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p ampk  (Cell Signaling Technology Inc)


Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc p ampk
    Primary and secondary antibodies used in this study
    P Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p ampk/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p ampk - by Bioz Stars, 2025-01
    86/100 stars

    Images

    1) Product Images from "Prolonged intermittent theta burst stimulation restores the balance between A 2A R- and A 1 R-mediated adenosine signaling in the 6-hydroxidopamine model of Parkinson’s disease"

    Article Title: Prolonged intermittent theta burst stimulation restores the balance between A 2A R- and A 1 R-mediated adenosine signaling in the 6-hydroxidopamine model of Parkinson’s disease

    Journal: Neural Regeneration Research

    doi: 10.4103/NRR.NRR-D-23-01542

    Primary and secondary antibodies used in this study
    Figure Legend Snippet: Primary and secondary antibodies used in this study

    Techniques Used: Western Blot, Immunohistochemistry

    Effects of iTBS on cytosolic pools of adenosine-generating and adenosine-eliminating enzymes and AMP kinase in crude synaptosomal fraction of the caudoputamen of 6-OHDA-lesioned rats. (A) Representative support membranes showing density of 55-kDa (eN/CD73) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing eN/CD73 protein abundance in cytosolic fraction of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. (B) Representative support membranes showing density of 42-kDa (ADA2) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing ADA1 protein abundance in cytosolic fraction of the CPu of sham-stimulated and iTBS-stimulated animals 1 and 3 weeks after stimulation. (C) Representative support membranes showing density of 60-kDa (phospho-AMPK) and 60-kDa (total-AMPK) bands and histograms of immunoblot analysis showing ratio of p-AMPK and t-AMPK protein abundance in cytosolic fraction fractions of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. All data are represented as mean ± SD. Dots in the graphs represent individual values. The number at the bottom of the graphs represents the number of individual animals included in analysis. All data are expressed as the percentage (%) of contralateral (left) CPu (dotted line at 100%). (D) The AMP phosphohydrolase/cN-II activity was assayed in the cytosolic fraction of the CPu from both sham-stimulated and iTBS-stimulated animals, at both 1 and 3 weeks after the stimulation. The bars depicted represent the mean activity (expressed as nmol Pi/mg/min) ± SEM, based on five determinations performed in duplicate. (E) The AMP phosphohydrolase activity was measured in both the absence and presence of the specific eN/CD73 inhibitor MRS 4592. The bars represent the mean activity (expressed as nmol Pi/mg/min) ± SEM, calculated from five determinations performed in duplicate. The numbers at the bottom of the graphs indicate the total number of individual animals included in the analysis. Significance shown inside graphs: * P < 0.05, *** P < 0.001, **** P < 0.0001 (unpaired Student’s t -test). All experiments are repeated at least twice. 6-OHDA: 6-Hydroxydopamine; CPu: caudoputamen; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; iTBS: intermittent theta burst stimulation; ns: not significant.
    Figure Legend Snippet: Effects of iTBS on cytosolic pools of adenosine-generating and adenosine-eliminating enzymes and AMP kinase in crude synaptosomal fraction of the caudoputamen of 6-OHDA-lesioned rats. (A) Representative support membranes showing density of 55-kDa (eN/CD73) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing eN/CD73 protein abundance in cytosolic fraction of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. (B) Representative support membranes showing density of 42-kDa (ADA2) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing ADA1 protein abundance in cytosolic fraction of the CPu of sham-stimulated and iTBS-stimulated animals 1 and 3 weeks after stimulation. (C) Representative support membranes showing density of 60-kDa (phospho-AMPK) and 60-kDa (total-AMPK) bands and histograms of immunoblot analysis showing ratio of p-AMPK and t-AMPK protein abundance in cytosolic fraction fractions of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. All data are represented as mean ± SD. Dots in the graphs represent individual values. The number at the bottom of the graphs represents the number of individual animals included in analysis. All data are expressed as the percentage (%) of contralateral (left) CPu (dotted line at 100%). (D) The AMP phosphohydrolase/cN-II activity was assayed in the cytosolic fraction of the CPu from both sham-stimulated and iTBS-stimulated animals, at both 1 and 3 weeks after the stimulation. The bars depicted represent the mean activity (expressed as nmol Pi/mg/min) ± SEM, based on five determinations performed in duplicate. (E) The AMP phosphohydrolase activity was measured in both the absence and presence of the specific eN/CD73 inhibitor MRS 4592. The bars represent the mean activity (expressed as nmol Pi/mg/min) ± SEM, calculated from five determinations performed in duplicate. The numbers at the bottom of the graphs indicate the total number of individual animals included in the analysis. Significance shown inside graphs: * P < 0.05, *** P < 0.001, **** P < 0.0001 (unpaired Student’s t -test). All experiments are repeated at least twice. 6-OHDA: 6-Hydroxydopamine; CPu: caudoputamen; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; iTBS: intermittent theta burst stimulation; ns: not significant.

