apai  (TaKaRa)

 
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    Name:
    Apa I
    Description:
    G GGCC | CC | CCGG G
    Catalog Number:
    1005b
    Price:
    None
    Size:
    50 000 Units
    Category:
    ApaI Restriction enzymes Cloning
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    Structured Review

    TaKaRa apai
    <t>ApaI</t> PFGE profiles of representative A. <t>baumannii</t> Isolates. (A–J): isolates from Hospital-1 to -10, respectively. The 85% was set as a cutoff to define PFGE types. The dendrogram was generated by the BioNumerics software.
    G GGCC | CC | CCGG G
    https://www.bioz.com/result/apai/product/TaKaRa
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    apai - by Bioz Stars, 2020-05
    94/100 stars

    Images

    1) Product Images from "Molecular Epidemiology of Multi-Drug Resistant Acinetobacter baumannii Isolated in Shandong, China"

    Article Title: Molecular Epidemiology of Multi-Drug Resistant Acinetobacter baumannii Isolated in Shandong, China

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2016.01687

    ApaI PFGE profiles of representative A. baumannii Isolates. (A–J): isolates from Hospital-1 to -10, respectively. The 85% was set as a cutoff to define PFGE types. The dendrogram was generated by the BioNumerics software.
    Figure Legend Snippet: ApaI PFGE profiles of representative A. baumannii Isolates. (A–J): isolates from Hospital-1 to -10, respectively. The 85% was set as a cutoff to define PFGE types. The dendrogram was generated by the BioNumerics software.

    Techniques Used: Generated, Software

    Related Articles

    Clone Assay:

    Article Title: Activity of the HIV-1 Attachment Inhibitor BMS-626529, the Active Component of the Prodrug BMS-663068, against CD4-Independent Viruses and HIV-1 Envelopes Resistant to Other Entry Inhibitors
    Article Snippet: .. For the experiments with the HIV-8x envelope, the gp160 genes of LAI and HIV-8x were cloned into ApaI and NotI sites of the pCMV-HA expression vector (Clontech, Mountain View, CA) by using an In-Fusion HD EcoDry kit (Clontech). .. The mutant envelopes were generated through SDM on the cloned LAI envelope by using a QuikChange II XL site-directed mutagenesis kit (Stratagene, La Jolla, CA).

    Article Title: Localization in the Nucleolus and Coiled Bodies of Protein Subunits of the Ribonucleoprotein Ribonuclease P
    Article Snippet: .. Gene Constructs A PstI-NotI Rpp38 cDNA ( ) fragment subcloned in pBluescript was released by PstI and ApaI (located in the multiple cloning site) and subcloned in-frame in PstI-ApaI digested pEGFP-C1 (CLONTECH Laboratories) to generate pEGFP-Rpp38. pEGFP-Rpp38(246-283) was generated by cleaving pEGFP-Rpp38 with HindIII, deleting the first 245 amino acids of Rpp38, and then the plasmid was self-ligated in the presence of a short HindIII DNA adaptor to keep the carboxy terminal 37 amino acids of Rpp38 (positions 246–283) in-frame with GFP. pEGFP-Rpp38(1-245) was constructed by cleaving a PstI-HindIII Rpp38 cDNA ( ) subcloned in pBluescript with PstI and ApaI and subcloned in-frame in pEGFP-C1 first cleaved with PstI and ApaI. pEGFP-Rpp38(260-283) was generated by subcloning a PstI-ApaI deoxyoligonucleotide that codes for the last 24 amino acids of Rpp38 in pEGFP-C1 digested with PstI and ApaI. pNS38KN was constructed as pEGFP-Rpp38(260-283) with all the nine lysine residues in the carboxy terminal 24–amino acid sequence were substituted with asparagines. pNS38KN23, pNS38KN45, pNS38KN78, and pNS38KN59 were constructed as pEGFP-Rpp38(260-283) but with two lysine substitutions; numbers represent the substituted lysines (see A). .. Constructs with a single substitution of arginine (pNS38R13A), serine (pNS38S18A), threonine (pNS38T22A), or proline (pNS38P23A) to alanine (see positions in A) were also prepared as described for pEGFP-Rpp38(260-283). pNS38ATΔPP was obtained during the construction of pNS38R13A in which we found after sequencing that the arginine and lysine were substituted accidentally by alanine and threonine, respectively, whereas the consecutive proline residues were deleted, keeping the remaining amino acids in-frame with the upstream GFP.

