anti huc hud  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88

    Structured Review

    Thermo Fisher anti huc hud
    Characterization of neuronal differentiation of TSC2 +/+ and TSC2 −/− cells in 25 mM and 5 mM glucose media. (A) Positivity for the proliferation marker Ki67 and the cell fate markers GFAP, <t>Nestin,</t> TUJ1, and <t>HuC/HuD</t> was measured using flow cytometry during cortical neuronal differentiation at days 10, 18 (Ki67 at day 35 in TSC2 −/− cultures in 5 mM glucose), 50, and 80. In 25 mM glucose media, TSC2 +/+ and TSC2 −/− cells expressed differentiation markers similarly. Cell cycle activity increased between the neural progenitor stage at day 18 and day 50 after which most TSC2 +/+ cells entered a cell cycle inactive state. Ongoing proliferation beyond day 50 could be observed in TSC2 −/− cultures. Lowering the glucose level changed the expression profile of the differentiation markers in TSC2 −/− cultures, whereas the differentiation of TSC2 +/+ cells was not affected. TSC2 −/− cells in low glucose media lacked the decline in Nestin expression and were characterized by an increase in GFAP expression from day 50 onwards. The proportion of HuC/HuD-positive cells declined from 19.5% at day 50 to 2.7% at day 80. In contrast to the 25 mM glucose condition, cell cycle activity could be detected in 75% of TSC2 +/+ cells at day 80, whereas TSC2 −/− completely lost their cell cycle activity after day 50 (n = 1 for each differentiation). (B) Example flow cytometry plots for GFAP and HuC/HuD for TSC2 +/+ and TSC2 −/− cultures in 25 mM and 5 mM glucose media at respective timepoints showing no significant difference between cell lines throughout the whole differentiation regardless of media, except for TSC2 −/− cells in 5 mM glucose media at day 80 with an increase in the GFAP-positive and a decrease in the HuC/HuD-positive population.
    Anti Huc Hud, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 5684 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti huc hud/product/Thermo Fisher
    Average 88 stars, based on 5684 article reviews
    Price from $9.99 to $1999.99
    anti huc hud - by Bioz Stars, 2020-09
    88/100 stars

    Images

    1) Product Images from "High glucose concentrations mask cellular phenotypes in a stem cell model of tuberous sclerosis complex"

    Article Title: High glucose concentrations mask cellular phenotypes in a stem cell model of tuberous sclerosis complex

    Journal: Epilepsy & Behavior

    doi: 10.1016/j.yebeh.2019.106581

    Characterization of neuronal differentiation of TSC2 +/+ and TSC2 −/− cells in 25 mM and 5 mM glucose media. (A) Positivity for the proliferation marker Ki67 and the cell fate markers GFAP, Nestin, TUJ1, and HuC/HuD was measured using flow cytometry during cortical neuronal differentiation at days 10, 18 (Ki67 at day 35 in TSC2 −/− cultures in 5 mM glucose), 50, and 80. In 25 mM glucose media, TSC2 +/+ and TSC2 −/− cells expressed differentiation markers similarly. Cell cycle activity increased between the neural progenitor stage at day 18 and day 50 after which most TSC2 +/+ cells entered a cell cycle inactive state. Ongoing proliferation beyond day 50 could be observed in TSC2 −/− cultures. Lowering the glucose level changed the expression profile of the differentiation markers in TSC2 −/− cultures, whereas the differentiation of TSC2 +/+ cells was not affected. TSC2 −/− cells in low glucose media lacked the decline in Nestin expression and were characterized by an increase in GFAP expression from day 50 onwards. The proportion of HuC/HuD-positive cells declined from 19.5% at day 50 to 2.7% at day 80. In contrast to the 25 mM glucose condition, cell cycle activity could be detected in 75% of TSC2 +/+ cells at day 80, whereas TSC2 −/− completely lost their cell cycle activity after day 50 (n = 1 for each differentiation). (B) Example flow cytometry plots for GFAP and HuC/HuD for TSC2 +/+ and TSC2 −/− cultures in 25 mM and 5 mM glucose media at respective timepoints showing no significant difference between cell lines throughout the whole differentiation regardless of media, except for TSC2 −/− cells in 5 mM glucose media at day 80 with an increase in the GFAP-positive and a decrease in the HuC/HuD-positive population.
    Figure Legend Snippet: Characterization of neuronal differentiation of TSC2 +/+ and TSC2 −/− cells in 25 mM and 5 mM glucose media. (A) Positivity for the proliferation marker Ki67 and the cell fate markers GFAP, Nestin, TUJ1, and HuC/HuD was measured using flow cytometry during cortical neuronal differentiation at days 10, 18 (Ki67 at day 35 in TSC2 −/− cultures in 5 mM glucose), 50, and 80. In 25 mM glucose media, TSC2 +/+ and TSC2 −/− cells expressed differentiation markers similarly. Cell cycle activity increased between the neural progenitor stage at day 18 and day 50 after which most TSC2 +/+ cells entered a cell cycle inactive state. Ongoing proliferation beyond day 50 could be observed in TSC2 −/− cultures. Lowering the glucose level changed the expression profile of the differentiation markers in TSC2 −/− cultures, whereas the differentiation of TSC2 +/+ cells was not affected. TSC2 −/− cells in low glucose media lacked the decline in Nestin expression and were characterized by an increase in GFAP expression from day 50 onwards. The proportion of HuC/HuD-positive cells declined from 19.5% at day 50 to 2.7% at day 80. In contrast to the 25 mM glucose condition, cell cycle activity could be detected in 75% of TSC2 +/+ cells at day 80, whereas TSC2 −/− completely lost their cell cycle activity after day 50 (n = 1 for each differentiation). (B) Example flow cytometry plots for GFAP and HuC/HuD for TSC2 +/+ and TSC2 −/− cultures in 25 mM and 5 mM glucose media at respective timepoints showing no significant difference between cell lines throughout the whole differentiation regardless of media, except for TSC2 −/− cells in 5 mM glucose media at day 80 with an increase in the GFAP-positive and a decrease in the HuC/HuD-positive population.

