Journal: Hypertension (Dallas, Tex. : 1979)
Article Title: Bradykinin stimulates renal Na + and K + excretion by inhibiting the K + channel (Kir4.1) in the distal convoluted tubule
doi: 10.1161/HYPERTENSIONAHA.118.11070
Figure Lengend Snippet: A double staining image shows the expression of Kir4.1 (brown) and BK2R (blue) with low magnification (A). Areas marked by two squares (B) are enlarged, demonstrating detailed view of BK2R staining in Fig. 1C and 1D, respectively. The distal convoluted tubules (DCT) are indicated by arrows. A red arrow indicates BK2R staining in the lateral membrane of the DCT.
Article Snippet: Briefly, the first antibody immunostaining (Kir4.1) (1:1200, Alomone Labs, Israel) was developed with diaminobenzidine-hydrogen peroxide to give a brown color, whereas BK2R immunostaining was developed with Vector SG substrate to give a blue color (1:1000, Santa Cruz).
Techniques: Double Staining, Expressing, Staining