terf2  (Proteintech)

Journal: Scientific Reports

Article Title: The role of cellular senescence-related genes in ischemia–reperfusion injury and the identification of their biomarkers

doi: 10.1038/s41598-026-36076-2

Figure Lengend Snippet: Internal validation of combined IRI diagnostic model. ( a ) Nomogram of Model Genes in the diagnostic model of IRI in the combined GEO dataset. ( b ) DCA plot of the Model Genes in the Combined GEO Dataset for the Diagnostic Model of IRI. ( c ) ROC curve of the RiskScore of the IRI diagnostic model from the combined GEO dataset. D-I. ROC curves for CDKN2B ( d ), ID1 ( e ), STAT3 ( f ), TERF2 ( g ), TP53 ( h ), and ZNF277 ( i ) were analyzed between disease control groups using the combined GEO dataset. AUC < 0.9 indicated high accuracy, 0.7 < AUC0.9 indicated substantial accuracy, and 0.5 < AUC < 0.7 indicated lower accuracy.

Article Snippet: The slides were incubated with primary antibodies: anti-CDKN2B (1:50, Biodragon, China), TP53 (1:50, ProteinTech Group, China), TERF2 (1:50, ProteinTech Group, China), STAT3 (1:600, Servicebio, China), and ID1 (1:800, Servicebio, China).

Techniques: Biomarker Discovery, Diagnostic Assay, Control

Journal: Scientific Reports

Article Title: The role of cellular senescence-related genes in ischemia–reperfusion injury and the identification of their biomarkers

doi: 10.1038/s41598-026-36076-2

Figure Lengend Snippet: In vivo verification of targeted genes in IRI mice. ( a ) Workflow of experimental design. ( b - g ) mRNA levels of CDKN2B , ID1 , STAT3 , TP53 , TERF2 , and ZNF277 were assessed at different time points in mice model of IRI. ( h ) IHC of 5 model genes with AUC > 0.9. Bar = 50 μm. ( i - m ) Statistical plot of average optical density values of IHC for model genes ( CDKN2B , TP53 , TERF2 , STAT3 , and ID1 ). * p < 0.05, **p < 0.01, and *** p < 0.001.

Article Snippet: The slides were incubated with primary antibodies: anti-CDKN2B (1:50, Biodragon, China), TP53 (1:50, ProteinTech Group, China), TERF2 (1:50, ProteinTech Group, China), STAT3 (1:600, Servicebio, China), and ID1 (1:800, Servicebio, China).

Techniques: In Vivo

Journal: Cell reports

Article Title: Topoisomerase IIIα controls alternative lengthening of telomeres

doi: 10.1016/j.celrep.2025.116066

Figure Lengend Snippet: (A) Representative immunofluorescence images showing colocalization of HALO-TOP3A with GFP-TERF2 in U2OS. The scale bar represents 10 μm. (B) Representative immunofluorescence images showing localization of TOP3A with TERF2 in U2OS (ALT). (C) Quantification for TOP3A-TERF2 co-foci per nucleus in U2OS. 100 nuclei were calculated. (D) Representative immunofluorescence images showing localization of TOP3A with TERF2 in U2OS (ALT) and SJSA1 (Tel) cells. The scale bar represents 20 μm. (E) Quantification for TOP3A-TERF2 co-foci per nucleus in U2OS and SJSA1 cells. 25 nuclei per experiment were calculated and compared ( n = 2). (F) Proximity ligation assay (PLA) showing interaction of TOP3A with TERF2 and PML in U2OS cells. The scale bar represents 10 μm.

Article Snippet: Primary antibodies for TERF2 (1:1000 dilution, NB110–57130, Novus Biologicals, rabbit and 1:250 dilution,4A794, Millipore sigma, mouse), PML (1:250 dilution, sc-966, Santa Cruz Biotechnology, mouse) and BLM (1:250 dilution, A-300–110, Bethyl laboratories, Rabbit) in the blocking buffer were incubated for 1 h at room temperature.

Techniques: Immunofluorescence, Proximity Ligation Assay

Journal: Cell reports

Article Title: Topoisomerase IIIα controls alternative lengthening of telomeres

doi: 10.1016/j.celrep.2025.116066

Figure Lengend Snippet: (A) Representative immunofluorescence images showing localization of PML and TERF2 in U2OS cells after siTOP3A treatment. The scale bar represents 10 μm. (B) Quantification for TERF2-PML co-foci per nucleus. 40 nuclei were counted for each condition ( n = 3). Data are plotted as means ± SDs. **** p ≤ 0.0001 (two-tailed unpaired t test). (C) Representative immunofluorescence images showing localization of BLM and TERF2 in U2OS cells after siTOP3A treatment. The scale bar represents 10 μm. (D) Quantification for TERF2-BLM co-foci per nucleus. 40 nuclei were counted for each condition ( n = 3). Error bars represent means ± SDs. **** p ≤ 0.0001 (two-tailed unpaired t test). (E) Schematic representation of ssTeloC detection (telomere probe under native conditions). (F) Representative images of ssTeloC staining performed after native FISH (scale bar: 10 μm). (G) Quantification for ssTeloC foci per nucleus in U2OS. 50 nuclei were counted for each condition ( n = 3). Data are plotted as means ± SDs. **** p ≤ 0.0001 (two-tailed unpaired t test).

Article Snippet: Primary antibodies for TERF2 (1:1000 dilution, NB110–57130, Novus Biologicals, rabbit and 1:250 dilution,4A794, Millipore sigma, mouse), PML (1:250 dilution, sc-966, Santa Cruz Biotechnology, mouse) and BLM (1:250 dilution, A-300–110, Bethyl laboratories, Rabbit) in the blocking buffer were incubated for 1 h at room temperature.

