antibodies t7 expression system  (Thermo Fisher)


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    Name:
    pRSET A B C Bacterial Expression Vectors
    Description:
    The pRSET vector is designed for high level prokaryotic expression controlled by the strong bacteriophage T7 promoter Expression is induced by the production of T7 RNA polymerase in BL21 DE3 E coli These cells also produce T7 lysozyme to reduce basal expression of target genes The pRSET vector offers • Bacteriophage T7 promoter for high level expression• T7 gene 10 sequence to provide protein stability• N terminal polyhistidine 6xHis tag for rapid purification with nickel chelating resin and detection with an Anti HisG Antibody• N terminal Xpress epitope for detection with the Anti Xpress Antibody• Enterokinase cleavage site for removal of fusion tagA set of three vectors is provided A B and C Each has the N terminal tag coding sequence in a different reading frame relative to the multiple cloning site to simplify in frame cloning of your gene
    Catalog Number:
    v35120
    Price:
    None
    Applications:
    Bacterial Expression|Protein Biology|Protein Expression|T7 Bacterial Expression
    Category:
    DNA Vectors Clones Purified Nucleic Acids Libraries
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    Structured Review

    Thermo Fisher antibodies t7 expression system
    The pRSET vector is designed for high level prokaryotic expression controlled by the strong bacteriophage T7 promoter Expression is induced by the production of T7 RNA polymerase in BL21 DE3 E coli These cells also produce T7 lysozyme to reduce basal expression of target genes The pRSET vector offers • Bacteriophage T7 promoter for high level expression• T7 gene 10 sequence to provide protein stability• N terminal polyhistidine 6xHis tag for rapid purification with nickel chelating resin and detection with an Anti HisG Antibody• N terminal Xpress epitope for detection with the Anti Xpress Antibody• Enterokinase cleavage site for removal of fusion tagA set of three vectors is provided A B and C Each has the N terminal tag coding sequence in a different reading frame relative to the multiple cloning site to simplify in frame cloning of your gene
    https://www.bioz.com/result/antibodies t7 expression system/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies t7 expression system - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch
    Article Snippet: .. The PCR product was digested with restriction enzymes and cloned into similarly digested pRSET-A bacterial expression vectors (Invitrogen). .. The plasmids were prepared in E. coli JM109 cells (Stratagene).

    Article Title: Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch
    Article Snippet: .. The PCR products were digested with restriction enzymes and cloned into similarly digested pRSET-A bacterial expression vectors (Invitrogen). .. The plasmids were prepared in Escherichia coli JM109 cells (Promega).

    Article Title: Formation of Spindle Poles by Dynein/Dynactin-Dependent Transport of Numa
    Article Snippet: .. The dynamitin PCR product was cloned in the vector pCR-TM2.1, excised using EcoR1, and cloned into the bacterial expression vector pRSET A (both from Invitrogen). .. Bacterial fusion protein was isolated using 8M urea and dialyzed against PBS before the assay.

    CtB Assay:

    Article Title: Induction of Indoleamine 2, 3-Dioxygenase in Human Dendritic Cells by a Cholera Toxin B Subunit—Proinsulin Vaccine
    Article Snippet: .. Expression vector pRSET-CTB-INS was transformed into the E . coli producer strain BL21 (DE3) pLysS (Invitrogen, Carlsbad, CA) for production and isolation of milligram amounts of the CTB-INS protein for further experiments [ ]. .. Synthesis and isolation of CTB-INS fusion protein The E . coli strain BL21 transformed with pRSET-CTB-INS [ ] was grown overnight at 37°C in a 2.0 ml Luria Broth (LB) shake culture containing 100 μg/ml ampicillin for selection of transformed cells.

    Article Title: Suppression of Dendritic Cell Activation by Diabetes Autoantigens Linked to the Cholera Toxin B Subunit
    Article Snippet: .. The expression vector PRSET-CTB-INS containing the gene encoding the CTB-INS fusion protein was introduced into E. coli producer strain BL21 (DE3)pLysS (Invitrogen, Carlsbad, CA), for nickel affinity column isolation of the recombinant protein ( ). .. The CTB-INS transformed E. coli strain BL-21, was grown in 250 ml Luria Broth (LB) medium containing ampicillin (100mg/ml).