    Article Title: A Model of Superinfection of Virus-Infected Zebrafish Larvae: Increased Susceptibility to Bacteria Associated With Neutrophil Death
    Article Snippet: .. This region was amplified by PCR using pTR339-mCherry2A (Sun et al., 2014) as a template with primers SINV-E1-end-F (GACTAGCACACGAAGA TGA c) and SINvec_Xho-R (AATTCCCCTCGAG GAATTC C), while PTE-3′2 J GFP4-10 was digested by ApaI and XhoI; purified fragments were then reassembled using In-Fusion® HD Cloning Kit Clontech/Takara (#639650), and after transformation in E. coli , plasmid pTE3′2J-3′UTR-339 was obtained. .. The eGFP-2A fragment, and some flanking regions (identical in TE12 and AR339 SINV strains) was then amplified by PCR from pTR339-EGFP2A using primers SINV-C-pml-F (GGTAATGAAACCTCTGcacg) and SIN-E3-stu-R (ATTGAGCAGGGTATCGTagg), and was then subcloned into pTE3′2J-3′UTR339 digested by PmlI and Stu I enzyme.

    Amplification:

    Article Title: A Model of Superinfection of Virus-Infected Zebrafish Larvae: Increased Susceptibility to Bacteria Associated With Neutrophil Death
    Article Snippet: .. This region was amplified by PCR using pTR339-mCherry2A (Sun et al., 2014) as a template with primers SINV-E1-end-F (GACTAGCACACGAAGA TGA c) and SINvec_Xho-R (AATTCCCCTCGAG GAATTC C), while PTE-3′2 J GFP4-10 was digested by ApaI and XhoI; purified fragments were then reassembled using In-Fusion® HD Cloning Kit Clontech/Takara (#639650), and after transformation in E. coli , plasmid pTE3′2J-3′UTR-339 was obtained. .. The eGFP-2A fragment, and some flanking regions (identical in TE12 and AR339 SINV strains) was then amplified by PCR from pTR339-EGFP2A using primers SINV-C-pml-F (GGTAATGAAACCTCTGcacg) and SIN-E3-stu-R (ATTGAGCAGGGTATCGTagg), and was then subcloned into pTE3′2J-3′UTR339 digested by PmlI and Stu I enzyme.

    Subcloning:

    Article Title: Localization in the Nucleolus and Coiled Bodies of Protein Subunits of the Ribonucleoprotein Ribonuclease P
    Article Snippet: .. Gene Constructs A PstI-NotI Rpp38 cDNA ( ) fragment subcloned in pBluescript was released by PstI and ApaI (located in the multiple cloning site) and subcloned in-frame in PstI-ApaI digested pEGFP-C1 (CLONTECH Laboratories) to generate pEGFP-Rpp38. pEGFP-Rpp38(246-283) was generated by cleaving pEGFP-Rpp38 with HindIII, deleting the first 245 amino acids of Rpp38, and then the plasmid was self-ligated in the presence of a short HindIII DNA adaptor to keep the carboxy terminal 37 amino acids of Rpp38 (positions 246–283) in-frame with GFP. pEGFP-Rpp38(1-245) was constructed by cleaving a PstI-HindIII Rpp38 cDNA ( ) subcloned in pBluescript with PstI and ApaI and subcloned in-frame in pEGFP-C1 first cleaved with PstI and ApaI. pEGFP-Rpp38(260-283) was generated by subcloning a PstI-ApaI deoxyoligonucleotide that codes for the last 24 amino acids of Rpp38 in pEGFP-C1 digested with PstI and ApaI. pNS38KN was constructed as pEGFP-Rpp38(260-283) with all the nine lysine residues in the carboxy terminal 24–amino acid sequence were substituted with asparagines. pNS38KN23, pNS38KN45, pNS38KN78, and pNS38KN59 were constructed as pEGFP-Rpp38(260-283) but with two lysine substitutions; numbers represent the substituted lysines (see A). .. Constructs with a single substitution of arginine (pNS38R13A), serine (pNS38S18A), threonine (pNS38T22A), or proline (pNS38P23A) to alanine (see positions in A) were also prepared as described for pEGFP-Rpp38(260-283). pNS38ATΔPP was obtained during the construction of pNS38R13A in which we found after sequencing that the arginine and lysine were substituted accidentally by alanine and threonine, respectively, whereas the consecutive proline residues were deleted, keeping the remaining amino acids in-frame with the upstream GFP.