    Techniques Used: Marker, Flow Cytometry, Cytometry, Activity Assay, Expressing

    2) Product Images from "Deletion of both the Tyrosine-Based Endocytosis Signal and the Endoplasmic Reticulum Retrieval Signal in the Cytoplasmic Tail of Spike Protein Attenuates Porcine Epidemic Diarrhea Virus in Pigs"

    Article Title: Deletion of both the Tyrosine-Based Endocytosis Signal and the Endoplasmic Reticulum Retrieval Signal in the Cytoplasmic Tail of Spike Protein Attenuates Porcine Epidemic Diarrhea Virus in Pigs

    Journal: Journal of Virology

    doi: 10.1128/JVI.01758-18

    Mutation of the YxxΦ and KVHVQ motifs of PEDV S protein altered the expression of S proteins on the cell surface and syncytium formation. (A) IF staining of S proteins expressed on cell surface, or total (surface and intracellular) S proteins (Total S). Vero cells were fixed by 4% formaldehyde at 12 h after transfection with plasmid DNA. Without permeabilization, surface S proteins were stained with guinea pig antiserum GP17 against PEDV S1 followed by Alexa Fluor 488 (AF488)-conjugated goat anti-guinea pig IgG. Total S proteins were observed under the mCherry channel. Scale bar: 30 μm. (B) The fluorescent intensities of the total S proteins (mCherry) were measured on 50 individual cells of each sample. NS, no statistically significant difference ( P > 0.05). (C) Surface S/total S fluorescent intensity ratios (AF488/mCherry) were calculated based on 50 individual cells of each sample. (D) Syncytia induced by mCherry-tagged WT S proteins and their mutants. At 6 h after transfection with the plasmid DNA, Vero cells were cultured in medium containing trypsin (10 μg/ml) for an additional 6 h. Cells were fixed with methanol and total S proteins were detected by IF staining with the antiserum GP17 as described above. Nuclei were stained with DAPI. Scale bar: 50 μm. (E) Nuclei in each syncytium were counted based on 200 syncytia for each sample. Values in panels B, C, and E are shown in box-whisker plots. The boxes indicate interquartile ranges in different groups, the lines in the boxes represent median values, and the whiskers show the range of a group of values excluding the outliers, which are shown as dots. Groups with statistically significant differences ( P
    Figure Legend Snippet: Mutation of the YxxΦ and KVHVQ motifs of PEDV S protein altered the expression of S proteins on the cell surface and syncytium formation. (A) IF staining of S proteins expressed on cell surface, or total (surface and intracellular) S proteins (Total S). Vero cells were fixed by 4% formaldehyde at 12 h after transfection with plasmid DNA. Without permeabilization, surface S proteins were stained with guinea pig antiserum GP17 against PEDV S1 followed by Alexa Fluor 488 (AF488)-conjugated goat anti-guinea pig IgG. Total S proteins were observed under the mCherry channel. Scale bar: 30 μm. (B) The fluorescent intensities of the total S proteins (mCherry) were measured on 50 individual cells of each sample. NS, no statistically significant difference ( P > 0.05). (C) Surface S/total S fluorescent intensity ratios (AF488/mCherry) were calculated based on 50 individual cells of each sample. (D) Syncytia induced by mCherry-tagged WT S proteins and their mutants. At 6 h after transfection with the plasmid DNA, Vero cells were cultured in medium containing trypsin (10 μg/ml) for an additional 6 h. Cells were fixed with methanol and total S proteins were detected by IF staining with the antiserum GP17 as described above. Nuclei were stained with DAPI. Scale bar: 50 μm. (E) Nuclei in each syncytium were counted based on 200 syncytia for each sample. Values in panels B, C, and E are shown in box-whisker plots. The boxes indicate interquartile ranges in different groups, the lines in the boxes represent median values, and the whiskers show the range of a group of values excluding the outliers, which are shown as dots. Groups with statistically significant differences ( P

    Techniques Used: Mutagenesis, Expressing, Staining, Transfection, Plasmid Preparation, Cell Culture, Whisker Assay

