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anti-neurod1 rabbit polyclonal antibody (CeMines Inc)

Name: anti-neurod1 rabbit polyclonal antibody
Brand: CeMines Inc
Rating: 90 (1 reviews)

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CeMines Inc anti-neurod1 rabbit polyclonal antibody

A, dose-dependent activation of the IA-1 promoter by <t>NeuroD1</t> in HeLa cells. Different concentrations of CMV-NeuroD1 constructs were co-transfected with the IA-1 promoter-CAT construct into HeLa cells. Dosage-dependent activation of the IA-1 promoter by the CMV-NeuroD1 cDNA expression vector is shown as -fold increase. The CAT activities were normalized by protein concentrations. The transfections were repeated three times. B, co-transfection of E47 and/or NeuroD1 enhanced the IA-1 promoter activity in HeLa cells. E47 and NeuroD1 together demonstrated much higher promoter activity than each transcription factor alone, suggesting heterodimer activation of the IA-1 promoter. The CMV-β-gal vector was used as internal control to normalize transfection efficiency. The transfections were repeated three times.

Anti Neurod1 Rabbit Polyclonal Antibody, supplied by CeMines Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-neurod1 rabbit polyclonal antibody/product/CeMines Inc
Average 90 stars, based on 1 article reviews

anti-neurod1 rabbit polyclonal antibody - by Bioz Stars, 2026-03

90/100 stars

Images

1) Product Images from "NeuroD1/E47 Regulates the E-box Element of a Novel Zinc Finger Transcription Factor, IA-1, in Developing Nervous System * "

Article Title: NeuroD1/E47 Regulates the E-box Element of a Novel Zinc Finger Transcription Factor, IA-1, in Developing Nervous System *

Journal:

doi: 10.1074/jbc.M306795200

A, dose-dependent activation of the IA-1 promoter by NeuroD1 in HeLa cells. Different concentrations of CMV-NeuroD1 constructs were co-transfected with the IA-1 promoter-CAT construct into HeLa cells. Dosage-dependent activation of the IA-1 promoter by the CMV-NeuroD1 cDNA expression vector is shown as -fold increase. The CAT activities were normalized by protein concentrations. The transfections were repeated three times. B, co-transfection of E47 and/or NeuroD1 enhanced the IA-1 promoter activity in HeLa cells. E47 and NeuroD1 together demonstrated much higher promoter activity than each transcription factor alone, suggesting heterodimer activation of the IA-1 promoter. The CMV-β-gal vector was used as internal control to normalize transfection efficiency. The transfections were repeated three times.

Figure Legend Snippet: A, dose-dependent activation of the IA-1 promoter by NeuroD1 in HeLa cells. Different concentrations of CMV-NeuroD1 constructs were co-transfected with the IA-1 promoter-CAT construct into HeLa cells. Dosage-dependent activation of the IA-1 promoter by the CMV-NeuroD1 cDNA expression vector is shown as -fold increase. The CAT activities were normalized by protein concentrations. The transfections were repeated three times. B, co-transfection of E47 and/or NeuroD1 enhanced the IA-1 promoter activity in HeLa cells. E47 and NeuroD1 together demonstrated much higher promoter activity than each transcription factor alone, suggesting heterodimer activation of the IA-1 promoter. The CMV-β-gal vector was used as internal control to normalize transfection efficiency. The transfections were repeated three times.

Techniques Used: Activation Assay, Construct, Transfection, Expressing, Plasmid Preparation, Cotransfection, Activity Assay

Three copies of each E-box (E1, E2, and E3) were cloned into E1bTATA-CAT vector. Each reporter vector was co-transfected with pcDNA, NeuroD1, E47, or E47-NeuroD1 into HeLa cells. The E47 homodimer and the E47-NeuroD1 heterodimer showed a strong activation of the E3-box construct. The CMV-β-gal vector was used as internal control to normalize transfection efficiency. The transfections were repeated three times.

