-EET sensitizes AITC induced CGRP release from sciatic nerve axons. Freshly isolated sciatic nerves were incubated with 10 μM 8,9-EET (ipsilateral) or 3.2% EtOH (vehicle, contralateral) and stimulated with 50 μM AITC. CGRP was determined by ELISA from extracellular solutions. Data shown represent the mean ± SEM from nerves of 3-4 animals. Two way ANOVA with Bonferroni post test: *, p ≤ 0.05.

Techniques Used: Imaging, Size-exclusion Chromatography, Isolation, Incubation, Enzyme-linked Immunosorbent Assay

Consequences of sEH deletion on EET and prostaglandin levels in the inflamed paw . (A-C) Changes in EET levels during zymosan induced inflammation. Levels of 8,9- (A), 11,12-(B) and 14,15-EET (C) levels were determined by LC-MS/MS in paw tissues at different time points after zymosan injection. (D) Changes in EET and DHET (E) levels after sEH deletion. Four different regioisomers were determined by LC-MS/MS analysis in paw tissue from wild type and sEH -/- mice 48 h after intraplantar zymosan injection. Data shown represent the mean ± SEM from tissues of 4-5 animals. Student's t test: *, p ≤ 0.05. (F) Effect of sEH deletion on COX-2 expression. COX-2 protein levels were determined by western blot in paw tissues from wild- type and sEH deficient mice 48 h after intraplantar zymosan injection. (G) Effect of sEH deletion on prostaglandin synthesis in the inflamed paw. Prostaglandins were determined 48h after zymosan injection by LC-MS/MS analysis. Data shown represent the mean ± SEM from tissues of 3-5 animals. (H) Effect of sEH deletion on prostaglandin synthesis in the spinal cord. Prostaglandins were determined from lumbal spinal cord tissue 48h after zymosan injection. Data shown represent the mean ± SEM from tissues of 4 animals.
Figure Legend Snippet: Consequences of sEH deletion on EET and prostaglandin levels in the inflamed paw . (A-C) Changes in EET levels during zymosan induced inflammation. Levels of 8,9- (A), 11,12-(B) and 14,15-EET (C) levels were determined by LC-MS/MS in paw tissues at different time points after zymosan injection. (D) Changes in EET and DHET (E) levels after sEH deletion. Four different regioisomers were determined by LC-MS/MS analysis in paw tissue from wild type and sEH -/- mice 48 h after intraplantar zymosan injection. Data shown represent the mean ± SEM from tissues of 4-5 animals. Student's t test: *, p ≤ 0.05. (F) Effect of sEH deletion on COX-2 expression. COX-2 protein levels were determined by western blot in paw tissues from wild- type and sEH deficient mice 48 h after intraplantar zymosan injection. (G) Effect of sEH deletion on prostaglandin synthesis in the inflamed paw. Prostaglandins were determined 48h after zymosan injection by LC-MS/MS analysis. Data shown represent the mean ± SEM from tissues of 3-5 animals. (H) Effect of sEH deletion on prostaglandin synthesis in the spinal cord. Prostaglandins were determined from lumbal spinal cord tissue 48h after zymosan injection. Data shown represent the mean ± SEM from tissues of 4 animals.

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Injection, Mouse Assay, Expressing, Western Blot

Activation of primary afferent neurons by 8,9-EET . (A) 8,9-EET induces direct calcium influx in sensory neurons. Calcium concentrations were monitored by ratiometric imaging using fura-2 in cultivated neurons from DRGs of adult wild- type mice. Shown is a representative trace. 40 mM KCL solution was used to identify all viable neurons (B). Average values of peak normalized to baseline ratios of experiments shown in panel B. Data shown represent the mean ± SEM from 12 experiments. Student's t test: *, p ≤ 0.05. (C) Phenotypic characterisation of 8,9-EET responsive neurons. Cultivated DRG neurons were stimulated with 10 μM 8,9-EET, 200 μM menthol, 300 nM capsaicin, 100 μM AITC and 40 mM KCL for 10 sec. Representative traces are shown. Relative amount of cells grouped according to their responsiveness to 8,9-EET and TRP-agonists. Percent values were calculated from 280 KCl responsive neurons. Values below the dotted line are the percentage of all 8,9-EET responding cells.
Figure Legend Snippet: Activation of primary afferent neurons by 8,9-EET . (A) 8,9-EET induces direct calcium influx in sensory neurons. Calcium concentrations were monitored by ratiometric imaging using fura-2 in cultivated neurons from DRGs of adult wild- type mice. Shown is a representative trace. 40 mM KCL solution was used to identify all viable neurons (B). Average values of peak normalized to baseline ratios of experiments shown in panel B. Data shown represent the mean ± SEM from 12 experiments. Student's t test: *, p ≤ 0.05. (C) Phenotypic characterisation of 8,9-EET responsive neurons. Cultivated DRG neurons were stimulated with 10 μM 8,9-EET, 200 μM menthol, 300 nM capsaicin, 100 μM AITC and 40 mM KCL for 10 sec. Representative traces are shown. Relative amount of cells grouped according to their responsiveness to 8,9-EET and TRP-agonists. Percent values were calculated from 280 KCl responsive neurons. Values below the dotted line are the percentage of all 8,9-EET responding cells.

