Journal: bioRxiv
Article Title: Generation of Self-Organising Macrovascular Constructs by Bioprinting human iPSC-Derived Mesodermal Progenitor Cells
doi: 10.64898/2026.03.16.712040
Figure Lengend Snippet: (A1) and (B1) Phase contrast images of hiPSCs and HiMPCs. (A1) hiPSCs and (B1) hiMPCs cultured in standard 2D conditions. (A2 & A3) Immunofluorescence images showing hiPCS stained for SOX2 and OCT4 (both in yellow). (B2 & B3) hiMPCs stained for VEGFR-2 and BryT (both in red). (C1) Schematic representation of the in-gel printing-embedded bioprinting-approach. (C2) Printed and crosslinked FGXC tube. (C3) Maximum intensity projection of z-stack images from a cleared printed tube at day 12. The endothelization around the artificial lumen and endothelial network within the tube wall is shown by CD31 + staining (red). αSMA + cells are shown in green.
Article Snippet: The following antibodies were used: OCT4 (sc-5279, Santa Cruz Biotech, Dallas, TX, USA), SOX2 (MAB2018, Santa Cruz Biotech), Brachyury/T (AF2085, Santa Cruz Biotech) and VEGFR-2 (sc-48161, Santa Cruz Biotech).
Techniques: Cell Culture, Immunofluorescence, Staining