Journal: bioRxiv
Article Title: Disrupted stemness and redox homeostasis in mesenchymal stem cells of neonates from mothers with obesity: implications for increased adiposity
doi: 10.1101/2025.04.14.648714
Figure Lengend Snippet: A . Cells were positive for surface markers CD73, CD90, and CD105, while negative to CD34, CD45 and CD11b in NW-MSCs and OB-MSCs (n=3). The gating of cells is plotted as forward scatter versus side scatter for cell population and forward scatter versus forward scatter for singlets (top). Cell populations CD73+ and CD90+, CD105+ and CD34-, and CD45- and CD11b-(bottom). Representative graphs show surface markers of NW-MSCs. B. Cells were cultured in three different induction media (osteogenic and myogenic) for 14 days, and in adipogenic induction medium for 21 days. Cells were stained for lipids (Oil Red O), calcium (Alizarin Red) and α-SMA (phalloidin) (n=3). Representative images show induction of NW-MSCs (Scale bars Oil Red O and Alizarin Red: 100 μm; Phalloidin: 400 μm).
Article Snippet: After this, 1×10 6 cells were resuspended in 100 μl of FACS buffer (2% FCS in DPBS) in FACS tubes (Falcon, Corning) and incubated for 30 minutes in the dark with antibodies for CD73 (APC, 1:50, Miltenyi Biotec), CD90 (PE, 1:50, Miltenyi Biotec), CD105 (BV421, 1:20, BioLegend), CD34 (PE-Dazzle 594, 1:20, Biolegend), CD45 (BV785, 1:20, BioLegend), and CD11b (APC-Vio770, 1:50, Miltenyi Biotec).
Techniques: Cell Culture, Staining