antirabbit igg hrp  (Millipore)


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    Name:
    HRP conjugated Anti Rabbit IgG Concentrate
    Description:
    Immunoglobulin G IgG belongs to the immunoglobulin family and is a widely expressed serum antibody It consists of a gamma γ heavy chain in the constant C region The monomeric 150 kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa respectively The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains linking the heavy and light chains and resides inside the chains IgG is further subdivided into four classes namely IgG1 IgG2 IgG3 and IgG4 with different heavy chains named γ1 γ2 γ3 and γ4 respectively
    Catalog Number:
    rabhrp1
    Price:
    None
    Applications:
    HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1) has been used in Western Blotting and Immunohistochemistry.
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    Structured Review

    Millipore antirabbit igg hrp
    Immunoglobulin G IgG belongs to the immunoglobulin family and is a widely expressed serum antibody It consists of a gamma γ heavy chain in the constant C region The monomeric 150 kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa respectively The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains linking the heavy and light chains and resides inside the chains IgG is further subdivided into four classes namely IgG1 IgG2 IgG3 and IgG4 with different heavy chains named γ1 γ2 γ3 and γ4 respectively
    https://www.bioz.com/result/antirabbit igg hrp/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    antirabbit igg hrp - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Serological Method Using Recombinant S2 Protein To Differentiate Equine Infectious Anemia Virus (EIAV)-Infected and EIAV-Vaccinated Horses
    Article Snippet: .. In the analyses with rabbit anti-S2 serum as primary antibody in the ELISA, the rabbit immune serum was 1:200 diluted in blocking buffer, and anti-rabbit IgG HRP-conjugated antibody diluted 1:10,000 (Sigma) was used as the secondary antibody. .. For the affinity ELISA procedure (NN-ELISA), HS2G protein was diluted in PBS-bovine serum albumin (BSA; 0.2%) buffer to a final concentration of 10 μg/ml.

    Article Title: The ESAT-6 Protein of Mycobacterium tuberculosis Interacts with Beta-2-Microglobulin (β2M) Affecting Antigen Presentation Function of Macrophage
    Article Snippet: .. A similar sandwich ELISA was used for detection of β2M in the pleural fluid samples where the ELISA plate was coated with 1 µg/well mouse anti-human β2M Ab, clone CBL306 (Millipore, USA) and post blocking pleural fluid samples (50 µl) were added and the captured β2M was detected with the help of rabbit-anti-human β2M Ab (Abcam Inc, USA), diluted 1∶1000 and HRP conjugated anti-rabbit Ab (Sigma-Aldrich, USA), diluted 1∶1000. .. All Ab dilutions were made in blocking buffer (1× PBS+2% BSA).

    Purification:

    Article Title: Porphyromonas gingivalis HmuY and Streptococcus gordonii GAPDH—Novel Heme Acquisition Strategy in the Oral Microbiome
    Article Snippet: .. The protein was probed with rabbit polyclonal anti-SgGAPDH antibodies, raised in this study against purified SgGAPDH protein (ProteoGenix, Schiltigheim, France), and complexes formed detected using HRP-conjugated anti-rabbit IgG antibodies (Sigma) and a substrate solution as described previously [ ]. .. To confirm the presence of SgGAPDH, Western blotting was employed.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Serological Method Using Recombinant S2 Protein To Differentiate Equine Infectious Anemia Virus (EIAV)-Infected and EIAV-Vaccinated Horses
    Article Snippet: .. In the analyses with rabbit anti-S2 serum as primary antibody in the ELISA, the rabbit immune serum was 1:200 diluted in blocking buffer, and anti-rabbit IgG HRP-conjugated antibody diluted 1:10,000 (Sigma) was used as the secondary antibody. .. For the affinity ELISA procedure (NN-ELISA), HS2G protein was diluted in PBS-bovine serum albumin (BSA; 0.2%) buffer to a final concentration of 10 μg/ml.