    Construct:

    Article Title: A Non-Invasive Tool for Real-Time Measurement of Sulfate in Living Cells
    Article Snippet: .. In Bacteria The ECFP_sbp_mVenus chimeric construct was ligated with the pRSET-B expression vector (Invitrogen, USA) and was transformed into the BL21-CodonPlus (DE3) strain of E. coli. .. A single transformed colony was grown on LB medium for three days at 20 °C in the dark to prevent fluorescent protein from photobleaching.

    Affinity Column:

    Article Title: Suppression of Dendritic Cell Activation by Diabetes Autoantigens Linked to the Cholera Toxin B Subunit
    Article Snippet: .. The expression vector PRSET-CTB-INS containing the gene encoding the CTB-INS fusion protein was introduced into E. coli producer strain BL21 (DE3)pLysS (Invitrogen, Carlsbad, CA), for nickel affinity column isolation of the recombinant protein ( ). .. The CTB-INS transformed E. coli strain BL-21, was grown in 250 ml Luria Broth (LB) medium containing ampicillin (100mg/ml).

    Expressing:

    Article Title: Induction of Indoleamine 2, 3-Dioxygenase in Human Dendritic Cells by a Cholera Toxin B Subunit—Proinsulin Vaccine
    Article Snippet: .. Expression vector pRSET-CTB-INS was transformed into the E . coli producer strain BL21 (DE3) pLysS (Invitrogen, Carlsbad, CA) for production and isolation of milligram amounts of the CTB-INS protein for further experiments [ ]. .. Synthesis and isolation of CTB-INS fusion protein The E . coli strain BL21 transformed with pRSET-CTB-INS [ ] was grown overnight at 37°C in a 2.0 ml Luria Broth (LB) shake culture containing 100 μg/ml ampicillin for selection of transformed cells.

    Article Title: Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch
    Article Snippet: .. The PCR product was digested with restriction enzymes and cloned into similarly digested pRSET-A bacterial expression vectors (Invitrogen). .. The plasmids were prepared in E. coli JM109 cells (Stratagene).

    Article Title: GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28
    Article Snippet: .. The PCR product was digested and subcloned into the pRSET–C bacterial expression vector (Invitrogen) to obtain a His6 N–terminal-tagged recombinant GATE–16 protein. .. The pRSET–C plasmid containing GATE–16 was transformed into E.coli BL–21.

    Article Title: A Non-Invasive Tool for Real-Time Measurement of Sulfate in Living Cells
    Article Snippet: .. In Bacteria The ECFP_sbp_mVenus chimeric construct was ligated with the pRSET-B expression vector (Invitrogen, USA) and was transformed into the BL21-CodonPlus (DE3) strain of E. coli. .. A single transformed colony was grown on LB medium for three days at 20 °C in the dark to prevent fluorescent protein from photobleaching.

    Article Title: Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch
    Article Snippet: .. The PCR products were digested with restriction enzymes and cloned into similarly digested pRSET-A bacterial expression vectors (Invitrogen). .. The plasmids were prepared in Escherichia coli JM109 cells (Promega).

    Article Title: Formation of Spindle Poles by Dynein/Dynactin-Dependent Transport of Numa
    Article Snippet: .. The dynamitin PCR product was cloned in the vector pCR-TM2.1, excised using EcoR1, and cloned into the bacterial expression vector pRSET A (both from Invitrogen). .. Bacterial fusion protein was isolated using 8M urea and dialyzed against PBS before the assay.

    Article Title: Biophysical characterization and crystal structure of the Feline Immunodeficiency Virus p15 matrix protein
    Article Snippet: .. The PCR products were digested with NdeI and Bgl2 and inserted into the MCS of the pRSET-B prokaryotic expression vector (Invitrogen) digested with the same enzymes. .. This creates the pRSET-p15 expression plasmid for our p15 constructs that encode proteins with a 6-His tag at the C-terminal end of the protein.