    IF staining of S and M proteins coexpressed in Vero cells. (A) IF staining of surface S and M proteins in the Vero cells expressing S and M simultaneously or only S proteins at 12 hpt. The total S proteins (mCherry, red), M proteins (green), and cell surface S proteins (cyan) were visualized. Scale bar: 10 μm. In the images of the WT1, Δ10aa, and YA group, S-expressing cells with or without M protein expression are included. For the Δ5aa-M group, filled arrows indicate surface S (cyan) colocalized with S (mCherry, red) and M (green) and empty arrows indicate surface S (cyan) colocalized with S (mCherry, red). (B) Colocalization coefficients (PCC) between S and M proteins in the Vero cells. Ten to 18 cells in each group were measured and analyzed by one-way ANOVA and Tukey’s test. (C) Two sets of Vero cells were transfected with the individual S plasmid alone or cotransfected with the S and M plasmids. Surface S/total S fluorescent intensity ratios (AF647:mCherry) were calculated based on 10 to 20 individual cells for the cells coexpressing S and M or transfected with S alone. Values in panel B are shown in a box-whisker plot and were analyzed by one-way ANOVA followed by Tukey’s comparison test. In panel C, values in the group coexpressing S and M and the S-only group in each plasmid DNA are shown as means ± SDs and were analyzed by Student’s t test. *, P
    Figure Legend Snippet: IF staining of S and M proteins coexpressed in Vero cells. (A) IF staining of surface S and M proteins in the Vero cells expressing S and M simultaneously or only S proteins at 12 hpt. The total S proteins (mCherry, red), M proteins (green), and cell surface S proteins (cyan) were visualized. Scale bar: 10 μm. In the images of the WT1, Δ10aa, and YA group, S-expressing cells with or without M protein expression are included. For the Δ5aa-M group, filled arrows indicate surface S (cyan) colocalized with S (mCherry, red) and M (green) and empty arrows indicate surface S (cyan) colocalized with S (mCherry, red). (B) Colocalization coefficients (PCC) between S and M proteins in the Vero cells. Ten to 18 cells in each group were measured and analyzed by one-way ANOVA and Tukey’s test. (C) Two sets of Vero cells were transfected with the individual S plasmid alone or cotransfected with the S and M plasmids. Surface S/total S fluorescent intensity ratios (AF647:mCherry) were calculated based on 10 to 20 individual cells for the cells coexpressing S and M or transfected with S alone. Values in panel B are shown in a box-whisker plot and were analyzed by one-way ANOVA followed by Tukey’s comparison test. In panel C, values in the group coexpressing S and M and the S-only group in each plasmid DNA are shown as means ± SDs and were analyzed by Student’s t test. *, P

    Techniques Used: Staining, Expressing, Periodic Counter-current Chromatography, Transfection, Plasmid Preparation, Whisker Assay

    IF staining of antibody-S protein uptake assay in Vero cells. At 24 h after transfection with the plasmid DNA, Vero cells were incubated with guinea pig anti-S1 antiserum GP17 at 4°C for 10 min. After washing with PBS three times, one set of cells was cultured at 37°C for 30 min to allow endocytosis. Another set of cells was kept at 4°C for 30 min as the control group. Cells were fixed with 4% formaldehyde without permeabilization. Surface S proteins were stained with mouse anti-S2 monoclonal antibody SD129-5 and goat anti-mouse AF647-conjugated secondary antibodies (red). Then the cells were permeabilized with Triton X-100 and stained with goat anti-guinea pig AF488-conjugated secondary antibodies (green). Nuclei were stained with DAPI (blue). Cells were randomly selected, and images were taken by using a Leica TCS SP6 confocal microscope. Scale bar: 10 μm.
    Figure Legend Snippet: IF staining of antibody-S protein uptake assay in Vero cells. At 24 h after transfection with the plasmid DNA, Vero cells were incubated with guinea pig anti-S1 antiserum GP17 at 4°C for 10 min. After washing with PBS three times, one set of cells was cultured at 37°C for 30 min to allow endocytosis. Another set of cells was kept at 4°C for 30 min as the control group. Cells were fixed with 4% formaldehyde without permeabilization. Surface S proteins were stained with mouse anti-S2 monoclonal antibody SD129-5 and goat anti-mouse AF647-conjugated secondary antibodies (red). Then the cells were permeabilized with Triton X-100 and stained with goat anti-guinea pig AF488-conjugated secondary antibodies (green). Nuclei were stained with DAPI (blue). Cells were randomly selected, and images were taken by using a Leica TCS SP6 confocal microscope. Scale bar: 10 μm.

    Techniques Used: Staining, Transfection, Plasmid Preparation, Incubation, Cell Culture, Microscopy

    3) Product Images from "Deletion of both the Tyrosine-Based Endocytosis Signal and the Endoplasmic Reticulum Retrieval Signal in the Cytoplasmic Tail of Spike Protein Attenuates Porcine Epidemic Diarrhea Virus in Pigs"

    Article Title: Deletion of both the Tyrosine-Based Endocytosis Signal and the Endoplasmic Reticulum Retrieval Signal in the Cytoplasmic Tail of Spike Protein Attenuates Porcine Epidemic Diarrhea Virus in Pigs

    Journal: Journal of Virology

    doi: 10.1128/JVI.01758-18

    IF staining of antibody-S protein uptake assay in Vero cells. At 24 h after transfection with the plasmid DNA, Vero cells were incubated with guinea pig anti-S1 antiserum GP17 at 4°C for 10 min. After washing with PBS three times, one set of cells was cultured at 37°C for 30 min to allow endocytosis. Another set of cells was kept at 4°C for 30 min as the control group. Cells were fixed with 4% formaldehyde without permeabilization. Surface S proteins were stained with mouse anti-S2 monoclonal antibody SD129-5 and goat anti-mouse AF647-conjugated secondary antibodies (red). Then the cells were permeabilized with Triton X-100 and stained with goat anti-guinea pig AF488-conjugated secondary antibodies (green). Nuclei were stained with DAPI (blue). Cells were randomly selected, and images were taken by using a Leica TCS SP6 confocal microscope. Scale bar: 10 μm.
    Figure Legend Snippet: IF staining of antibody-S protein uptake assay in Vero cells. At 24 h after transfection with the plasmid DNA, Vero cells were incubated with guinea pig anti-S1 antiserum GP17 at 4°C for 10 min. After washing with PBS three times, one set of cells was cultured at 37°C for 30 min to allow endocytosis. Another set of cells was kept at 4°C for 30 min as the control group. Cells were fixed with 4% formaldehyde without permeabilization. Surface S proteins were stained with mouse anti-S2 monoclonal antibody SD129-5 and goat anti-mouse AF647-conjugated secondary antibodies (red). Then the cells were permeabilized with Triton X-100 and stained with goat anti-guinea pig AF488-conjugated secondary antibodies (green). Nuclei were stained with DAPI (blue). Cells were randomly selected, and images were taken by using a Leica TCS SP6 confocal microscope. Scale bar: 10 μm.