    Techniques Used: Western Blot, Activity Assay



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    Image Search Results


    Primary and secondary antibodies used in this study

    Journal: Neural Regeneration Research

    Article Title: Prolonged intermittent theta burst stimulation restores the balance between A 2A R- and A 1 R-mediated adenosine signaling in the 6-hydroxidopamine model of Parkinson’s disease

    doi: 10.4103/NRR.NRR-D-23-01542

    Figure Lengend Snippet: Primary and secondary antibodies used in this study

    Article Snippet: p-AMPK , Rabbit, polyclonal , 1:2000 WB , Cell Signaling Technology , 2535 , AB_331250.

    Techniques: Western Blot, Immunohistochemistry

    Effects of iTBS on cytosolic pools of adenosine-generating and adenosine-eliminating enzymes and AMP kinase in crude synaptosomal fraction of the caudoputamen of 6-OHDA-lesioned rats. (A) Representative support membranes showing density of 55-kDa (eN/CD73) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing eN/CD73 protein abundance in cytosolic fraction of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. (B) Representative support membranes showing density of 42-kDa (ADA2) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing ADA1 protein abundance in cytosolic fraction of the CPu of sham-stimulated and iTBS-stimulated animals 1 and 3 weeks after stimulation. (C) Representative support membranes showing density of 60-kDa (phospho-AMPK) and 60-kDa (total-AMPK) bands and histograms of immunoblot analysis showing ratio of p-AMPK and t-AMPK protein abundance in cytosolic fraction fractions of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. All data are represented as mean ± SD. Dots in the graphs represent individual values. The number at the bottom of the graphs represents the number of individual animals included in analysis. All data are expressed as the percentage (%) of contralateral (left) CPu (dotted line at 100%). (D) The AMP phosphohydrolase/cN-II activity was assayed in the cytosolic fraction of the CPu from both sham-stimulated and iTBS-stimulated animals, at both 1 and 3 weeks after the stimulation. The bars depicted represent the mean activity (expressed as nmol Pi/mg/min) ± SEM, based on five determinations performed in duplicate. (E) The AMP phosphohydrolase activity was measured in both the absence and presence of the specific eN/CD73 inhibitor MRS 4592. The bars represent the mean activity (expressed as nmol Pi/mg/min) ± SEM, calculated from five determinations performed in duplicate. The numbers at the bottom of the graphs indicate the total number of individual animals included in the analysis. Significance shown inside graphs: * P < 0.05, *** P < 0.001, **** P < 0.0001 (unpaired Student’s t -test). All experiments are repeated at least twice. 6-OHDA: 6-Hydroxydopamine; CPu: caudoputamen; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; iTBS: intermittent theta burst stimulation; ns: not significant.

    Journal: Neural Regeneration Research

    Article Title: Prolonged intermittent theta burst stimulation restores the balance between A 2A R- and A 1 R-mediated adenosine signaling in the 6-hydroxidopamine model of Parkinson’s disease

    doi: 10.4103/NRR.NRR-D-23-01542

    Figure Lengend Snippet: Effects of iTBS on cytosolic pools of adenosine-generating and adenosine-eliminating enzymes and AMP kinase in crude synaptosomal fraction of the caudoputamen of 6-OHDA-lesioned rats. (A) Representative support membranes showing density of 55-kDa (eN/CD73) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing eN/CD73 protein abundance in cytosolic fraction of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. (B) Representative support membranes showing density of 42-kDa (ADA2) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing ADA1 protein abundance in cytosolic fraction of the CPu of sham-stimulated and iTBS-stimulated animals 1 and 3 weeks after stimulation. (C) Representative support membranes showing density of 60-kDa (phospho-AMPK) and 60-kDa (total-AMPK) bands and histograms of immunoblot analysis showing ratio of p-AMPK and t-AMPK protein abundance in cytosolic fraction fractions of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. All data are represented as mean ± SD. Dots in the graphs represent individual values. The number at the bottom of the graphs represents the number of individual animals included in analysis. All data are expressed as the percentage (%) of contralateral (left) CPu (dotted line at 100%). (D) The AMP phosphohydrolase/cN-II activity was assayed in the cytosolic fraction of the CPu from both sham-stimulated and iTBS-stimulated animals, at both 1 and 3 weeks after the stimulation. The bars depicted represent the mean activity (expressed as nmol Pi/mg/min) ± SEM, based on five determinations performed in duplicate. (E) The AMP phosphohydrolase activity was measured in both the absence and presence of the specific eN/CD73 inhibitor MRS 4592. The bars represent the mean activity (expressed as nmol Pi/mg/min) ± SEM, calculated from five determinations performed in duplicate. The numbers at the bottom of the graphs indicate the total number of individual animals included in the analysis. Significance shown inside graphs: * P < 0.05, *** P < 0.001, **** P < 0.0001 (unpaired Student’s t -test). All experiments are repeated at least twice. 6-OHDA: 6-Hydroxydopamine; CPu: caudoputamen; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; iTBS: intermittent theta burst stimulation; ns: not significant.

    Article Snippet: p-AMPK , Rabbit, polyclonal , 1:2000 WB , Cell Signaling Technology , 2535 , AB_331250.

    Techniques: Western Blot, Activity Assay