antibodies against phospho p38 mapk thr180 tyr182  (Cell Signaling Technology Inc)

Journal: Frontiers in Pharmacology

Article Title: Arctiin-encapsulated DSPE-PEG bubble-like nanoparticles inhibit alveolar epithelial type 2 cell senescence to alleviate pulmonary fibrosis via the p38/p53/p21 pathway

doi: 10.3389/fphar.2023.1141800

Figure Lengend Snippet: Arctiin-encapsulated DSPE-PEG bubble-like nanoparticles (ARC@DPBNPs) attenuated A549 senescence inhibiting the p38/p53/p21 pathway. (A) Western blot showing PD169316 selectively inhibits the kinase activity of phosphorylated p38 and p38/p53/p21 pathway activation. (B) Western blot showing that ARC@DPBNPs significantly attenuated A549 senescence by downregulating p-p38 MAPK , p53, and p21 expression. (C) Immunofluorescence staining of p-p38 MAPK , p53, and p21 in A549 cells treated with PBS, ARC@DPBNPs (10 μg/mL), bleomycin (BLM) (20 μg/mL), and BLM (20 μg/mL) + ARC@DPBNPs (10 μg/mL) respectively. * p < 0.05, ** p < 0.01, *** p < 0.001; ns: not significant.

Article Snippet: Sections were incubated with rabbit antibody against p21 (10355-1-AP; 1:200 dilution; ProteinTech, Wuhan, China), rabbit antibody against P16-InkA (10883-1-AP; 1:1,000 dilution; ProteinTech), rabbit antibody against phospho-p38 MAPK (Thr180/Tyr182) (p-p38 MAPK ) (4,511; 1:800 dilution; Cell Signaling Technology, Danvers, United States), rabbit antibody against p38 MAPK polyclonal antibody (14064-1-AP, 1:100 dilution; ProteinTech); mouse antibody against p53 (60283-2-Ig, 1:1,000 dilution; ProteinTech) overnight at 4°C, followed by incubation for 1 h at 37°C in the dark with horseradish peroxidase-labeled secondary antibody (Gene Tech; Shanghai, China).

Techniques: Western Blot, Activity Assay, Activation Assay, Expressing, Immunofluorescence, Staining

Journal: Frontiers in Pharmacology

Article Title: Arctiin-encapsulated DSPE-PEG bubble-like nanoparticles inhibit alveolar epithelial type 2 cell senescence to alleviate pulmonary fibrosis via the p38/p53/p21 pathway

doi: 10.3389/fphar.2023.1141800

Figure Lengend Snippet: Arctiin-encapsulated DSPE-PEG bubble-like nanoparticles (ARC@DPBNPs) suppress AEC2 senescence by inhibiting the p38/p53/p21 pathway. (A) Western blot of p-p38MAPK, p53, and p21 expression in lung tissue of mouse models treated with phosphate-buffered saline, ARC@DPBNPs (3 mg/kg/mL), bleomycin (BLM) (5 mg/kg), and BLM (5 mg/kg) + ARC@DPBNPs (3 mg/kg/mL) respectively. (B) Immunofluorescence staining of SFTPC (an AEC2‐specific marker, green) and p21 (red) was performed to confirm that senescent markers were expressed primarily in the AECs (original magnification = ×400). * p < 0.05, ** p < 0.01, *** p < 0.001; ns: not significant.

Article Snippet: Sections were incubated with rabbit antibody against p21 (10355-1-AP; 1:200 dilution; ProteinTech, Wuhan, China), rabbit antibody against P16-InkA (10883-1-AP; 1:1,000 dilution; ProteinTech), rabbit antibody against phospho-p38 MAPK (Thr180/Tyr182) (p-p38 MAPK ) (4,511; 1:800 dilution; Cell Signaling Technology, Danvers, United States), rabbit antibody against p38 MAPK polyclonal antibody (14064-1-AP, 1:100 dilution; ProteinTech); mouse antibody against p53 (60283-2-Ig, 1:1,000 dilution; ProteinTech) overnight at 4°C, followed by incubation for 1 h at 37°C in the dark with horseradish peroxidase-labeled secondary antibody (Gene Tech; Shanghai, China).

Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Marker