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Santa Cruz Biotechnology antibodies against connexin 43 sc 9059
At time point 5 days post seeding, primary human osteoblasts were stained for <t>connexin</t> <t>43</t> or N-cadherin (red), and nuclei (blue). Expression of connexin 43 and N-cadherin significantly increased on cell membranes of EMD coated samples. (bar = 50 µm).
Antibodies Against Connexin 43 Sc 9059, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against connexin 43 sc 9059/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
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antibodies against connexin 43 sc 9059 - by Bioz Stars, 2024-10
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1) Product Images from "Premature Osteoblast Clustering by Enamel Matrix Proteins Induces Osteoblast Differentiation through Up-Regulation of Connexin 43 and N-Cadherin"

Article Title: Premature Osteoblast Clustering by Enamel Matrix Proteins Induces Osteoblast Differentiation through Up-Regulation of Connexin 43 and N-Cadherin

Journal: PLoS ONE

doi: 10.1371/journal.pone.0023375

At time point 5 days post seeding, primary human osteoblasts were stained for connexin 43 or N-cadherin (red), and nuclei (blue). Expression of connexin 43 and N-cadherin significantly increased on cell membranes of EMD coated samples. (bar = 50 µm).
Figure Legend Snippet: At time point 5 days post seeding, primary human osteoblasts were stained for connexin 43 or N-cadherin (red), and nuclei (blue). Expression of connexin 43 and N-cadherin significantly increased on cell membranes of EMD coated samples. (bar = 50 µm).

Techniques Used: Staining, Expressing

After 2, 3, 5, 7 and 10 days post seeding, mRNA was extracted and realtime PCR was performed using specific primers for connexin 43 (A) and N-cadherin (B). When samples were pre-coated with EMD, up to 4 fold increases in gene expression were observed for connexin 43 at 2, 3 and 5 days post seeding (A). 3 fold increases in gene expression of N-cadherin were also observed (B). Additional samples were extracted for western blot analysis (C). Elevated levels of both connexin 43 and N-cadherin were observed at 2, 3, 5 and 7 days post seeding. * denotes significant difference between EMD treated sample and respective control sample (p<0.05). Data shown is the average value from 3 independent experiments (3 replicates per experiment) ± SE.
Figure Legend Snippet: After 2, 3, 5, 7 and 10 days post seeding, mRNA was extracted and realtime PCR was performed using specific primers for connexin 43 (A) and N-cadherin (B). When samples were pre-coated with EMD, up to 4 fold increases in gene expression were observed for connexin 43 at 2, 3 and 5 days post seeding (A). 3 fold increases in gene expression of N-cadherin were also observed (B). Additional samples were extracted for western blot analysis (C). Elevated levels of both connexin 43 and N-cadherin were observed at 2, 3, 5 and 7 days post seeding. * denotes significant difference between EMD treated sample and respective control sample (p<0.05). Data shown is the average value from 3 independent experiments (3 replicates per experiment) ± SE.

Techniques Used: Expressing, Western Blot



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Santa Cruz Biotechnology antibodies against connexin 43 sc 9059
At time point 5 days post seeding, primary human osteoblasts were stained for <t>connexin</t> <t>43</t> or N-cadherin (red), and nuclei (blue). Expression of connexin 43 and N-cadherin significantly increased on cell membranes of EMD coated samples. (bar = 50 µm).
Antibodies Against Connexin 43 Sc 9059, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against connexin 43 sc 9059/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
antibodies against connexin 43 sc 9059 - by Bioz Stars, 2024-10
86/100 stars
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At time point 5 days post seeding, primary human osteoblasts were stained for connexin 43 or N-cadherin (red), and nuclei (blue). Expression of connexin 43 and N-cadherin significantly increased on cell membranes of EMD coated samples. (bar = 50 µm).

Journal: PLoS ONE

Article Title: Premature Osteoblast Clustering by Enamel Matrix Proteins Induces Osteoblast Differentiation through Up-Regulation of Connexin 43 and N-Cadherin

doi: 10.1371/journal.pone.0023375

Figure Lengend Snippet: At time point 5 days post seeding, primary human osteoblasts were stained for connexin 43 or N-cadherin (red), and nuclei (blue). Expression of connexin 43 and N-cadherin significantly increased on cell membranes of EMD coated samples. (bar = 50 µm).

Article Snippet: Cells were permealized with Triton X-100 for 5 minutes, followed by staining with antibodies against connexin 43 (sc-9059) or N-cadherin (sc-7939) (Santa Cruz Biotechnologies Inc., Heidelberg, Germany) antibodies, followed by a goat anti-rabbit IgG conjugated to texas red at a dilution of 1∶200 in 0.5% PBS/bovine serum albumin (Invitrogen).

Techniques: Staining, Expressing

After 2, 3, 5, 7 and 10 days post seeding, mRNA was extracted and realtime PCR was performed using specific primers for connexin 43 (A) and N-cadherin (B). When samples were pre-coated with EMD, up to 4 fold increases in gene expression were observed for connexin 43 at 2, 3 and 5 days post seeding (A). 3 fold increases in gene expression of N-cadherin were also observed (B). Additional samples were extracted for western blot analysis (C). Elevated levels of both connexin 43 and N-cadherin were observed at 2, 3, 5 and 7 days post seeding. * denotes significant difference between EMD treated sample and respective control sample (p<0.05). Data shown is the average value from 3 independent experiments (3 replicates per experiment) ± SE.

Journal: PLoS ONE

Article Title: Premature Osteoblast Clustering by Enamel Matrix Proteins Induces Osteoblast Differentiation through Up-Regulation of Connexin 43 and N-Cadherin

doi: 10.1371/journal.pone.0023375

Figure Lengend Snippet: After 2, 3, 5, 7 and 10 days post seeding, mRNA was extracted and realtime PCR was performed using specific primers for connexin 43 (A) and N-cadherin (B). When samples were pre-coated with EMD, up to 4 fold increases in gene expression were observed for connexin 43 at 2, 3 and 5 days post seeding (A). 3 fold increases in gene expression of N-cadherin were also observed (B). Additional samples were extracted for western blot analysis (C). Elevated levels of both connexin 43 and N-cadherin were observed at 2, 3, 5 and 7 days post seeding. * denotes significant difference between EMD treated sample and respective control sample (p<0.05). Data shown is the average value from 3 independent experiments (3 replicates per experiment) ± SE.

Article Snippet: Cells were permealized with Triton X-100 for 5 minutes, followed by staining with antibodies against connexin 43 (sc-9059) or N-cadherin (sc-7939) (Santa Cruz Biotechnologies Inc., Heidelberg, Germany) antibodies, followed by a goat anti-rabbit IgG conjugated to texas red at a dilution of 1∶200 in 0.5% PBS/bovine serum albumin (Invitrogen).

Techniques: Expressing, Western Blot