Techniques Used: Activation Assay, Imaging, Mouse Assay, Size-exclusion Chromatography

Effects of EETs and sEH deletion on mechanical pain thresholds . (A) 8,9-EET induces mechanical hyperalgesia. 20 μl of 10 μM 8,9-EET was injected intraplantar and mechanical thresholds were determined by the Dynamic Plantar Test. Control animals received vehicle solution containing the corresponding volumes of ethanol (3.2% v/v) and were tested in parallel. Data shown represent the mean ± SEM from 8 animals per group. Two way Anova with Bonferroni post test: *, p ≤ 0.05; **, p ≤ 0.01. (B) Same experiments than in (A) but other EET regioisomers were used. (C) Thermal thresholds after 8,9-EET injection. 20 μl of 10 μM 8,9-EET or vehicle was injected and thermal thresholds were determined by the Hargreaves test. Data shown represent the mean ± SEM from 4 animals. (D) Effect of sEH deletion on mechanical hyperalgesia after zymosan injection. Mechanical thresholds of both hind paws were tested by the dynamic plantar test after unilateral intraplantar zymosan injection and compared between sEH -/- mice and wild type C57BL/6 controls. Data shown represent the mean ± SEM from 8-9 animals per group. Two way ANOVA with Bonferroni post test: **, p ≤ 0.01.
Figure Legend Snippet: Effects of EETs and sEH deletion on mechanical pain thresholds . (A) 8,9-EET induces mechanical hyperalgesia. 20 μl of 10 μM 8,9-EET was injected intraplantar and mechanical thresholds were determined by the Dynamic Plantar Test. Control animals received vehicle solution containing the corresponding volumes of ethanol (3.2% v/v) and were tested in parallel. Data shown represent the mean ± SEM from 8 animals per group. Two way Anova with Bonferroni post test: *, p ≤ 0.05; **, p ≤ 0.01. (B) Same experiments than in (A) but other EET regioisomers were used. (C) Thermal thresholds after 8,9-EET injection. 20 μl of 10 μM 8,9-EET or vehicle was injected and thermal thresholds were determined by the Hargreaves test. Data shown represent the mean ± SEM from 4 animals. (D) Effect of sEH deletion on mechanical hyperalgesia after zymosan injection. Mechanical thresholds of both hind paws were tested by the dynamic plantar test after unilateral intraplantar zymosan injection and compared between sEH -/- mice and wild type C57BL/6 controls. Data shown represent the mean ± SEM from 8-9 animals per group. Two way ANOVA with Bonferroni post test: **, p ≤ 0.01.

Techniques Used: Injection, Mouse Assay

24) Product Images from "An Open, Randomized, Single-Center, Crossover Pharmacokinetic Study of Meropenem after Intraperitoneal and Intravenous Administration in Patients Receiving Automated Peritoneal Dialysis"

Article Title: An Open, Randomized, Single-Center, Crossover Pharmacokinetic Study of Meropenem after Intraperitoneal and Intravenous Administration in Patients Receiving Automated Peritoneal Dialysis

Journal: Antimicrobial Agents and Chemotherapy

doi: 10.1128/AAC.02664-15

Meropenem serum and dialysate concentrations after intravenous and intraperitoneal administration shown for individual patients. Consider the different scales on the y axes. Vertical arrows indicate the start of PD cycles. Pat, patient.
Figure Legend Snippet: Meropenem serum and dialysate concentrations after intravenous and intraperitoneal administration shown for individual patients. Consider the different scales on the y axes. Vertical arrows indicate the start of PD cycles. Pat, patient.

Techniques Used:

Mean (±standard deviation) serum and dialysate concentrations of meropenem after intravenous and intraperitoneal administration. The vertical line indicates the start of PD cycles.
Figure Legend Snippet: Mean (±standard deviation) serum and dialysate concentrations of meropenem after intravenous and intraperitoneal administration. The vertical line indicates the start of PD cycles.

