antirabbit igg  (Millipore)


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  • 95
    Name:
    Anti Rabbit IgG antibody
    Description:

    Catalog Number:
    gw20085f
    Price:
    None
    Applications:
    Anti-Rabbit IgG antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.
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    Structured Review

    Millipore antirabbit igg

    https://www.bioz.com/result/antirabbit igg/product/Millipore
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    antirabbit igg - by Bioz Stars, 2020-08
    95/100 stars

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    Related Articles

    Centrifugation:

    Article Title: The N-terminal domain of the thermo-regulated surface protein PrpA of Enterococcus faecium binds to fibrinogen, fibronectin and platelets
    Article Snippet: .. Samples of the cultures were taken during different time points of the growth curve (A660 = 0.3, 0.5, 0.7, 1.0) and pelleted by centrifugation at 6,500 g for 1 min. PrpA was detected using 1:100 anti-PrpA rabbit IgG and 1:50 FITC-labeled goat anti rabbit IgG (Sigma-Aldrich). .. Flow cytometry was performed using the FACS Calibur system (BD Biosciences, Breda, The Netherlands).

    Positive Control:

    Article Title: Structural and Functional Characterization of SporoSAG
    Article Snippet: .. Positive reactions were detected using horseradish peroxidase-conjugated anti-human, anti-mouse, or anti-rabbit polyvalent IgG, IgA, and IgM detection antibodies (Sigma), developed with 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) peroxidase substrate (Kirkegaard & Perry Laboratories, Inc.), and the absorbance was measured at 405 nm after > 60 min. Serum samples from a known uninfected volunteer served as a negative control, and serum from a patient infected with Type II strain oocysts was included as the positive control, respectively. .. For murine and rabbit infections, positive control sera were derived by injecting bradyzoite cysts or sporulated oocysts of the Type II Me49 strain via the oral (bradyzoites, oocysts) and subcutaneous routes (oocysts).

    Negative Control:

    Article Title: Structural and Functional Characterization of SporoSAG
    Article Snippet: .. Positive reactions were detected using horseradish peroxidase-conjugated anti-human, anti-mouse, or anti-rabbit polyvalent IgG, IgA, and IgM detection antibodies (Sigma), developed with 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) peroxidase substrate (Kirkegaard & Perry Laboratories, Inc.), and the absorbance was measured at 405 nm after > 60 min. Serum samples from a known uninfected volunteer served as a negative control, and serum from a patient infected with Type II strain oocysts was included as the positive control, respectively. .. For murine and rabbit infections, positive control sera were derived by injecting bradyzoite cysts or sporulated oocysts of the Type II Me49 strain via the oral (bradyzoites, oocysts) and subcutaneous routes (oocysts).

    Infection:

    Article Title: Structural and Functional Characterization of SporoSAG
    Article Snippet: .. Positive reactions were detected using horseradish peroxidase-conjugated anti-human, anti-mouse, or anti-rabbit polyvalent IgG, IgA, and IgM detection antibodies (Sigma), developed with 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) peroxidase substrate (Kirkegaard & Perry Laboratories, Inc.), and the absorbance was measured at 405 nm after > 60 min. Serum samples from a known uninfected volunteer served as a negative control, and serum from a patient infected with Type II strain oocysts was included as the positive control, respectively. .. For murine and rabbit infections, positive control sera were derived by injecting bradyzoite cysts or sporulated oocysts of the Type II Me49 strain via the oral (bradyzoites, oocysts) and subcutaneous routes (oocysts).

    Immunoprecipitation:

    Article Title: Insights into Role of Bromodomain, Testis-specific (Brdt) in Acetylated Histone H4-dependent Chromatin Remodeling in Mammalian Spermiogenesis *
    Article Snippet: .. Immunoprecipitation was performed in parallel with 10 μg of anti-Brdt antibody (mouse) and mock rabbit IgG antibody (Sigma) for 4 h. Protein A-agarose beads after pulldown were washed twice in nuclear lysis buffer and twice in 1× PBST (0.1% Tween 20). .. Samples were loaded on a 12% SDS-PAGE after boiling directly in 5× SDS sample buffer.

    Article Title: Histone Deacetylase Inhibitor Sensitizes ERCC1-High Non-small-Cell Lung Cancer Cells to Cisplatin via Regulating miR-149
    Article Snippet: .. The chromatin was immunoprecipitated by either anti-immunoglobulin G (IgG) or anti-E2F1 antibody. ..

    Article Title: Drosophila myeloid leukemia factor acts with DREF to activate the JNK signaling pathway
    Article Snippet: .. Immunoprecipitation was performed with 800 μg of lysates with anti-dMLF rabbit IgG or the normal rabbit IgG (Sigma-Aldrich) and protein A–Sepharose (GE Healthcare). ..

    Incubation:

    Article Title: Structure, Expression, and Functional Analysis of the Gene Coding for Calmodulin in the Chytridiomycete Blastocladiella emersonii
    Article Snippet: .. The protein blot was then washed with TBS (10 mM Tris-HCl [pH 7.5], 150 mM NaCl) containing 0.05% Tween 20 followed by TBS alone and was incubated with anti-rabbit immunoglobulin G antiserum conjugated with alkaline phosphatase (Sigma). ..

    other:

    Article Title: Can antibodies with specificity for soluble antigens mimic the therapeutic effects of intravenous IgG in the treatment of autoimmune disease?
    Article Snippet: OVA (grade V), rabbit IgG anti-OVA, 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide (EDAC), and PKH26 Red Fluorescent Cell Linker Kit were purchased from Sigma-Aldrich.

    Lysis:

    Article Title: Insights into Role of Bromodomain, Testis-specific (Brdt) in Acetylated Histone H4-dependent Chromatin Remodeling in Mammalian Spermiogenesis *
    Article Snippet: .. Immunoprecipitation was performed in parallel with 10 μg of anti-Brdt antibody (mouse) and mock rabbit IgG antibody (Sigma) for 4 h. Protein A-agarose beads after pulldown were washed twice in nuclear lysis buffer and twice in 1× PBST (0.1% Tween 20). .. Samples were loaded on a 12% SDS-PAGE after boiling directly in 5× SDS sample buffer.

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