apal1 restriction enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs apal1 restriction enzyme
    Apal1 Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apal1 restriction enzyme/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apal1 restriction enzyme - by Bioz Stars, 2021-07
    86/100 stars

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    Fluorescence:

    Article Title: The histone demethylase LSD1 regulates inner ear progenitor differentiation through interactions with Pax2 and the NuRD repressor complex
    Article Snippet: To induce differentiation, N33 cells were cultured in equal mixture of Neurobasal (Gibco) and Advance DMEM/F12 medium (Gibco) supplemented with 100 U/ml penicillin-streptomycin, 1% Glutamax (Gibco), 1% N-2 Supplement (Gibco) and 2% B27 Supplement (Gibco) in 5% CO2 /95% air at 39°C. .. To make stable cells, the PRS4-enhanced green fluorescence protein (EGFP) construct containing the neo gene (kindly provided by Gregory Dressler, University of Michigan), described in [ ], was linearized using the ApaL1 restriction enzyme (NEB). .. The linearized PRS4-EGFP construct was transfected into N33 cells using Lipofectamine LTX (Invitrogen) and stably transfected cell clones were selected using Geneticin (G418, Invitrogen) at 500 μg/ml.

    Construct:

    Article Title: The histone demethylase LSD1 regulates inner ear progenitor differentiation through interactions with Pax2 and the NuRD repressor complex
    Article Snippet: To induce differentiation, N33 cells were cultured in equal mixture of Neurobasal (Gibco) and Advance DMEM/F12 medium (Gibco) supplemented with 100 U/ml penicillin-streptomycin, 1% Glutamax (Gibco), 1% N-2 Supplement (Gibco) and 2% B27 Supplement (Gibco) in 5% CO2 /95% air at 39°C. .. To make stable cells, the PRS4-enhanced green fluorescence protein (EGFP) construct containing the neo gene (kindly provided by Gregory Dressler, University of Michigan), described in [ ], was linearized using the ApaL1 restriction enzyme (NEB). .. The linearized PRS4-EGFP construct was transfected into N33 cells using Lipofectamine LTX (Invitrogen) and stably transfected cell clones were selected using Geneticin (G418, Invitrogen) at 500 μg/ml.

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    New England Biolabs apal1 restriction enzyme
    Apal1 Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apal1 restriction enzyme/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apal1 restriction enzyme - by Bioz Stars, 2021-07
    86/100 stars
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