    Construct:

    Article Title: Localization in the Nucleolus and Coiled Bodies of Protein Subunits of the Ribonucleoprotein Ribonuclease P
    Article Snippet: .. Gene Constructs A PstI-NotI Rpp38 cDNA ( ) fragment subcloned in pBluescript was released by PstI and ApaI (located in the multiple cloning site) and subcloned in-frame in PstI-ApaI digested pEGFP-C1 (CLONTECH Laboratories) to generate pEGFP-Rpp38. pEGFP-Rpp38(246-283) was generated by cleaving pEGFP-Rpp38 with HindIII, deleting the first 245 amino acids of Rpp38, and then the plasmid was self-ligated in the presence of a short HindIII DNA adaptor to keep the carboxy terminal 37 amino acids of Rpp38 (positions 246–283) in-frame with GFP. pEGFP-Rpp38(1-245) was constructed by cleaving a PstI-HindIII Rpp38 cDNA ( ) subcloned in pBluescript with PstI and ApaI and subcloned in-frame in pEGFP-C1 first cleaved with PstI and ApaI. pEGFP-Rpp38(260-283) was generated by subcloning a PstI-ApaI deoxyoligonucleotide that codes for the last 24 amino acids of Rpp38 in pEGFP-C1 digested with PstI and ApaI. pNS38KN was constructed as pEGFP-Rpp38(260-283) with all the nine lysine residues in the carboxy terminal 24–amino acid sequence were substituted with asparagines. pNS38KN23, pNS38KN45, pNS38KN78, and pNS38KN59 were constructed as pEGFP-Rpp38(260-283) but with two lysine substitutions; numbers represent the substituted lysines (see A). .. Constructs with a single substitution of arginine (pNS38R13A), serine (pNS38S18A), threonine (pNS38T22A), or proline (pNS38P23A) to alanine (see positions in A) were also prepared as described for pEGFP-Rpp38(260-283). pNS38ATΔPP was obtained during the construction of pNS38R13A in which we found after sequencing that the arginine and lysine were substituted accidentally by alanine and threonine, respectively, whereas the consecutive proline residues were deleted, keeping the remaining amino acids in-frame with the upstream GFP.

    Purification:

    Article Title: A Model of Superinfection of Virus-Infected Zebrafish Larvae: Increased Susceptibility to Bacteria Associated With Neutrophil Death
    Article Snippet: .. This region was amplified by PCR using pTR339-mCherry2A (Sun et al., 2014) as a template with primers SINV-E1-end-F (GACTAGCACACGAAGA TGA c) and SINvec_Xho-R (AATTCCCCTCGAG GAATTC C), while PTE-3′2 J GFP4-10 was digested by ApaI and XhoI; purified fragments were then reassembled using In-Fusion® HD Cloning Kit Clontech/Takara (#639650), and after transformation in E. coli , plasmid pTE3′2J-3′UTR-339 was obtained. .. The eGFP-2A fragment, and some flanking regions (identical in TE12 and AR339 SINV strains) was then amplified by PCR from pTR339-EGFP2A using primers SINV-C-pml-F (GGTAATGAAACCTCTGcacg) and SIN-E3-stu-R (ATTGAGCAGGGTATCGTagg), and was then subcloned into pTE3′2J-3′UTR339 digested by PmlI and Stu I enzyme.

    Electrophoresis:

    Article Title: Distribution of virulence-associated genes and antimicrobial susceptibility in clinical Acinetobacter baumannii isolates
    Article Snippet: .. After the genomic DNA was digested with ApaI (TaKaRa, Dalian, China), the DNA fragments were separated by electrophoresis in 1% gold agarose (Lonza) in 0.5 × Tris - borate - EDTA buffer with a CHEF apparatus (CHEF Mapper XA, Bio-Rad, USA). .. The conditions included 14°C and 6V/cm with alternating pulses at a 120° angle with a 5-35s pulse time gradient for a total of 22 h. The same method was also used to extract the Salmonella enterica serotype Braenderup H9812 genomic DNAs, which was used as the size marker after being digested by Xba I (TaKaRa, Dalian, China) [ ].