    Article Title: The ESAT-6 Protein of Mycobacterium tuberculosis Interacts with Beta-2-Microglobulin (β2M) Affecting Antigen Presentation Function of Macrophage
    Article Snippet: .. A similar sandwich ELISA was used for detection of β2M in the pleural fluid samples where the ELISA plate was coated with 1 µg/well mouse anti-human β2M Ab, clone CBL306 (Millipore, USA) and post blocking pleural fluid samples (50 µl) were added and the captured β2M was detected with the help of rabbit-anti-human β2M Ab (Abcam Inc, USA), diluted 1∶1000 and HRP conjugated anti-rabbit Ab (Sigma-Aldrich, USA), diluted 1∶1000. .. All Ab dilutions were made in blocking buffer (1× PBS+2% BSA).

    Sandwich ELISA:

    Article Title: The ESAT-6 Protein of Mycobacterium tuberculosis Interacts with Beta-2-Microglobulin (β2M) Affecting Antigen Presentation Function of Macrophage
    Article Snippet: .. A similar sandwich ELISA was used for detection of β2M in the pleural fluid samples where the ELISA plate was coated with 1 µg/well mouse anti-human β2M Ab, clone CBL306 (Millipore, USA) and post blocking pleural fluid samples (50 µl) were added and the captured β2M was detected with the help of rabbit-anti-human β2M Ab (Abcam Inc, USA), diluted 1∶1000 and HRP conjugated anti-rabbit Ab (Sigma-Aldrich, USA), diluted 1∶1000. .. All Ab dilutions were made in blocking buffer (1× PBS+2% BSA).

    Incubation:

    Article Title: Recombinant Dirofilaria immitis Polyprotein That Stimulates Murine B Cells To Produce Nonspecific Polyclonal Immunoglobulin E Antibody
    Article Snippet: .. Co., Osaka, Japan) at room temperature for 30 min and incubated with mouse (on day 21 after implantation) or rabbit (diluted 1:100) sera at 4°C for 16 h. After washing, the blots were incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgE (diluted 1:500; GAM/IgE [Fc] PO; Nordic Immunological Laboratories B.V., Tilburg, The Netherlands), anti-mouse IgG (diluted 1:500; A90-131P, Bethyl Laboratories, Inc., Montgomery, Tex.), or HRP-conjugated anti-rabbit IgG (diluted 1:1,000, Sigma) at room temperature for 2 h. Bands were revealed by the POD Immunostain Set (Wako). .. B cells were freshly prepared from spleen and peritoneal cavity samples of naive or osmotic pump-implanted mice (on days 7 and 21 after implantation).

    Expressing:

    Article Title: Differential expression of Homeobox C11 protein in water buffalo Bubalus bubalis and its putative 3D structure
    Article Snippet: .. The expression of HOXC11 protein across the tissues of buffalo was determined employing western blot analysis as mentioned above using anti-HOXC11 rabbit sera as primary antibody and HRP conjugated anti-rabbit IgG (Sigma Aldrich, USA) as secondary antibody. β-actin was used as loading control. .. All images were analyzed using Image-J (version 1.44) software.

    Western Blot:

    Article Title: Modulatory Effect of Gliadin Peptide 10-mer on Epithelial Intestinal CACO-2 Cell Inflammatory Response
    Article Snippet: .. Bound antibodies were visualized with HRP-conjugated anti-rabbit IgG or anti-mouse IgG (Sigma) and immunoreactivity was assessed by the chemiluminescence reaction using the ECL Western blot system (Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, UK). .. As a control for nonspecific reactivity, parallel SDS-PAGE gels were blotted as described, using an anti-rabbit IgG or anti-mouse IgG (Sigma).

    Article Title: Differential expression of Homeobox C11 protein in water buffalo Bubalus bubalis and its putative 3D structure
    Article Snippet: .. The expression of HOXC11 protein across the tissues of buffalo was determined employing western blot analysis as mentioned above using anti-HOXC11 rabbit sera as primary antibody and HRP conjugated anti-rabbit IgG (Sigma Aldrich, USA) as secondary antibody. β-actin was used as loading control. .. All images were analyzed using Image-J (version 1.44) software.

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