Figure Legend Snippet: Three copies of each E-box (E1, E2, and E3) were cloned into E1bTATA-CAT vector. Each reporter vector was co-transfected with pcDNA, NeuroD1, E47, or E47-NeuroD1 into HeLa cells. The E47 homodimer and the E47-NeuroD1 heterodimer showed a strong activation of the E3-box construct. The CMV-β-gal vector was used as internal control to normalize transfection efficiency. The transfections were repeated three times.

Techniques Used: Clone Assay, Plasmid Preparation, Transfection, Activation Assay, Construct

A, the NeuroD1/E47 heterodimer and the E47 homodimer can specifically bind to the E3-box. The radiolabeled IA-1 promoter (−192/−165 bp) containing the E3-box was incubated with E47 or E47-NeuroD1 with or without antibodies to E47 or NeuroD1. Both the homodimer and the heterodimer were shown in shifted bands. E47 antibody supershifted the homodimer, whereas the NeuroD1 antibody interfered with the heterodimer binding. NS, nonspecific band. B, competitive inhibition of the E47-NeuroD1 complex binding with 3× E3-box. Incubation of E47 and NeuroD1 proteins with labeled 3× E3 oligonucleotide in the presence or absence of competitor (50- or 100-fold molar excess) showed that E47-NeuroD1 binding is specific for the E3-box alone.

Figure Legend Snippet: A, the NeuroD1/E47 heterodimer and the E47 homodimer can specifically bind to the E3-box. The radiolabeled IA-1 promoter (−192/−165 bp) containing the E3-box was incubated with E47 or E47-NeuroD1 with or without antibodies to E47 or NeuroD1. Both the homodimer and the heterodimer were shown in shifted bands. E47 antibody supershifted the homodimer, whereas the NeuroD1 antibody interfered with the heterodimer binding. NS, nonspecific band. B, competitive inhibition of the E47-NeuroD1 complex binding with 3× E3-box. Incubation of E47 and NeuroD1 proteins with labeled 3× E3 oligonucleotide in the presence or absence of competitor (50- or 100-fold molar excess) showed that E47-NeuroD1 binding is specific for the E3-box alone.

Techniques Used: Incubation, Binding Assay, Inhibition, Labeling

Twenty micrograms of total RNA from Daoy, D283MED, U87MG, WERI-Rb-1, Y79, and βTC-1 cell lines were resolved on a 1% agarose/formaldehyde gel and transferred to a nitrocellulose membrane. The samples were run as two separate sets on the same gel. The membrane was divided in half, and the left half was hybridized with a mixture of human and mouse IA-1 probes (A) and the right half was hybridized with a hamster NeuroD1/β2 probe (B). The ethidium bromide-stained gel was shown below the blot for comparison of loading differences. The IA-1 gene expression in the neuroendocrine cell lines overlaps with the NeuroD1 message completely.

Figure Legend Snippet: Twenty micrograms of total RNA from Daoy, D283MED, U87MG, WERI-Rb-1, Y79, and βTC-1 cell lines were resolved on a 1% agarose/formaldehyde gel and transferred to a nitrocellulose membrane. The samples were run as two separate sets on the same gel. The membrane was divided in half, and the left half was hybridized with a mixture of human and mouse IA-1 probes (A) and the right half was hybridized with a hamster NeuroD1/β2 probe (B). The ethidium bromide-stained gel was shown below the blot for comparison of loading differences. The IA-1 gene expression in the neuroendocrine cell lines overlaps with the NeuroD1 message completely.