    Techniques Used: Staining, Transfection, Plasmid Preparation, Incubation, Cell Culture, Microscopy

    4) Product Images from "Stromal Cells Maintain Immune Cell Homeostasis in Adipose Tissue via Production of Interleukin-33"

    Article Title: Stromal Cells Maintain Immune Cell Homeostasis in Adipose Tissue via Production of Interleukin-33

    Journal: Science immunology

    doi: 10.1126/sciimmunol.aax0416

    IL-33 controls adipose tissue expansion and immunological homeostasis in short-term HFD feeding. ( A - B ) Wild type mice were fed with high-fat diet (HFD) or control diet (CD) up to 7 days (d). ( A ) Representative dot plot of Ki67 staining in ASPCs (defined as live lin − (CD31 − CD45 − Ter119 − )) PDGFRα + Sca-1 + cells) on day 7. ( B ) Proportion of proliferating cells (Ki67 + ) cells among total ASPCs from indicated WAT depots. (n = 3) ( C ) Expression of Il33 and Mcp1 in sort-purified ASPCs isolated from ewat of wild type mice (WT) on CD or HFD for 3 days as determined by qRT-PCR. (n = 3) ( D to I ) WT or Il33 −/− mice were fed with CD or HFD and sacrificed for analysis after 3 days. ( D ) Body weight change as a proportion (%) of initial weight (day 0). Data compiled from two individual experiments. (n ≥ 7) ( E ) Proportion of ewat mass as proportion (%) of body weight. Data compiled from two individual experiments. (n ≥ 7) ( F ) Proportion of Ki67 + and adipocyte progenitor cells (CD24 + ) among total ewat ASPCs. ( G ) Mean fluorescence intensity (MFI) of forward scatter (FSC) and side scatter (SSC) of ewat ASPCs. ( H and I ) Quantification of macrophages ( H ) and dendritic cells (DCs) ( I ) in ewat. (n ≥ 4). Mean ± SEM, * P
    Figure Legend Snippet: IL-33 controls adipose tissue expansion and immunological homeostasis in short-term HFD feeding. ( A - B ) Wild type mice were fed with high-fat diet (HFD) or control diet (CD) up to 7 days (d). ( A ) Representative dot plot of Ki67 staining in ASPCs (defined as live lin − (CD31 − CD45 − Ter119 − )) PDGFRα + Sca-1 + cells) on day 7. ( B ) Proportion of proliferating cells (Ki67 + ) cells among total ASPCs from indicated WAT depots. (n = 3) ( C ) Expression of Il33 and Mcp1 in sort-purified ASPCs isolated from ewat of wild type mice (WT) on CD or HFD for 3 days as determined by qRT-PCR. (n = 3) ( D to I ) WT or Il33 −/− mice were fed with CD or HFD and sacrificed for analysis after 3 days. ( D ) Body weight change as a proportion (%) of initial weight (day 0). Data compiled from two individual experiments. (n ≥ 7) ( E ) Proportion of ewat mass as proportion (%) of body weight. Data compiled from two individual experiments. (n ≥ 7) ( F ) Proportion of Ki67 + and adipocyte progenitor cells (CD24 + ) among total ewat ASPCs. ( G ) Mean fluorescence intensity (MFI) of forward scatter (FSC) and side scatter (SSC) of ewat ASPCs. ( H and I ) Quantification of macrophages ( H ) and dendritic cells (DCs) ( I ) in ewat. (n ≥ 4). Mean ± SEM, * P

    Techniques Used: Mouse Assay, Staining, Expressing, Purification, Isolation, Quantitative RT-PCR, Fluorescence