    Sequencing:

    Article Title: Localization in the Nucleolus and Coiled Bodies of Protein Subunits of the Ribonucleoprotein Ribonuclease P
    Article Snippet: .. Gene Constructs A PstI-NotI Rpp38 cDNA ( ) fragment subcloned in pBluescript was released by PstI and ApaI (located in the multiple cloning site) and subcloned in-frame in PstI-ApaI digested pEGFP-C1 (CLONTECH Laboratories) to generate pEGFP-Rpp38. pEGFP-Rpp38(246-283) was generated by cleaving pEGFP-Rpp38 with HindIII, deleting the first 245 amino acids of Rpp38, and then the plasmid was self-ligated in the presence of a short HindIII DNA adaptor to keep the carboxy terminal 37 amino acids of Rpp38 (positions 246–283) in-frame with GFP. pEGFP-Rpp38(1-245) was constructed by cleaving a PstI-HindIII Rpp38 cDNA ( ) subcloned in pBluescript with PstI and ApaI and subcloned in-frame in pEGFP-C1 first cleaved with PstI and ApaI. pEGFP-Rpp38(260-283) was generated by subcloning a PstI-ApaI deoxyoligonucleotide that codes for the last 24 amino acids of Rpp38 in pEGFP-C1 digested with PstI and ApaI. pNS38KN was constructed as pEGFP-Rpp38(260-283) with all the nine lysine residues in the carboxy terminal 24–amino acid sequence were substituted with asparagines. pNS38KN23, pNS38KN45, pNS38KN78, and pNS38KN59 were constructed as pEGFP-Rpp38(260-283) but with two lysine substitutions; numbers represent the substituted lysines (see A). .. Constructs with a single substitution of arginine (pNS38R13A), serine (pNS38S18A), threonine (pNS38T22A), or proline (pNS38P23A) to alanine (see positions in A) were also prepared as described for pEGFP-Rpp38(260-283). pNS38ATΔPP was obtained during the construction of pNS38R13A in which we found after sequencing that the arginine and lysine were substituted accidentally by alanine and threonine, respectively, whereas the consecutive proline residues were deleted, keeping the remaining amino acids in-frame with the upstream GFP.

    Generated:

    Article Title: Localization in the Nucleolus and Coiled Bodies of Protein Subunits of the Ribonucleoprotein Ribonuclease P
    Article Snippet: .. Gene Constructs A PstI-NotI Rpp38 cDNA ( ) fragment subcloned in pBluescript was released by PstI and ApaI (located in the multiple cloning site) and subcloned in-frame in PstI-ApaI digested pEGFP-C1 (CLONTECH Laboratories) to generate pEGFP-Rpp38. pEGFP-Rpp38(246-283) was generated by cleaving pEGFP-Rpp38 with HindIII, deleting the first 245 amino acids of Rpp38, and then the plasmid was self-ligated in the presence of a short HindIII DNA adaptor to keep the carboxy terminal 37 amino acids of Rpp38 (positions 246–283) in-frame with GFP. pEGFP-Rpp38(1-245) was constructed by cleaving a PstI-HindIII Rpp38 cDNA ( ) subcloned in pBluescript with PstI and ApaI and subcloned in-frame in pEGFP-C1 first cleaved with PstI and ApaI. pEGFP-Rpp38(260-283) was generated by subcloning a PstI-ApaI deoxyoligonucleotide that codes for the last 24 amino acids of Rpp38 in pEGFP-C1 digested with PstI and ApaI. pNS38KN was constructed as pEGFP-Rpp38(260-283) with all the nine lysine residues in the carboxy terminal 24–amino acid sequence were substituted with asparagines. pNS38KN23, pNS38KN45, pNS38KN78, and pNS38KN59 were constructed as pEGFP-Rpp38(260-283) but with two lysine substitutions; numbers represent the substituted lysines (see A). .. Constructs with a single substitution of arginine (pNS38R13A), serine (pNS38S18A), threonine (pNS38T22A), or proline (pNS38P23A) to alanine (see positions in A) were also prepared as described for pEGFP-Rpp38(260-283). pNS38ATΔPP was obtained during the construction of pNS38R13A in which we found after sequencing that the arginine and lysine were substituted accidentally by alanine and threonine, respectively, whereas the consecutive proline residues were deleted, keeping the remaining amino acids in-frame with the upstream GFP.

