apha3 cassette  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs apha3 cassette
    Apha3 Cassette, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apha3 cassette/product/New England Biolabs
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    apha3 cassette - by Bioz Stars, 2020-04
    95/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Requirement of NMB0065 for connecting assembly and export of sialic acid capsular polysaccharides in Neisseria meningitidis
    Article Snippet: The resulting PCR fragment was cloned into pCR2.1 (Invitrogen) and sub-cloned into pUC19 using KpnI/XbaI digestion to yield pRH5. .. A SmaI fragment of pUC18K containing the aphA3 cassette [ ] was inserted into the unique BstBI site of pRH5 blunted with Klenow DNA polymerase (New England Biolabs) to yield pRH6 with the aphA3 cassette inserted at 285 bp of the 927-bp NMB0065 coding sequence. pRH6 was used to transform wild-type NMB cells and kanamycin-resistant colonies were selected.

    Amplification:

    Article Title: Requirement of NMB0065 for connecting assembly and export of sialic acid capsular polysaccharides in Neisseria meningitidis
    Article Snippet: An internal coding region was amplified by PCR with primers RH014 (5′-CAATATTATGACGCAGTAATTTTATCGG-3′) and RH019 (5′-CCGGATTTGCTATTTTTGGG-3′) with NMB chromosomal DNA as the template. .. A SmaI fragment of pUC18K containing the aphA3 cassette [ ] was inserted into the unique BstBI site of pRH5 blunted with Klenow DNA polymerase (New England Biolabs) to yield pRH6 with the aphA3 cassette inserted at 285 bp of the 927-bp NMB0065 coding sequence. pRH6 was used to transform wild-type NMB cells and kanamycin-resistant colonies were selected.

    Mutagenesis:

    Article Title: Requirement of NMB0065 for connecting assembly and export of sialic acid capsular polysaccharides in Neisseria meningitidis
    Article Snippet: A SmaI fragment of pUC18K containing the aphA3 cassette [ ] was inserted into the unique BstBI site of pRH5 blunted with Klenow DNA polymerase (New England Biolabs) to yield pRH6 with the aphA3 cassette inserted at 285 bp of the 927-bp NMB0065 coding sequence. pRH6 was used to transform wild-type NMB cells and kanamycin-resistant colonies were selected. .. The mutation was confirmed by PCR using an aphA3 -specific primer (KanA: 5′-CTTAGCAGGAGACATTCCTTCCG - 3′) and a NMB0065-specific primer (RH035: 5′-CTTTGATAGATTGATAATAATGGTTG - 3′).

    Construct:

    Article Title: Requirement of NMB0065 for connecting assembly and export of sialic acid capsular polysaccharides in Neisseria meningitidis
    Article Snippet: A SmaI fragment of pUC18K containing the aphA3 cassette [ ] was inserted into the unique BstBI site of pRH5 blunted with Klenow DNA polymerase (New England Biolabs) to yield pRH6 with the aphA3 cassette inserted at 285 bp of the 927-bp NMB0065 coding sequence. pRH6 was used to transform wild-type NMB cells and kanamycin-resistant colonies were selected. .. Similar mutations were also constructed in the serogroups C and Y strains.

    Sequencing:

    Article Title: Requirement of NMB0065 for connecting assembly and export of sialic acid capsular polysaccharides in Neisseria meningitidis
    Article Snippet: .. A SmaI fragment of pUC18K containing the aphA3 cassette [ ] was inserted into the unique BstBI site of pRH5 blunted with Klenow DNA polymerase (New England Biolabs) to yield pRH6 with the aphA3 cassette inserted at 285 bp of the 927-bp NMB0065 coding sequence. pRH6 was used to transform wild-type NMB cells and kanamycin-resistant colonies were selected. .. The mutation was confirmed by PCR using an aphA3 -specific primer (KanA: 5′-CTTAGCAGGAGACATTCCTTCCG - 3′) and a NMB0065-specific primer (RH035: 5′-CTTTGATAGATTGATAATAATGGTTG - 3′).

    Polymerase Chain Reaction:

    Article Title: Requirement of NMB0065 for connecting assembly and export of sialic acid capsular polysaccharides in Neisseria meningitidis
    Article Snippet: The resulting PCR fragment was cloned into pCR2.1 (Invitrogen) and sub-cloned into pUC19 using KpnI/XbaI digestion to yield pRH5. .. A SmaI fragment of pUC18K containing the aphA3 cassette [ ] was inserted into the unique BstBI site of pRH5 blunted with Klenow DNA polymerase (New England Biolabs) to yield pRH6 with the aphA3 cassette inserted at 285 bp of the 927-bp NMB0065 coding sequence. pRH6 was used to transform wild-type NMB cells and kanamycin-resistant colonies were selected.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    New England Biolabs apha3 cassette
    Apha3 Cassette, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apha3 cassette/product/New England Biolabs
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    apha3 cassette - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    Image Search Results