    Adipose stem and progenitor cell-derived IL-33 controls ILC2 activity. ( A ) Representative histogram and dot plots showing ST2 + cells in the lymphocyte population first gated on live CD45 + cells. ( B ) from the small intestinal lamina propria (SI LP) and epididymal WAT (ewat). ( B and C) Representative histogram ( B ) and bar graph ( C ) showing mean fluorescence intensity (MFI) of ST2 on ILC2s from ewat and SI LP. (n = 6) ( D ) Schematic indicating experimental design for E and F. ( E ) Sort-purified ILC2s from wild type (WT) or Il1rl1 −/− mouse visceral WAT (pooled ewat and mwat) ILC2s were cultured in ASPC-conditioned (ASPC) or control media (Ctrl) and IL-5 secretion was analyzed by ELISA after 48 h. (n = 3) ( F) Sort-purified ILC2s from visceral WAT or SI LP were cultured in ASPC-conditioned or control media (Ctrl) and IL-5 and IL-13 secretion was analyzed by multiplex bead-based assay after 48 h. (n = 3) ( G ) Schematic indicating experimental design. Sort-purified ASPCs from wild type (WT) or Il33 −/− mice were injected into the peritoneum of Il33 −/− mice and immunological analysis ( H - J ) performed after six weeks in visceral adipose compartments. (H) ILC2 numbers, ( I ) proportions of IL-5 + and IL-13 + ILC2s, and ( J ) eosinophil quantification. (n = 4). Mean ± SEM, * P
    Figure Legend Snippet: Adipose stem and progenitor cell-derived IL-33 controls ILC2 activity. ( A ) Representative histogram and dot plots showing ST2 + cells in the lymphocyte population first gated on live CD45 + cells. ( B ) from the small intestinal lamina propria (SI LP) and epididymal WAT (ewat). ( B and C) Representative histogram ( B ) and bar graph ( C ) showing mean fluorescence intensity (MFI) of ST2 on ILC2s from ewat and SI LP. (n = 6) ( D ) Schematic indicating experimental design for E and F. ( E ) Sort-purified ILC2s from wild type (WT) or Il1rl1 −/− mouse visceral WAT (pooled ewat and mwat) ILC2s were cultured in ASPC-conditioned (ASPC) or control media (Ctrl) and IL-5 secretion was analyzed by ELISA after 48 h. (n = 3) ( F) Sort-purified ILC2s from visceral WAT or SI LP were cultured in ASPC-conditioned or control media (Ctrl) and IL-5 and IL-13 secretion was analyzed by multiplex bead-based assay after 48 h. (n = 3) ( G ) Schematic indicating experimental design. Sort-purified ASPCs from wild type (WT) or Il33 −/− mice were injected into the peritoneum of Il33 −/− mice and immunological analysis ( H - J ) performed after six weeks in visceral adipose compartments. (H) ILC2 numbers, ( I ) proportions of IL-5 + and IL-13 + ILC2s, and ( J ) eosinophil quantification. (n = 4). Mean ± SEM, * P

    Techniques Used: Derivative Assay, Activity Assay, Fluorescence, Purification, Cell Culture, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Bead-based Assay, Mouse Assay, Injection

    5) Product Images from "Optimized Generation of Functional Neutrophils and Macrophages from Patient-Specific Induced Pluripotent Stem Cells: Ex Vivo Models of X0-Linked, AR220- and AR470- Chronic Granulomatous Diseases"

    Article Title: Optimized Generation of Functional Neutrophils and Macrophages from Patient-Specific Induced Pluripotent Stem Cells: Ex Vivo Models of X0-Linked, AR220- and AR470- Chronic Granulomatous Diseases

    Journal: BioResearch Open Access

    doi: 10.1089/biores.2014.0045

    Functional characterization of neutrophils and macrophages derived from WT and CGD-iPSCs. (A) Expression of the NADPH oxidase subunits gp91 phox and p22 phox in neutrophils and macrophages derived from WT and CGD-iPSC lines. Cells were incubated with the NOX2 monoclonal antibody 7D5 or the monoclonal antibody against p22 phox (clone 44.1) respectively, and then with a phycoerythrin-conjugated anti-mouse immunoglobin G (IgG) (H+L). Mouse IgG1 isotype was used as an irrelevant monoclonal antibody. Results are representative of three independent experiments. (B) Nitro blue tetrazolium (NBT) reduction assay on opsonized latex beads-activated WT or CGD iPSC-derived neutrophils and macrophages ( n =2). Reactive oxygen species (ROS)-mediated NBT reduction is shown as blue formazan precipitates in WT neutrophils and macrophages only (scale bars: 20 μm). (C) Images of dihydrorhodamine-1,2,3 (DHR) dot plot showing ROS production by phorbol 12-myristate 13-acetate (PMA)-stimulated neutrophils derived from WT and AR22 0 -CGD-iPSCs. (D) Kinetics of H 2 O 2 production measured by luminol-enhanced chemiluminescence assay during 60 min after stimulation of WT and CGD neutrophils ( upper panels ) or macrophages ( lower panels ) with phorbol 12-myristate 13-acetate (PMA) or opsonized zymosan (ZOPS) particles. Inserts represent enlargement of the Relative Luminescence Unit Scale for neutrophils. Results are representative of three independent experiments.
    Figure Legend Snippet: Functional characterization of neutrophils and macrophages derived from WT and CGD-iPSCs. (A) Expression of the NADPH oxidase subunits gp91 phox and p22 phox in neutrophils and macrophages derived from WT and CGD-iPSC lines. Cells were incubated with the NOX2 monoclonal antibody 7D5 or the monoclonal antibody against p22 phox (clone 44.1) respectively, and then with a phycoerythrin-conjugated anti-mouse immunoglobin G (IgG) (H+L). Mouse IgG1 isotype was used as an irrelevant monoclonal antibody. Results are representative of three independent experiments. (B) Nitro blue tetrazolium (NBT) reduction assay on opsonized latex beads-activated WT or CGD iPSC-derived neutrophils and macrophages ( n =2). Reactive oxygen species (ROS)-mediated NBT reduction is shown as blue formazan precipitates in WT neutrophils and macrophages only (scale bars: 20 μm). (C) Images of dihydrorhodamine-1,2,3 (DHR) dot plot showing ROS production by phorbol 12-myristate 13-acetate (PMA)-stimulated neutrophils derived from WT and AR22 0 -CGD-iPSCs. (D) Kinetics of H 2 O 2 production measured by luminol-enhanced chemiluminescence assay during 60 min after stimulation of WT and CGD neutrophils ( upper panels ) or macrophages ( lower panels ) with phorbol 12-myristate 13-acetate (PMA) or opsonized zymosan (ZOPS) particles. Inserts represent enlargement of the Relative Luminescence Unit Scale for neutrophils. Results are representative of three independent experiments.

