antibodies anti rap1 mouse monoclonal  (Santa Cruz Biotechnology)

Journal: Antioxidants

Article Title: Relationships of Telomere Homeostasis with Oxidative Stress and Cardiac Dysfunction in Human Ischaemic Hearts

doi: 10.3390/antiox10111750

Figure Lengend Snippet: Dot plot graph of mRNA, miRNA and protein expression levels of main molecules associated with telomere homeostasis. ( A ) Genes of shelterin complex. ( B ) Genes involved in the maintenance shelterin complex. ( C ) CTCF and cohesin complex genes. ( D ) Genes involved in the telomere DNA repair. ( E ) miRNAs targeting TERF1 , POT1 , RAD21 and RAD51D . ( F ) Correlation of RAD51D mRNA levels with left ventricular end-systolic (LVESD) and left ventricular end-diastolic (LVEDD) diameters. ( G ) TIN2, RAP1, TANK1 and RAD51D protein levels. ( H ) Correlation of RAD51D protein levels with miR-103a-3p expression levels. ( I ) Correlation of TIN2 with TANK1 protein levels. The results were obtained by mRNA-sequencing SOLiD 5500XL platform and ncRNA-sequencing Illumina HiSeq 2500 platform. Data are presented as the mean ± SEM. au, arbitrary units. Ischemic cardiomyopathy patients (orange), controls subjects (blue). Left ventricular end-systolic diameter (black), left ventricular end-systolic diameter (grey). * p < 0.05, ** p < 0.01, *** p < 0.0001.

Article Snippet: The primary detection antibodies used were anti-Tin2 rabbit monoclonal antibody (1:500), anti-RAP1 mouse monoclonal antibody (1:500), anti-Tankyrase rabbit polyclonal antibody (1:500), anti-RAD51D rabbit monoclonal antibody (1:500), and anti-GAPDH mouse monoclonal antibody (1:500) as a loading control, all of them obtained from Abcam.

Techniques: Expressing, Sequencing

Journal: Cell reports

Article Title: Human telomerase is directly regulated by non-telomeric TRF2-G-quadruplex interaction

doi: 10.1016/j.celrep.2021.109154

Figure Lengend Snippet: (A) Scheme showing insertion of Gaussia luciferase downstream ofthe hTERT promoter (+33 to –1,267 bp) at CCR5 locus using CRISPR/Cas9-mediated editing in HEK293T cells (see Supplemental information for characterization of cells). Position of primers designed for ChIP-qPCR indicated by arrows. (B) Effect of TRF2 silencing or expression of full-length TRF2 or TRF2-deletion mutants TRF2-DelB, TRF2-DelM, and TRF2-DelB-DelM on hTERT -promoter Gaussia luciferase activity relative to untreated control cells. Normalized using total protein in each case. (C) qPCR following TRF2 ChIP at the inserted- hTERT promoter at CCR5 locus using primers shown in scheme above (A); normalized over mock (IgG). GAPDH promoter was used as negative control for TRF2 occupancy. (D and E) qPCRfollowing ChIPforTRF1, POT1, and RAP1: at the CCR5-locus-inserted- hTERT promoterand the endogenous hTERT promoter (+38to −237 bp) in HEK293T cells(D) and spanningthe endogenous hTERT promoter in HT1080cells (E). Chromosome 5p region 100 kb downstream ofthe hTERT locus reported for physical association with telomeres by looping was used as positive control and GAPDH as negative control. All error bars represent ± SDsfrom mean values; pvalues calculated by paired/unpairedttest; for(B), (D), and (E) two-wayANOVAwas used (*p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0001).

Article Snippet: RAP1 mouse monoclonal , Santa Cruz , Cat#4C8/1 SC−53434; RRID:AB_630189.

Techniques: Luciferase, CRISPR, ChIP-qPCR, Expressing, Activity Assay, Control, Negative Control, Positive Control

Journal: Cell reports

Article Title: Human telomerase is directly regulated by non-telomeric TRF2-G-quadruplex interaction

doi: 10.1016/j.celrep.2021.109154

Figure Lengend Snippet: Key Resources Table

Article Snippet: RAP1 mouse monoclonal , Santa Cruz , Cat#4C8/1 SC−53434; RRID:AB_630189.

Techniques: Immunoprecipitation, Enzyme-linked Immunosorbent Assay, Sequencing, Recombinant, Luciferase, Construct, Over Expression, Plasmid Preparation, Software, Binding Assay