antigoat igg  (Vector Laboratories)


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    Name:
    Unconjugated Rabbit Anti Goat IgG Antibody
    Description:
    Unconjugated Rabbit Anti Goat IgG H L Antibody is supplied in solution This ultrapure high affinity antibody has been thoroughly adsorbed against serum and immunoglobulins from potentially interfering species and is ready for iodination fluorochrome labeling or enzyme conjugations It can also be employed as a capture antibody in enzyme immunoassays or in other assays requiring carrier free immunoglobulins As well as recognising goat IgG this antibody is suitable to detect sheep and bovine IgG primary antibodies The use of bovine products such as casein serum albumin or non fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross reactivity of this antibody with bovine immunoglobulins that may be present If this antibody is to be used in these applications blocking with Animal Free Blocker solution Cat No SP 5030 is recommended
    Catalog Number:
    ai-5000
    Price:
    None
    Host:
    Rabbit
    Size:
    1 5 mg
    Category:
    Antibodies
    Reactivity:
    Goat
    Buy from Supplier


    Structured Review

    Vector Laboratories antigoat igg
    Unconjugated Rabbit Anti Goat IgG Antibody
    Unconjugated Rabbit Anti Goat IgG H L Antibody is supplied in solution This ultrapure high affinity antibody has been thoroughly adsorbed against serum and immunoglobulins from potentially interfering species and is ready for iodination fluorochrome labeling or enzyme conjugations It can also be employed as a capture antibody in enzyme immunoassays or in other assays requiring carrier free immunoglobulins As well as recognising goat IgG this antibody is suitable to detect sheep and bovine IgG primary antibodies The use of bovine products such as casein serum albumin or non fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross reactivity of this antibody with bovine immunoglobulins that may be present If this antibody is to be used in these applications blocking with Animal Free Blocker solution Cat No SP 5030 is recommended
    https://www.bioz.com/result/antigoat igg/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antigoat igg - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Complement Receptor 2 is Expressed in Neural Progenitor Cells and Regulates Adult Hippocampal Neurogenesis
    Article Snippet: .. For 3,3′-Diaminobenzidine (DAB) staining, sections were incubated with primary antibodies rat anti-BrdU (0.4 µg/ml; Abcam) or goat anti-Dcx (0.4 µg/ml; Santa Cruz Biotechnology) overnight at 4°C, rinsed, and incubated with biotinylated rabbit anti-rat (1 µg/ml, vector) or rabbit anti-goat antibodies (3 µg/ml, (Vector Laboratories) followed by ABC labeling (Vector Laboratories) and development with DAB (Sigma). .. For BrdU labeling, brain sections were pre-treated with 2N HCl at 37°C for 30 min before incubation with primary antibody.

    Staining:

    Article Title: Complement Receptor 2 is Expressed in Neural Progenitor Cells and Regulates Adult Hippocampal Neurogenesis
    Article Snippet: .. For 3,3′-Diaminobenzidine (DAB) staining, sections were incubated with primary antibodies rat anti-BrdU (0.4 µg/ml; Abcam) or goat anti-Dcx (0.4 µg/ml; Santa Cruz Biotechnology) overnight at 4°C, rinsed, and incubated with biotinylated rabbit anti-rat (1 µg/ml, vector) or rabbit anti-goat antibodies (3 µg/ml, (Vector Laboratories) followed by ABC labeling (Vector Laboratories) and development with DAB (Sigma). .. For BrdU labeling, brain sections were pre-treated with 2N HCl at 37°C for 30 min before incubation with primary antibody.

    Incubation:

    Article Title: Extending the duration of hypothermia does not further improve white matter protection after ischemia in term-equivalent fetal sheep
    Article Snippet: .. Sections were incubated for 3 h in biotin-conjugated 1:200 anti-mouse (Olig2, MBP, Vector Laboratories, Burlingame, CA, USA) or 1:200 anti-rabbit (GFAP, Vector Laboratories) 1:200 anti-goat (Iba1, Vector Laboratories) in 3% NGS for Olig2, GFAP and MBP and 3% NHS for Iba1. .. Slides were then incubated in ExtrAvidin® (1:200, Sigma-Aldrich Pty.