    Techniques Used: Functional Assay, Derivative Assay, Expressing, Incubation, Chemiluminescence Immunoassay

    6) Product Images from "Deletion of both the Tyrosine-Based Endocytosis Signal and the Endoplasmic Reticulum Retrieval Signal in the Cytoplasmic Tail of Spike Protein Attenuates Porcine Epidemic Diarrhea Virus in Pigs"

    Article Title: Deletion of both the Tyrosine-Based Endocytosis Signal and the Endoplasmic Reticulum Retrieval Signal in the Cytoplasmic Tail of Spike Protein Attenuates Porcine Epidemic Diarrhea Virus in Pigs

    Journal: Journal of Virology

    doi: 10.1128/JVI.01758-18

    IF staining of antibody-S protein uptake assay in Vero cells. At 24 h after transfection with the plasmid DNA, Vero cells were incubated with guinea pig anti-S1 antiserum GP17 at 4°C for 10 min. After washing with PBS three times, one set of cells was cultured at 37°C for 30 min to allow endocytosis. Another set of cells was kept at 4°C for 30 min as the control group. Cells were fixed with 4% formaldehyde without permeabilization. Surface S proteins were stained with mouse anti-S2 monoclonal antibody SD129-5 and goat anti-mouse AF647-conjugated secondary antibodies (red). Then the cells were permeabilized with Triton X-100 and stained with goat anti-guinea pig AF488-conjugated secondary antibodies (green). Nuclei were stained with DAPI (blue). Cells were randomly selected, and images were taken by using a Leica TCS SP6 confocal microscope. Scale bar: 10 μm.
    Figure Legend Snippet: IF staining of antibody-S protein uptake assay in Vero cells. At 24 h after transfection with the plasmid DNA, Vero cells were incubated with guinea pig anti-S1 antiserum GP17 at 4°C for 10 min. After washing with PBS three times, one set of cells was cultured at 37°C for 30 min to allow endocytosis. Another set of cells was kept at 4°C for 30 min as the control group. Cells were fixed with 4% formaldehyde without permeabilization. Surface S proteins were stained with mouse anti-S2 monoclonal antibody SD129-5 and goat anti-mouse AF647-conjugated secondary antibodies (red). Then the cells were permeabilized with Triton X-100 and stained with goat anti-guinea pig AF488-conjugated secondary antibodies (green). Nuclei were stained with DAPI (blue). Cells were randomly selected, and images were taken by using a Leica TCS SP6 confocal microscope. Scale bar: 10 μm.

    Techniques Used: Staining, Transfection, Plasmid Preparation, Incubation, Cell Culture, Microscopy

    7) Product Images from "Transplantation of induced neural stem cells (iNSCs) into chronically demyelinated corpus callosum ameliorates motor deficits"

    Article Title: Transplantation of induced neural stem cells (iNSCs) into chronically demyelinated corpus callosum ameliorates motor deficits

    Journal: Acta Neuropathologica Communications

    doi: 10.1186/s40478-020-00960-3

    Transplanted iNSCs are localized mainly in white matter and express markers of oligodendrocyte and astrocyte differentiation. a: The relative distribution of iNSCs in neuroanatomical regions adjacent to the CC target site. iNSCs quantified by direct detection of GFP expression in tissue sections combined from analysis of mice in the MRI and behavior cohorts for neuropathology and iNSC cell type identification. b-d: Quantification of differentiation of transplanted iNSCs in white matter based on direct visualization of GFP expression and co-labeling with markers for neural stem/progenitor cells (Sox2), oligodendrocyte lineage cells (Olig2), or the astrocyte lineage (GFAP). Black fill shows counts of iNSC co-labeled for given cell marker, with upper bar section showing unlabeled iNSC counts. Percent co-labeling shown for each region. e-g: Examples of transplanted iNSCs expressing green fluorescent protein (GFP) along with nuclear Sox2 ( e ), nuclear Olig2 ( f ), or cytoplasmic GFAP ( g ). iNSC membranes labeled for GFP extend around cell bodies (pink arrows), along axons (white arrow), and to blood vessels (blue arrow). iNSCs were not identified as microglia in any sections analyzed with IBA1 immunolabeling. Microglia often contained autofluorescent lipofuscin granules ( f , yellow arrows). Tissue sections from mice of both the MRI ( n = 6) and behavior ( n = 11) cohorts were combined for the analysis of iNSC distribution. Differentiation studies included a subset of mice (Sox2 ( n = 6), Olig2 ( n = 9), and GFAP ( n = 14)) combined from MRI and behavior cohorts to generate contingency tables of total cell counts among sections analyzed for comparison using Fisher’s exact test. Scale bars E = 10 μm; F = 20 μm; G = 5 μm
    Figure Legend Snippet: Transplanted iNSCs are localized mainly in white matter and express markers of oligodendrocyte and astrocyte differentiation. a: The relative distribution of iNSCs in neuroanatomical regions adjacent to the CC target site. iNSCs quantified by direct detection of GFP expression in tissue sections combined from analysis of mice in the MRI and behavior cohorts for neuropathology and iNSC cell type identification. b-d: Quantification of differentiation of transplanted iNSCs in white matter based on direct visualization of GFP expression and co-labeling with markers for neural stem/progenitor cells (Sox2), oligodendrocyte lineage cells (Olig2), or the astrocyte lineage (GFAP). Black fill shows counts of iNSC co-labeled for given cell marker, with upper bar section showing unlabeled iNSC counts. Percent co-labeling shown for each region. e-g: Examples of transplanted iNSCs expressing green fluorescent protein (GFP) along with nuclear Sox2 ( e ), nuclear Olig2 ( f ), or cytoplasmic GFAP ( g ). iNSC membranes labeled for GFP extend around cell bodies (pink arrows), along axons (white arrow), and to blood vessels (blue arrow). iNSCs were not identified as microglia in any sections analyzed with IBA1 immunolabeling. Microglia often contained autofluorescent lipofuscin granules ( f , yellow arrows). Tissue sections from mice of both the MRI ( n = 6) and behavior ( n = 11) cohorts were combined for the analysis of iNSC distribution. Differentiation studies included a subset of mice (Sox2 ( n = 6), Olig2 ( n = 9), and GFAP ( n = 14)) combined from MRI and behavior cohorts to generate contingency tables of total cell counts among sections analyzed for comparison using Fisher’s exact test. Scale bars E = 10 μm; F = 20 μm; G = 5 μm

