Journal: PeerJ

Article Title: ET-1 receptor type B (ETBR) overexpression associated with ICAM-1 downregulation leads to inflammatory attenuation in experimental autoimmune myocarditis

doi: 10.7717/peerj.16320

Figure Lengend Snippet: The brownish-yellow particles are ETBR and ICAM-1 proteins, and the blue color is the nucleus. The more specific proteins are expressed, the darker the brownish-yellow particles are.

Article Snippet: The membranes were incubated with primary antibodies against ETBR (cat. No. ab262700, 1:1,000; Abcam, Fremont, CA, USA), ICAM-1 (cat. No. ab222736, 1:1,000; Abcam, Fremont, CA, USA), and GAPDH (cat. No. ab37168, 1:1,000; Abcam, Fremont, CA, USA) at 4 °C overnight and with a secondary antibody (cat. No. SA00001-2, 1:5,000; Proteintech, Rosemont, IL, USA) at room temperature for 1 h. The proteins on the membranes were visualized using an enhanced chemiluminescence (ECL) kit (cat. No. PK10003; Proteintech, Rosemont, IL, USA).

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