    Article Title: Complement Receptor 2 is Expressed in Neural Progenitor Cells and Regulates Adult Hippocampal Neurogenesis
    Article Snippet: .. For 3,3′-Diaminobenzidine (DAB) staining, sections were incubated with primary antibodies rat anti-BrdU (0.4 µg/ml; Abcam) or goat anti-Dcx (0.4 µg/ml; Santa Cruz Biotechnology) overnight at 4°C, rinsed, and incubated with biotinylated rabbit anti-rat (1 µg/ml, vector) or rabbit anti-goat antibodies (3 µg/ml, (Vector Laboratories) followed by ABC labeling (Vector Laboratories) and development with DAB (Sigma). .. For BrdU labeling, brain sections were pre-treated with 2N HCl at 37°C for 30 min before incubation with primary antibody.

    Article Title: Oxytocin has sex-specific effects on social behaviour and hypothalamic oxytocin immunoreactive cells but not hippocampal neurogenesis in adult rats
    Article Snippet: .. Tissue was rinsed five times for ten minutes in PBS and then incubated overnight at 4°C in a rabbit anti-goat secondary (1:500; Vector Laboratories) diluted in PBS. .. Sections were then washed five times for ten minutes in PBS and then incubated in an avidin-biotin horseradish peroxidase complex (1:1000; ABC Vector Elite kit; Vector Laboratories) for 4 h at room temperature.

    Article Title: Expression in CHO Cells and Pharmacokinetics and Brain Uptake in the Rhesus Monkey of an IgG-Iduronate-2-Sulfatase Fusion Protein
    Article Snippet: .. Following washing with TBST, a conjugate of rabbit anti-goat IgG and alkaline phosphatase (#AP-1000, Vector Labs, Burlingame, CA) was applied in a volume of 100 uL (500 ng)/well followed by a 45 min incubation at RT. .. The wells were washed with TBST, and 100 uL/well of p-nitrophenylphosphate (#P5994, Sigma Chemical Co., St. Louis, MO) was incubated in the dark at RT for 30 min.

    Article Title: EphB signaling regulates target innervation in the developing and deafferented auditory brainstem
    Article Snippet: .. The following day, slides were rinsed in TBS, incubated with a biotinylated secondary antibody, either goat anti-rabbit, goat anti-mouse, or rabbit anti-goat (6 µg/ml, Vector Laboratories), in 1% serum in TBST for 2 hours, rinsed with TBS, and then incubated in ABC solution (Vector Laboratories) for 1 hour. .. Slides were then rinsed with TBS and reacted with 3,3’-diaminobenzidine, rinsed, dehydrated in a graded series of ethanol to xylenes, and coverslipped with DPX mountant (VWR International).

    Article Title: Immunophenotypic characterization of telocyte-like cells in pterygium
    Article Snippet: .. Biotinylated anti-mouse, anti-rabbit, or anti-goat was used as secondary antisera (1:200; Vector Laboratories, Burlingame, CA) and incubated for 30 min at RT. .. The sections were further incubated in alkaline phosphatase streptavidin (1:1,000, Vector Laboratories) for 30 min at RT and reacted with the SIGMAFASTTM Fast red substrate–chromogen system (Sigma, St. Louis, MO).

    Next-Generation Sequencing:

    Article Title: Extending the duration of hypothermia does not further improve white matter protection after ischemia in term-equivalent fetal sheep
    Article Snippet: .. Sections were incubated for 3 h in biotin-conjugated 1:200 anti-mouse (Olig2, MBP, Vector Laboratories, Burlingame, CA, USA) or 1:200 anti-rabbit (GFAP, Vector Laboratories) 1:200 anti-goat (Iba1, Vector Laboratories) in 3% NGS for Olig2, GFAP and MBP and 3% NHS for Iba1. .. Slides were then incubated in ExtrAvidin® (1:200, Sigma-Aldrich Pty.

    Labeling:

    Article Title: Complement Receptor 2 is Expressed in Neural Progenitor Cells and Regulates Adult Hippocampal Neurogenesis
    Article Snippet: .. For 3,3′-Diaminobenzidine (DAB) staining, sections were incubated with primary antibodies rat anti-BrdU (0.4 µg/ml; Abcam) or goat anti-Dcx (0.4 µg/ml; Santa Cruz Biotechnology) overnight at 4°C, rinsed, and incubated with biotinylated rabbit anti-rat (1 µg/ml, vector) or rabbit anti-goat antibodies (3 µg/ml, (Vector Laboratories) followed by ABC labeling (Vector Laboratories) and development with DAB (Sigma). .. For BrdU labeling, brain sections were pre-treated with 2N HCl at 37°C for 30 min before incubation with primary antibody.

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