    Expressing:

    Article Title: Activity of the HIV-1 Attachment Inhibitor BMS-626529, the Active Component of the Prodrug BMS-663068, against CD4-Independent Viruses and HIV-1 Envelopes Resistant to Other Entry Inhibitors
    Article Snippet: .. For the experiments with the HIV-8x envelope, the gp160 genes of LAI and HIV-8x were cloned into ApaI and NotI sites of the pCMV-HA expression vector (Clontech, Mountain View, CA) by using an In-Fusion HD EcoDry kit (Clontech). .. The mutant envelopes were generated through SDM on the cloned LAI envelope by using a QuikChange II XL site-directed mutagenesis kit (Stratagene, La Jolla, CA).

    Polymerase Chain Reaction:

    Article Title: A Model of Superinfection of Virus-Infected Zebrafish Larvae: Increased Susceptibility to Bacteria Associated With Neutrophil Death
    Article Snippet: .. This region was amplified by PCR using pTR339-mCherry2A (Sun et al., 2014) as a template with primers SINV-E1-end-F (GACTAGCACACGAAGA TGA c) and SINvec_Xho-R (AATTCCCCTCGAG GAATTC C), while PTE-3′2 J GFP4-10 was digested by ApaI and XhoI; purified fragments were then reassembled using In-Fusion® HD Cloning Kit Clontech/Takara (#639650), and after transformation in E. coli , plasmid pTE3′2J-3′UTR-339 was obtained. .. The eGFP-2A fragment, and some flanking regions (identical in TE12 and AR339 SINV strains) was then amplified by PCR from pTR339-EGFP2A using primers SINV-C-pml-F (GGTAATGAAACCTCTGcacg) and SIN-E3-stu-R (ATTGAGCAGGGTATCGTagg), and was then subcloned into pTE3′2J-3′UTR339 digested by PmlI and Stu I enzyme.

    Article Title: Topogenesis and Homeostasis of Fatty Acyl-CoA Reductase 1 *
    Article Snippet: .. The PCR products were digested with BglII and ApaI and were ligated between the BglII and ApaI sites of pEGFP-C1 (Clontech). .. Because the BglII site was used to construct EGFP-Far1490–515 , the asparagine residue at position 491 of Far1 was changed to serine.

    Transformation Assay:

    Article Title: A Model of Superinfection of Virus-Infected Zebrafish Larvae: Increased Susceptibility to Bacteria Associated With Neutrophil Death
    Article Snippet: .. This region was amplified by PCR using pTR339-mCherry2A (Sun et al., 2014) as a template with primers SINV-E1-end-F (GACTAGCACACGAAGA TGA c) and SINvec_Xho-R (AATTCCCCTCGAG GAATTC C), while PTE-3′2 J GFP4-10 was digested by ApaI and XhoI; purified fragments were then reassembled using In-Fusion® HD Cloning Kit Clontech/Takara (#639650), and after transformation in E. coli , plasmid pTE3′2J-3′UTR-339 was obtained. .. The eGFP-2A fragment, and some flanking regions (identical in TE12 and AR339 SINV strains) was then amplified by PCR from pTR339-EGFP2A using primers SINV-C-pml-F (GGTAATGAAACCTCTGcacg) and SIN-E3-stu-R (ATTGAGCAGGGTATCGTagg), and was then subcloned into pTE3′2J-3′UTR339 digested by PmlI and Stu I enzyme.

    Plasmid Preparation:

    Article Title: Activity of the HIV-1 Attachment Inhibitor BMS-626529, the Active Component of the Prodrug BMS-663068, against CD4-Independent Viruses and HIV-1 Envelopes Resistant to Other Entry Inhibitors
    Article Snippet: .. For the experiments with the HIV-8x envelope, the gp160 genes of LAI and HIV-8x were cloned into ApaI and NotI sites of the pCMV-HA expression vector (Clontech, Mountain View, CA) by using an In-Fusion HD EcoDry kit (Clontech). .. The mutant envelopes were generated through SDM on the cloned LAI envelope by using a QuikChange II XL site-directed mutagenesis kit (Stratagene, La Jolla, CA).

