Journal: International Journal of Molecular Sciences

Article Title: Identification of PTPN12 Phosphatase as a Novel Negative Regulator of Hippo Pathway Effectors YAP/TAZ in Breast Cancer

doi: 10.3390/ijms25074064

Figure Lengend Snippet: Parallel screens for PTPs regulating the Hippo pathway by the LATS-BS and STBS-Luc (YAP/TAZ reporter). The ratio of the LATS-BS ( A ) and STBS-Luc reporter ( B ) signals after cotransfection with phosphatases to that of LATS-BS or STBS-Luc alone was calculated based on fold changes. A fold change greater than 2 was considered significant and indicated as “*”.

Article Snippet: Primary , YAP/TAZ , YAP/TAZ Rabbit mAb , 1:1000 , Cell Signaling.

Techniques: Cotransfection

Journal: International Journal of Molecular Sciences

Article Title: Identification of PTPN12 Phosphatase as a Novel Negative Regulator of Hippo Pathway Effectors YAP/TAZ in Breast Cancer

doi: 10.3390/ijms25074064

Figure Lengend Snippet: PTPN12 regulates cell proliferation, which is mediated by the Hippo signaling pathway. ( A ) Western blot analysis of YAP and TAZ after the siYAP/siTAZ transfection of short interference RNAs targeting YAP/TAZ (siYAP/siTAZ) into MCF10A-gPTPN12 cells. ( B ) Cell proliferation assay. Cell proliferation was measured in MCF10A-PTPN12-WT (−Dox, −siYAP/siTAZ), MCF10A-PTPN12-KO (+Dox, −siYAP/siTAZ), and MCF10A-PTPN12-KO-siYAP/siTAZ (+Dox, +siYAP/siTAZ) cells. Cell numbers were counted on days 2, 4, and 6 after seeding.

Article Snippet: Primary , YAP/TAZ , YAP/TAZ Rabbit mAb , 1:1000 , Cell Signaling.

Techniques: Western Blot, Transfection, Proliferation Assay

Journal: International Journal of Molecular Sciences

Article Title: Identification of PTPN12 Phosphatase as a Novel Negative Regulator of Hippo Pathway Effectors YAP/TAZ in Breast Cancer

doi: 10.3390/ijms25074064

Figure Lengend Snippet: The overexpression of PTPN12 suppresses YAP/TAZ-induced increased mammary cell proliferation. ( A ) Inducible expression of PTPN in MCF10A-YAP or -TAZ cells. Dox was added to induce PTPN12 in MCF10A-YAP-PTPN12/pTRIPZ or MCF10A-TAZ-PTPN12/pTRIPZ cells. MCF10A cells expressing a WPI lentiviral vector expressing GFP were used as a control. Forty-eight hours after Dox induction, proteins were extracted from cells and subjected to Western blot analysis of PTPN12, YAP, and TAZ. β-actin was used as an internal control. ( B ) Increased levels of phosphorylated YAP after PTPN12 overexpression were noted. Protein lysates from A were subjected to Western blot analysis of PTPN12, YAP-pS217, and YAP. ( C ) The suppression of YAP-induced increased cell proliferation by PTPN12. MCF10A-GFP (WPI vector) and MCF10A-YAP-PTPN12 in the absence or presence of Dox were subjected to cell proliferation analysis. ( D ) The suppression of TAZ-induced increased cell proliferation by PTPN12. MCF10A-GFP (WPI vector) and MCF10A-TAZ-PTPN12 in the absence or presence of Dox were subjected to cell proliferation analysis. The mean and standard deviation (S.D.) of the cell numbers in triplicate samples at each day were shown.

Article Snippet: Primary , YAP/TAZ , YAP/TAZ Rabbit mAb , 1:1000 , Cell Signaling.

Techniques: Over Expression, Expressing, Plasmid Preparation, Western Blot, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Identification of PTPN12 Phosphatase as a Novel Negative Regulator of Hippo Pathway Effectors YAP/TAZ in Breast Cancer

doi: 10.3390/ijms25074064

Figure Lengend Snippet: Inhibition of YAP/TAZ-induced increased cell migration by PTPN12 in MCF10A mammary cells. ( A , C ) Wound healing analysis using Incucyte Zoom. MCF10A-GFP (WPI vector) and MCF10A-YAP/TAZ expressing Dox-inducible PTPN12 cells in the absence or presence of Dox (+PTPN12) were seeded into 96-wells. After making the wounds, cell migration was monitored as a percentage of wound closure using Incucyte Zoom for 72 h. ( B , D ) Images from ( A ) showing wound closure at 0 h and 48 h. MCF10A-GFP was used as a control for both MCF10A-YAP/PTPN12 and MCF10A-TAZ/PTPN12. Scale bar, 300 μm.

Article Snippet: Primary , YAP/TAZ , YAP/TAZ Rabbit mAb , 1:1000 , Cell Signaling.

Techniques: Inhibition, Migration, Plasmid Preparation, Expressing

Journal: Cell reports

Article Title: The calcium channel TRPC6 promotes chemotherapy-induced persistence by regulating integrin α6 mRNA splicing

doi: 10.1016/j.celrep.2023.113347

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-TAZ , BD-Pharmingen , Cat# 560235; RRID: AB_1645338.

Techniques: Virus, Derivative Assay, Recombinant, Mutagenesis, Bacteria, Sequencing, CRISPR, Plasmid Preparation, Software