    Techniques Used: Expressing, Mouse Assay, Magnetic Resonance Imaging, Labeling, Marker, Immunolabeling

    Mouse induced neural stem cells (iNSCs) exhibit stem cell characteristics in vitro. a-b: In vitro, iNSCs form neurospheres ( a ) and express NSC markers Sox2 (red) and Nestin (pseudocolored green). c-e: Following differentiation of iNSCs, constitutively expressed green fluorescent protein (GFP) is visible in all cells ( c ) while cells differentiated along an astrocytic lineage exhibit immunolabeling for GFAP (red, c ). In addition, differentiation along neuronal (MAP2; red, d ) and oligodendroglial (O4; red, e ) lineages shows multipotency of iNSCs (GFP not shown). f: In non-differentiation conditions, iNSCs exhibit an exponential proliferation rate expected for stem cell growth based on quantification of each subculture after each passage. g: Quantification of iNSC multipotent differentiation from separately revived vials ( n = 4). Data are mean values ± sem. Scale bar A = 100 μm, B, D, E = 20 μm, C = 40 μm
    Figure Legend Snippet: Mouse induced neural stem cells (iNSCs) exhibit stem cell characteristics in vitro. a-b: In vitro, iNSCs form neurospheres ( a ) and express NSC markers Sox2 (red) and Nestin (pseudocolored green). c-e: Following differentiation of iNSCs, constitutively expressed green fluorescent protein (GFP) is visible in all cells ( c ) while cells differentiated along an astrocytic lineage exhibit immunolabeling for GFAP (red, c ). In addition, differentiation along neuronal (MAP2; red, d ) and oligodendroglial (O4; red, e ) lineages shows multipotency of iNSCs (GFP not shown). f: In non-differentiation conditions, iNSCs exhibit an exponential proliferation rate expected for stem cell growth based on quantification of each subculture after each passage. g: Quantification of iNSC multipotent differentiation from separately revived vials ( n = 4). Data are mean values ± sem. Scale bar A = 100 μm, B, D, E = 20 μm, C = 40 μm

    Techniques Used: In Vitro, Immunolabeling

    Related Articles

    Transfection:

    Article Title: Identification of Human Junctional Adhesion Molecule 1 as a Functional Receptor for the Hom-1 Calicivirus on Human Cells
    Article Snippet: .. Two days after transfection, cells were lysed with 1.4 ml of lysis buffer containing 50 mM Tris (pH 7.5), 100 mM NaCl, 5 mM MgCl2 , 1% Triton X-100, 50% glycerol, and 1× Halt protease inhibitor cocktail (Thermo Fisher Scientific. ..

    Protease Inhibitor:

    Article Title: Identification of Human Junctional Adhesion Molecule 1 as a Functional Receptor for the Hom-1 Calicivirus on Human Cells
    Article Snippet: .. Two days after transfection, cells were lysed with 1.4 ml of lysis buffer containing 50 mM Tris (pH 7.5), 100 mM NaCl, 5 mM MgCl2 , 1% Triton X-100, 50% glycerol, and 1× Halt protease inhibitor cocktail (Thermo Fisher Scientific. ..

    Infection:

    Article Title: Identification and Characterization of Thymus LIM Protein: Targeted Disruption Reduces Thymus Cellularity
    Article Snippet: .. Uninfected and infected cells were fixed in 4% formalin, permeabilized in 0.1% Triton X-100, and stained using affinity-purified anti-TLP at 1:100 and rhodamine-phalloidin (Molecular Probes, Eugene, Oreg.) at 1:600. .. Fluorescein isothiocyanate (FITC)-conjugated anti-rabbit IgG was used to visualize anti-TLP staining. ) with Bio-Rad plugins.

    Incubation:

    Article Title: Proteins of nucleotide and base excision repair pathways interact in mitochondria to protect from loss of subcutaneous fat, a hallmark of aging
    Article Snippet: .. Slides were rinsed (0.25% Triton X-100 in PBS with 1% donkey serum) three times and incubated with YOPRO-1 (Invitrogen) for nuclear staining. ..

    other:

    Article Title: Functional Deficiencies and a Reduced Response to Calcium in the Flagellum of Mouse Sperm Lacking SPAG16L 1
    Article Snippet: Adenosine triphosphate-induced motility and sliding of filaments in mammalian sperm extracted with Triton X-100.