Techniques Used: Standard Deviation

25) Product Images from "Soluble epoxide hydrolase limits mechanical hyperalgesia during inflammation"

Article Title: Soluble epoxide hydrolase limits mechanical hyperalgesia during inflammation

Journal: Molecular Pain

doi: 10.1186/1744-8069-7-78

Consequences of sEH deletion on EET and prostaglandin levels in the inflamed paw . (A-C) Changes in EET levels during zymosan induced inflammation. Levels of 8,9- (A), 11,12-(B) and 14,15-EET (C) levels were determined by LC-MS/MS in paw tissues at different time points after zymosan injection. (D) Changes in EET and DHET (E) levels after sEH deletion. Four different regioisomers were determined by LC-MS/MS analysis in paw tissue from wild type and sEH -/- mice 48 h after intraplantar zymosan injection. Data shown represent the mean ± SEM from tissues of 4-5 animals. Student's t test: *, p ≤ 0.05. (F) Effect of sEH deletion on COX-2 expression. COX-2 protein levels were determined by western blot in paw tissues from wild- type and sEH deficient mice 48 h after intraplantar zymosan injection. (G) Effect of sEH deletion on prostaglandin synthesis in the inflamed paw. Prostaglandins were determined 48h after zymosan injection by LC-MS/MS analysis. Data shown represent the mean ± SEM from tissues of 3-5 animals. (H) Effect of sEH deletion on prostaglandin synthesis in the spinal cord. Prostaglandins were determined from lumbal spinal cord tissue 48h after zymosan injection. Data shown represent the mean ± SEM from tissues of 4 animals.
Figure Legend Snippet: Consequences of sEH deletion on EET and prostaglandin levels in the inflamed paw . (A-C) Changes in EET levels during zymosan induced inflammation. Levels of 8,9- (A), 11,12-(B) and 14,15-EET (C) levels were determined by LC-MS/MS in paw tissues at different time points after zymosan injection. (D) Changes in EET and DHET (E) levels after sEH deletion. Four different regioisomers were determined by LC-MS/MS analysis in paw tissue from wild type and sEH -/- mice 48 h after intraplantar zymosan injection. Data shown represent the mean ± SEM from tissues of 4-5 animals. Student's t test: *, p ≤ 0.05. (F) Effect of sEH deletion on COX-2 expression. COX-2 protein levels were determined by western blot in paw tissues from wild- type and sEH deficient mice 48 h after intraplantar zymosan injection. (G) Effect of sEH deletion on prostaglandin synthesis in the inflamed paw. Prostaglandins were determined 48h after zymosan injection by LC-MS/MS analysis. Data shown represent the mean ± SEM from tissues of 3-5 animals. (H) Effect of sEH deletion on prostaglandin synthesis in the spinal cord. Prostaglandins were determined from lumbal spinal cord tissue 48h after zymosan injection. Data shown represent the mean ± SEM from tissues of 4 animals.

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Injection, Mouse Assay, Expressing, Western Blot

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Article Snippet: .. Groups ( n = 6) of 6-week-old Golden Syrian hamsters (Envigo Laboratories), confirmed to be seronegative for RSV and HPIV3 by plaque reduction neutralization assay using RSV expressing green fluorescent protein (GFP) and hemagglutination inhibition (HAI) assay, respectively, were anesthetized and intranasally (i.n.) inoculated with 105 TCID50 of rB/HPIV3 vectors or 106 PFU of wt RSV in 0.1 ml of Leibovitz's L15 medium (Thermo Fisher Scientific). ..

Expressing:

Article Title: Improved Prefusion Stability, Optimized Codon Usage, and Augmented Virion Packaging Enhance the Immunogenicity of Respiratory Syncytial Virus Fusion Protein in a Vectored-Vaccine Candidate
Article Snippet: .. Groups ( n = 6) of 6-week-old Golden Syrian hamsters (Envigo Laboratories), confirmed to be seronegative for RSV and HPIV3 by plaque reduction neutralization assay using RSV expressing green fluorescent protein (GFP) and hemagglutination inhibition (HAI) assay, respectively, were anesthetized and intranasally (i.n.) inoculated with 105 TCID50 of rB/HPIV3 vectors or 106 PFU of wt RSV in 0.1 ml of Leibovitz's L15 medium (Thermo Fisher Scientific). ..

Neutralization:

Article Title: Improved Prefusion Stability, Optimized Codon Usage, and Augmented Virion Packaging Enhance the Immunogenicity of Respiratory Syncytial Virus Fusion Protein in a Vectored-Vaccine Candidate
Article Snippet: .. Groups ( n = 6) of 6-week-old Golden Syrian hamsters (Envigo Laboratories), confirmed to be seronegative for RSV and HPIV3 by plaque reduction neutralization assay using RSV expressing green fluorescent protein (GFP) and hemagglutination inhibition (HAI) assay, respectively, were anesthetized and intranasally (i.n.) inoculated with 105 TCID50 of rB/HPIV3 vectors or 106 PFU of wt RSV in 0.1 ml of Leibovitz's L15 medium (Thermo Fisher Scientific). ..