    Article Title: Localization in the Nucleolus and Coiled Bodies of Protein Subunits of the Ribonucleoprotein Ribonuclease P
    Article Snippet: .. Gene Constructs A PstI-NotI Rpp38 cDNA ( ) fragment subcloned in pBluescript was released by PstI and ApaI (located in the multiple cloning site) and subcloned in-frame in PstI-ApaI digested pEGFP-C1 (CLONTECH Laboratories) to generate pEGFP-Rpp38. pEGFP-Rpp38(246-283) was generated by cleaving pEGFP-Rpp38 with HindIII, deleting the first 245 amino acids of Rpp38, and then the plasmid was self-ligated in the presence of a short HindIII DNA adaptor to keep the carboxy terminal 37 amino acids of Rpp38 (positions 246–283) in-frame with GFP. pEGFP-Rpp38(1-245) was constructed by cleaving a PstI-HindIII Rpp38 cDNA ( ) subcloned in pBluescript with PstI and ApaI and subcloned in-frame in pEGFP-C1 first cleaved with PstI and ApaI. pEGFP-Rpp38(260-283) was generated by subcloning a PstI-ApaI deoxyoligonucleotide that codes for the last 24 amino acids of Rpp38 in pEGFP-C1 digested with PstI and ApaI. pNS38KN was constructed as pEGFP-Rpp38(260-283) with all the nine lysine residues in the carboxy terminal 24–amino acid sequence were substituted with asparagines. pNS38KN23, pNS38KN45, pNS38KN78, and pNS38KN59 were constructed as pEGFP-Rpp38(260-283) but with two lysine substitutions; numbers represent the substituted lysines (see A). .. Constructs with a single substitution of arginine (pNS38R13A), serine (pNS38S18A), threonine (pNS38T22A), or proline (pNS38P23A) to alanine (see positions in A) were also prepared as described for pEGFP-Rpp38(260-283). pNS38ATΔPP was obtained during the construction of pNS38R13A in which we found after sequencing that the arginine and lysine were substituted accidentally by alanine and threonine, respectively, whereas the consecutive proline residues were deleted, keeping the remaining amino acids in-frame with the upstream GFP.

    Article Title: A Model of Superinfection of Virus-Infected Zebrafish Larvae: Increased Susceptibility to Bacteria Associated With Neutrophil Death
    Article Snippet: .. This region was amplified by PCR using pTR339-mCherry2A (Sun et al., 2014) as a template with primers SINV-E1-end-F (GACTAGCACACGAAGA TGA c) and SINvec_Xho-R (AATTCCCCTCGAG GAATTC C), while PTE-3′2 J GFP4-10 was digested by ApaI and XhoI; purified fragments were then reassembled using In-Fusion® HD Cloning Kit Clontech/Takara (#639650), and after transformation in E. coli , plasmid pTE3′2J-3′UTR-339 was obtained. .. The eGFP-2A fragment, and some flanking regions (identical in TE12 and AR339 SINV strains) was then amplified by PCR from pTR339-EGFP2A using primers SINV-C-pml-F (GGTAATGAAACCTCTGcacg) and SIN-E3-stu-R (ATTGAGCAGGGTATCGTagg), and was then subcloned into pTE3′2J-3′UTR339 digested by PmlI and Stu I enzyme.

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  • apai  (TaKaRa)
    94
    TaKaRa apai
    <t>ApaI</t> PFGE profiles of representative A. <t>baumannii</t> Isolates. (A–J): isolates from Hospital-1 to -10, respectively. The 85% was set as a cutoff to define PFGE types. The dendrogram was generated by the BioNumerics software.
    Apai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apai/product/TaKaRa
    Average 94 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    apai - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    85
    TaKaRa kpni apai fragment
    <t>ApaI</t> PFGE profiles of representative A. <t>baumannii</t> Isolates. (A–J): isolates from Hospital-1 to -10, respectively. The 85% was set as a cutoff to define PFGE types. The dendrogram was generated by the BioNumerics software.
    Kpni Apai Fragment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kpni apai fragment/product/TaKaRa
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    kpni apai fragment - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    Image Search Results


    ApaI PFGE profiles of representative A. baumannii Isolates. (A–J): isolates from Hospital-1 to -10, respectively. The 85% was set as a cutoff to define PFGE types. The dendrogram was generated by the BioNumerics software.

    Journal: Frontiers in Microbiology

    Article Title: Molecular Epidemiology of Multi-Drug Resistant Acinetobacter baumannii Isolated in Shandong, China

    doi: 10.3389/fmicb.2016.01687

    Figure Lengend Snippet: ApaI PFGE profiles of representative A. baumannii Isolates. (A–J): isolates from Hospital-1 to -10, respectively. The 85% was set as a cutoff to define PFGE types. The dendrogram was generated by the BioNumerics software.

    Article Snippet: In brief, the chromosomal DNA of A. baumannii was digested with 60 U of ApaI (Takara, Dalian, China) in a 37°C water bath.

    Techniques: Generated, Software