Techniques Used: Staining, Expressing

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Journal:

Article Title: NeuroD1/E47 Regulates the E-box Element of a Novel Zinc Finger Transcription Factor, IA-1, in Developing Nervous System *

doi: 10.1074/jbc.M306795200

Figure Lengend Snippet: A, dose-dependent activation of the IA-1 promoter by NeuroD1 in HeLa cells. Different concentrations of CMV-NeuroD1 constructs were co-transfected with the IA-1 promoter-CAT construct into HeLa cells. Dosage-dependent activation of the IA-1 promoter by the CMV-NeuroD1 cDNA expression vector is shown as -fold increase. The CAT activities were normalized by protein concentrations. The transfections were repeated three times. B, co-transfection of E47 and/or NeuroD1 enhanced the IA-1 promoter activity in HeLa cells. E47 and NeuroD1 together demonstrated much higher promoter activity than each transcription factor alone, suggesting heterodimer activation of the IA-1 promoter. The CMV-β-gal vector was used as internal control to normalize transfection efficiency. The transfections were repeated three times.

Article Snippet: Synthesis of E47 and NeuroD1 was confirmed by Western blot analysis using an anti-E47 rabbit polyclonal antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) or an anti-NeuroD1 rabbit polyclonal antibody (CeMines).

Techniques: Activation Assay, Construct, Transfection, Expressing, Plasmid Preparation, Cotransfection, Activity Assay

Journal:

Article Title: NeuroD1/E47 Regulates the E-box Element of a Novel Zinc Finger Transcription Factor, IA-1, in Developing Nervous System *

doi: 10.1074/jbc.M306795200

Figure Lengend Snippet: Three copies of each E-box (E1, E2, and E3) were cloned into E1bTATA-CAT vector. Each reporter vector was co-transfected with pcDNA, NeuroD1, E47, or E47-NeuroD1 into HeLa cells. The E47 homodimer and the E47-NeuroD1 heterodimer showed a strong activation of the E3-box construct. The CMV-β-gal vector was used as internal control to normalize transfection efficiency. The transfections were repeated three times.

Techniques: Clone Assay, Plasmid Preparation, Transfection, Activation Assay, Construct

Journal:

Article Title: NeuroD1/E47 Regulates the E-box Element of a Novel Zinc Finger Transcription Factor, IA-1, in Developing Nervous System *

doi: 10.1074/jbc.M306795200

Figure Lengend Snippet: A, the NeuroD1/E47 heterodimer and the E47 homodimer can specifically bind to the E3-box. The radiolabeled IA-1 promoter (−192/−165 bp) containing the E3-box was incubated with E47 or E47-NeuroD1 with or without antibodies to E47 or NeuroD1. Both the homodimer and the heterodimer were shown in shifted bands. E47 antibody supershifted the homodimer, whereas the NeuroD1 antibody interfered with the heterodimer binding. NS, nonspecific band. B, competitive inhibition of the E47-NeuroD1 complex binding with 3× E3-box. Incubation of E47 and NeuroD1 proteins with labeled 3× E3 oligonucleotide in the presence or absence of competitor (50- or 100-fold molar excess) showed that E47-NeuroD1 binding is specific for the E3-box alone.

Techniques: Incubation, Binding Assay, Inhibition, Labeling

Journal:

Article Title: NeuroD1/E47 Regulates the E-box Element of a Novel Zinc Finger Transcription Factor, IA-1, in Developing Nervous System *

doi: 10.1074/jbc.M306795200

Figure Lengend Snippet: Twenty micrograms of total RNA from Daoy, D283MED, U87MG, WERI-Rb-1, Y79, and βTC-1 cell lines were resolved on a 1% agarose/formaldehyde gel and transferred to a nitrocellulose membrane. The samples were run as two separate sets on the same gel. The membrane was divided in half, and the left half was hybridized with a mixture of human and mouse IA-1 probes (A) and the right half was hybridized with a hamster NeuroD1/β2 probe (B). The ethidium bromide-stained gel was shown below the blot for comparison of loading differences. The IA-1 gene expression in the neuroendocrine cell lines overlaps with the NeuroD1 message completely.

Techniques: Staining, Expressing

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Images

1) Product Images from "NeuroD1/E47 Regulates the E-box Element of a Novel Zinc Finger Transcription Factor, IA-1, in Developing Nervous System * "

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