    Article Title: Suppression of Dendritic Cell Activation by Diabetes Autoantigens Linked to the Cholera Toxin B Subunit
    Article Snippet: .. The expression vector PRSET-CTB-INS containing the gene encoding the CTB-INS fusion protein was introduced into E. coli producer strain BL21 (DE3)pLysS (Invitrogen, Carlsbad, CA), for nickel affinity column isolation of the recombinant protein ( ). .. The CTB-INS transformed E. coli strain BL-21, was grown in 250 ml Luria Broth (LB) medium containing ampicillin (100mg/ml).

    Isolation:

    Article Title: Induction of Indoleamine 2, 3-Dioxygenase in Human Dendritic Cells by a Cholera Toxin B Subunit—Proinsulin Vaccine
    Article Snippet: .. Expression vector pRSET-CTB-INS was transformed into the E . coli producer strain BL21 (DE3) pLysS (Invitrogen, Carlsbad, CA) for production and isolation of milligram amounts of the CTB-INS protein for further experiments [ ]. .. Synthesis and isolation of CTB-INS fusion protein The E . coli strain BL21 transformed with pRSET-CTB-INS [ ] was grown overnight at 37°C in a 2.0 ml Luria Broth (LB) shake culture containing 100 μg/ml ampicillin for selection of transformed cells.

    Article Title: Suppression of Dendritic Cell Activation by Diabetes Autoantigens Linked to the Cholera Toxin B Subunit
    Article Snippet: .. The expression vector PRSET-CTB-INS containing the gene encoding the CTB-INS fusion protein was introduced into E. coli producer strain BL21 (DE3)pLysS (Invitrogen, Carlsbad, CA), for nickel affinity column isolation of the recombinant protein ( ). .. The CTB-INS transformed E. coli strain BL-21, was grown in 250 ml Luria Broth (LB) medium containing ampicillin (100mg/ml).

    Polymerase Chain Reaction:

    Article Title: Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch
    Article Snippet: .. The PCR product was digested with restriction enzymes and cloned into similarly digested pRSET-A bacterial expression vectors (Invitrogen). .. The plasmids were prepared in E. coli JM109 cells (Stratagene).

    Article Title: GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28
    Article Snippet: .. The PCR product was digested and subcloned into the pRSET–C bacterial expression vector (Invitrogen) to obtain a His6 N–terminal-tagged recombinant GATE–16 protein. .. The pRSET–C plasmid containing GATE–16 was transformed into E.coli BL–21.

    Article Title: Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch
    Article Snippet: .. The PCR products were digested with restriction enzymes and cloned into similarly digested pRSET-A bacterial expression vectors (Invitrogen). .. The plasmids were prepared in Escherichia coli JM109 cells (Promega).

    Article Title: Formation of Spindle Poles by Dynein/Dynactin-Dependent Transport of Numa
    Article Snippet: .. The dynamitin PCR product was cloned in the vector pCR-TM2.1, excised using EcoR1, and cloned into the bacterial expression vector pRSET A (both from Invitrogen). .. Bacterial fusion protein was isolated using 8M urea and dialyzed against PBS before the assay.

    Article Title: Biophysical characterization and crystal structure of the Feline Immunodeficiency Virus p15 matrix protein
    Article Snippet: .. The PCR products were digested with NdeI and Bgl2 and inserted into the MCS of the pRSET-B prokaryotic expression vector (Invitrogen) digested with the same enzymes. .. This creates the pRSET-p15 expression plasmid for our p15 constructs that encode proteins with a 6-His tag at the C-terminal end of the protein.

    Transformation Assay:

    Article Title: Induction of Indoleamine 2, 3-Dioxygenase in Human Dendritic Cells by a Cholera Toxin B Subunit—Proinsulin Vaccine
    Article Snippet: .. Expression vector pRSET-CTB-INS was transformed into the E . coli producer strain BL21 (DE3) pLysS (Invitrogen, Carlsbad, CA) for production and isolation of milligram amounts of the CTB-INS protein for further experiments [ ]. .. Synthesis and isolation of CTB-INS fusion protein The E . coli strain BL21 transformed with pRSET-CTB-INS [ ] was grown overnight at 37°C in a 2.0 ml Luria Broth (LB) shake culture containing 100 μg/ml ampicillin for selection of transformed cells.