Techniques: Immunofluorescence, Two Tailed Test, Staining

Journal: Cell reports

Article Title: Topoisomerase IIIα controls alternative lengthening of telomeres

doi: 10.1016/j.celrep.2025.116066

Figure Lengend Snippet: (A) Scheme of TERRA R-loops. (B) Representative images of RNA FISH for TERRA in U2OS cells after TOP3A knockdown (scale bar: 10 μm). (C) Quantification of TERRA foci per nucleus. 50 nuclei were counted for each condition ( n = 3). Error bars represent means ± SDs. **** p ≤ 0.0001 (two-tailed unpaired t test). (D) Quantification for TERF2 foci per nucleus in U2OS. 40 nuclei were counted for each condition ( n = 3). Data are plotted as means ± SDs. **** p ≤ 0.0001 (two-tailed unpaired t test).

Article Snippet: Primary antibodies for TERF2 (1:1000 dilution, NB110–57130, Novus Biologicals, rabbit and 1:250 dilution,4A794, Millipore sigma, mouse), PML (1:250 dilution, sc-966, Santa Cruz Biotechnology, mouse) and BLM (1:250 dilution, A-300–110, Bethyl laboratories, Rabbit) in the blocking buffer were incubated for 1 h at room temperature.

Techniques: Knockdown, Two Tailed Test

Journal: Cell reports

Article Title: Topoisomerase IIIα controls alternative lengthening of telomeres

doi: 10.1016/j.celrep.2025.116066

Figure Lengend Snippet: (A) Fluorescence-activated cell sorting (FACS) analysis of HT1080 cells transfected with siTOP3A and harvested after 72 h. EdU (10 μM) was added during the final 30 min before cell harvesting. EdU/DAPI uptake per cell was analyzed. (B) FACS analysis of U2OS cells transfected with siTOP3A and harvested after 72 h. EdU (10 μM) was added during the final 30 min before cell harvesting. EdU/DAPI uptake per cell was analyzed. (C) Representative western blots after TOP3A knockdown in U2OS and HT1080 cells. (D) Quantification of TERF2 intensity using ImageJ software and normalization with GAPDH controls. The experiment was performed in triplicate and the average TERF2 intensity calculated. Error bars represent means ± SDs. n.s., not significant and *** p ≤ 0.0008 (two-tailed unpaired t test). (E) Cycloheximide chase experiment monitoring TERF2 stability after siRNA-mediated TOP3A knockdown for 48 h in U2OS cells at the indicated time intervals. (F) Quantification of TERF2 intensity in U2OS using ImageJ software and normalization with GAPDH. (G) Cycloheximide chase experiment monitoring TERF2 stability after siRNA-mediated TOP3A knockdown for 72 h in HT1080 cells at the indicated time intervals. (H) Quantification of TERF2 intensity in HT1080 using ImageJ software and normalization with PCNA.

Article Snippet: Primary antibodies for TERF2 (1:1000 dilution, NB110–57130, Novus Biologicals, rabbit and 1:250 dilution,4A794, Millipore sigma, mouse), PML (1:250 dilution, sc-966, Santa Cruz Biotechnology, mouse) and BLM (1:250 dilution, A-300–110, Bethyl laboratories, Rabbit) in the blocking buffer were incubated for 1 h at room temperature.

Techniques: Fluorescence, FACS, Transfection, Cell Harvesting, Western Blot, Knockdown, Software, Two Tailed Test

Journal: Cell reports

Article Title: Topoisomerase IIIα controls alternative lengthening of telomeres

doi: 10.1016/j.celrep.2025.116066

Figure Lengend Snippet: (A) Domain organization of human TOP3A. The arrow indicates the catalytic tyrosine and the adjacent arginine residue. (B) Representative images of RNA FISH for TERRA in U2OS cells after overexpression of WT TOP3A and TOP3A R364W. Scale bar: 20 μm. (C) Quantification for TERRA foci per nucleus in U2OS cells after overexpression of WT TOP3A and TOP3A R364W. In total, 50 nuclei were calculated ( n = 2). Data are plotted as means ± SDs. **** p ≤ 0.0001 (two-tailed unpaired t test). (D) Representative TERF2 immunoblot after overexpression of WT TOP3A and TOP3A R364W.

Article Snippet: Primary antibodies for TERF2 (1:1000 dilution, NB110–57130, Novus Biologicals, rabbit and 1:250 dilution,4A794, Millipore sigma, mouse), PML (1:250 dilution, sc-966, Santa Cruz Biotechnology, mouse) and BLM (1:250 dilution, A-300–110, Bethyl laboratories, Rabbit) in the blocking buffer were incubated for 1 h at room temperature.

Techniques: Residue, Over Expression, Two Tailed Test, Western Blot

Journal: Cell reports

Article Title: Topoisomerase IIIα controls alternative lengthening of telomeres

doi: 10.1016/j.celrep.2025.116066

Figure Lengend Snippet: (A) TOP3A interacts with the components of the BTRR complex and TERF2 and protects the shelterin complex from destabilization in ALT cells. (B) TOP3A promotes the formation of TERRA R-loops and c-circles at the telomeres of ALT cells. (C) TOP3A resolves topological DNA structures associated with D-loops during ALT synthesis (steps 1–2); TOP3A can also resolve double Holliday junctions [DHJs] to release extended telomeres without crossover [steps 5–6]) (see for details).

Article Snippet: Primary antibodies for TERF2 (1:1000 dilution, NB110–57130, Novus Biologicals, rabbit and 1:250 dilution,4A794, Millipore sigma, mouse), PML (1:250 dilution, sc-966, Santa Cruz Biotechnology, mouse) and BLM (1:250 dilution, A-300–110, Bethyl laboratories, Rabbit) in the blocking buffer were incubated for 1 h at room temperature.

Techniques: