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anti hcn3  (Alomone Labs)


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    Alomone Labs anti hcn3
    Anti Hcn3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apc-057/pmc12471236-134-13-18?v=Alomone+Labs
    Average 93 stars, based on 28 article reviews
    anti hcn3 - by Bioz Stars, 2026-07
    93/100 stars

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    (A) Peripheral blood mononuclear cells were collected and t otal circulating B c ells were purified. These were incubated with supplements, cytokines and stimulatory and co-stimulatory signalling elements to generate long-lived plasma cells. Created with Biorender.com ( B-C ) Flow cytometry demonstrating plasma cell differentiation of B cells from: ( B ) total B cells on day 0 to ( C ) plasma cells on day 13 in culture using staining with antibodies against CD38 and CD138. The results show the fluorescence intensities in representative samples.

    Journal: medRxiv

    Article Title: Effects of cladribine on intrathecal and peripheral B and plasma cells

    doi: 10.1101/2024.09.19.24313610

    Figure Lengend Snippet: (A) Peripheral blood mononuclear cells were collected and t otal circulating B c ells were purified. These were incubated with supplements, cytokines and stimulatory and co-stimulatory signalling elements to generate long-lived plasma cells. Created with Biorender.com ( B-C ) Flow cytometry demonstrating plasma cell differentiation of B cells from: ( B ) total B cells on day 0 to ( C ) plasma cells on day 13 in culture using staining with antibodies against CD38 and CD138. The results show the fluorescence intensities in representative samples.

    Article Snippet: Antibodies used were: CD19-PE (LT19), CD138-APC (44F9) (Miltenyi); CD20-e450 (2H7) (eBioscience); CD27-FITC (M-T271), CD38-PECy7 (HB7).

    Techniques: Purification, Incubation, Clinical Proteomics, Flow Cytometry, Cell Differentiation, Staining, Fluorescence

    Microarray analysis of B cell subsets from (A-E) ex vivo flow cytometry sorted cells, (F-J) in vitro differentiated plasma cells derived from total B cells using (A-E) Affymetrix human genome U133 plus 2.0 Array or the (F-J) Illumina Human-HT V4.0 expression bead chip detecting: deoxycytidine kinase (DCK. Probes 203302_at and ILMN_1651433), membrane spanning four A one (CD20. Probes 210356_x. ILMN_1776939), TNFRSF7 (CD27 probes 206150_at, ILMN _1688959), (CD38. Probes 205692_at, ILMN_2233783), Syndecan-one (CD138. Probes 20186_at, ILMN_1815303). Data was extracted from www.genomicscape.com database and the results represent the mean + standard deviation mRNA expression (arbitrary units (a.u.)) from (A-E) n=5/group or (F-J) n=3/group.

    Journal: medRxiv

    Article Title: Effects of cladribine on intrathecal and peripheral B and plasma cells

    doi: 10.1101/2024.09.19.24313610

    Figure Lengend Snippet: Microarray analysis of B cell subsets from (A-E) ex vivo flow cytometry sorted cells, (F-J) in vitro differentiated plasma cells derived from total B cells using (A-E) Affymetrix human genome U133 plus 2.0 Array or the (F-J) Illumina Human-HT V4.0 expression bead chip detecting: deoxycytidine kinase (DCK. Probes 203302_at and ILMN_1651433), membrane spanning four A one (CD20. Probes 210356_x. ILMN_1776939), TNFRSF7 (CD27 probes 206150_at, ILMN _1688959), (CD38. Probes 205692_at, ILMN_2233783), Syndecan-one (CD138. Probes 20186_at, ILMN_1815303). Data was extracted from www.genomicscape.com database and the results represent the mean + standard deviation mRNA expression (arbitrary units (a.u.)) from (A-E) n=5/group or (F-J) n=3/group.

    Article Snippet: Antibodies used were: CD19-PE (LT19), CD138-APC (44F9) (Miltenyi); CD20-e450 (2H7) (eBioscience); CD27-FITC (M-T271), CD38-PECy7 (HB7).

    Techniques: Microarray, Ex Vivo, Flow Cytometry, In Vitro, Clinical Proteomics, Derivative Assay, Expressing, Membrane, Standard Deviation