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cd152 ctla 4 apc  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd152 ctla 4 apc
    Cd152 Ctla 4 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apc-016/CD152+Antibody%2C+anti-human/pmc13053985-9-0-7
    Average 93 stars, based on 117 article reviews
    cd152 ctla 4 apc - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: DNAM-1 mediates NK-cell activation and host-pathogen interaction via direct binding to fungal cell wall proteases.
    Article Snippet: Thereafter, cells were washed with 2 ml HBSS supplemented with 2 mM EDTA and 1% FBS, resuspended in 150 μl of 4% paraformaldehyde (Sigma-Aldrich, #47608), and incubated for 30 minutes at RT. .. AR TI CL E IN P RE SS For the analysis of surface expression in ΔDNAM-1 NK cells and proof of DNAM-1 deletion, cells were treated with anti-CD56 FITC and anti-CD226 APC antibodies (Miltenyi Biotech, #130-114-549 and #130-117-490). ..

    Article Title: DNAM-1 mediates NK-cell activation and host-pathogen interaction via direct binding to fungal cell wall proteases
    Article Snippet: Thereafter, cells were washed with 2 ml HBSS supplemented with 2 mM EDTA and 1% FBS, resuspended in 150 μl of 4% paraformaldehyde (Sigma-Aldrich, #47608), and incubated for 30 min at RT. .. For analysis of surface expression in ΔDNAM-1 NK cells and proof of DNAM-1 deletion, cells were treated with anti-CD56 FITC and anti-CD226 APC antibodies (Miltenyi Biotech, #130-114-549 and #130-117-490). ..

    Staining:

    Article Title: CRISPR/Cas9-mediated t(4;11) translocation in human hematopoietic stem/precursor cells demonstrates plasticity to differentiate into either the myeloid or lymphoid lineage
    Article Snippet: The samples were measured with the BD FACSVerseTM instrument and analyzed with the FlowJo software. .. The following antibodies have been used: anti-human CD45-FITC (clone REA747; Miltenyi; #130-110-769), anti-human CD33-PE-Vio770 (clone REA775; Miltenyi; #130-111-138), anti-human CD123-APC-Vio770 (clone AC145; Miltenyi; #130-113-885), anti-human CD56-APC (clone REA196; Miltenyi; #130-113-872), anti-human CD19-BV510 (clone HIB19; BD Biosciences; #740164), anti-human CD20-V450 (clone L27; BD Biosciences; #561164), anti-human lineage cocktail 1(lin 1) [anti-human CD3-FITC (clone SK7), anti-human CD14-FITC (clone 3G8), anti-human CD16-FITC (clone SJ25C1), anti-human CD19-FITC (clone L27), anti-human CD20-FITC (clone MφP9), anti-human CD56-FITC (clone NCAM16.2)] (BD Biosciences; #340546), anti-human CD10-APC-Vio770 (clone 97C5; Miltenyi; #130-128-300), anti-human CD90-APC-Vio770 (clone REA879; Miltenyi; #130-114-905), anti-human CD45RA-BV-510 (clone HI100; BD Biosciences; #563031) and the viability stain 7-AAD (Miltenyi; #130-111-568). .. The CellTraceTM Violet Cell Proliferation Kit (Thermo Scientific; # C34557 ) was used to track cell proliferation and cell division.

    Article Title: CRISPR/Cas9-mediated t(4;11) translocation in human hematopoietic stem/precursor cells demonstrates plasticity to differentiate into either the myeloid or lymphoid lineage.
    Article Snippet: The samples were measured with the BD FACSVerseTM instrument and analyzed with the FlowJo software. .. The following antibodies have been used: antihuman CD45-FITC (clone REA747; Miltenyi; #130-110-769), anti-human CD33-PE-Vio770 (clone REA775; Miltenyi; #130-111-138), anti-human CD123-APC-Vio770 (clone AC145; Miltenyi; #130-113-885), anti-human CD56-APC (clone REA196; Miltenyi; #130-113-872), anti-human CD19BV510 (clone HIB19; BD Biosciences; #740164), anti-human CD20-V450 (clone L27; BD Biosciences; #561164), anti-human lineage cocktail 1(lin 1) [anti-human CD3-FITC (clone SK7), anti-human CD14-FITC (clone 3G8), antihuman CD16-FITC (clone SJ25C1), anti-human CD19-FITC (clone L27), antihuman CD20-FITC (clone MφP9), anti-human CD56-FITC (clone NCAM16.2)] (BD Biosciences; #340546), anti-human CD10-APC-Vio770 (clone 97C5; Miltenyi; #130-128-300), anti-human CD90-APC-Vio770 (clone REA879; Miltenyi; #130-114-905), anti-human CD45RA-BV-510 (clone HI100; BD Biosciences; #563031) and the viability stain 7-AAD (Miltenyi; #130-111- 568). .. Cell trace proliferation assay The CellTraceTM Violet Cell Proliferation Kit (Thermo Scientific; #C34557) was used to track cell proliferation and cell division.

    other:

    Article Title: Protocol to isolate, expand, and activate human type 2 innate lymphoid cells to study immune checkpoint function
    Article Snippet: CD152 (CTLA-4)-APC (clone: BNI3) (diluted 1:20) , Miltenyi , Cat#130-123-812; RRID: AB_2857639.


    Isolation:

    Article Title: Nanobody-based canine PD-L1-targeting immune checkpoint inhibitors for cancer therapy in dogs
    Article Snippet: Cells were stored in RPMI 1640 medium (Gibco, Life Technologies, Carlsbad, CA, USA) complemented with 10% fetal bovine serum (FBS, Gibco, Life Technologies, Carlsbad, CA, USA) at 37 °C and used in the following 24 h. The purity of the NK cells was determined by flow cytometry. .. Isolated primary NK Cells were plated at 200’000 cells per well in 96-well plates and incubated with phenotyping antibodies: anti-CD3-vioblue (cat. 130-094-363), anti-CD56-APC (cat. 130-113-310) and anti-CD16-FITC (cat. 130-113-392) (all from Miltenyi Biotec, Bergisch Gladbach, Germany). .. After washing, cells were analyzed using a MACSQuant X Fast cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany) and affinity was measured as median of fluorescence (MFI).

    Incubation:

    Article Title: Nanobody-based canine PD-L1-targeting immune checkpoint inhibitors for cancer therapy in dogs
    Article Snippet: Cells were stored in RPMI 1640 medium (Gibco, Life Technologies, Carlsbad, CA, USA) complemented with 10% fetal bovine serum (FBS, Gibco, Life Technologies, Carlsbad, CA, USA) at 37 °C and used in the following 24 h. The purity of the NK cells was determined by flow cytometry. .. Isolated primary NK Cells were plated at 200’000 cells per well in 96-well plates and incubated with phenotyping antibodies: anti-CD3-vioblue (cat. 130-094-363), anti-CD56-APC (cat. 130-113-310) and anti-CD16-FITC (cat. 130-113-392) (all from Miltenyi Biotec, Bergisch Gladbach, Germany). .. After washing, cells were analyzed using a MACSQuant X Fast cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany) and affinity was measured as median of fluorescence (MFI).



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