HAI Assay:

Article Title: Improved Prefusion Stability, Optimized Codon Usage, and Augmented Virion Packaging Enhance the Immunogenicity of Respiratory Syncytial Virus Fusion Protein in a Vectored-Vaccine Candidate
Article Snippet: .. Groups ( n = 6) of 6-week-old Golden Syrian hamsters (Envigo Laboratories), confirmed to be seronegative for RSV and HPIV3 by plaque reduction neutralization assay using RSV expressing green fluorescent protein (GFP) and hemagglutination inhibition (HAI) assay, respectively, were anesthetized and intranasally (i.n.) inoculated with 105 TCID50 of rB/HPIV3 vectors or 106 PFU of wt RSV in 0.1 ml of Leibovitz's L15 medium (Thermo Fisher Scientific). ..

Infection:

Article Title: TLR2/MyD88/NF-?B Pathway, Reactive Oxygen Species, Potassium Efflux Activates NLRP3/ASC Inflammasome during Respiratory Syncytial Virus Infection
Article Snippet: .. Viral infection of cells 293 cells, U937 cells and BMDM were infected with purified RSV at 1 multiplicity of infection (MOI) in serum free antibiotic free OPTI-MEM medium (GIBCO). .. Virus adsorption was performed for 1.5 h at 37°C.

Purification:

Article Title: TLR2/MyD88/NF-?B Pathway, Reactive Oxygen Species, Potassium Efflux Activates NLRP3/ASC Inflammasome during Respiratory Syncytial Virus Infection
Article Snippet: .. Viral infection of cells 293 cells, U937 cells and BMDM were infected with purified RSV at 1 multiplicity of infection (MOI) in serum free antibiotic free OPTI-MEM medium (GIBCO). .. Virus adsorption was performed for 1.5 h at 37°C.

Concentration Assay:

Article Title: P-glycoprotein mediated interaction between (R)-[11C]verapamil and tariquidar at the human blood-brain barrier studied with positron emission tomography, a comparison with rat data
Article Snippet: .. The concentration of tariquidar in plasma was measured using a Dionex UltiMate 3000 system (Dionex Corp., Sunnyvale, CA, USA) connected with an API 4000 triple-quadrupole mass spectrometer (Applied Biosystems, Concord, Ontario, Canada). .. After addition of 200 μL of methanol to 100 μL of plasma, the samples were centrifuged (5,000 g for 5 min at 4°C) and 80 μL of the clear supernatant was injected onto the HPLC column.

Incubation:

Article Title: NPC1L1 inhibition disturbs lipid trafficking and induces large lipid droplet formation in intestinal absorptive epithelial cells
Article Snippet: .. The cells were treated with or without 50 μM ezetimibe for 2 h, lipid micelles were added to the apical media, and the cells were incubated for 2 h. Then, the cells were depleted of methionine and cysteine for 30 min by placing in methionine- and cysteine-free medium (methionine cysteine free DMEM, Cat. No. 21013-024, Life Technologies) with or without 50 μM ezetimibe. .. Then, newly synthesized proteins in the cells were labeled by treating with 150 μCi/ml of 35 S-methionine/cysteine (EXPRE35 S35 S Protein Labeling Mix, NEG772, PerkinElmer) apically for 45 min (pulse labeling).

Activity Assay:

Article Title: Resveratrol, a multitargeted agent, can enhance antitumor activity of gemcitabine in vitro and in orthotopic mouse model of human pancreatic cancer
Article Snippet: .. To determine whether resveratrol can potentiate the apoptotic effects of gemcitabine in pancreatic cancer cells, we used a Live/Dead assay kit (Molecular Probes, Eugene, OR), which determines intracellular esterase activity and plasma membrane integrity. .. Briefly, cells (5,000 per well) were incubated in a chamber slide, pretreated with resveratrol for 4 hr, and then treated with gemcitabine for 24 hr.

Mass Spectrometry:

Article Title: P-glycoprotein mediated interaction between (R)-[11C]verapamil and tariquidar at the human blood-brain barrier studied with positron emission tomography, a comparison with rat data
Article Snippet: .. The concentration of tariquidar in plasma was measured using a Dionex UltiMate 3000 system (Dionex Corp., Sunnyvale, CA, USA) connected with an API 4000 triple-quadrupole mass spectrometer (Applied Biosystems, Concord, Ontario, Canada). .. After addition of 200 μL of methanol to 100 μL of plasma, the samples were centrifuged (5,000 g for 5 min at 4°C) and 80 μL of the clear supernatant was injected onto the HPLC column.

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    Thermo Fisher api 4000 q trap
    Api 4000 Q Trap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 663 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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