    Article Title: A Non-Invasive Tool for Real-Time Measurement of Sulfate in Living Cells
    Article Snippet: .. In Bacteria The ECFP_sbp_mVenus chimeric construct was ligated with the pRSET-B expression vector (Invitrogen, USA) and was transformed into the BL21-CodonPlus (DE3) strain of E. coli. .. A single transformed colony was grown on LB medium for three days at 20 °C in the dark to prevent fluorescent protein from photobleaching.

    Recombinant:

    Article Title: GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28
    Article Snippet: .. The PCR product was digested and subcloned into the pRSET–C bacterial expression vector (Invitrogen) to obtain a His6 N–terminal-tagged recombinant GATE–16 protein. .. The pRSET–C plasmid containing GATE–16 was transformed into E.coli BL–21.

    Article Title: Suppression of Dendritic Cell Activation by Diabetes Autoantigens Linked to the Cholera Toxin B Subunit
    Article Snippet: .. The expression vector PRSET-CTB-INS containing the gene encoding the CTB-INS fusion protein was introduced into E. coli producer strain BL21 (DE3)pLysS (Invitrogen, Carlsbad, CA), for nickel affinity column isolation of the recombinant protein ( ). .. The CTB-INS transformed E. coli strain BL-21, was grown in 250 ml Luria Broth (LB) medium containing ampicillin (100mg/ml).

    Plasmid Preparation:

    Article Title: Induction of Indoleamine 2, 3-Dioxygenase in Human Dendritic Cells by a Cholera Toxin B Subunit—Proinsulin Vaccine
    Article Snippet: .. Expression vector pRSET-CTB-INS was transformed into the E . coli producer strain BL21 (DE3) pLysS (Invitrogen, Carlsbad, CA) for production and isolation of milligram amounts of the CTB-INS protein for further experiments [ ]. .. Synthesis and isolation of CTB-INS fusion protein The E . coli strain BL21 transformed with pRSET-CTB-INS [ ] was grown overnight at 37°C in a 2.0 ml Luria Broth (LB) shake culture containing 100 μg/ml ampicillin for selection of transformed cells.

    Article Title: GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28
    Article Snippet: .. The PCR product was digested and subcloned into the pRSET–C bacterial expression vector (Invitrogen) to obtain a His6 N–terminal-tagged recombinant GATE–16 protein. .. The pRSET–C plasmid containing GATE–16 was transformed into E.coli BL–21.

    Article Title: A Non-Invasive Tool for Real-Time Measurement of Sulfate in Living Cells
    Article Snippet: .. In Bacteria The ECFP_sbp_mVenus chimeric construct was ligated with the pRSET-B expression vector (Invitrogen, USA) and was transformed into the BL21-CodonPlus (DE3) strain of E. coli. .. A single transformed colony was grown on LB medium for three days at 20 °C in the dark to prevent fluorescent protein from photobleaching.

    Article Title: Formation of Spindle Poles by Dynein/Dynactin-Dependent Transport of Numa
    Article Snippet: .. The dynamitin PCR product was cloned in the vector pCR-TM2.1, excised using EcoR1, and cloned into the bacterial expression vector pRSET A (both from Invitrogen). .. Bacterial fusion protein was isolated using 8M urea and dialyzed against PBS before the assay.

    Article Title: Biophysical characterization and crystal structure of the Feline Immunodeficiency Virus p15 matrix protein
    Article Snippet: .. The PCR products were digested with NdeI and Bgl2 and inserted into the MCS of the pRSET-B prokaryotic expression vector (Invitrogen) digested with the same enzymes. .. This creates the pRSET-p15 expression plasmid for our p15 constructs that encode proteins with a 6-His tag at the C-terminal end of the protein.

    Article Title: Suppression of Dendritic Cell Activation by Diabetes Autoantigens Linked to the Cholera Toxin B Subunit
    Article Snippet: .. The expression vector PRSET-CTB-INS containing the gene encoding the CTB-INS fusion protein was introduced into E. coli producer strain BL21 (DE3)pLysS (Invitrogen, Carlsbad, CA), for nickel affinity column isolation of the recombinant protein ( ). .. The CTB-INS transformed E. coli strain BL-21, was grown in 250 ml Luria Broth (LB) medium containing ampicillin (100mg/ml).

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