    BAC Assay:

    Article Title: Antimicrobial Activity of Nanoemulsion on Cariogenic Planktonic and Biofilm Organisms
    Article Snippet: .. The grown biofilm was treated with nanoemulsion, or with 1% CPC, 10% Triton X-100, or a combination of 1% CPC and 10% Triton X-100 for 1 min. Biofilms were stained with L 7012 LIVE/DEAD® Bac Light™ Bacterial Viability Kit from Molecular Probes Inc. (Eugene, OR) as described by Neu and Lawrence [ ]. .. The staining solution, containing two components SYTO9 and propidium iodide, was mixed in equal quantities and applied to the wells for 15 min.

    Staining:

    Article Title: p21-Activated Kinases Are Required for Transformation in a Cell-Based Model of Neurofibromatosis Type 2
    Article Snippet: .. Immunofluorescence Cells were plated on glass coverslips in 6-well culture plates and fixed in 4% paraformaldehyde for 10 min, permeabilized with 0.2% Triton X-100 for 5 min and blocked with 1% BSA in PBS for 30 min. Filamentous actin and nuclei were visualized by staining with Rhodamine-phalloidin (Molecular Probe) and DAPI (Sigma), respectively. .. Images were observed and captured on an inverted phase/fluorescence microscope (Nikon TE300).

    Article Title: Autophagy-Associated Proteins Control Ebola Virus Internalization Into Host Cells
    Article Snippet: .. The cells grown on slides were fixed, permeabilized with 0.1% Triton X-100 for 10 minutes, blocked with 5% goat serum (Thermo Fisher), stained with anti-Ankfy1 antibody overnight and then with Alexa Fluor 546–conjugated anti-rabbit secondary antibody and HCS CellMask blue stain (Thermo Fisher). .. Images were acquired using a Nikon Ti-Eclipse microscope (Nikon, Tokyo, Japan).

    Article Title: Identification and Characterization of Thymus LIM Protein: Targeted Disruption Reduces Thymus Cellularity
    Article Snippet: .. Uninfected and infected cells were fixed in 4% formalin, permeabilized in 0.1% Triton X-100, and stained using affinity-purified anti-TLP at 1:100 and rhodamine-phalloidin (Molecular Probes, Eugene, Oreg.) at 1:600. .. Fluorescein isothiocyanate (FITC)-conjugated anti-rabbit IgG was used to visualize anti-TLP staining. ) with Bio-Rad plugins.

    Article Title: Proteins of nucleotide and base excision repair pathways interact in mitochondria to protect from loss of subcutaneous fat, a hallmark of aging
    Article Snippet: .. Slides were rinsed (0.25% Triton X-100 in PBS with 1% donkey serum) three times and incubated with YOPRO-1 (Invitrogen) for nuclear staining. ..

    Article Title: Antimicrobial Activity of Nanoemulsion on Cariogenic Planktonic and Biofilm Organisms
    Article Snippet: .. The grown biofilm was treated with nanoemulsion, or with 1% CPC, 10% Triton X-100, or a combination of 1% CPC and 10% Triton X-100 for 1 min. Biofilms were stained with L 7012 LIVE/DEAD® Bac Light™ Bacterial Viability Kit from Molecular Probes Inc. (Eugene, OR) as described by Neu and Lawrence [ ]. .. The staining solution, containing two components SYTO9 and propidium iodide, was mixed in equal quantities and applied to the wells for 15 min.

    Lysis:

    Article Title: Identification of Human Junctional Adhesion Molecule 1 as a Functional Receptor for the Hom-1 Calicivirus on Human Cells
    Article Snippet: .. Two days after transfection, cells were lysed with 1.4 ml of lysis buffer containing 50 mM Tris (pH 7.5), 100 mM NaCl, 5 mM MgCl2 , 1% Triton X-100, 50% glycerol, and 1× Halt protease inhibitor cocktail (Thermo Fisher Scientific. ..

    Affinity Purification:

    Article Title: Identification and Characterization of Thymus LIM Protein: Targeted Disruption Reduces Thymus Cellularity
    Article Snippet: .. Uninfected and infected cells were fixed in 4% formalin, permeabilized in 0.1% Triton X-100, and stained using affinity-purified anti-TLP at 1:100 and rhodamine-phalloidin (Molecular Probes, Eugene, Oreg.) at 1:600. .. Fluorescein isothiocyanate (FITC)-conjugated anti-rabbit IgG was used to visualize anti-TLP staining. ) with Bio-Rad plugins.

    Immunofluorescence:

    Article Title: p21-Activated Kinases Are Required for Transformation in a Cell-Based Model of Neurofibromatosis Type 2
    Article Snippet: .. Immunofluorescence Cells were plated on glass coverslips in 6-well culture plates and fixed in 4% paraformaldehyde for 10 min, permeabilized with 0.2% Triton X-100 for 5 min and blocked with 1% BSA in PBS for 30 min. Filamentous actin and nuclei were visualized by staining with Rhodamine-phalloidin (Molecular Probe) and DAPI (Sigma), respectively. .. Images were observed and captured on an inverted phase/fluorescence microscope (Nikon TE300).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher af647 conjugated goat polyclonal anti mouse igg
    Af647 Conjugated Goat Polyclonal Anti Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/af647 conjugated goat polyclonal anti mouse igg/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    af647 conjugated goat polyclonal anti mouse igg - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    Image Search Results